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Journal : BIOTROPIC

Analisis Kadar Glukosa, Hemoglobin, dan Kolesterol Mencit (Mus Musculus) Setelah Diinduksi Aspartam Esti Tyastirin; Risa Purnamasari; Irul Hidayati; Eva Agustina; Moch. Irfan Hadi; Nova Lusiana; Mei Lina Fitri Kumalasari
Biotropic : The Journal of Tropical Biology Vol. 2 No. 2 (2018): Biotropic, Volume 2, Nomor 2, 2018
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2018.2.2.119-125

Abstract

Aspartame has a lower calorie rating than other sweeteners. for example, cyclamate, lactose, saccharin, fructose and maltosa. Although it has low calorie, aspartame have 60 times sweeter than sugar (sucrose). Induction of aspartame can cause elevated levels of blood glucose and damage to the pancreatic β cells of mice. The purpose of this study was to analyze the dosing of aspartame levels; 13 mg/Kg body weight, 39 mg/Kg and 78 mg/Kg and those effect to the levels of glucose, cholesterol and haemoglobin of female mice (Mus musculus). The methode of research laboratory experiments using a complete randomized design (RAL). This study was using 24 mice with 4 treatment groups. Mice were given aspartame during surgical treatment, mice were made to perform measurement of Hb levels, glucose levels and cholesterol levels of neonatal mice. Data analysis using the Kruskal Wallis because Gaussian data but not homogeneous. The results showed an increase in the levels of glucose and cholesterol levels, but statistically there is no difference in glucose levels (p = 0,087), Hb (p = 0,899) and cholesterol (p = 0,168) in mice (Mus musculus) who were given variations of the dose of aspartame.
Keanekaragaman Serangga pada Tanaman Akasia (Acacia nilotica) Di Savana Bekol Taman Nasional Baluran Situbondo Jawa Timur Saiful Bahri; Nirmala Fitria Firdhausi; Esti Tyastirin; Intan Ahmad
Biotropic : The Journal of Tropical Biology Vol. 6 No. 1 (2022): Biotropic, Volume 6 Nomor 1, 2022
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

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Abstract

Baluran National Park Situbondo have function to field study, and plays a role as the ecotourism area. Bekol savanna is part of Baluran National Park which has been invaded by Acacia (A. nilotica) plants. The aim of this study was to determine the diversity and abundant of the insects in A. nilotica in Bekol savannas park, Baluran national park. The research method has been employed with the make plots in the savannas dimensions of 20 x 20 m. Insect was collected by flying trap without antractan for four weeks. Insect identification was carried out at Entomology Laboratory SITH ITB. Diversty index was analyzed by Shannon’s index and abundant index with the Simpson’s index. The results showed that the 527 insects caught consisted of 5 insect orders that lived on A. nilotica in the Bekol Savanna . Diversity of insect have the range medium from H= 1,73186 – 2,29266. Insect diversity has high in the plot III and low from plot II. Abundant index was known that the familia Formicidae is dominance species with Polyrhachis sp=45,77465 % plot I, Crematogaster sp= 62,2807% plot II, Crematogaster sp= 45,45455% plot III and plot IV Crematogaster sp= 38,67925%. The low of diversity index because just many insect could live in the A. nilotica and some species likes Caryedon serattus, Zyras compressicornis, Crematogaster sp and Trigona lavaiceps was collected from four site reseach. The abundant of familia Formicidae because they have mutuallystic symbiosis with the A. nilotica. From the result we could conclude that diversity and abundant insect dependent to the plant and interaction have done.
Identifikasi Bakteri Toleran terhadap Logam Berat Pb yang Diisolasi dari Air dan Sedimen di Sungai Porong, Sidoarjo Nur Rokhmatul Lailiya; Misbakhul Munir; Esti Tyastirin; Eko Teguh Pribadi; Hanik Faizah
Biotropic : The Journal of Tropical Biology Vol. 6 No. 2 (2022): Biotropic, Volume 6 Nomor 2, 2022
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

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Abstract

Porong River is an active river located in the Sidoarjo region, East Java which has been polluted by the Lapindo mudflow containing various chemical compounds, one of which is lead (Pb) heavy metal. Isolation of bacteria in the environment contaminated with Pb allows bacteria that have the potential as bioremediation agents to grow. The purpose of this study was to determine the content of heavy metal Pb and isolate and identify lead (Pb) tolerant bacteria in water and sediment at Porong river, Sidoarjo. Sampling of water and sediment was carried out at 2 sampling points based on the presence of Lapindo mud waste flow. Analysis of the heavy metal content of Pb was carried out using the Atomic Absorption Spectrophotometer (AAS). Bacterial isolation was carried out on NA media containing 10 ppm Pb of heavy metal and incubated for 24 hours at 300C. Bacterial isolates were identified based on the macroscopic and microscopic properties and biochemical tests. The results of the heavy metal content of Pb at sampling point 1 in water was 0.81 ppm, and in sediment was 0.98 ppm while at sampling point 2 in water was 2.93 ppm, and in sediment was 3.88 ppm. The results of bacterial identification obtained as many as 6 bacterial isolates belonging to 3 genera of bacteria including Bacillus, Acinetobacter and Pseudomonas.