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All Journal Jurnal Ilmiah Farmasi Journal of Biomedicine and Translational Research Biota Jurnal Sains dan Teknologi Pangan Science and Technology Indonesia Jurnal Biologi Tropis Martabe : Jurnal Pengabdian Kepada Masyarakat Hearty : Jurnal Kesehatan Masyarakat Jurnal Kreativitas PKM Jurnal Ilmu Kefarmasian Indonesia Jurnal Abdimas PHB : Jurnal Pengabdian Masyarakat Progresif Humanis Brainstorming Jurnal Abdimas Mahakam Majalah Farmasetika CERATA Jurnal Ilmu Farmasi Jurnal Pembelajaran dan Biologi Nukleus Jurnal Ners Jurnal Riset Kefarmasian Indonesia HSG (Health Science Growth) Journal Janaka : Jurnal Pengabdian Masyarakat Lumbung Farmasi : Jurnal Ilmu Kefarmasian Indobiosains EEICT (Electric, Electronic, Instrumentation, Control, Telecommunication) Jurnal Ilmiah Wahana Pendidikan Jurnal Pijar MIPA Jurnal Farmasetis Journal of Pharmaceutical and Sciences. Parapemikir Jurnal Ilmiah Farmasi Media Farmasi CERATA Jurnal Sains dan Kesehatan Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Ahsanal Kasasiah
Universitas Singaperbangsa Karawang
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Journal : Biota

 1 
Comparison of Two PCR Primer Sets for In-House Validation of GHSR Gene Variation Detection Employing Artificial Recombinant Plasmid Approach Ahsanal Kasasiah; Jekmal Malau; Sekar Andjung Tresnawati; Priscinya Christiana Debora; Nur Komala Fitri; Saarah Hamidah Asmara Indratno; Asman Hitopik; Eriyanti Astika; Anisa Aula Rahma; Fikri, Al Mukhlas
Biota Vol 10 No 2 (2024): Jurnal Biota 2024
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v10i2.21166

Abstract

Stunting is a significant global public health problem caused by long-term dietary deficits that affect many children worldwide. Both environmental and genetic factors, including variants in the GHSR gene, play a crucial role in stunted growth. This study used an artificial recombinant plasmid DNA method to evaluate two primer set combinations for identifying DNA variants in the GHSR gene. Selecting suitable primer sets for identifying GHSR genetic variants linked to stunting is essential, as evidenced by PCR and sequencing techniques. The target gene, based on the GHSR reference sequence, consists of eight DNA variations (ΔQ36, G57G, P108L, L118L, R159R, C173R, D246A, and A277P). A recombinant plasmid was created by inserting a 1000 bp fragment of the GHSR gene into the pUC57 backbone. Primer sets were chosen based on their capacity to amplify these eight genetic variations and were optimized and validated using PCR methods. PCR and bi-directional sequencing verified the existence of surrounding DNA and specific single nucleotide variants (SNVs). In our study, we discovered four changes in the DNA sequence (R159R G>A, C173R T>C, D246A A>C, and A277P G>C) using the E1_F2/E1_R3 primer pair. Additionally, a new combination of primers (E1_F1/E1_R3) effectively detected seven DNA sequence mutations (ΔQ36 del CAG, G57G C>T, P108L C>T, L118L C>T, R159R G>A, C173R T>C, and D246A A>C). We have developed a new combination of forward and reverse primers to identify seven SNVs in the GHSR gene, which could serve as a diagnostic tool in clinical laboratory settings.
Co-Authors Adi Pancoro Adinda Christianti Suparno Adrian Hartanto, Adrian Aida, Fitri Ainaputri , Aliza Salsabila Ainaputri, Aliza Salsabila Aliya Azkia Zahra Anisa Aula Rahma Anisa Aula Rahma Annajla, Fathina Aprianti, Endeh Aprillia , Cantika Aprillia, Cantika Apriyanti , Endeh Asman Hitopik Astika, Eriyanti Cartas Cartas Dadang Sumardi Damara, Dandy Satria Daniel Joko Wahyono Devi - Ratnasari Devi Ratnasari Devi Ratnasari Dinda Nugrahan Eriyanti Astika Fika Setra Rikantara Fikri, Al Mukhlas Fuji Ayu Diniarti Hasna, Vina Luthfiana Hendiana, Siti Nurazizah Aghisna Hermosaningtyas, Anastasia Aliesa Hitopik, Asman Indah Laily Indah Laily Hilmi Indratno, Saarah Hamidah Asmara insani abadi bangsa Irgi, Khaeru Irwansyah, Silvana Lestari Jekmal Malau Jekmal Malau Lely Sulfiani Saula Lestari, Agatha Nabilla Malau , Jekmal Mally G Sholih Mally Ghinan Sholih Manalu, Rosario Trijuliamos Marsah Rahmawati Utami Meilani, Nanda Diva Meisya Diffa Amalia Putri Meliana Sari, Meliana Muhammad Naufal Nurhadi Hidayat Muhareva Raekiansyah Nabila Rubinadzari Nugraha , Afif Tri Nugraha, Afif Tri Nur Khafipah Nur Komala Fitri Nuraeni Putri Nurhadis, Nurhadis Nurzamani, Salsabila Priscinya Christiana Debora Rahmasari, Ratika Ratnasari, Devi Rivandia Listi Rizky Abdul Majid Rosa Arum Rusdiawan, Fairuz Octora Aulia Rusmayanti, Agustina Saarah Hamidah Asmara Indratno Salman Salman Saryono Sekar Andjung Tresnawati Septi, Annisa Frastica Septiani, Dia Sholih, Mally Ghinan Siboro , Dewi Pratiwi Purba Siboro, Dewi Pratiwi Purba Siti Rohmah Sophy Wulandari Sudarjat, Hadi Sulastri Sulastri Syeli Syawaliyah Urbaningrum, Lestari Mahardika Vesara Ardhe Gatera Zahra, Aliya Azkia Zahro, Aurora Fatimatuz