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All Journal Jurnal Ilmiah Farmasi Journal of Biomedicine and Translational Research Biota Jurnal Sains dan Teknologi Pangan Science and Technology Indonesia Jurnal Biologi Tropis Martabe : Jurnal Pengabdian Kepada Masyarakat Hearty : Jurnal Kesehatan Masyarakat Jurnal Kreativitas PKM Jurnal Ilmu Kefarmasian Indonesia Jurnal Abdimas PHB : Jurnal Pengabdian Masyarakat Progresif Humanis Brainstorming Jurnal Kesmas dan Gizi (JKG) Majalah Farmasetika CERATA Jurnal Ilmu Farmasi Jurnal Pembelajaran dan Biologi Nukleus Jurnal Ners Jurnal Riset Kefarmasian Indonesia HSG (Health Science Growth) Journal Janaka : Jurnal Pengabdian Masyarakat Lumbung Farmasi : Jurnal Ilmu Kefarmasian Indobiosains EEICT (Electric, Electronic, Instrumentation, Control, Telecommunication) Jurnal Ilmiah Wahana Pendidikan Jurnal Pijar MIPA Jurnal Farmasetis Journal of Pharmaceutical and Sciences. Parapemikir Jurnal Ilmiah Farmasi Media Farmasi CERATA Jurnal Sains dan Kesehatan Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia PharmaCine: Journal of Pharmacy, Medical and Health Science Jurnal Abdimas Mahakam
Ahsanal Kasasiah
Universitas Singaperbangsa Karawang
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Dentistry Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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Journal : Science and Technology Indonesia

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Advancements in Real-Time PCR Technologies: A Comprehensive Review of Probe-Based and Non-Probe-Based Assays for Molecular Diagnostics Malau, Jekmal; Zahro, Aurora Fatimatuz; Zahra, Aliya Azkia; Kasasiah, Ahsanal; Meilani, Nanda Diva; Damara, Dandy Satria; Lestari, Agatha Nabilla; Saryono; Wahyono, Daniel Joko
Science and Technology Indonesia Vol. 10 No. 3 (2025): July
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.3.660-677

Abstract

The decision between probe-based and non-probe-based qPCR assays is crucial, influenced by diagnostic goals and sample characteristics. This review provides an in-depth evaluation of these two assay types, analyzing their principles, strengths, drawbacks, and applications. A thorough review of the literature, primarily sourced from PubMed, was undertaken to explore prominent assay systems, including TaqMan, KASP, rhAmp, HRM, and SYBR Green. Probe-based qPCR assays, exemplified by TaqMan and rhAmp, are distinguished by their high specificity, aptitude for multiplex analysis, and reduced risk of false positives, making them highly suitable for SNP genotyping and pathogen detection. However, their elevated costs and intricate design requirements remain significant challenges. Conversely, non-probe-based assays, such as SYBR Green and HRM, present cost-effective alternatives with straightforward designs. HRM, in particular, is effective in identifying genetic variations like SNPs with remarkable sensitivity. Nonetheless, these methods are susceptible to non-specific amplifications, requiring careful optimization to maintain reliability. The selection of a suitable qPCR assay depends on various factors, including precision, affordability, and multiplexing capabilities, with applications spanning infectious disease detection and genetic disorder analysis. This review emphasizes the indispensable role of qPCR in molecular diagnostics while showcasing recent technological advances that aim to mitigate existing constraints and enhance diagnostic precision and accessibility.
Co-Authors Adi Pancoro Adinda Christianti Suparno Adrian Hartanto, Adrian Aida, Fitri Ainaputri, Aliza Salsabila Aliya Azkia Zahra Anisa Aula Rahma Anisa Aula Rahma Annajla, Fathina Aprianti, Endeh Aprillia , Cantika Aprillia, Cantika Apriyanti , Endeh Asman Hitopik Astika, Eriyanti Cartas Cartas Dadang Sumardi Damara, Dandy Satria Daniel Joko Wahyono Devi - Ratnasari Devi Ratnasari Devi Ratnasari Dinda Nugrahan Diniarti, Fuji Ayu Eriyanti Astika Fika Setra Rikantara Fikri, Al Mukhlas Fuji Ayu Diniarti Hasna, Vina Luthfiana Hendiana, Siti Nurazizah Aghisna Hermosaningtyas, Anastasia Aliesa Hitopik, Asman Indah Laily Indah Laily Hilmi Indratno, Saarah Hamidah Asmara insani abadi bangsa Irgi, Khaeru Irwansyah, Silvana Lestari Jekmal Malau Jekmal Malau Lely Sulfiani Saula Lestari, Agatha Nabilla Malau , Jekmal Mally G Sholih Mally Ghinan Sholih Manalu, Rosario Trijuliamos Marsah Rahmawati Utami Meilani, Nanda Diva Meisya Diffa Amalia Putri Meliana Sari, Meliana Muhammad Naufal Nurhadi Hidayat Muhareva Raekiansyah Nabila Rubinadzari Nugraha , Afif Tri Nugraha, Afif Tri Nur Khafipah Nur Komala Fitri Nuraeni Putri Nurhadis, Nurhadis Nurzamani, Salsabila Priscinya Christiana Debora Rahmasari, Ratika Ratnasari, Devi Reas Najla Anugrah Rivandia Listi Rizky Abdul Majid Rosa Arum Rusdiawan, Fairuz Octora Aulia Rusmayanti, Agustina Saarah Hamidah Asmara Indratno Salman Salman Saryono Sekar Andjung Tresnawati Septi, Annisa Frastica Septiani, Dia Sholih, Mally Ghinan Siboro , Dewi Pratiwi Purba Siboro, Dewi Pratiwi Purba Siti Nurazizah Aghisna Hendiana Siti Rohmah Sophy Wulandari Sudarjat, Hadi Sulastri Sulastri Syeli Syawaliyah Urbaningrum, Lestari Mahardika Vesara Ardhe Gatera Zahra, Aliya Azkia Zahro, Aurora Fatimatuz