Article Per Year (5 Year)
p-Index From 2021 - 2026
8.556
P-Index
This Author published in this journals
All Journal Jurnal Veteriner Jurnal Riset Kesehatan Quagga Biology, Medicine, & Natural Product Chemistry Journal of Natural Sciences and Mathematics Research Jurnal Ilmiah Universitas Batanghari Jambi Jurnal SOLMA JURNAL KESEHATAN PERINTIS Prosiding Seminar Nasional Multidisiplin Jurnal Inovasi Farmasi Indonesia Abdimas Galuh: Jurnal Pengabdian Kepada Masyarakat Jurnal Abdimas Ilmiah Citra Bakti (JAICB) Indonesian Journal of Natural Science Education PHARMACOLOGY, MEDICAL REPORTS, ORTHOPEDIC, AND ILLNESS DETAILS (COMORBID) BIO-CONS: Jurnal Biologi dan Konservasi Gema Kesehatan Journal of Engineering Science and Technology Management Journal for Quality in Public Health Komunita: Jurnal Pengabdian dan Pemberdayaan Masyarakat Jurnal Kesehatan Metro Sai Wawai Jurnal Mahasiswa Kesehatan Jurnal Bioteknologi & Biosains Indonesia (JBBI) J-CEKI Joong-Ki Jurnal Bioteknologi & Biosains Indonesia
savitri, Lisa
Universitas Kadiri
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

Published : 73 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 73 Documents
Search

 4   5   6   7   8 
Assessment of Escherichia coli Contamination in Drinking Water from Refill Depots Savitri, Lisa; Kholis, Muhammad Akbar Nur; Prasetyawan, Fendy; Saristiana, Yuneka; Amanda, Cornelia; Mebung, Konradus Klala
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.443-446

Abstract

Refill Drinking Water Depots (DAMIU) have become a popular source of drinking water in Indonesia due to their affordability and accessibility. However, concerns about hygiene and microbial contamination persist, particularly in areas with limited health oversight. This study aimed to evaluate the microbiological quality of refilled drinking water in Kediri City by detecting the presence of Escherichia coli as an indicator of fecal contamination. A descriptive, cross-sectional design involving 22 depots selected based on hygiene criteria was employed. Microbiological analysis used the Most Probable Number (MPN) method, with confirmatory tests including IMViC and Eosin Methylene Blue (EMB) media. The results showed that 20 of 22 depots met the microbiological safety standard of 0 CFU/100 mL for E. coli as set by the Indonesian Ministry of Health and national standards (SNI 3554:2015). Two samples (5 and 7) tested positive for E. coli, likely due to environmental contamination, inadequate sanitation, and poor water handling practices. The positive samples demonstrated typical E. coli characteristics in IMViC (++-+) and gas formation in BGLB media. This study underscores the importance of routine surveillance, public health regulation enforcement, and improved hygiene practices in refilled water depots. Ensuring the microbiological safety of refilled drinking water is essential to prevent waterborne diseases such as diarrhea. The findings call for enhanced collaboration between local health offices and depot operators to maintain clean and safe drinking water standards across communities.
Immunomodulatory Activity of Chinese Betel (Peperomia pellucida L.) Extract on the Spleen Histopathology in a Murine Model of Gastroenteritis Savitri, Lisa; Prasetyawan, Fendy; Saristiana, Yuneka; Meri, Meri; Mebung, Konradus Klala; Amanda, Cornelia
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.447-452

Abstract

Gastroenteritis, a prevalent digestive disorder caused by various pathogens including Escherichia coli, remains a global health challenge with significant morbidity and mortality, particularly in low-income countries. The spleen, as a critical immune organ, is often compromised during systemic infections. Peperomia pellucida (L.) , a traditional medicinal herb, is known for its antimicrobial, anti-inflammatory, and antioxidant properties. This study investigates the histopathological effects of P. pellucida leaf ethanol extract on spleen tissue in mice induced with E. coli to model bacterial gastroenteritis. Thirty male Swiss mice were divided into six groups (n=5): normal control, negative control (aquades), positive control (Yakult), and three treatment groups receiving P. pellucida extract at 100, 300, and 500 mg/kg BW. After seven days of pretreatment, mice were orally infected with E. coli (1×10⁶ CFU/mL) for another seven days. Spleen tissues were harvested, fixed, and stained with hematoxylin-eosin for histopathological evaluation focusing on degeneration, necrosis, and PMN infiltration. Statistical analysis was performed using ANOVA followed by LSD tests. The highest dose of P. pellucida extract (500 mg/kg BW) significantly reduced spleen tissue damage, showing decreased degeneration (9.08%), necrosis (6.05%), and PMN infiltration (18.45%) compared to lower doses. The effect was comparable to the positive control. The ethanol extract of P. pellucida demonstrates a dose-dependent protective effect on spleen histopathology in E. coli-induced gastroenteritis in mice, supporting its potential as a natural antiseptic agent.
Histopathological Evaluation of Stomach Protection by Peperomia pellucida L. in Mice with Gastroenteritis Savitri, Lisa; Krissanjaya, Rochmad; Kasimo, Elfred Rinaldo; Juwita, Syntia Tanu; Prasetyawan, Fendy; Saristiana, Yuneka; Mebung, Konradus Klala; Amanda, Cornelia
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.535-540

Abstract

Gastroenteritis, a widespread condition characterized by inflammation of the stomach and intestines, poses significant health challenges globally. Conventional treatments primarily focus on symptomatic relief and do not address the underlying gastric mucosal damage. Peperomia pellucida L., a medicinal plant known for its anti-inflammatory and antioxidant properties, has been suggested to possess gastroprotective effects. This study aims to evaluate the histopathological effects of P. pellucida L. on gastric protection in a mouse gastroenteritis model. Male Swiss mice were divided into six groups, receiving different treatments, including the ethanol extract of P. pellucida at varying doses (100, 300, and 500 mg/kgBW), followed by induction of gastroenteritis with Escherichia coli. Histopathological analysis was conducted to observe tissue damage across the treatment groups, including necrosis and cell degeneration. The results revealed that P. pellucida L. exhibited significant gastroprotective effects, particularly at a dose of 500 mg/kgBW, reducing gastric mucosal necrosis and inflammation compared to the control groups. The plant’s secondary metabolites, including flavonoids, tannins, saponins, and alkaloids, contributed to the observed protective effects by enhancing mucus production, reducing gastric acidity, and promoting tissue repair. These findings suggest that P. pellucida L. could be an alternative treatment for gastroenteritis and related gastric conditions, offering a natural approach to managing gastric inflammation and mucosal protection.
Antibacterial Activity Test of Trump Extract Dendrophtoe petandra (L.) Miq. Against Pseudomonas aeruginosa in Vitro Savitri, Lisa; Alves, Silvina Sandra X.; Hilmi, Mochammad Hanif; Krissanjaya, Rochmad; Prasetyawan, Fendy; Juwita, Syntia Tanu
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.553-556

Abstract

Mistletoe (Dendrophthoe pentandra (L.) Miq.) is one of the plants found in Indonesia. The type of mistletoe commonly found is D. pentandra (L.) Miq. Apart from its parasitic nature, the leaves of D. pentandra (L.) Miq. has useful benefits as an antibacterial. Bacteria are divided into two groups based on their staining, namely Gram-positive bacteria and Gram-negative bacteria. Infectious diseases can be caused by several types of microorganisms in the bacterial group, including Pseudomonas aeruginosa. This study aims to determine the P. aeruginosa bacteria in the extract of lime mistletoe. This study used an experimental post-test only control research design with the disc diffusion method. This study used five concentrations, namely 20%, 40%, 60%, 80%, and 100%, and used distilled water as a negative control and ciprofloxacin as a positive control. The inhibition zone is determined by observing the clear zone.
Evaluation of Rational Antibiotic Use (RAU) in Cerebrovascular Accident (CVA) Patients in the ICU Using the Gyssens Criteria Prasetyawan, Fendy; Saristiana, Yuneka; Savitri, Lisa; Salmasfattah, Novyananda; Ilmi, Tsamrotul; Herman, Herman
PHARMACOLOGY, MEDICAL REPORTS, ORTHOPEDIC, AND ILLNESS DETAILS Vol. 4 No. 3 (2025): JULY
Publisher : Transpublika Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55047/comorbid.v4i3.1818

Abstract

Cerebrovascular accident (CVA) is a major cause of morbidity and mortality, often requiring intensive care unit (ICU) treatment. Patients with CVA are at high risk for secondary infections, which commonly lead to the use of antibiotics. This study aimed to evaluate the patterns and rationality of antibiotic use in CVA patients treated in the ICU of a regional hospital using the Gyssens method. A retrospective observational study was conducted involving 30 CVA patients who received antibiotics during their ICU stay. Data collected included patient demographics, comorbidities, types of antibiotics used, route of administration, combination patterns, and evaluation of prescription rationality based on the Gyssens criteria. The most frequently used antibiotic class was third-generation cephalosporins (60%), with ceftriaxone being the most prescribed (46.7%). All antibiotics were administered intravenously. Combination therapy was used in 20% of cases. Based on Gyssens categorization, only 33.3% of prescriptions were considered rational (category 0). The remaining 66.7% were irrational, including delayed initiation or discontinuation (13.3%), incorrect dosing (16.7%), unnecessarily broad-spectrum (10%), and no indication (6.7%). The study highlights a substantial proportion of irrational antibiotic use in ICU CVA patients, mainly due to suboptimal timing, dosing errors, and unnecessary broad-spectrum antibiotics. These findings underline the importance of antimicrobial stewardship and routine rationality assessments to ensure optimal antibiotic use and patient outcomes.
Edukasi Standar Penggunaan Air untuk Pembuatan Media Mikrobiologi pada Pemeriksaan Sediaan Kosmetik di PT. Triszie Lab Indonesia Savitri, Lisa; Kasimo, Elfred Rinaldo; Prasetyawan, Fendy; Saristiana, Yuneka; Mebung, Konradus Klala; Amanda, Cornelia
Abdimas Galuh Vol 7, No 2 (2025): September 2025
Publisher : Universitas Galuh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25157/ag.v7i2.18780

Abstract

Air merupakan komponen krusial dalam pembuatan media mikrobiologi, khususnya untuk pengujian sediaan kosmetik. Penggunaan air yang tidak memenuhi standar dapat menyebabkan kontaminasi, menurunkan validitas hasil uji, serta menghambat pertumbuhan mikroorganisme target. Oleh karena itu, laboratorium mikrobiologi wajib menggunakan air yang sesuai standar internasional seperti United States Pharmacopeia (USP) dan European Pharmacopoeia (EP), dengan pengawasan parameter seperti TOC, konduktivitas, dan cemaran mikrobiologi. PT. Triszie Lab Indonesia menyadari pentingnya edukasi mengenai standar mutu air, namun masih terdapat kesenjangan pengetahuan di antara personel laboratorium. Kegiatan pengabdian kepada masyarakat ini bertujuan memberikan pelatihan dan pendampingan terkait standar penggunaan air laboratorium. Metode yang digunakan meliputi observasi awal, pelatihan interaktif, penyusunan SOP, serta evaluasi melalui pre-test dan post-test. Hasil kegiatan menunjukkan peningkatan pemahaman teknis sebesar 50%, peningkatan praktik higiene, serta penerapan SOP yang sesuai standar USP dan WHO. Kegiatan ini juga mendorong perubahan budaya kerja dan kesadaran mutu di lingkungan laboratorium. Produk luaran seperti buku saku dan modul pelatihan mendukung keberlanjutan edukasi internal. Secara keseluruhan, kegiatan ini berkontribusi nyata terhadap peningkatan mutu pengujian mikrobiologi dan kesiapan laboratorium dalam menghadapi audit serta sertifikasi.
In Vitro Evaluation of the Antibacterial Properties of Lime Mistletoe (Dendrophthoe petandra (L.) Miq.) Extract Against Escherichia coli Savitri, Lisa; Mahardika, Juan Vega; Yanti, Novirma; Juwita, Syntia Tanu
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.641-644

Abstract

The increasing prevalence of antibiotic-resistant bacteria necessitates the exploration of alternative antimicrobial agents derived from natural sources. This study aimed to evaluate the in vitro antibacterial activity of lime mistletoe (Dendrophthoe petandra (L.) Miq.) extract against Escherichia coli ATCC 25922 using the disc diffusion method. Extract concentrations of 20%, 40%, 60%, 80%, and 100% were prepared alongside negative (distilled water) and positive (Cefadroxil) controls. Nutrient Agar (NA) media were inoculated with E. coli and discs impregnated with each concentration were placed on the media, followed by incubation at 37°C for 24 hours. The inhibition zones were measured to assess antibacterial activity. Results demonstrated a concentration-dependent increase in antibacterial efficacy, with inhibition zone diameters ranging from 8.3 mm at 20% extract to 20.3 mm at 100% extract concentration. The highest concentration’s inhibition zone was comparable to that of Cefadroxil (22.5 mm). No inhibition was observed in the negative control. Statistical analysis confirmed significant differences between treatment groups (p < 0.05). The antibacterial activity of lime mistletoe extract is likely due to its bioactive phytochemicals such as flavonoids and phenolics, which are known to disrupt bacterial cell membranes and inhibit microbial growth. These findings suggest that Dendrophthoe petandra extract holds potential as a natural antibacterial agent, providing an alternative to conventional antibiotics amid growing resistance issues. Further studies involving isolation of active compounds, toxicity evaluation, and in vivo testing are recommended to validate its clinical applicability.
Isolation and Identification of Dermatophytes from Toenails and Interdigital Spaces of Students Using Czapek Yeast Extract Agar Savitri, Lisa; Ihsan, Kharisul; Krissanjaya, Rochmad; Kasimo, Elfred Rinaldo; Yanti, Novirma; Hilmi, Mochamad Hanif
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.795-798

Abstract

Dermatophytosis remains a common superficial fungal infection affecting nails and interdigital spaces, with Trichophyton rubrum and T. mentagrophytes as the predominant etiologic agents. The recent emergence of terbinafine-resistant T. indotineae has raised new challenges in diagnosis and treatment. Although Sabouraud Dextrose Agar is the conventional medium for fungal culture, alternative media such as Czapek Yeast Extract Agar (CYA) may provide additional advantages for dermatophyte isolation and morphological identification. This study aimed to isolate and identify dermatophytes from toenails and interdigital spaces of university students using CYA medium and to evaluate its applicability in dermatophyte culture. A cross-sectional descriptive study was conducted among 100 university students aged 18–24 years. Specimens were collected from toenails (n = 60) and interdigital spaces (n = 40). Direct microscopy was performed using 20% KOH preparation. Samples were cultured on CYA supplemented with chloramphenicol and cycloheximide, and incubated at 28 ± 2 °C for up to 21 days. Fungal isolates were identified based on macroscopic and microscopic morphology. Data were analyzed descriptively, and associations were tested using the chi-square test. Fungal elements were detected in 65% of samples by KOH examination, and dermatophyte growth was confirmed in 52% of cultures. The most frequently isolated species were T. rubrum (28%) and T. mentagrophytes (18%). Notably, T. indotineae was detected in 5% of samples. No significant difference was found between toenail and interdigital isolates (p = 0.26). Dermatophytes are prevalent among university students, with T. rubrum as the dominant species. The detection of T. indotineae highlights its emerging role in young populations. CYA proved effective for dermatophyte isolation and may serve as an alternative culture medium in academic and diagnostic laboratories.
Effect of Ciprofloxacin Administration on Gastric Histopathological Changes in Mice Savitri, Lisa; Ihsan, Kharisul; Krissanjaya, Rochmad; Kasimo, Elfred Rinaldo; Yanti, Novirma; Hilmi, Mochamad Hanif
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.873-878

Abstract

Ciprofloxacin is a fluoroquinolone antibiotic widely used in clinical and veterinary practice. While effective against a broad range of bacterial infections, several studies have reported potential adverse effects on the gastrointestinal tract, particularly the gastric mucosa. This study aimed to evaluate the histopathological changes in the gastric mucosa of mice following ciprofloxacin administration at different doses. A total of 24 male mice were randomly divided into four groups: control, low-dose ciprofloxacin, moderate-dose ciprofloxacin, and high-dose ciprofloxacin. Treatment was administered orally for 14 days. Gastric tissues were collected and examined histologically using hematoxylin-eosin staining. Histopathological analysis revealed dose-dependent mucosal alterations. The control group showed intact gastric architecture, while the low-dose group exhibited mild epithelial erosion. The moderate-dose group presented with mucosal disruption and inflammatory infiltration, and the high-dose group demonstrated severe ulceration, edema, and mucosal thinning. These findings indicate progressive gastric injury with increasing ciprofloxacin exposure. Ciprofloxacin administration induces dose-dependent gastric mucosal injury in mice, consistent with fluoroquinolone-related gastrointestinal toxicity. Caution is warranted in prolonged or high-dose therapy, and further studies are needed to explore the underlying mechanisms and potential gastroprotective interventions.
Onychomycosis in Students: An Overview of Nail Care Practices and Personal Hygiene Savitri, Lisa; Ihsan, Kharisul; Krissanjaya, Rochmad; Kasimo, Elfred Rinaldo; Yanti, Novirma; Hilmi, Mochamad Hanif
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1015-1019

Abstract

Onychomycosis is a common nail disorder caused by dermatophytes, yeasts, and non-dermatophyte molds, with increasing prevalence among young adults. Poor nail care and personal hygiene habits are essential predisposing factors that contribute to infection. Despite its clinical relevance, data on onychomycosis in student populations remain limited. A cross-sectional study was conducted among 150 undergraduate students from the Faculty of Health Sciences, aged 18–25 years. Nail specimens were collected from individuals presenting with suspected onychomycosis. Direct microscopy was performed using a 20% potassium hydroxide (KOH) preparation, and samples were cultured on Sabouraud Dextrose Agar supplemented with chloramphenicol and cycloheximide. Fungal isolates were identified based on macroscopic and microscopic characteristics. Sociodemographic data, nail care habits, and hygiene practices were collected through structured questionnaires. Data were analyzed descriptively, and associations were assessed using chi-square tests. Onychomycosis was confirmed in 27 students (18%). Dermatophytes were the most frequent isolates, with Trichophyton rubrum (9%) and T. mentagrophytes (5%) predominating, followed by Candida albicans (3%) and Aspergillus spp. (1%). Risk factors significantly associated with infection included irregular nail trimming (p = 0.01), sharing of nail clippers (p = 0.03), and prolonged use of closed footwear (p = 0.02). No significant associations were observed with gender or age. Onychomycosis is prevalent among university students, with dermatophytes as the leading causative agents. Inadequate nail care and poor hygiene practices play a critical role in the occurrence of diseases. Health education and preventive interventions should be prioritized to reduce infection rates in young adults. Further studies employing molecular methods are recommended to refine species identification and improve epidemiological insights.
Co-Authors Afrilla, Rananda Agung Dwi Wahyu Widodo Agustanti, Khalimatus Nur Eka Alimansur, Moh Alves, Silvina Sandra X. Amanda, Cornelia Aminah Alfiani Ana Retnowati Antoro, Ester Lianawati Arif Budi Setiawan Besan, Emma Jayanti Datin An Nisa Sukmawati Dhony Hermanto Edda, Ilda Rambu Eka Wahyuningtiyas Elfred Rinaldo Kasimo Elfred Rinaldo Kasimo Elfred Rinaldo Kasimo Elfred Rinaldo Kasimo Elfred Rinaldo Kasimo, Elfred Rinaldo Elly Megasari Ester Lianawati Antoro Ester Lianawati Antoro Ester Lianawati Antoro Ester Lianawati Antoro Evi Laela Sari Farabi, Meka Faizal Fendy Prasetyawan Fitri, Devi Nor Freitas, João Gregório Freitas, Maria Do Carmo Da Costa Herman Hilmi, Mochamad Hanif Hilmi, Mochammad Hanif Ida Septika Wulansari Ida Septika Wulansari Ida Septika Wulansari Ida Septika Wulansari Ihsan, Kharisul Indrayanti, Dian Iza Dwi Muslikha Juan Vega Mahardhika Juwita, Syntia Tanu Kadir, Mujtahid Bin Abd Kadir, Mujtahid Bin And Kasimo, Elfred Khalimatus Nur Eka Agustanti Kharisul Ihsan Kholis, Akbar Nur Kholis, Muhammad Akbar Nur Laili, Nur Fahma La’ally Maula Lian Pandu Farendra Loviana, Novi Mahardika, Juan Vega Maria Do Carmo Da Costa Freitas Masyhuri, Aga Adi Mebung, Konradus Klala Megasari, Elly Meri, Meri Mika Tri Kumala Swandari, Mika Tri Kumala Mosse1, Yosep Muhammad Nurul Fadel Muslikha, Iza Dwi Neni Probosiwi Novyananda Salmasfattah Novyananda Salmasfattah Nur Fahma Laili Nur Fahma Laili Nurul Ismillayli Ornai, Andre Mario Sequeira Alves Rasyadan Taufiq Probojati Renaldi Umbu Ndamung Kale Restyana, Anggi Restyana, Anggy Retnowati, Ana Rochmad Krissanjaya Rochmad Krissanjaya Salmasfattah, Novyananda Sanjaya, Rochmad Kris Shinta Mayasari Shinta Mayasari Sueb Sugeng Santoso Syntia Tanu Juwita Syntia Tanu Juwita Syntia Tanu Juwita Syntia Tanu Juwita Syntia Tanu Juwita Tiyas, Eka Wahyuning Tridoso Sapto A Tsamrotul Ilmi Willy Sandhika Wulansari, Ida Septika Yanti, Novirma Yosep Mosse yunadi, frisca dewi Yuneka Saristiana