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All Journal MEDISAINS Biology, Medicine, & Natural Product Chemistry Mutiara Medika: Jurnal Kedokteran dan Kesehatan JURNAL BIOLOGI INDONESIA Universa Medicina Jurnal Kesehatan Vokasional Jurnal Ilmu Kefarmasian Indonesia YARSI Medical Journal Jurnal Kesehatan Majalah Kesehatan Pharmamedika Medika Alkhairaat : Jurnal Penelitian Kedokteran dan Kesehatan Medicra (Journal of Medical Laboratory Science/Technology) Proceeding ISETH (International Summit on Science, Technology, and Humanity) Info Abdi Cendekia Junior Medical Journal Indonesian Journal of Multidisciplinary on Social and Technology Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)

Restu Syamsul Hadi

Fakultas Kedokteran Universitas YARSI

Author-ID : 272227

Religion Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Economics, Econometrics & Finance Education Engineering Health Professions Immunology & microbiology Library & Information Science Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Isolasi Sel Punca Pluripoten dengan Penanda CD105+ dan SSEA3+ dari Sel Fibroblas Kulit asal Jaringan Preputium CHURIYAH CHURIYAH; INDRA KUSUMA; SISKA A KUSUMASTUTI; RESTU SYAMSUL HADI; AGUNG ERU WIBOWO; FAIZA KARA FABIOLA
JURNAL ILMU KEFARMASIAN INDONESIA Vol 14 No 2 (2016): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2117.621 KB)

Perkembangan penelitian dan pengetahuan tentang sel punca terus meningkat, kini sel punca berpeluang menjadi terapi modern dengan pendekatan regeneratif yang memberi harapan kesembuhan untuk berbagai jenis penyakit yang sulit untuk disembuhkan seperti penyakit genetik, degeneratif, trauma dan keganasan.Tujuan penelitian ini adalah melakukan isolasi sel punca pluripoten dari sel fibroblas kulit asal jaringan preputium secara enzimatis dan eksplan yang dilanjutkan dengan karakterisasi penanda khas cluster differentiation (CD) 105 dan surface-stage embryonic antigen (SSEA)-3 menggunakan sistem pemurnian berbasis magnet (MACS). Hasil isolasi sel fibroblast secara enzimatik maupun eksplan diperbanyak secara kultur ekspansi dengan variasi medium dan pasase sel serta dikarakterisasi untuk mendapatkan subpopulasi sel dengan CD 105+ dan SSEA3+. Penggunaan conditioned medium, tingkat pasasi yang rendah dan konfluensi yang tinggi meningkatkan persentase subpopulasi sel CD105+ dibandingkan dengan medium standar, tingkat pasase yang lebih tinggi dan konfluensi yang lebih rendah.Subpopulasi sel CD 105+ dan SSEA3+ yang dikultur dan diekspansi menampakkan respon terhadap pewarna Alkalin fosfatase yang menunjukkan adanya populasi stem cell.

Pengaruh Fermentasi Madu (Apis Mellifera) Terhadap Gambaran Histologi Ginjal Tikus yang Diinduksi Cisplatin dan Tinjauannya Menurut Pandangan Islam Elza Rakhma Octaviaini Permana; Restu Syamsul Hadi; Firman Arifandi
Junior Medical Journal Vol 1, No 2 (2022)
Publisher : Junior Medical Journal

Pendahuluan : Pengobatan kanker yang paling efektif dan yang paling sering digunakan adalah kemoterapi dengan cisplatin. Cisplatin bekerja secara sistemik, sehingga bukan hanya sel kanker saja yang dikenai, tetapi sel-sel sekitarnya yang sehat juga terkena efek sampingnya sehingga penggunaan obat ini harus dibatasi. Perlu adanya solusi yang dapat mengurangi efek samping cisplatin. Bahan alam yang dapat menurunkan efek samping cisplatin adalah fermentasi madu. Metodologi : Penelitian ini menggunakan data primer dengan rancangan eksperimental posttest onlycontrol group design secara in vivo. Penelitian ini memiliki 4 kelompok percobaan yaitu, kelompok kontrol tanpa perlakuan, kelompok cisplatin, kelompok perlakuan fermentasi madu 5% dan paparan cisplatin, serta kelompok perlakuan fermentasi madu 10% dan paparan cisplatin. Setelah itu dilakukan pewarnaan HE dan dilakukan pengamatan secara deksriptif dan kuantitaif. Hasil : Fermentasi madu dapat memberikan efek perubahan ukuran glomerulus, tubulus distal, dan tubulus proksimal mencapai normal. Simpulan : Pemberian fermentasi madu dapat bersifat nefroprotektif dari efek samping cisplatin. Dalam pandangan Islam madu adalah kebesaran Allah yang telah disebutkan di dalam Al-Quran yang dinamai dengan Surah An-Nahl (Lebah).

The Effect of Honey (Apis Mellifera) Fermentation On Cisplatin Induced Hepatic Histology of Rats and The Review According to Islamic Perspective Fakhria Mutia; Restu Syamsul Hadi; Firman Arifandi
Junior Medical Journal Vol 1, No 2 (2022)
Publisher : Junior Medical Journal

Background: Cancer is one of the main killer diseases in the world. Cancer must be treated properly. The most common cancer treatment is chemotherapy. Cisplatin is one of the chemotherapy drugs used because of its wide efficacy for the treatment of various types of cancer. Side effects of cisplatin include ototoxicity, nephrotoxicity, hepatotoxicity, neurotoxicity and bone marrow depression. Therefore, there is a need for a solution that can reduce the side effects of cisplatin. One of the natural ingredients that can reduce the side effects of cisplatin is honey. Methods: This research is in the form of primary data, the data is taken directly by conducting an in vivo experiment. This study was divided into 4 groups, namely the untreated group, the cisplatin treatment group only, the cisplatin treatment group with 5% honey fermentation and the cisplatin treatment group with 10% honey fermentation. After that, HE staining was performed to see the histopathological picture of liver cells. Results: Fermentation of honey can reduce the size of congestion and hemorrhage from liver cells exposed to cisplatin. Conclusion: Fermented honey can protect the liver from the toxic effects of cisplatin exposure. In the view of Islam, treatment is one of the efforts in getting the gift of healing from Allah Subhanahu wa Ta'ala. Islam and medicine agree on the importance of this research to support the goals of Islamic law, namely Maqashid Syari'iyah.

Konsentrasi Basic Fibroblast Growth Factor (bFGF) dan Transforming Growth Factor-Beta 1 (TGF-β1) pada Penyembuhan Luka Kulit Tikus Anggi Puspa Nur Hidayati; Wira Alwysaputro; Afif Ramdisa; Restu Syamsul Hadi
Jurnal Kesehatan Vol 11 No 1 (2023): April 2023
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/j-kes.v11i1.337

Acceleration of wound healing is now becomes a trend center for many researcher to explore various herbs which are containing compounds that can affecting some factors involved in wound healing process. Growth factors is one of the marker that can be analyzed whether a certain herb can accelerate wound healing. To better understand how a certain herb invloved in the acceleration of wound healing, a growth factors-study was conducted. Archived skin tissue of rats from previous study (unpublished) which were been treated with Anredera cordifolia (Binahong) extract were used, and subjected to enzyme-linked immunosorbent assay (ELISA) to quantify bFGF and TGF-β1. The results obtained was then analyzed descriptively between the control group (Binahong-untreated) and treatment group (Binahong-treated). The ELISA results showed no siginificant difference of bFGF and TGF-β1 concentration between control group (Binahong-untreated) and treatment group (Binahong-treated). However, the concentration of bFGF dan TGF-β1 on day 14 were decreasing compared with those concentration on day 7, assumed the wound healing phase has already entering the next phase where the production of bFGF and TGF-β1 have been replaced with other growth factors involved in wound healing process

Pengaruh Fermentasi Madu (Apis Mellifera) Terhadap Histologi Pankreas Tikus Yang Diinduksi Cisplatin dan Tinjaunnya Menurut Pandangan Islam Maudina Mahlani; Restu Syamsul Hadi; Firman Arifandi; Samsul Mustofa
Junior Medical Journal Vol 2, No 1 (2023)
Publisher : Junior Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jmj.v2i1.3806

Kanker adalah perubahan yang abnormal dalam perkembangan sel yang berkembang menjadi keganasan dalam jaringan manusia. Sel-sel ini berkapasitas untuk memperbanyak diri serta menyebar ke berbagai bagian tubuh yang berakibat mematikan. Cisplatin sebagai terapi kanker juga memiliki efek samping pada jaringan pankreas. Sehingga madu digunakan sebagai peminimalisir efek samping. Penelitiaan ini adalah penelitian eksperimental yang dengan design “posttest-only control group design” yang bertujuan untuk mengetahui pengaruh fermentasi madu terhadap gambaran histologi jaringan pankreas akibat paparan cisplatin. Penelitian ini menggunakan 4 kelompok kontrol eksperimen dari tikus putih galur wistar jantan berumur 2,5 tahun sebagai populasi dan sampel penelitian yang akan diberikan fermentasi madu dengan variasi dua dosis (5% dan 10%) dan diamati selama 10 hari lalu diinduksi cisplatin dengan dosis 5ml/kgbb. Analisis data menggunakan one way ANOVA dan Uji Pos Hoc LSD. Hasil penelitian menunjukkan bahwa perbaikan gambaran histopatologi pankreas tikus terjadi pada kelompok 3 dengan pemberian fermentasi madu 5% dan luas pulau langerhans kelompok yang diberikan fermentasi madu 5% dan 10% memiliki luas yang paling mirip dengan kelompok normal. Sehingga menyimpulkan bahwa pemberian fermentasi madu 5% sebagai dosis efektif mampu memperbaiki jaringan pankreas. Dalam pandangan islam, pengobatan diperbolehkan dengan tujuan yang baik yaitu mengobati sebagaimana halnya cisplatin. Fermentasi madu sebagai pengobatan alami yang diciptakan Allah SWT dapat meminimalisir efek samping cisplatin.

Pengetahuan Kader Kesehatan tentang Tuberkulosis Wening Sari; M Restu Syamsul Hadi; Ndaru Andri Damayanti
Info Abdi Cendekia Vol 4 No 1: Juni 2021
Publisher : Lembaga Penelitian Universitas YARSI

Tuberkulosis (TBC) merupakan infeksi dengan angka kesakitan dan kematian yang tinggi. Salah satu program dalam penanggulangan TBC adalah penemuan kasus aktif secara luas untuk mendeteksi TBC agar pengobatan segera diberikan. Penjangkauan kasus yang belum terdeteksi di komunitas dapat dilakukan dengan memberdayakan masyarakat, khususnya kader kesehatan. Telah dilakukan kegiatan pengabdian kepada masyarakat berupa penyuluhan interaktif tentang penyakit TBC terhadap 30 orang kader kesehatan dari wilayah Cempaka Putih, Johar Baru, Kemayoran dan Sawah Besar Jakarta Pusat. Berdasarkan evaluasi pre-test dan post-test terdapat peningkatan pengetahuan kader kesehatan tentang TBC pasca penyuluhan (p=0,02). Setelah mempunyai pengetahuan yang lebih baik kader kesehatan diharapkan dapat mendeteksi gejala dan tanda penyakit TBC pada masyarakat sehingga penemuan suspek TBC meningkat.

Potential of Soursop Leaf Extract as an Antioxidant in MCF-7 Cells Mustofa, Muhammad Samsul; Hadi, Restu Syamsul; Rahmah, Nunung Ainur; Pendrianto, Pendrianto
Biology, Medicine, & Natural Product Chemistry Vol 13, No 1 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.131.265-269

The frequency of breast cancer tends to increase. Malondialdehyde (MDA) is a marker of oxidative stress as an end product from the chain reaction of lipid peroxidation. The use of traditional medicine soursopleaf (Annona muricata L.) has been reported for a long time because of its bioactivity as an antioxidant. This study analyzes the relationship between MDA levels and glutathione enzymes in MCF-7 cells given the methanol extract of soursop leaves. The methanol extract of soursop leaves was carried out by infusion method. The methanol extract of soursop leaves was given to cancer cells at several doses with an incubation of 24 hours. The cytotoxic test was carried out using the MTT method. Measurement of MDA levels was carried out using the thiobarbituric acid reactive substance (TBARS/TBA) reactivity test method. GSH measurements used the colorimetric method. The results showed that the ethanol extracts of soursop leaves have cytotoxic activity in the MCF-7 breast cancer cell line with IC50 values of 23.96 ppm. Ethanol extract of soursop leaves increased levels of MDA inhibition and GSH level. Soursop leaf extract could increase MDA inhibition GSH level in human breast cancer cells MCF-7.

The Effect of Honey Fermentation on the Cell Appearance in the Seminiferous Tubule Wall of Rats Induced by Cisplatin Ulviena, Lulu; Hadi, Restu Syamsul
Junior Medical Journal Vol. 2 No. 2 (2023): Oktober 2023
Publisher : Fakultas Kedokteran Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jmj.v2i2.3917

Background: Cisplatin is the most effective cancer treatment used in chemotherapy. Cisplatin works by inducing oxidative stress, but it also has toxic side effects on healthy cells in the male reproductive organs. Therefore, there is a need for a solution to reduce the side effects of cisplatin. Honey fermentation is a natural substance that can reduce the toxicity of cisplatin. Honey is known as “The food of God” which is a gift of sustenance from Allah SWT due to its extraordinary benefits. Methodology: This study utilized primary data with an experimental posttest-only control group design conducted in vivo. The research involved four experimental groups of rats: a control group without any treatment, a cisplatin group, a 5% honey fermentation group exposed to cisplatin, and a 10% honey fermentation group exposed to cisplatin. All rats were weighed before, during, and after the treatment. Subsequently, HE staining was performed for both descriptive and quantitative observations. Results: Honey fermentation can provide a protective effect on primary spermatocytes and Leydig interstitial cells, almost reaching normal levels. The highest improvement was observed in the 5% honey fermentation group for primary spermatocytes and the 10% group for Leydig interstitial cells. Conclusion: Honey fermentation administration can be protective for testicular organ cells against the side effects of cisplatin. From an Islamic perspective, Allah has created honey for consumption, as mentioned in Surah An-Nahl.

Effects of Green Tea Leaf Extract on Viability, Apoptosis, and Expression of Interleukin-6 in Keloid Fibroblasts Fasihah, Mariah Shofwah; Hadi, Restu Syamsul; Mustofa, Samsul
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 24, No 1 (2024): January
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v24i1.20146

Green tea leaf (Camellia sinensis) extract contains polyphenols, anti-inflammatory and anti-carcinogenic agents that may effectively to as keloid therapy. This study aims to examine the effect of green tea leaf extract on viability, apoptosis, and interleukin-6 expression in keloid fibroblasts. Keloid fibroblast cultures were divided into groups that were cultivated with green tea extract at dosages of 100, 200, 400, and 800 μg/mL, negative control (10% Fetal Bovine Serum/FBS), and positive control (100 μM dexamethasone). This study used post-test-only control group design. The highest mean IL-6 expression was in the GTE 200 (275,942.7 pg/mL), while the lowest was in the GTE 800 ( -2,890.6 pg/mL). The viability and apoptosis was analized by using one-way ANOVA, continued with Least Significant Difference (LSD) with a significance of p 0.05 in the GTE 800 treatment group compared to the entire intervention group. Kruskal-Wallis followed by a Mann-Whitney was carried out for interleukin-6 (IL-6) with a significance of p 0.05 in the extract therapy group at dosages of 200, 400, 800 μg/ml and 100 μM dexamethasone. In conclusion, green tea leaf extract at 800 μg/ml could reduce viability interleukin-6 expression and increase apoptotic caspase-3 expression in keloid fibroblast cells.

Green tea leaf extract reduces viability and migration of cholesteatoma fibroblast of chronic suppurative otitis media cultured in vitro Rizki, Anika; Suciati, Yulia; Hadi, Restu Syamsul
MEDISAINS Vol 21, No 2 (2023)
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/medisains.v21i2.17049

Background: Chronic Suppurative Otitis Media (CSOM) is still a health problem, especially in developing countries. CSOM with cholesteatoma is a dangerous type. Cholesteatoma in CSOM sufferers can cause various complications. Therefore, alternative therapies are needed, such as green tea leaf extract. Green tea leaf extract can be an antioxidant and anti-inflammatory, but its effectiveness in treating CSOM has not been studied before, so research is needed.. Purpose: This research aimed to determine the effect of green tea leaf extract that could reduce viability and migration in cholesteatoma fibroblast of CSOM.Methods: This research was an in vitro experiment with a post-test-only control group design. The sample for this research was cholesteatoma fibroblast cells obtained from the isolation of patients with CSOM. The method used is Hoechst staining for viability and scratch techniques for cell migration with eight groups of cholesteatoma fibroblasts consisting of a negative control group (DMEM+FBS), DMEM group, two positive control groups dexamethasone (10 µM and 100 µM), and four green tea leaf extract group (10 µg/ml, 40 µg/ml, 80 µg/ml, and 160 µg/ml). Data analysis used One-Way ANOVA and Kruskal Wallis tests.Results: The results showed that the highest average cell viability was in the negative control group (165.33), and the lowest was in green tea leaf extract at a dose of 160 µg/ml (70.88). Cell migration at 72 hours showed that in the negative control group, there was faster closure of the scratch area (97,78%) and the slowest closure on green tea leaf extract at a dose of 160 µg/ml (13,81%).Conclusion: Green tea leaf extract can reduce the viability and migration in cholesteatoma fibroblast of CSOM. It shows the potential of green tea extract as an alternative to prevent cholesteatoma.

Co-Authors Afif Ramdisa AGUNG ERU WIBOWO Akbar Kurniawan Anggi Puspa Nur Hidayati Anwar, Alifah Fitria Artha Budi Susila Duarsa Azmi, Sabrina CHURIYAH CHURIYAH Damayanti, Ndaru Andri Dewi, Lia Sari Utami Elmi Nuryati Elmi Nuryati Elza Rakhma Octaviaini Permana FAIZA KARA FABIOLA Fakhria Mutia Fasihah, Mariah Shofwah Fatimah Eliana, Fatimah Firman Arifandi Firman Arifandi Firman Arifandi Hakim, Jasir Indra Kusuma Indra Kusuma Indra Kusuma Indra Kusuma Irwandi, Nurmayani Juniarti . Juniarti Juniarti Kamal Anas Karimulloh, Karimulloh Kuslestari, K Kusumah, Indra Marsiati, Himmi Maudina Mahlani Mustofa, Muhammad Samsul Nadira, Nadira Ndaru Andri Damayanti Nunung Ainur Rahmah Nur Fadhilah Nur Fadhilah Parwanto, Edy Pendrianto Pendrianto, Pendrianto Pratiwi, Nike Rahmah Aprilia, Yoan Razari, Intan Reza Aditya Digambiro Rizki, Anika Rofilah, Dian Samsul Mustofa Samsul Mustofa Sembiring, Rinawati SISKA A KUSUMASTUTI Sofwan, Achmad TATI NURHAYATI Thamrin, Nabila Pyrenina TITIEK DJANNATUN Titiek Djannatun Tjandra Yoga Aditama Tri Panjiasih Susmiarsih Ulviena, Lulu Wening Sari Wira Alwysaputro Yulia Suciati Yurika Sandra

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