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Ratih Puspita Febrinasari

Universitas Sebelas Maret

Author-ID : 265413

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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Hepatoprotective Effect of Ethanolic Extract of Pegagan Leaves (Centella asiatica L. Urban) on Histological Damage of Hepatocyte on Mice Model induced by Parasetamol Naila Shofwati Putri; Suyatmi .; Ratih Puspita Febrinasari
Nexus Biomedika Vol 4, No 1 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Background: Pegagan leaves (Centella asiatica L. Urban) contain antioxidative compounds that can protect hepatocytes from free radicals. The aim of this research was to know the hepatoprotective effect of ethanolic extract of pegagan leaves (Centella asiatica L. Urban) on histological damage of hepatocyte on mice model induced by paracetamol. Methods: This study was an experimental laboratory with the post test only control group design. Samples were 30 male Swiss webster mice, with 2-3 months old and 20 gr body weight divided into 5 groups equally. KN and KI were given distilled water for 14 days. In addition, KI was also given paracetamol in dose 5.07 mg/20 gbw on the 12th, 13th, 14th day. KP1, KP2, and KP3 were given ethanolic extract of pegagan leaves in dose 5 mg, 10 mg, and 20 mg per 20 gbw respectively for 14 days and paracetamol in dose 5.07 mg per 20 gbw on the 12th, 13th, 14th day. On the 15th day, mice were sacrificed and their livers were taken for preparation with paraffin block method and stained with HE. The features of histological damage of hepatocyte were assessed by counting the numbers of nucleus damage of hepotocyte (pyknosis, karyorrhexis, karyolysis) of 100 cells in the sentrolobular zone. The data were analyzed by One-way ANOVA test and Post Hoc LSD test. \ Results: The means of histological damage of hepatocyte were 16.08 5.160; 66.00 3.275; 44.58 5.583; 31.67 2.188; 26.17 3.810 for KN, KI, KP1, KP2, KP3 respectively. The result of One-Way ANOVA test was *p = 0.000. The result of Post Hoc LSD test showed a significant difference between five groups, *p = 0.000 for KN-KI, KN-KP1, KN-KP2, KN-KP3, KI-KP1, KI-KP2, KI-KP3, KP1-KP2, KP1-KP3 and *p = 0.002 for KP2-KP3. Conclusion: The ethanolic extract of pegagan leaves can prevent histological damage of hepatocyte on mice model induced by paracetamol. Keywords: ethanolic extract of pegagan leaves, paracetamol, histological damage of hepatocyte

Efek Nefroprotektif Ekstrak Daun Sirsak (Annona muricata L.) pada Mencit Model yang Diinduksi Parasetamol Nur Hidayah; . Suyatmi; Ratih Puspita Febrinasari
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Soursop Leaf (Annona muricata L.) contains active compounds that have antioxidant effects consisting of flavonoid, alkaloid, tannin, triterpenoid and steroid. The aim of this research is to know the protective effect of Soursop Leaf Extract on the renal histological damage of mice induced by paracetamol Methods: This study was experimental research with the post test only controlled group design. Samples were 30 male Swiss webster mice, 2-3 months old and 20 g of body weight. Samples were divided randomly into 5 groups, each group consisted of 6 mice. The normal group (KN) and negative control group (KKn) were given distilled water for 14 days. KP1, KP2, and KP3 were given soursop leaf extract in dose 5.6 mg, 11.2 mg, and 22.4 mg per g of body weight of mice, respectively, for 14 days. Paracetamol in dose 5.07 g per 20 g body weight of mice were given to KKn, KP1, KP2, and KP3 on the 12th ,13th ,14th days. On the 15th day mice were sacrificed and then renal organ were taken for histological preparation and stained with HE. Renal histological features were assessed based on quantifying of necrotic cells (pyknosis, karyorrhexis, karyolysis) of 50 epithelial cells from proximal tubule of right and left renal organ. Data were analyzed using One-Way ANOVA test and continued with Post Hoc (LSD) test. Results: The means of necrotic cells for KN, KKn, KP1, KP2, and KP3 were 8.17, 38.58, 26.92, 21.08 and 11.67, respectively. The One-Way ANOVA test showed a significant difference of renal damage score in five groups with p = 0.000. The Post Hoc LSD test also showed significant difference among five groups, p = 0.000. Conclusion: Soursop leaf extract can prevent the histological damage of renal cells on mice induced by paracetamol. Keywords: Soursop leaf extract, paracetamol, renal histological damage

Efek Hepatoprotektif Ekstrak Daun Sirsak (Annona muricata L.) Terhadap Kerusakan Histologis Sel Hepar Mencit (Mus musculus) yang Diinduksi Parasetamol Riris Arizka Wahyu Kumala; . Suyatmi; Ratih Puspita Febrinasari
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Background: Soursop leaves (Annona muricata L.) contains alkaloid, flavonoid, saponin, triterpenoid, and steroid that are potent antioxidant to protect the liver from free radicals. The aim of this research was to know the hepatoprotective effect of soursop leaves extract toward liver histological damage of mice (Mus musculus) induced by paracetamol. Methods: This was an experimental laboratory research with the post test only controlled group design. Samples were 30 male Swiss Webster mice with 2-3 months old and 20g of weight taken by incidental sampling. They were divided randomly into 5 groups, namely kelompok kontrol negatif (KKn), kelompok kontrol (KK), kelompok perlakuan 1 (KP1), kelompok perlakuan 2 (KP2), and kelompok perlakuan 3 (KP3). KKn and KK were given aquadest for 14 days. Soursop leaves extract were given to KP1, KP2, KP3 in dose 5.6 mg, 11.2 mg, and 22.4 mg/20gbw of mice respectively for 14 days. Paracetamol in dose 5.07 g/20gbw were given to KK, KP1, KP2, and KP3 on the 12th, 13th, 14th day. On the 15th day, mice were sacrificed and their livers were taken for histological preparation with paraffin block method and HE staining. Liver histological features were assessed based on quantifying of pyknosis, karhyorhexis, and karyolysis of each 100 cells on the 3rd zone. Data were analyzed using the One-Way ANOVA test (? = 0,05) and continued with Post Hoc (LSD) test (? = 0,05). Results: The results of One-Way ANOVA test showed a significant difference of liver damage score among groups, *p = 0.000. Results of LSD test showed significant difference between KKn-KK, KKn-KP1, KKn-KP2, KKn-KP3, KK-KP1, KK-KP2, KK-KP3, KP1-KP2, KP1-KP3, KP2-KP3 with *p = 0.000 (p < 0.05). Conclusion: Soursop leaves extract (Annona muricata L.) can prevent liver histological damage of mice (Mus musculus) induced by paracetamol.Keywords: Soursop leaves extract, paracetamol, histological damage of liver cell

Pengaruh Pemberian Ekstrak Kulit Manggis (Garcinia mangostana L.) dalam Mencegah Kerusakan Histopatologi Ginjal Tikus Putih (Rattus norvegicus) yang Diinduksi Gentamisin Rio Nanda Pratama; Ratih Puspita Febrinasari; Nur Hafidha Hikmayani
Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: The use of medicinal plants has been increasing in recent years following development of herbal industry and evidence on adverse effects of several chemical drugs, including nephrotoxic effect. Research on herbs with nephroprotective effect are needed to offer alternative therapy for drug-induced renal diseases. Mangosteen pericarp extract contains xanthone which has antiinflammation effect to prevent renal damages. This research aimed to examine the nephroprotective effect of mangosteen pericarp extract on renal structure. Methods: This research was a laboratory experiment with posttest only controlled group design. Subjects were white male Wistar-strain rats (Rattus norvegicus), aged 2-3 months and weighed 150-230 grams. Subjects were randomly divided into five treatment groups. Normal control group was treated 0.5% NaCMC, while negative control group was treated nothing. The treatment groups 1, 2 and 3 were treated with extract of mangosteen pericarp at doses of 62.5 mg/kg-BW, 125 mg/kg-BW, and 250 mg/kg-BW, respectively. The treatments were maintained for 2 weeks. In the second week, the negative control group and the treatment groups 1, 2, and 3 were induced by gentamicin 100 mg/kg-BW. The subjects were later terminated and their kidneys were stained with hematoxicylin eosin. Histopathological renal damages were semi-quantitatively assessed by identification of pyknotic, karyorrhexic and karyolytic areas in proximal tubulus cells and were compared using one-way ANOVA test and followed by LSD test. Result: Result of one-way ANOVA test showed that proximal tubulus cellular damage differed significantly across five groups (p < 0.001). LSD tests further showed a significant difference between negative control group and other groups (p < 0.001). Renal damage in normal control group also differed significantly from those of treatment group 1 and 3 (p < 0.001). No significant difference was found between normal control group and treatment group 2 (p = 0.972). Conclusion: Mangosteen (Garcinia mangostana L.) pericarp extract could prevent histopathological renal damage in white rats (Rattus norvegicus) induced gentamicin. Keywords: Mangosteen pericarp extract, Renal histopathology, Gentamicin.

Screening The Effectiveness of Menirans Leaf Extract (Phyllanthus niruri L.), Pepaya (Carica papaya L.), and Kunyit (Curcuma longa L.) as Antidengue In Vitro Hutami Sri Ummiyati; Ratih Puspita Febrinasari; Leli Saptawati
Nexus Biomedika Vol 5, No 1 (2016): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.864 KB)

Introduction: Dengue Haemorrhagic Fever (DHF) is an infectious disease that is caused by dengue virus. There were 71.668 DHF cases with 641 mortalities in Indonesia which was recorded from early January to mid-December 2014. Recently, DHF doesnt have a specific therapy. The aim of this study was screening about natural extracts in Indonesia as antidengue. Methods: This study was a laboratory experimental research with dengue virus serotype 2 strain New guinea C (DENV2 NGC) as a subject. This virus was infected into Huh-7 cell line. The study was conducted in 2 parts. First, dengue virus solution was mixed with natural extract and then this solution was infected to Huh-7 cell monolayer to obtain percent infectivity by FFU assay. Second,the natural extract was exposed to Huh-7 cell monolayer to obtain percent viability by MTT assay. The effective extract was selected if the extract had average percent infectivity 20% and average percent viability > 50%. Results: The percentage of viability of menirans leaf extract (Phyllanthus niruri L.), papaya (Carica papaya L.), and turmeric (Curcuma longa L.) were 96.2%, 97.5%, and 88.7% respectively. While their average percentage of infectivity were 63.9%, 10.5%, and 53.6%. Conclusion: The papayas leaf extract (Carica papaya L.) was effective as antidengue in vitro. Keywords: DENV2 NGC,dengue, meniran, papayas leaf, turmeric

Effect of EthanolicExtract of Centella asiatica againts Adrenal Gland CortexCell Lipid Droplet Feature Induced by Chronic Immobilization Stress in Rats Mochammad Fairuz Zahran; Brian Wasita; Nanang Wiyono; Ratih Puspita Febrinasari
Nexus Kedokteran Translasional Vol 4, No 1 (2015): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Chronic stress can increase the lipid droplet in cortex cell and adrenal gland damage. Pegagan extract contains such as asiaticoside, flavonoids and triterpenoid are known have antioxidant effect. The objective of the present study was to investigate the beneficial effects of Ethanolic Extract of Centella asiatica (EEC) on adrenal gland cortexcell lipid droplet feature induced by immobilization stress in male rats. Method: This study was a laboratory experimental study with randomized post test only control group design. Paraffin blocks from thirty five male rats previously subjected with chronic immobilization stress and then divided into 6 groups: control group given distilled water 1 mg/kg, stress + PGA, stress + 150 mg/kg EEC, stress + 300 mg/kg EEC, stress + 600 mg/kg EEC and stress + fluoxetine 10 mg. The treatments were performed for 21 consecutive days. Lipid droplet was evaluated using Scoring Lipid Content from Hematoxylin Eosin staining. Statistic analysis was performed using Kruskall-Wallis test continued by Post Hoc Mann-Whitney Test with Bonferroni correction. Result: Results of Kruskall Wallis test showed significant differences in six groups with p = 0.000. Mann Whitney test with Bonferroni correction showed a significant difference between control group with stress + PGA and stress + 600 mg/kg EEC. Fluexetine as positive control show the most effective dose in this study (p = 0,000). There were no significant difference between fluoxetine as control positive and control group (p = 0,112). Conclusion: Stress + 600 mg/kg EEC has beneficial effect on preventing adrenal gland cortexcell lipid droplet feature induced by chronic immobilization stress in rat. Keywords : Centella asiatica, chronic immobilization stress, adrenal glands lipid droplet feature

Perbedaan Efek Ekstrak Etanol Stevia Dibandingkan Madu Terhadap Perubahan Glukosa Darah Tikus Wistar Model Diabetik Siti Fatimah R; Kisrini .; Ratih Puspita Febrinasari
Nexus Kedokteran Translasional Vol 1, No 1 (2012): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background:Stevias leaf contains steviol, stevioside and rebaudioside which have effect to increase secretion insulin. Honey, natural sweetener contains several antioxidant such as propolis, flavonoid and vitamin. It will eliminate free radicals and indirectly improve pankreas glands cells to produce insulin. This research was aimed to know the difference effect of stevias ethanol extract comparedto honey on changing of blood glucoselevel on diabetic model Wistar rats. Methods:This was experimental research with pre and post test controlled group design. Samples were 32 male rats, Wistar Strain, 6-8 weeks and + 200 gweight. Sampling technique used in this research was incidental sampling. Samples were divided into 4 groups: negative control group (aquadest), positive control (glibenclamide dose 0.09 mg/200 g body weight), treatment group 1 (stevias ethanol extract dose 20 mg/200 g body weight), treatment group 2 (honey dose 2 ml/200 g body weight). Data were analyze using Saphiro-Wilks test of normality and Friedmann test (? = 0.05) and Post Hoc Multiple Comparison (Wilcoxon) (? = 0.05) using SPSS 17 for Windows. Results:There was a significant difference (p = 0.002) on the Wistars blood glucose level which was given stevias ethanol extract or honey. Stevias ethanol extract effect more on decreasing the blood glucose than honey. But, the both of them are able to decrease the Wistars blood glucose on diabetic model Conclusions:Stevias ethanol extract has the effect of decreasing blood glucose level on diabetic model Wistar rats better than honey. Keywords:ethanol extract of stevia, honey, alloxan, changes in blood glucose levels.

The Effect of Soybean Powder (Glycine max) to the Histological Structure of Mices (Mus musculus) Liver Cells Induced by Used Cooking Oil Selvia Anggraeni; Endang Listyaningsih Suparyanti; Ratih Puspita Febrinasari
Nexus Kedokteran Translasional Vol 3, No 1 (2014): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.207 KB)

Background: Soybean contain antioxidants which have hepatoprotective effect; one of them is isoflavone. Soybean powder has the highest isoflavone among soybean products. This research aims to prove that the use of soybean powder can prevent damage of the histological structure of the mices liver cells induced by used cooking oil. Methods: This study was an experimental laboratory with a post-test only control group design. Twenty eight Swiss Webster male mice were divided into 4 groups. Negative control group (KN) was given distilled water, the positive control (KP) was given 0.06 ml/ 20 g BW used cooking oil, dose 1 (KD1) was given soybean powder 5.6 mg/ 20 g BW and used cooking oil, and dose 2 (KD2) was given soybean powder 11.2 mg/ 20 g BW and used cooking oil. Soybean powder was given for 14 days, while the used cooking oil was given orally on 8th-14th day. On the 15th day, mice were sacrificed, taken their liver, and made ??preparations with HE staining. Liver cell damage was observed by counting the number of cells with pyknosis, karyorheksis, and karyolysis of 100 cells in liver centrolobular zone. Data were analyzed by statistical tests One-way ANOVA (Analysis of Variant) (? = 0.05). Results: The highest number of liver cells damage is on KP, followed by KD1, KD2, and at least on KN. Results of One-way ANOVA test showed significant differences among the four groups with p = 0.000 (p < 0.05). Results of Post Hoc Test of Multiple Comparasions using LSD test also showed significant differences between KN-KP, KN-KD1, KD2-KN, KP-KD1, KD2-KP, KD1-KD2 with respectively p = 0.000 (p < 0, 05). Conclusion: Soybean powder can prevent damage of the histological structure of the mices liver cells induced by used cooking oil. Keywords: soybean powder, used cooking oil, liver histological structure

Increasing Serum Transaminase and The Relation with The Serum Concentration of Isoniazid and Rifampicin of Tuberculosis Patients which Given Antituberculosis Fixed Dose Combination in Yogyakarta Ratih Puspita Febrinasari; Erna Kristin; Iwan Dwiprahasto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 47, No 4 (2015)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

ABSTRACT Tuberculosis is still a major health problem in Indonesia. Isoniazid and rifampicin are major antituberculosis drugs. These two drugs have been known to cause hepatotoxicity which is characterized by an increase of serum transaminase concentration. Previous study proved the increase of hepatotoxicity due to isoniazid and rifampicin but did not explain about the correlation with the drugs concentration. The aim of this study is to know the relation between increasing serum transaminase and the serum concentration of isoniazid and rifampicin of tuberculosis patients which given antituberculosis fixed dose combination in Yogyakarta. We carried out a cross sectional study involving 31 TB patients who received antituberculosis fixed dose combination containing isoniazid and rifampicin. Serum transaminase was measured with an automatic chemical analyzer. Isoniazid and rifampicin concentration was measured by using HPLC. We used t- test, Mann Whitney, Fisher and Spearman to analyze the data. There is no correlation between mean isoniazid concentration with high (7,07±6,24mg/ml) and normal ALT (2,42±0,26mg/ml) (p>0,05). There is no correlation between mean isoniazid concentration with high (5,03+4,14 mg/ml) and normal AST (p>0,05). There is no correlation between mean rifampicin concentration with high (10,67+3,76 mg/ml) and normal ALT (3,66+0,30 mg/ml) (p>0,05). There is no correlation between mean rifampicin concentration with high (8,52+3,06 mg/ml) and normal AST (3,64+0,32 mg/ml) (p>0,05). Mean of rifampicin concentration in this study are low (4, 12+0,46 mg/ml). So the conclusion that there are no correlation between isoniazid serum concentration with an increase of serum transaminase and no correlation between rifampicin serum concentration with an increase of serum transaminase.

BURNOUT DAN KUALITAS HIDUP CIVITAS AKADEMIKA PADA MASA PANDEMI COVID-19: STUDI DI UNIVERSITAS SEBELAS MARET Yusuf Ari Mashuri; Widana Primaningtyas; Heni Hastuti; Lukman Aryoseto; Novelia Qothrunnada; Ratih Puspita Febrinasari; Vitri Widyaningsih; Ari Natalia Probandari
Biomedika Vol 14, No 1 (2022): Biomedika Februari 2022
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v14i1.13280

ABSTRAKBurnout sering dikaitkan dengan penurunan prestasi kerja maupun belajar, bahkan mempengaruhi kehidupan keseharian individu. Sejak bulan Maret 2020 Indonesia terkena efek pandemi coronavirus disease 2019 (COVID-19), tak terkecuali pada seluruh civitas akademika Universitas Sebelas Maret (UNS). Penelitian ini bertujuan untuk mengetahui hubungan burnout dengan kualitas hidup pada civitas akademika UNS pada masa pandemi COVID-19. Penelitian ini merupakan penelitian observasional dengan pendekatan potong lintang. Survei online tentang burnout dan kualitas hidup dilakukan pada bulan Agustus – September 2020. Burnout diukur dengan skala Maslach Burnout Inventory (MBI) yang berisi 22 item pertanyaaan dan telah diadaptasi sesuai dengan konteks Indonesia. Instrumen EQ5D, berisikan 5 item pertanyaaan, yang telah diadaptasi digunakan untuk mengukur kualitas hidup responden. Terdapat hasil korelasi yang signifikan antara skor burnout dengan kualitas hidup pada dosen dan mahasiswa (r= -0,2843 dengan nilai p= 0,011dan r= -0,4045 dengan nilai p= 0,001). Sedangkan untuk tenaga kependidikan didapatkan hasil yang tidak signifikan dengan r= -0,3323 dan p= 0,0903. Usaha komprehensif untuk mengurangi burnout pada civitas akademika diperlukan sebagai upaya peningkatan kualitas hidup.Kata Kunci: Burnout, Komunitas Akademik, COVID-19, Quality of life ABSTRACTBurnout is often associated with decrease of work and study performance and could affect the daily living. Since March 2020, Indonesia has been affected by the 2019 coronavirus disease (COVID-19) pandemic, including the Universitas Sebelas Maret (UNS) academic community. This study aimed to determine the relationship between burnout and quality of life in UNS academic community during the COVID-19 pandemic. This was an observational study with a cross sectional approach. An online survey on burnout and quality of life was conducted in August - September 2020. Burnout was measured by the Maslach Burnout Inventory (MBI) scale which contains 22 question items and has been adapted according to the Indonesian context. The EQ5D instrument, which contains 5 question items, has been adapted to measure the quality of life of the respondent. There were significant correlations between the burnout score and the quality of life for the lecturers and students (r = -0.2843 with p-value = 0.011 and r = -0.4045 with p-value 0.001). Meanwhile, there was no significant result in educational staff group (r = -0.3323 with p-value = 0.0903). There were significant correlations between burnout score and quality of life for lecturers and students, but there was no significant correlation between burnout score and quality of life for education personnel. Comprehensive action to prevent burnout in academic community is needed to improve quality of life. Keywords: Burnout, Academic Community, COVID-19, Quality of life

Co-Authors . Suyatmi . Suyatmi . Widardo ., Hudiyono ., Muthmainah ., Suyatmi A.A. Ketut Agung Cahyawan W Adi Magna Patriadi Nuhriawangsa AGYA GHILMAN FAZA Ahmad Ahid Mudayana Ahmad Ahid Mudayana Akhmad Azmiardi Alfi Yasmina Ali, Agni Shalha Amer Abdulwahab Al-aghbari, Nuha Amira, Nadya Kemala Anggraeni, Selvia Anis Nurhidayati Anshari, Shafira Yasmine Argyo Demartoto, Argyo Ari Natalia Probandari Ari Probandari Arsita Eka Prasetyawati Arsita Eka Prasetyawati Asaduddin, Aiman Hilmi Asih Anggraini Asih Anggraini Astrida Fesky Febrianty Astrika Yunita, Fresthy Bachnas, Muhammad Adrianes Berlian Permata Sakti Bhisma Murti Binti Maulina Binti Maulina Putri Brian Wasita Brian Wasita Brian Wasita Bustami, Mursyid Chintya Wulandarie Colti Sistiarani Colti Sistiarani Dara, Rizki Wulan Dedi Febriandaru Desy Puspa Putri Devi Ratna Mayasari DIAN LESTARI Didik Gunawan Tamtomo Dilma’aarij, Dilma’aarij Dyonisa Nasirochmi Pakha Endang Listyaningsih Suparyanti Erna Kristin Ernawati, Dwi Arini Eti Poncorini Pamungkasari Fairuz Zamani FAZA, AGYA GHILMAN Hamka, Dwi Wulandari Aaas Hanif Mustikasari Harioputro, Dhani Redhono Hariyanti, Lusiana Pradana Hartono Hartono Haruni, Diah Sekar Heni Hastuti Hudiyono . Hutami Sri Ummiyati Ida Nurwati Ignatius Gonggo Prihatmono Imanisa, Octavia Intan Indarta, Ardhia Fefrine Irma Prasetyowati iwan Dwiprahasto Kenneth Tan Khoiriyah, A'maliatul Kisrini . Komang Windayani Kristiani, Shinta Laksmi Wardhani, Tri Martya Ningrum Leli Saptawati Lilik Wijayanti Lilik Wijayanti Lilik Wijayanti Wijayanti Lisyawati, Shanti Lukman Aryoseto Ma'rifatul Khoiriyah Ayda Maryati, Warsi Mashuri, Yusuf Ari Meintansari Manik MIFTAKHUL JANNAH Mochammad Fairuz Zahran Muchsin Doewes Mustara, Mustara Mustiqa Febriniata MUTHMAINAH . Muthmainah Muthmainah Nadya Kemala Amira Nafi'ah, Radifatun Naila Shofwati Putri Nanang Wiyono Nila Authoria Authoria Nita Dwi Astikasari Novelia Qothrunnada Novika, Revi Gama Hatta Nur Hafidha Hikmayani Nur Hafidha Hikmayani Nur Hasanah, Laras Eka Nur Hidayah Nurhasan Agung Prabowo Nurholis Majid Oktaviani oktaviani Paramasari Dirgahayu Paramasari Dirgahayu Poncorini Pamungkasari, Eti Primaningtyas, Widana Putri, Naila Shofwati Rahardjo, Setyo Sri Rio Nanda Pratama Rio Nanda Pratama Riris Arizka Wahyu Kumala Riris Arizka Wahyu Kumala, Riris Arizka Wahyu Rizaldy Pinzon RR. Ella Evrita Hestiandari Sakti, Berlian Permata Saptawati, Leli Satwika, Ariq Ratya Selvia Anggraeni Setyani, Rizka Ayu Setyani, Rizka Ayu Setyo Sri Rahardjo Setyo Sri Rahardjo Shinta Kristianti Shinta Retno Wulandari Shinta Shinta Sigit Setyawan Siswahyudianto Siti Fatimah R Solikah, Tri Agusti Sopianti, Densi Selpia Sri Darnoto Sri Mulyani Sri Mulyani Sugiarto Sumandjar, Tatar Sumardiyono Sumardiyono Sumardiyono Sumardiyono Suparyanti, Endang Listyaningsih Suparyanti, Endang Listyaningsih Susanto, Agnes Suyatmi . Syamsiar, Syamsiar Tri Agusti Sholikah Uki Retno Budihastuti Ummiyati, Hutami Sri Veronika Ika Budiastuti Vitri Widyaningsih Vitri Widyaningsih Wahyu Anjas Sari Wasita, Brian Widardo . Windayani, Komang Wiyono, Nanang Wulandari, Shinta Retno Yanuarini, Triatmi Andri Yulia Lanti Retno Dewi Yulia Sari

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