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All Journal Jurnal Sain Veteriner SAINS DAN MATEMATIKA Prosiding Seminar Biologi Health Science Journal of Indonesia Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Urology agriTECH Indonesian Journal of Biotechnology INDONESIAN JOURNAL OF PHARMACY BERITA BIOLOGI Dental Journal (Majalah Kedokteran Gigi) The Indonesian Biomedical Journal Universa Medicina Medical Laboratory Technology Journal Molecular and Cellular Biomedical Sciences (MCBS) Molekul: Jurnal Ilmiah Kimia Oto Rhino Laryngologica Indonesiana INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Journal of Herbal, Clinical and Pharmaceutical Science (HERCLIPS) Narra J Proceeding Biology Education Conference Cermin Dunia Kedokteran Nutrisia Medical and Health Journal Jurnal Pandu Husada
Sofia Mubarika Haryana
Departement Of Histology And Biology Cell, Faculty Of Medicine, Public Health And Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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Journal : INDONESIAN JOURNAL OF PHARMACY

 1 
Phalerin, a new benzophenoic glucoside isolated from the methanolic extract of Mahkota Dewa [Phaleria macrocarpa (scheff). Boerl.] leaves Hartati W, Mae Sri; Mubarika, Sofia; Gandjar, Ibnu G; Hamann, Mark T; Rao, KV; Wahyuono, Subagus
INDONESIAN JOURNAL OF PHARMACY Vol 16 No 1, 2005
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.086 KB) | DOI: 10.14499/indonesianjpharm0iss0pp51-57

Abstract

Mahkota Dewa [Phaleria macrocarpa (Scheff). Boerl.] is used traditionally to treat cancers in Indonesia. Extract methanol of the leaves displayed a small LC50 value (63.16 μg/ml) on BST (Brine Shrimp Lethality test) assay; therefore a phytochemical study of this extract was undertaken. A new benzophenoic glucoside was isolated from the methanol extract and identified as 4,5-dihydroxy,4’-methoxybenzophenone-3-0-β-D-glucoside (Phalerin) based on its spectroscopic data. Phalerin was cytotoxic to myeloma cell line (NS-1) (in vitro) having IC50 of 83 μg/ml or 1.9 x 10-1 mM.Key words: Phaleria macrocarpa, Phalerin, myeloma
Structure identification of potential compound as selective renal anticancer isolated from Nerium indicum Mill. Leaves Mae S.H. Wahyuningsih; Sofia Mubarika; Mark T. Hamann; Ibnu G. Gandjar; Subagus Wahyuono
Indonesian Journal of Pharmacy Vol 19 No 2, 2008
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (850.839 KB) | DOI: 10.14499/indonesianjpharm0iss0pp57-64

Abstract

Oleandrin is one of cardenolida compound isolated from an active fraction of Nerium indicum Mill leaves. (fam. Apocynaceae), which have cytotoxic effect on several human cancer cells, and also to normal cells in vitro. Another compound which was potential as renal anticancer has also been isolated from N. indicum. However, its chemical structure has not been discovered. The aim of this study was to identify the potential compound as selective to renal cancer present in the leaves of N. indicum.The potential compound was isolated from the active fraction using Preparative TLC and structure elucidation was done by using spectroscopic methods (UV, IR, MS and NMR).Base on this spectra and by comparison with oleandrin data, it was indicated that the potential compound as Renal anticancer was as 16,17-dehydrodeacetyl-5a-oleandrin.Key word: N.indicum, oleandrin, cytotoxic, renal anticancer, spectroscopic
PROTECTIVE EFFECT ETHANOLIC EXTRACT OF Boesenbergia pandurata (ROXB.) Schlecht. AGAINST UVB-INDUCED DNA DAMAGES IN BALB/C MICE Shanti Listyawati; Sismindari Sismindari; Sofia Mubarika; Yosi Bayu Murti
Indonesian Journal of Pharmacy Vol 26 No 2, 2015
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (751.14 KB) | DOI: 10.14499/indonesianjpharm26iss2pp108

Abstract

Boesenbergia pandurata (Roxb.) Schlecht. contains bioactive compounds that have a number healthy effect including anti-oxidant and anti-carcinogenic activity. This research was carried out to examine the protective effect of B. pandurata extract against expression of cyclobutane pyrimidine dimers (CPDs) as marker of UVB-induced DNA damage in Balb/c mice. Dried powder of B. pandurata rhizomes was extracted by maceration method using 96% ethanol. The extract was quantified with pinostrobin as active marker using TLC scanner. Ethanolic extract of B. pandurata (EEBP) was given orally at 14 days before UV exposure with a variety doses, 0 (vehicle), 20, 40 and 60mg/kgBW/day and continuing until termination of the experiment. Following the UVB irradiation (1.4J/m2), mice were sacrificed at different time points (2, 24, 48, and 72h after UVB exposure). The back skin samples were collected to analyze CPDs expression by immunohistochemical method. The result showed that EEBP (contained 5% pinostrobin) dose was 40 and 60mg/kgBW/day had protective activity against UV-induced DNA damage as indicated by the decrease of CPDs expression.   Key words:  Boesenbergia pandurata (Roxb.) Schlecht., UVB, DNA damage, CPDs.
SECONDARY BIOACTIVE METABOLITE GENE CLUSTERS IDENTIFICATION OF ANTICANDIDA-PRODUCING Streptomyces Sp. GMR22 ISOLATED FROM WANAGAMA FOREST AS REVEALED BY GENOME MINING APPROACH Camelia Herdini; Sofia Mubarika; Bambang Hariwiyanto; Nastiti Wijayanti; Akira Hosoyama; Atsushi Yamazoe; Hideaki Nojiri; Jaka Widada
Indonesian Journal of Pharmacy Vol 28 No 1, 2017
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1110.637 KB) | DOI: 10.14499/indonesianjpharm28iss1pp26

Abstract

Streptomyces are a group of Gram-positive bacteria belonging to the Actinobacteria class, which are among the most important bacteria for producing secondary bioactive metabolites such as antibiotics, chemotherapeutics, insecticides and other high-value chemicals. Genome mining of gene clusters that encode the biosynthetic pathways for these metabolites has become a key methodology for novel compound discovery. Recently, we have isolated the Streptomyces sp. GMR22 from Cajuput rhizospheric soil at Wanagama Forest, Indonesia. GMR22 produced secondary metabolite that inhibited Candida albicans with IC50 of 62,5 μg/mL. The objective of this work was to reveal the novel secondary metabolites from GMR22 by genome mining approach. The antiSMASH 3.0 was used to predict gene clusters that encode the biosynthetic pathways of secondary metabolites in the genome of GMR22, and their core chemical structures. The pylogenomic analysis showed that GMR22 was closely related to Streptomyces bingchenggensis BCW1, as well as to the large genome size (9.5-12.7Mbp) groups of Streptomyces. AntiSMASH 3.0 analysis revealed that the genome of Streptomyces sp. GMR22 harbored at least 63 gene clusters that encode the biosynthetic pathways of secondary metabolites. It was the highest number of gene clusters had been observed among the members of Streptomyces groups, with PKS was predicted as the major groups of the identified gene cluster products. The results suggested that GMR22 could be a strong potential candidate of secondary bioactive metabolites source.
Cytotoxicity of oleandrin isolated from the leaves of Nerium indicum Mill. on several human cancer cell lines Mae S.H. Wahyuningsih; Sofia Mubarika; R.L.M. Bolhuis; K. Nooter; Ibnu G. Gandjar; Subagus Wahyuono
Indonesian Journal of Pharmacy Vol 15 No 2, 2004
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.917 KB) | DOI: 10.14499/indonesianjpharm0iss0pp96-103

Abstract

Finding anticancer drugs from natural resources still proceeds. Oleandrin isolated from Nerium indicum Mill. inhibited the growth of mieloma cell line in vitro better than that of vincristine sulphate. This study was aimed to determine the cytotoxic effect of oleandrin on various human cancer cell lines. Cytotoxic test of oleandrin on seven human cancer cell lines was done by SRB-method. The analysis was conducted by comparing the ID50 of oleandrin with that of doxorubicin and cisplatin as positive controls. This result indicated that oleandrin possessed the best cytotoxic effect on breast cancer (MCF7) with ID50 at 8.85 nM. Keywords : Oleandrin, cytotoxicity, human cancer cells, ID50
Detection of apoptosis mechanism on renal cancer cell treated by 16,17-dehydrodeacetyl-5α-oleandrin compound isolated from Nerium indicum Mill. Leaves. Mae S.H. Wahyuningnsih; Sofia Mubarika; Ibnu G. Gandjar; Subagus Wahyuono; AWM. Boersma; K. Nooter
Indonesian Journal of Pharmacy Vol 19 No 4, 2008
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (557.149 KB) | DOI: 10.14499/indonesianjpharm0iss0pp178-184

Abstract

The 16,17-dehydrodeacetyl-5α-oleandrin was isolated from an active fraction of Nerium indicum Mill leaves (fam. Apocynaceae). This compound was cytotoxic against various cancer cells, and selective on A498 cells (Renal cancer). However, the apoptosis mechanism was still unknown yet. Therefore, the aim of this study was to know the apoptotic mechanism of 16,17-dehydrodeacetyl-5α-oleandrin on A498 cells by FITC labeled annexin V and immunucytochemical assays. The detection of apoptotic mechanism on A498 cells was performed with FITC-conjugated annexin V using Flow Cytometry. The p53 protein expression were detected using immunocytochemical. Treatment with 16,17-hydrodeacetyl-5α-oleandrin (3.88 x 10-4 mM) using FITC-annexin V increased the percentage of the dead cells in the 24th and 48th hours incubation period. The 16,17-dehydrodeacetyl-5α-oleandrin (1,94x10-4 and 3,88x10-4 mM) raised significanly p53 protein expression (p<0,05). The percentage of the p53 protein expression increased throughout the time of samples incubation.Key words: 16,17-dehydrodeacetyl-5α-oleandrin,fluorescein isothiocyanate-annexin V, immunocytochemically, A498 cells.
Co-Authors . Irianianiwati . Suharno Abdurahman Laqif Abdurahman Laqif Addin Trirahmanto Agnes Murdiat Agnes Murdiati Agus Surono Ahmad Ghozali Ahmad Ghozali Ahmad Hamim Sadewa Akbar Satria Fitriawan Akira Hosoyama Akira Hosoyama Alfasunu, Serafim Aminuyati Anwar, Sumadi Lukman Aprilia Indra Kartika Aprilia Indra Kartika Aris Haryanto Aris Haryanto Arsi Palupi Atsushi Yamazoe Atsushi Yamazoe AWM. Boersma Bambang Hariwiyanto Bambang Hariwiyanto Bambang Hariwiyanto Bernadia Branitamahisi Bernadia Branitamahisi Bolhuis RLH Camelia Herdini Christiana Tri Nuryana Christina Hari Nawangsih Priharsanti Christina Prihharsanti Cita Herawati Daan Khambri Damiana Sapta Candrasari Danarto Danarto Danarto Danarto Danarto Danarto Demas Bayu Handika Dessy Arisanty Dewi Agustina Dewi Sahfitri Tanjung Dewi Sahfitri Tanjung Diah Rumekti Hadiati Dibyo Pramono Didik Setyo Heiyanto Didik Setyo Heriyanto Dinna Rakhmina Dwi Aris Agung Nugrahaningsih Dwi Nur Indah Sari Edy Meiyanto Eka Savitri Endang Astuti Endang Astuti Fatma Zuhrotun Nisa Fatma Zuhrotun Nisa Firly Putri Fardhila Hanafi, Arif Riswahyudi Hartopo, Anggoro B. Heru Pradjatmo Hideaki Nojiri Hideaki Nojiri Ibnu G Gandjar, Ibnu G Ibnu G. Gandjar Ibnu G. Gandjar Ibnu G. Gandjar Ibnu G. Ganjar, Ibnu G. Ibnu Purwanto Ida Ayu Preharsini Ida Ayu Preharsini Kusuma Ifrinda Giantari Imelda, Priscillia Indwiani Astuti Indwiani Astuti Indwiani Astuti Indwiani Astuti Indwiani Astuti Iqmal Tahir Irianiwati Widodo Isnatin Miladiyah Iwan Dwiprahasto Iwan Dwiprahasto Jaap Middeldorp Jajah Fachiro JAKA WIDADA Jayusman, Achmad Mulawarman Jumina Jumina Juwita Raditya K. Nooter K. Nooter Ketut Sofjan Firdausi, Ketut Sofjan KV Rao, KV M. Munir Mae S.H. Wahyuningnsih Mae Sri Hartati Wahyuningsih Mark T Hamann, Mark T Mark T. Hamann Mary Astuti Mary Astuti Maya Esther Wullur Moningka Meutia Srikandi Fitria Mohammad Hakimi Nanda Qoriansas Nastiti Wijayanti Nastiti Wijayanti Nastiti Wijayanti Neneng Ratnasari Nilasari, Fita Nooter K Nor Sri Inayati Nor Sri Inayati Novi Febrianti Nur Arfian Nur Arfian Nur Signa Gumilas Oktriani R Oostrum RG Perkasa, DP Pinandi Sri Pudyani Puji Lestari R.L.M. Bolhuis Rachma Greta Perdana Putri Rachma Greta Putri Raden Danarto Renovaldi, Dede Retno Arianingrum Retno Sunarminingsih Sudibyo Rina Triasih Risky Oktriani Ronny Martien S. Sismindari Sagung Rai Indrasari Salugu Masesadji Sari Eka Pratiwi Satriyo, Pamungkas Bagus Sa’adah N Shanti Listyawati SHANTI LISTYAWATI Shanti Listyawati Shinta Hartanto Siregar, Fajri M. Sismindari . Sismindari Sismindari Sismindari Sismindari Siti Boedina Kresno Siti Nur Chasanah Siti Nur Chasanah Soenarto Sastrowiyoto Sri Nuryani Wahyuningrum Sri Nuryani Wahyuningrum Sri Nuryani Wahyuningrum Sri Nuryani Wahyuningrum Sri Suparwitri Stefani Candra Firmanti Subagus Wahyuono Subagus Wahyuono Subagus Wahyuono Subagus Wahyuono Sukarti Moeljopawiro Sumadi, Firasti A.N Sumawan, Herman Susanna Hutajulu Tatsuo Takeya, Tatsuo Teguh Aryandono Tiara Puspita Agustin Tirta wardana Torizal GF Tri Wibawa Triana Hertiani Umar Anggara Jenie Umar Anggara Jenie Wardana T wardana, Tirta Widhiastuti, Stefani Santi Wirsma Arif Harahap Yanwirasti - Yohanes Widodo Wirohadidjojo Yosi B. Murti Yosi Bayu Murti Ysrafil .