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All Journal MEDISAINS Biology, Medicine, & Natural Product Chemistry Mutiara Medika: Jurnal Kedokteran dan Kesehatan JURNAL BIOLOGI INDONESIA Universa Medicina Jurnal Kesehatan Vokasional Jurnal Ilmu Kefarmasian Indonesia YARSI Medical Journal Sumatera Medical Journal Majalah Kesehatan Pharmamedika Medika Alkhairaat : Jurnal Penelitian Kedokteran dan Kesehatan Medicra (Journal of Medical Laboratory Science/Technology) Proceeding ISETH (International Summit on Science, Technology, and Humanity) Junior Medical Journal Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)
Restu Syamsul Hadi
UNIVERSITAS YARSI
Religion Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Education Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Nicotine reduces cell viability and induces oxidative stress in human gingival fibroblasts Azmi, Sabrina; Hadi, Restu Syamsul; Kusuma, Indra; Suciati, Yulia; Sari, Wening
Universa Medicina Vol. 43 No. 1 (2024)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2024.v43.20-30

Abstract

BackgroundNicotine, as the main component of cigarettes, is known to interfere with the proliferation of human gingival fibroblasts (HGFs) and can trigger oxidative stress. This study aimed to analyze the impact of nicotine on viability, expression of the antioxidant Nrf2, levels of the product of oxidative stress malondialdehyde (MDA), and the migration capacity of HGFs. MethodsAn experimental laboratory study used fibroblasts isolated from healthy human gingiva. The cells were grouped into the non-treatment control group (NTC), the solvent control (SC), and the treatment groups, exposed to nicotine at various concentrations for twenty-four hours. Cell viability was assesed using the cell counting kit-8 (CCK-8), Nrf2 expression was examined using ELISA, MDA level was measured using an MDA kit, and migration capacity was assessed using a scratch assay. Statistical analysis used one-way Anova or Kruskal-Wallis test. A p-value of <0.05 was expressed statistically significant. ResultsThe Cell viability was substantially reduced in the nicotine group compared to the untreated group, accompanied by changes in cell morphology. In contrast, Nrf2 expression increased significantly (p=0.010) in the 5 mM nicotine group compared with the control group. The MDA levels were not significantly distinct across groups (p=0.056). Cell migration was delayed significantly in the 5 mM nicotine group at 72 hours after scratching compared to the control group. ConclusionNicotine decreased HGFs viability and increased Nrf2 expression significantly in a dose-dependent manner. Nicotine at 5 mM concentration did not alter MDA levels but delayed cell migration.
Effect of Green Tea Extract to the Degree of Knee Joint Damage and Nitric Oxide Levels in the Rabbit Osteoarthitis Model Susmiarsih, Tri Panjiasih; Hadi, Restu Syamsul; Sofwan, Achmad; Kuslestari, K; Razari, Intan
Proceeding ISETH (International Summit on Science, Technology, and Humanity) 2019: Proceeding ISETH (International Summit on Science, Technology, and Humanity)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/iseth.1438

Abstract

Osteoathritis (OA) is characterized by degeneration of articular cartilage, subchondral bone, synovial fluid and synovium. Nitric oxide (NO) is proinflammatory cytokine that play a significant role in the pathogenesis of OA via cartilage and bone degradation by synovial inflammation. Green tea is a novel functional food for treating osteoarthritis and inhibiting the production of inflammatory mediators such as nitric oxide. This study aimed to evaluate the effects of green tea extract to the nitric oxide levels and degree of knee joint damage in the rabbit osteoarthitis model. The Freud’s adjuvant complete was performed to induce OA, as many as sixteen male rabbits (New Zealand white) were randomly divided into four groups: adjuvant injection, adjuvant and green tea (injection), adjuvant and green tea (per oral), and control group. The control group only received drinking water, the Freud adjuvant (0.2 ml) and green tea extract (200 mg/kg bw) were orally and injection administered for eight weeks. The articular cartilage damage was evaluated histologically according to MANKIN score. NO levels were determined by nitric oxide assay. Data was analysed by Chi square test. The result of this study showed the surface structure damage of cartilage increased after adjuvant-induced. Green tea extract decrease significantly (p=0.02) the degree of knee joint damage after adjuvant-induced in rabbit osteoarthritis models. NO levels increased after OA induction. The green tea extract administration (via injection) can significantly (p = 0.038) decrease NO levels compare to adjuvant group. Green tea extract decrease the knee joint damage and NO proinflamantory levels in rabbit of osteoarthritis model
Pengaruh Fermentasi Madu terhadap Gambaran Histologi Pankreas pada Tikus Penderita Kanker yang Diinduksi Cisplatin dan Tinjauannya Menurut Pandangan Islam Hadi, Restu Syamsul; Novita Sari, Sonia; Marhamah, Siti; Mustofa, Samsul
Junior Medical Journal Vol. 3 No. 5 (2025): September 2025
Publisher : Fakultas Kedokteran Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jmj.v3i5.4628

Abstract

Kanker merupakan pertumbuhan sel yang tidak normal yang mana sel tersebut dapat tumbuh dan menyebar pada bagian tubuh lainnya bahkan dapat menyebabkan kematian. Cisplatin sebagai pengobatannya memiliki efek samping terhadap organ lain. Sehingga madu digunakan untuk meminimalisir efek samping dari cisplatin. Penelitian ini merupakan penelitian eksperimental dengan desain posttest-only control group dengan menggunakan tikus putih galur wistar jantan yang dibagi menjadi 5 kelompok. Sampel penelitian akan diinduksikan sel HSC-3 lalu diberikan dua dosis fermentasi madu (5% dan 10%) dan diamati selama 10 hari. Setelah itu diinduksikan cisplatin. Analisis data dilakukan menggunakan uji one way ANOVA dan uji Kruskal-Wallis. Hasil penelitian pada skoring kerusakan sel terdapat perbaikan pada kelompok 5 dengan pemberian fermentasi madu 10% dengan gambaran sel histologi pankreas adanya nekrosis dan degenerasi minim. Serta untuk luas pulau langerhans kelompok yang diberikan fermentasi madu 10% memiliki luas yang paling mirip dengan kelompok normal. Dari hasil penelitian dapat disimpulkan bahwa fermentasi madu 10% efektif untuk memperbaiki jaringan pankreas. Dalam pandangan Islam, wajib hukumnya untuk menjaga kesehatan. Fermentasi madu sebagai obat alami yang dituliskan dalam Surah An-Nahl dapat digunakan untuk meminimalisir efek samping dari penggunaan cisplatin.
Co-Authors AGUNG ERU WIBOWO Artha Budi Susila Duarsa Azmi, Sabrina CHURIYAH CHURIYAH Damayanti, Ndaru Andri Dewi, Lia Sari Utami Elmi Nuryati Elmi Nuryati FAIZA KARA FABIOLA Fasihah, Mariah Shofwah Fatimah Eliana, Fatimah Firman Arifandi Hakim, Jasir Indra Kusuma Indra Kusuma Irwandi, Nurmayani Juniarti . Juniarti Juniarti Kuslestari, K Kusumah, Indra Marsiati, Himmi Maudina Mahlani Mustofa, Muhammad Samsul Nadira, Nadira Novita Sari, Sonia Nunung Ainur Rahmah Nur Fadhilah Nur Fadhilah Parwanto, Edy Pendrianto Pendrianto, Pendrianto Pratiwi, Nike Rahmah Aprilia, Yoan Razari, Intan Reza Aditya Digambiro Rizki, Anika Rofilah, Dian Samsul Mustofa Samsul Mustofa Sembiring, Rinawati SISKA A KUSUMASTUTI Siti Marhamah, Siti Sofwan, Achmad TATI NURHAYATI Thamrin, Nabila Pyrenina TITIEK DJANNATUN Titiek Djannatun Tri Panjiasih Susmiarsih Ulviena, Lulu Wening Sari Yulia Suciati Yurika Sandra