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All Journal MEDISAINS Biology, Medicine, & Natural Product Chemistry Mutiara Medika: Jurnal Kedokteran dan Kesehatan JURNAL BIOLOGI INDONESIA Universa Medicina Jurnal Kesehatan Vokasional Jurnal Ilmu Kefarmasian Indonesia YARSI Medical Journal Sumatera Medical Journal Majalah Kesehatan Pharmamedika Medika Alkhairaat : Jurnal Penelitian Kedokteran dan Kesehatan Medicra (Journal of Medical Laboratory Science/Technology) Proceeding ISETH (International Summit on Science, Technology, and Humanity) Junior Medical Journal Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)
Restu Syamsul Hadi
UNIVERSITAS YARSI
Religion Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Education Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Journal : MEDISAINS

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Green tea leaf extract reduces viability and migration of cholesteatoma fibroblast of chronic suppurative otitis media cultured in vitro Rizki, Anika; Suciati, Yulia; Hadi, Restu Syamsul
MEDISAINS Vol 21, No 2 (2023)
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/medisains.v21i2.17049

Abstract

Background: Chronic Suppurative Otitis Media (CSOM) is still a health problem, especially in developing countries. CSOM with cholesteatoma is a dangerous type. Cholesteatoma in CSOM sufferers can cause various complications. Therefore, alternative therapies are needed, such as green tea leaf extract. Green tea leaf extract can be an antioxidant and anti-inflammatory, but its effectiveness in treating CSOM has not been studied before, so research is needed..   Purpose: This research aimed to determine the effect of green tea leaf extract that could reduce viability and migration in cholesteatoma fibroblast of CSOM.Methods: This research was an in vitro experiment with a post-test-only control group design. The sample for this research was cholesteatoma fibroblast cells obtained from the isolation of patients with CSOM. The method used is Hoechst staining for viability and scratch techniques for cell migration with eight groups of cholesteatoma fibroblasts consisting of a negative control group (DMEM+FBS), DMEM group, two positive control groups dexamethasone (10 µM and 100 µM), and four green tea leaf extract group (10 µg/ml, 40 µg/ml, 80 µg/ml, and 160 µg/ml). Data analysis used One-Way ANOVA and Kruskal Wallis tests.Results: The results showed that the highest average cell viability was in the negative control group (165.33), and the lowest was in green tea leaf extract at a dose of 160 µg/ml (70.88). Cell migration at 72 hours showed that in the negative control group, there was faster closure of the scratch area (97,78%) and the slowest closure on green tea leaf extract at a dose of 160 µg/ml (13,81%).Conclusion: Green tea leaf extract can reduce the viability and migration in cholesteatoma fibroblast of CSOM. It shows the potential of green tea extract as an alternative to prevent cholesteatoma.
In vitro study: thymoquinone inhibits the proliferation and migration of keloid fibroblasts and increases their apoptosis Hadi, Restu Syamsul; Juniarti, Juniarti
MEDISAINS Early Release
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/medisains.v0i0.23975

Abstract

Background: No treatment can eliminate keloids. Thymoquinone (TQ) is hypothesized to play a pivotal role in treating keloids by modulating cellular processes such as proliferation, migration, and apoptosis. However, the existing studies investigating its effects on these mechanisms in keloid fibroblasts are limited and require further exploration.Objective: This study aims to investigate the effects of TQ on the proliferation, migration, and apoptosis of keloid fibroblasts in vitro.Methods: This experimental study was conducted using keloid fibroblast cultured in vitro. Cells were seeded in a 96-well plate at a density of about 5x103 cells per well with 100 μl of culture medium, and cells were cultured for 24, 48, and 72 hr for each concentration of TQ. Cell proliferation was assessed using a CCK-8 Kit, measuring optical density with a microplate reader. Apoptosis was measured using the TUNEL assay. Cell migration following TQ treatment was evaluated using the Scratch assay. The statistical test used a one-way analysis of variance (ANOVA) followed by the least significant difference (LSD) test. Results: TQ inhibited the proliferation of keloid fibroblasts at a dose of 5 µM after 48 hours of incubation and 10 µM after 24 hours of incubation. The inhibitory effect of TQ on fibroblast proliferation increased in a dose- and time-dependent manner. Treatment at 5 and 10 µM doses increased apoptosis in keloid fibroblast cultures. The TQ5 µM group achieved 60% closure, while the 10 µM group showed 55% closure. Migration was significantly inhibited in the 25 µM and 50 µM groups, with only 30% and 10% closure, respectively, at 72 hours.Conclusion: Thymoquinone inhibits the proliferation and migration of keloid fibroblast cells while promoting apoptosis. These properties suggest that TQ could be developed as a potential treatment for keloid-related skin issues.
In vitro study: thymoquinone inhibits the proliferation and migration of keloid fibroblasts and increases their apoptosis Hadi, Restu Syamsul; Juniarti, Juniarti
MEDISAINS: Jurnal Ilmiah Ilmu-Ilmu Kesehatan Early Release
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/medisains.v0i0.23975

Abstract

Background: No treatment can eliminate keloids. Thymoquinone (TQ) is hypothesized to play a pivotal role in treating keloids by modulating cellular processes such as proliferation, migration, and apoptosis. However, the existing studies investigating its effects on these mechanisms in keloid fibroblasts are limited and require further exploration.Objective: This study aims to investigate the effects of TQ on the proliferation, migration, and apoptosis of keloid fibroblasts in vitro.Methods: This experimental study was conducted using keloid fibroblast cultured in vitro. Cells were seeded in a 96-well plate at a density of about 5x103 cells per well with 100 μl of culture medium, and cells were cultured for 24, 48, and 72 hr for each concentration of TQ. Cell proliferation was assessed using a CCK-8 Kit, measuring optical density with a microplate reader. Apoptosis was measured using the TUNEL assay. Cell migration following TQ treatment was evaluated using the Scratch assay. The statistical test used a one-way analysis of variance (ANOVA) followed by the least significant difference (LSD) test. Results: TQ inhibited the proliferation of keloid fibroblasts at a dose of 5 µM after 48 hours of incubation and 10 µM after 24 hours of incubation. The inhibitory effect of TQ on fibroblast proliferation increased in a dose- and time-dependent manner. Treatment at 5 and 10 µM doses increased apoptosis in keloid fibroblast cultures. The TQ5 µM group achieved 60% closure, while the 10 µM group showed 55% closure. Migration was significantly inhibited in the 25 µM and 50 µM groups, with only 30% and 10% closure, respectively, at 72 hours.Conclusion: Thymoquinone inhibits the proliferation and migration of keloid fibroblast cells while promoting apoptosis. These properties suggest that TQ could be developed as a potential treatment for keloid-related skin issues.
Co-Authors AGUNG ERU WIBOWO Artha Budi Susila Duarsa Azmi, Sabrina CHURIYAH CHURIYAH Damayanti, Ndaru Andri Dewi, Lia Sari Utami Elmi Nuryati Elmi Nuryati FAIZA KARA FABIOLA Fasihah, Mariah Shofwah Fatimah Eliana, Fatimah Firman Arifandi Hakim, Jasir Indra Kusuma Indra Kusuma Irwandi, Nurmayani Juniarti . Juniarti Juniarti Kuslestari, K Kusumah, Indra Marsiati, Himmi Maudina Mahlani Mustofa, Muhammad Samsul Nadira, Nadira Novita Sari, Sonia Nunung Ainur Rahmah Nur Fadhilah Nur Fadhilah Parwanto, Edy Pendrianto Pendrianto, Pendrianto Pratiwi, Nike Rahmah Aprilia, Yoan Razari, Intan Reza Aditya Digambiro Rizki, Anika Rofilah, Dian Samsul Mustofa Samsul Mustofa Sembiring, Rinawati SISKA A KUSUMASTUTI Siti Marhamah, Siti Sofwan, Achmad TATI NURHAYATI Thamrin, Nabila Pyrenina TITIEK DJANNATUN Titiek Djannatun Tri Panjiasih Susmiarsih Ulviena, Lulu Wening Sari Yulia Suciati Yurika Sandra