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Laili Fitri Yeni
Pendidikan Biologi FKIP Universitas Tanjungpura
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences Other

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Journal : Pharmascience

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Uji Cemaran Mikroorganisme Pada Tiga Varian Daun Kratom (Mitragyna speciosa Korth.) Widian Sari, Margareta Lita; Masriani, Masriani; Warsidah, Warsidah; Yeni, Laili Fitri; Rasmawan, Rahmat
Jurnal Pharmascience Vol 11, No 1 (2024): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v11i1.16230

Abstract

Tanaman kratom (Mitragyna speciosa Korth.) merupakan salah satu komoditas ekspor yang cukup menjanjikan dari Indonesia. Salah satu syarat suatu komoditas dapat diekspor harus memenuhi batasan kandungan mikrobiologi yang mengacu pada SNI dan AHPA. Bahan baku kratom yang terkontaminasi mikroorganisme, terutama mikroorganisme patogen akan membahayakan kesehatan konsumen. Oleh karena itu, penelitian ini bertujuan untuk menganalisis cemaran mikrobiologis tiga varian daun kratom, yaitu kratom merah, putih, dan hijau dalam bentuk serbuk kering simplisia. Uji cemaran mikrobiologi daun kratom meliputi cemaran bakteri Salmonella sp., Escherichia coli, dan kapang khamir. Uji cemaran bakteri Salmonella sp. dilakukan dengan menggunakan media Xylose Lysine Deoxycholate (XLD) dan Brilliat Green Agar (BGA) dengan suhu inkubasi 37oC selama 24 jam. Uji cemaran terhadap bakteri E. coli dilakukan dengan menggunakan media ColiformEscherichia coli Color Indicator dengan suhu inkubasi 35oC selama 24 jam. Penentuan kontaminasi kapang khamir menggunakan media Yeast and Mold Color Indicator dengan suhu 25oC selama 72 jam. Hasil uji yang telah dilakukan menunjukkan bahwa daun kratom merah, putih dan hijau tidak terkontaminasi bakteri Salmonella sp. dan E. Colitetapi terkontaminasi kapang khamir  yang lebih dari 104 koloni/gram. Kata Kunci: Ganja Borneo, Salmonella sp., Escherichia coli, Kapang Khamir, Koloni  The kratom plant (Mitragyna speciosa Korth.) is one of the most promising export commodities from Indonesia. One of the conditions for a commodity to be exported must meet the microbiological content limits referring to SNI and AHPA. Export commodities that do no meet the standards will be detained during the production and delivery process. Therefore, this study aims to analyze the microbiological contamination of three variants of kratom leaves, namely red, white and green kratom. Tests for microbiological contamination of kratom leaves include contamination of Salmonella sp., Escherichia coli, and yeast molds. Salmonella sp. bacteria contamination test carried out using Xylose Lysine Deoxycholate (XLD) and Brilliat Green Agar (BGA) media with an incubation temperature of 37oC for 24 hours. Contamination tests on E. coli bacteria were carried out using Coliform Escherichia coli Color Indicator media with an incubation temperature of 35oC for 24 hours. Determination of yeast contamination using Yeast and Mold Color Indicator media at 25oC for 72 hours. The results of the tests that have been carried out show that the red, white and green kratom leaves are not contaminated with Salmonella sp. and E. coli, but contaminated with yeast and Mold which is more than 104 colonies/gram.
Co-Authors ,, Khoirunnisa ,, Yansah A'yun, Tiara Nissa Ade Mirza Adi Hidayat Afandi Afandi Ahmad Aloy Saputra Amelia Purwaningsih Andarwatiningrum, Nurul Andi Besse Tenriawaru Andini, Dini Tiara Anduweni Anggi Kalista Anisyah Yuniarti Anisyah Yuniarti Anjeliana, Anjeliana Annisa Nabilah Mufidah Aprianty, Vetty Aprilianti, Melda Ardianti, Dwi Ariswandi, Ariswandi Aryanto Aryanto Aso, Merlinda Ugha Asriah Nurdini M. Bonifasius, Bonifasius Chaerani Chaerani Chaerani Cici Yulianti Damai Yanti Dewi Kurnializa Diana Fransiska Dinda Triana Dinda Triana Dwitia, Widi Ek Ariyati Eka Ariyati Eka Irvianti Pratiwi Eka Mardianti Eka Sari Eka Sari, Eka Eko Sri Wahyuni Eko Sri Wahyuni Eko Sri Wahyuni Eko Sri Wahyuni Elisa Agustini Elsa Putri Emmi, Natalia Eni Eka Sari Entin Daningsih Erma Erma Erma Fajri, Hayatul Febriani, Sherly Fitri, Jumrotul Fitriyana Fitriyana, Fitriyana Handayani, Deasy Rizki Hardigalu, Basuki Hendry, Yerianus Hening Prihatin Ningrum Herkulanus Hendi Daniel Herra Wastu Widanti Hidayah, Jujur Ridha Icha Monika Iin, Yesi Ika Kartika Ira Lestari Isyatirradhiyah, Isyatirradhiyah Jayanti, Wahyu Ratna Junika Mandasari Junika Mandasari Kesumawati, Inggit Khairi Khairi Kumalasari, Muthia Kurnia Ningsih Kurniawan, Rachmat Marta Maria Dona Mas Akhbar Faturrahman Masriani . Meilisa Ella Putri Miranti . Mufidah, Annisa Nabilah Muhammad , Muhammad Syukur Ningsih, Kurnia Ningsih, Kurnia Ningsih NINGSIH, NINGSIH Nurfatehah Nurlaili Nurlaili Nursamsiyah, Nursamsiyah Nury Kamelia Nury Kamelia Oktaviani, Dwi Pasaribu, Revi Lestari Prastiwi, Sherin Widya Purwaningsih, Amelia Putri, Elsa Putri Putri, Ruqiah Ganda Rabeka, Rabeka Rahmat Rasmawan Rahmayanti, Sofia Rara Sania Riki, Ajustus Rini Muharini Riska Maulidya Risma Butar Butar, Risma Rizki Utami Romika Romika RR. Ella Evrita Hestiandari Ruqiah Ganda Putri Panjaitan Sania, Rara Saptiansyah Syafrizal Saputri, Emilta Sari, Eni Eka Sari, Hertika Sayyidah Asmah Sellina Yessi Wulandari Selvira Septiyanda Septiani, Fransiska Sherly Febriani Siti Fauziah Suprehatin ,, Suprehatin Syakuran, Luqman Abdan Syam swisna Syamswisna , T. Adri Abi Tenriawaru, Andi Besse Tiara Nissa A'yun Titin . Titin Titin Titin Titin Umi Kalsum Uray Rina Septiani Wahyu Ratna Jayanti Wahyu, Eko Sri Wahyuni, Eko Sri Wahyuni Warsidah, Warsidah Widi Dwitia Widian Sari, Margareta Lita widya astuti Widya Ningsih Widyastuti, Desinta Ayu Winda Astuti WOLLY CANDRAMILA Wulansari, Tia Yohanes Andri Yokhebed . Yokhebed . Yokhebed Yokhebed Yudi Kurniawan