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All Journal Jurnal Kesehatan Biota JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) Universa Medicina Journal of Health Science and Prevention AGRIPEAT AL-HAYAT: Journal of Biology and Applied Biology Biota Scripta Biologica Al-Ard: Jurnal Teknik Lingkungan Jurnal Riset Biologi dan Aplikasinya Indian Journal of Forensic Medicine & Toxicology Jurnal Biosense Nusantara Journal of Community Engagement Global Health Science (GHS) JURNAL BIOLOGI PAPUA Engagement: Jurnal Pengabdian Kepada Masyarakat Journal of Marine Resources and Coastal Management Folia Medica Indonesiana BIOTROPIC
Moch. Irfan Hadi
Universitas Islam Negeri Sunan Ampel Surabaya
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Phylogenetic Analysis and Mutation of Sars-Cov-2 in Bats in Karst Malang City, Indonesia Keman, Soedjajadi; Moch Irfan Hadi; Dedy Suprayogi; Yudied Agung Mirasa
JURNAL KESEHATAN LINGKUNGAN Vol. 16 No. 2 (2024): JURNAL KESEHATAN LINGKUNGAN
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkl.v16i2.2024.173-180

Abstract

Introduction: A group of people in China were hospitalized with an initial diagnosis of pneumonia of unknown cause. The patients were linked to a wholesale wet seafood and animal market in Wuhan, Hubei Province, China. The disease has spread to other provinces in China, Thailand, Japan, and South Korea in less than a month. SARS-CoV-2 was found to originate from bats. Therefore, this research aims to analyze SARS-CoV-2 mutation in bats in Malang Karst, Indonesia. Methods: Other bat body parts used as research samples include the brain, liver, kidneys, intestines, pancreas, fetus, blood, lungs, and ectoparasites. The samples were taken separately and placed in a container containing 10% PBF. For further analysis, we used RNA Extraction, Real-Time PCR, Sequencing, and CoV Gisaid mutation analysis software to analyze the sequencing data. Then, EMBL software will be used to analyze the phylogenetically. Results and Discussion: There was 1 sample that showed a positive result for Covid-19, namely the intestine of the Cynoptera brachyotis species. There were differences between SARS-CoV-2 in bats in Malang Karst in Indonesia compared to SARS-CoV from 2000 to 2019. The spike protein's receptor binding domain (RBD) is the most variable part of the coronavirus genome. Conclusion: From the research results, one positive sample was obtained using Real-Time PCR, and based on mutation analysis, mutations were found in SARS-CoV-2 against the SARS-CoV virus from 2000-2019. Further research is needed, especially regarding SARS-CoV-2 as a vaccine.
PENGARUH KOMPLEKS LINIER ALKYL BENZENE SULFONATE (LAS) DAN KADMIUM (Cd) TERHADAP PENINGKATAN AKUMULASI, ABSORBSI DAN TOKSISITAS KADMIUM (Cd) PADA Cyprinus carpio L. Hadi, Moch Irfan; Agustina, Eva; Andiarna, Funsu; Nadlir, Nadlir; Munir, Misbakhul
Al-Ard: Jurnal Teknik Lingkungan Vol. 4 No. 2 (2019): March
Publisher : Department of Environmental engineering, Faculty of Science and Technology, Islamic State University Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/alard.v4i2.477

Abstract

Deterjen merupakan salah satu hasil produk teknologi dengan menggunakan bahan kimia pengaktif permukaan (surfaktan) Alkyl Benzene Sulfonat (ABS) sebagai penghasil busa. ABS memiliki efek destruktif (buruk) terhadap lingkungan yakni sulit diuraikan oleh mikroorganisme, sehingga diganti dengan bahan aktif yang memiliki sifat lebih ramah lingkungan yaitu Linier Alkylbenzene Sulfonate (LAS). Tujuan penelitian adalah menganalisis pengaruh komplek Linier Alkyl Benzene Sulfonate (LAS) dan logam kadmium (Cd) dengan berbagai variasi konsentrasi terhadap absorbsi, akumulasi dan toksisitas logam kadmium (Cd) pada ikan mas (Cyprinus Carpio L). Sampel yang digunakan adalah ikan Cyprinus carpio L. dengan usia kurang lebih 3 bulan dengan asumsi bahwa ikan sudah berkembang secara seksual (mature). Cyprinus carpio L yang digunakan berkelamin jantan dengan panjang ±15cm dan berat badan ±100 gram. Penelitian ini menggunakan 6 variasi kelompok perlakuan dan 1 kelompok kontrol, maka dengan rumus diatas didapatkan r (ulangan) minimal 4 kali, maka dengan jumlah kelompokan 7 didapatkan besar sampel 28 ekor. Hasil penelitian ini adalah akumulasi lebih banyak ditemukan pada kelompok perlakuan Cd dan LAS. Kesimpulan dari hasil penelitian ini adalah didapatkan perbedaan akumulasi variasi perlakuan antara kelompok kontrol, Cd dan kombinasi LAS-Cd. Terjadinya akumulasi logam berat pada berbagai organisme dapat menimbulkan kerusakan pada lingkungan dan kesehatan pada manusia.
Viral Load And Cd4+ among Hiv/Aids Patients Receiving Antiretroviral Therapy In Jayawijaya District, Papua Province, Indonesia Widiyanti, Mirna; Hadi, Moch. Irfan; Adiningsih, Setyo; Natalia, Evi Iriani; Purba, Dedi Ananta
Folia Medica Indonesiana Vol. 58, No. 1
Publisher : Folia Medica Indonesiana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Hightlight:• Clinical conditions, CD4+ cell counts, and the viral copies number in the blood for AIDS/HIV were given antiretroviral therapy. • The profile of CD4+ levels and plasma viral load in HIV patients receiving antiretroviral therapy • The lower CD4+ cell counts and higher viral loads happen in HIV-infected's men. Abstract: Highly active antiretroviral therapy (HAART) is expected to reduce human immunodeficiency virus (HIV) morbidity and mortality. Antiretroviral therapy in HIV patients is given based on clinical conditions, CD4+ cell counts, and the number of viral copies in the blood. This study aimed to determine the profile of CD4+ levels and plasma viral load in HIV patients receiving antiretroviral therapy. This was a cross-sectional study conducted within six months at Voluntary Counseling and Testing (VCT) in Jayawijaya Hospital, Papua, Indonesia. The CD4+ levels were measured using CD4+ counter and viral plasma was checked using Polymerase Chain Reaction (PCR) for 90 patients. The results showed more female patients had a CD4+ level <200 cells/mm3, a higher number of copies of the virus in the blood plasma, and stages of disease 3 and 4. Statistically, there was a significant relationship between CD4+ levels and gender with a p-value = 0.00. HIV-infected males were more likely to have lower CD4+ cell counts and higher viral loads than females.
A community-based participatory model for integrating lobster aquaculture into coastal tourism livelihoods Perdanawati, Rizqi Abdi; Mauludiyah; Nugraha, Dimas Rizal Wahyu; Hadi, Moch Irfan; Violando, Wiga Alif
Journal of Marine Resources and Coastal Management Vol. 6 No. 2 (2025)
Publisher : UIN Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/mrcm.v6i2.2500

Abstract

Bangsring Village, Banyuwangi Regency, Indonesia is a coastal tourism area with high ecological and economic potential; however, tourist visitation has declined significantly since 2020, highlighting the need for locally grounded and resilient livelihood innovations. This study aims to evaluate the feasibility and community relevance of lobster aquaculture as a tourism-supporting livelihood strategy using a Community-Based Participatory Research (CBPR) framework. The CBPR process was implemented through collaborative problem identification, participatory planning, joint environmental assessment, and community-led implementation involving the Pesona Bahari Community Group. Water quality parameters and site characteristics were jointly analyzed to inform technology selection. Based on these findings, a circular bottom (base) net-cage system was selected due to its suitability for moderate current conditions, benthic substrates, and its ability to mimic the natural habitat of lobsters. Program implementation included cage construction and deployment, seed stocking, daily maintenance using natural feed, and harvesting after approximately 100 days of rearing. The outcomes indicate increased community capacity in environmentally responsible lobster aquaculture practices and successful production of marketable-sized lobsters under local conditions. Beyond economic diversification, lobster farming activities also function as an educational and experiential attraction, strengthening the integration between aquaculture and coastal tourism. This study contributes a context-specific CBPR-based model for integrating small-scale lobster aquaculture with coastal tourism development. The approach demonstrates practical applicability and potential replicability for other coastal communities seeking sustainable livelihood diversification without compromising ecosystem integrity.
DETEKSI KONTAMINASI BAKTERI Escherichia coli PADA ALAT MAKAN DI BEBERAPA TEMPAT MAKAN DI KABUPATEN GRESIK Miftakhus Sa'adah; Hadi, Moch. Irfan
JURNAL BIOSENSE Vol 9 No 1 (2026): Edisi Januari 2026
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i1.6549

Abstract

Alat makan yang tidak bersih merupakan faktor penting dalam penularan penyakit bawaan makanan, penelitian ini bertujuan untuk mengevaluasi efektivitas praktik kebersihan alat makan di beberapa tempat makan di Kabupaten Gresik dengan mendeteksi keberadaan bakteri Escherichia coli. Keberadaan E. coli dijadikan indikator utama kontaminasi fekal dan praktik higiene yang buruk, di mana alat makan yang bersentuhan langsung dengan makanan dipersyaratkan tidak mengandung E. coli (0 koloni) sesuai Permenkes RI No. 1096 Tahun 2011. Metode yang digunakan adalah uji usap (swab) pada 30 sampel alat makan (sendok, piring, mangkok) dari 10 tempat makan berbeda (5 warung, 3 katering, 2 restoran). Sampel diuji di laboratorium menggunakan metode Angka Lempeng Total (ALT). Hasil menunjukkan bahwa semua 30 sampel alat makan memiliki hasil negatif dan tidak terdeteksi adanya kontaminasi bakteri Escherichia coli. Kondisi ini mengindikasikan bahwa prosedur pencucian dan sanitasi di tempat-tempat tersebut kemungkinan besar telah dilakukan dengan benar.
Analisis Kadar Glukosa, Hemoglobin, dan Kolesterol Mencit (Mus Musculus) Setelah Diinduksi Aspartam Esti Tyastirin; Risa Purnamasari; Irul Hidayati; Eva Agustina; Moch. Irfan Hadi; Nova Lusiana; Mei Lina Fitri Kumalasari
Biotropic : The Journal of Tropical Biology Vol. 2 No. 2 (2018): Biotropic, Volume 2, Nomor 2, 2018
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2018.2.2.119-125

Abstract

Aspartame has a lower calorie rating than other sweeteners. for example, cyclamate, lactose, saccharin, fructose and maltosa. Although it has low calorie, aspartame have 60 times sweeter than sugar (sucrose). Induction of aspartame can cause elevated levels of blood glucose and damage to the pancreatic β cells of mice. The purpose of this study was to analyze the dosing of aspartame levels; 13 mg/Kg body weight, 39 mg/Kg and 78 mg/Kg and those effect to the levels of glucose, cholesterol and haemoglobin of female mice (Mus musculus). The methode of research laboratory experiments using a complete randomized design (RAL). This study was using 24 mice with 4 treatment groups. Mice were given aspartame during surgical treatment, mice were made to perform measurement of Hb levels, glucose levels and cholesterol levels of neonatal mice. Data analysis using the Kruskal Wallis because Gaussian data but not homogeneous. The results showed an increase in the levels of glucose and cholesterol levels, but statistically there is no difference in glucose levels (p = 0,087), Hb (p = 0,899) and cholesterol (p = 0,168) in mice (Mus musculus) who were given variations of the dose of aspartame.
Identifikasi Senyawa Aktif dari Ekstrak Daun Jambu Air (Syzygium aqueum) dengan Perbandingan Beberapa Pelarut pada Metode Maserasi Eva Agustina; Funsu Andiarna; Nova Lusiana; Risa Purnamasari; Moch. Irfan Hadi
Biotropic : The Journal of Tropical Biology Vol. 2 No. 2 (2018): Biotropic, Volume 2, Nomor 2, 2018
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2018.2.2.108-118

Abstract

Daun jambu air (Syzygium aqueum) dikenal sebagai salah satu obat tradisional, untuk menyembuhkan beberapa infeksi akibat antigen berupa bakteri. Penelitian ini bertujuan untuk mengetahui kandungan senyawa aktif yang terdapat dalam daun jambu air dengan beberapa pelarut pada proses maserasi. Metode ekstraksi yang digunakan dalam penelitian ini adalah ekstraksi dingin berupa maserasi dengan pemilihan pelarut yakni metanol (CH3OH), etil asetat dan n-heksana. Uji fitokimia yang dilakukan adalah uji saponin, flavonoid, alkaloid dan triterpenoid/steroid. Dari uji yang dilakukan diketahui sampel ekstrak daun jambu air dengan pelarut metanol positif mengandung beberapa senyawa aktif berupa flavonoid, saponin, alkaloid dan triterpenoid. Ekstrak daun jambu air dengan pelarut etil asetat dan n-heksana positif mengandung beberapa senyawa aktif berupa flavonoid, alkaloid dan triterpenoid. Pelarut yang paling bagus untuk mendapatkan banyak senyawa aktif adalah metanol. Kata kunci : Jambu air (Syzygium aqueum), maserasi, senyawa aktif
Dogs (Canis lupus familiaris) as Virus Carrier in Indonesia Moch Irfan Hadi; Muhammad Yusuf Alamudi; Mei Lina Fitri Kumalasari; Sri Hidayati; Tatag Bagus Prakarsa; Eva Agustina; Muhamad Ratodi; Misbakhul Munir; Eko Teguh Pribadi; Hanik Faizah
Biotropic : The Journal of Tropical Biology Vol. 2 No. 2 (2018): Biotropic, Volume 2, Nomor 2, 2018
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2018.2.2.100-107

Abstract

A Virus is an individual that cannot be described as an animal or a plant. If animals and plants contain two nucleic acids (DNA and RNA), on the contrary, the virus only has one of them. These nucleic acids can stimulate a complete virus replication cycle. The virus can only replicate and live on a living host if the host is finally dead, then the virus will move on the cells that are still alive. The virus has genetic material which is a protective protein coat called a capsid. Viruses can infect various varieties of organisms, both eukaryotes (animals, plants, protists, and fungi) and prokaryotes (bacteria and archaea). The Virus infects bacteria known as bacteriophage (phage). The Virus can cause serious diseases for humans such as AIDS, HIV, rabies, etc. Dogs belonging to Canidae family are the sibling of wolves, foxes and raccoon dogs. Among all members of Canidae, dogs have the most closely related to wolves which are the ancestors of dogs. The Canidae family generally has a small elongated body, sharp ear and muzzle, sharp smelling, can run fast and can swim. Dogs are human best friends. While taking care of the dogs, they can be attacked by various diseases. The closeness of the relationship between humans and dogs raises the potential for disease transmission, especially zoonosis and pandemics viruses. Keywords: Canis lupus familiaris, Carier, Influenza, virus
Analisis Kariometric Akibat Pemberian Variasi Dosis Aspartam pada Perkembangan Fetus Mencit (Mus Musculus L.) Esti Novi Andyarini; Nova Lusiana; Ika Mustika; Estri Kusumawati; Sri Hidayati; Moch Irfan Hadi; Risa Purnamasari; Dedy Suprayogi
Biotropic : The Journal of Tropical Biology Vol. 3 No. 2 (2019): Biotropic, Volume 3 Nomor 2, 2019
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2019.3.2.86-95

Abstract

Aspartame is safe for consumption according to the prescribed ADI, but this artificial sweetener is not nutritious or does not contain calories so it is not recommended to be consumed during pregnancy. This study was to analyze cariometric effect of aspartame doses in the development of fetal mice (Mus muculus L.). The type of research used was experimental laboratory research on 24 adult mice with 4 treatment groups, control, aspartame dose 13 mg / Kg BB, 39 mg / Kg BB and 78 mg / Kg BB. Data was analyzed using SPSS 16 with one way ANOVA. Based on the results of the study there were changes in the number and weight of the fetus due to aspartame doses, there was no change in fetal length due of aspartame dose variations. Consumption of aspartame during pregnancy should be avoided because it can cause teratogenic effects. For further research, it is necessary to observe the morphology and histology of the fetus which is expressed by aspartame to determine the types of congenital abnormalities.
Kontaminasi Residu Pestisida Organofosfat di Dalam Tanaman Holtikultura Widia Purnamasari; Moch Irfan Hadi; Eva Agustina
Biotropic : The Journal of Tropical Biology Vol. 4 No. 2 (2020): Biotropic, Volume 4 Nomor 2, 2020
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2020.4.2.98-110

Abstract

Excessive use of organophosphate pesticides is becoming more common these days. This can affect the level of pesticide residues in farmers' crops. Excessive spraying of pesticides can result in environmental pollution both small and large scale. If excessive exposure to these pesticides can cause damage to the ecosystem. The purpose of this study was to determine the level of organophosphate pesticide residues found in horticulture plants. This research method is a systematic review, by analyzing various studies regarding the contamination of organophosphate pesticide residues in horticultural crops. There are 20 studies from within the country. The results obtained in the form of levels of pesticide residues that are still below the threshold of the BMR (Maximum Residue Limit) of pesticides determined by SNI. Two other studies show that no pesticide residue content was detected. Conclusion: This shows that the sample tested is still safe for consumption. Socialization and education about the doses of pesticide use and how to reduce levels of pesticides in horticultural crops are needed to reduce the risk of exposure to pesticides
Co-Authors Abdul Azis Abdul Muhid Adiningsih, Setyo Aditya Sukma Pawitra Aditya Sukma Prawira Agung, Tri Sunan AGUSTINA, EVA Albarkah, M. Rafly ANDYARINI, ESTI NOVI Annisa Inda Sanabila Asri Sawiji Atiqoh Zummah Ayudya Fitri Arifa Choiriyah, S.Si, Chumaidatul Corie Indria Prasasti Dedi Ananta Purba Dedy Suprayogi Dian Tugu Warsito Taufiq Eko Teguh Pribadi Esti Tyastirin Estri Kusumawati Eva Agustina Eva Agustina, Eva Evi Iriani Natalia Faizah, Hanik Fatih, M. Fachrul Funsu Andiarna Hanik Faizah Ika Mustika Irul Hidayati Izza Mahendra Kartika Dewi Kartika Dewi Khuliyah Candraning Diyanah Linggar Dwi Kinasih M Yusuf Alamudi M. Chusnan Ma'arif M. Yusuf Alamudi Mauludiyah Ma’arif, M. Chusnan Mei Lina Fitri Kumalasari Mei Lina Fitri Kumalasari Mei Lina Fitri Kumalasari Mei lina Fitri Kumalasari Mei Lina Fitri Kumalasari Mei Lina Fitri Kumalasari Miftakhus Sa'adah Mirna Widiyanti Mirna Widiyanti Mirna Widiyanti Mirna Widiyanti MISBAKHUL MUNIR Misbakhul Munir Misbakhul Munir Muhamad Ratodi Muhammad F. Musyaffa Muhammad Falikhul Musyaffa' Muhammad Yusuf Alamudi Muhammad Yusuf Alamudi Muhammad Yusuf Alamudi Muhammad Yusuf Alamudi Nadlir Nadlir Nadlir Nadlir, Nadlir Nanang Supriatna Natalia, Evi Iriani Nirmala Fitria Firdhausi Nova Lusiana Nugraha, Dimas Putra Wahyu Nugraha, Dimas Rizal Wahyu Nur Maulida Safitri Nusa, Raras Widya Perdanawati, Rizqi Abdi Puji Maryono Purba, Dedi Ananta Putra, Riswanda Rangga Risa Purnamasari Rizqiyah, Ziadatur Robayanto Robayanto Sanabila, Annisa Inda Setyo Adiningsih Setyo Adiningsih Sigit Ari Saputro Soedjajadi Keman Sri Hidayati Sri Hidayati Suhardi, Haidar Rafli Putra Syahrul Afifa Ratna Sari Tatag Bagus Prakarsa Teguh Taruna Utama Widia Purnamasari Wiga Alif Violando Yuanita Rachmawati Yudied Agung Mirasa