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All Journal MEDISAINS Biology, Medicine, & Natural Product Chemistry Mutiara Medika: Jurnal Kedokteran dan Kesehatan JURNAL BIOLOGI INDONESIA Universa Medicina Jurnal Kesehatan Vokasional Jurnal Ilmu Kefarmasian Indonesia YARSI Medical Journal Jurnal Kesehatan Majalah Kesehatan Pharmamedika Medika Alkhairaat : Jurnal Penelitian Kedokteran dan Kesehatan Medicra (Journal of Medical Laboratory Science/Technology) Proceeding ISETH (International Summit on Science, Technology, and Humanity) Info Abdi Cendekia Junior Medical Journal Indonesian Journal of Multidisciplinary on Social and Technology Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)
Restu Syamsul Hadi
Fakultas Kedokteran Universitas YARSI
Religion Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Economics, Econometrics & Finance Education Engineering Health Professions Immunology & microbiology Library & Information Science Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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ANALISIS KOMPARATIF ATROFI SEREBRAL PADA GANGGUAN KOGNITIF NON-ALZHEIMER DAN PENYAKIT ALZHEIMER DI INDONESIA Digambiro, Reza Aditya; Marsiati, Himmi; Hadi, Restu Syamsul; Hastuty, Dewi; Parwanto, Edy
Medika Alkhairaat: Jurnal Penelitian Kedokteran dan Kesehatan Vol 6 No 3 (2024): Desember
Publisher : Fakultas Kedokteran Universitas Alkhairaat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31970/ma.v6i3.245

Abstract

Abstrak Penelitian ini menganalisis pola atrofi serebral pada pasien gangguan kognitif non-Alzheimer dan penyakit Alzheimer (AD) di Indonesia. Atrofi otak terjadi lebih cepat pada pasien AD dibandingkan penuaan normal, dan percepatan atrofi hipokampus ditemukan pada subjek dengan gangguan kognitif ringan (MCI) yang berkembang menjadi AD. Data diperoleh dari 710 subjek yang menjalani MRI di Rumah Sakit Cipto Mangunkusumo dan Rumah Sakit Hasan Sadikin. Analisis laju atrofi otak keseluruhan, hipokampus, dan pembesaran ventrikel menunjukkan perbedaan signifikan antara kelompok kontrol, MCI, dan AD. Rata-rata percepatan atrofi hipokampus pada subjek MCI mencapai 0,44% per tahun kuadrat (p < 0,05). Temuan ini menggarisbawahi pentingnya deteksi dini dan intervensi terapeutik pada tahap MCI. Penelitian ini juga menunjukkan bahwa pola atrofi serebral di Indonesia serupa dengan temuan global, meskipun terdapat perbedaan faktor risiko lokal. Penelitian lanjutan perlu mencakup analisis longitudinal jangka panjang untuk pemahaman yang lebih baik tentang progresi AD di Indonesia. Kata Kunci: Atrofi serebral, Alzheimer, gangguan kognitif ringan, Indonesia, hipokampus, MRI Abstract This study analyzes cerebral atrophy patterns in patients with non-Alzheimer cognitive impairment and Alzheimer's disease (AD) in Indonesia. Brain atrophy progresses faster in AD patients compared to normal aging, with accelerated hippocampal atrophy observed in mild cognitive impairment (MCI) subjects who progress to AD. Data were obtained from 710 subjects who underwent MRI at Cipto Mangunkusumo Hospital and Hasan Sadikin Hospital. Analysis of overall brain atrophy rates, hippocampal atrophy, and ventricular enlargement revealed significant differences between control, MCI, and AD groups. The average acceleration of hippocampal atrophy in MCI subjects was 0.44% per year squared (p < 0.05). These findings emphasize the importance of early detection and therapeutic intervention at the MCI stage. This study also indicates that cerebral atrophy patterns in Indonesia are similar to global findings, despite local risk factor variations. Further research should include long-term longitudinal analysis to gain a better understanding of AD progression in Indonesia. Keywords: Cerebral atrophy, Alzheimer’s disease, mild cognitive impairment, Indonesia, hippocampus, MRI
In vitro study: thymoquinone inhibits the proliferation and migration of keloid fibroblasts and increases their apoptosis Hadi, Restu Syamsul; Juniarti, Juniarti
MEDISAINS Early Release
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/medisains.v0i0.23975

Abstract

Background: No treatment can eliminate keloids. Thymoquinone (TQ) is hypothesized to play a pivotal role in treating keloids by modulating cellular processes such as proliferation, migration, and apoptosis. However, the existing studies investigating its effects on these mechanisms in keloid fibroblasts are limited and require further exploration.Objective: This study aims to investigate the effects of TQ on the proliferation, migration, and apoptosis of keloid fibroblasts in vitro.Methods: This experimental study was conducted using keloid fibroblast cultured in vitro. Cells were seeded in a 96-well plate at a density of about 5x103 cells per well with 100 μl of culture medium, and cells were cultured for 24, 48, and 72 hr for each concentration of TQ. Cell proliferation was assessed using a CCK-8 Kit, measuring optical density with a microplate reader. Apoptosis was measured using the TUNEL assay. Cell migration following TQ treatment was evaluated using the Scratch assay. The statistical test used a one-way analysis of variance (ANOVA) followed by the least significant difference (LSD) test. Results: TQ inhibited the proliferation of keloid fibroblasts at a dose of 5 µM after 48 hours of incubation and 10 µM after 24 hours of incubation. The inhibitory effect of TQ on fibroblast proliferation increased in a dose- and time-dependent manner. Treatment at 5 and 10 µM doses increased apoptosis in keloid fibroblast cultures. The TQ5 µM group achieved 60% closure, while the 10 µM group showed 55% closure. Migration was significantly inhibited in the 25 µM and 50 µM groups, with only 30% and 10% closure, respectively, at 72 hours.Conclusion: Thymoquinone inhibits the proliferation and migration of keloid fibroblast cells while promoting apoptosis. These properties suggest that TQ could be developed as a potential treatment for keloid-related skin issues.
Pengaruh Fermentasi Madu Terhadap Viabilitas dan Proliferasi Sel Fibroblast Pasca Diinduksi Cisplatin In Vitro Serta Tinjauan Menurut Islam Anwar, Alifah Fitria; Syamsul Hadi, Restu; Karimulloh
Junior Medical Journal Vol. 3 No. 1 (2024): September 2024
Publisher : Fakultas Kedokteran Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jmj.v3i1.4329

Abstract

Pendahuluan : Salah satu pengobatan kanker ialah dengan kemoterapi dan salah satu kemoterapi yang terkenal dan telah digunakan untuk berbagai pengobatan kanker ialah Cisplatin. Karena resistensi obat dan berbagai efek samping yang dapat terjadi, terapi kombinasi Cisplatin dengan obat lain harus dipertimbangkan untuk mengatasi resistensi obat dan mengurangi toksisitas. Metodologi : Jenis penelitian yang digunakan adalah penelitian eksperimental laboratorium dengan data yang akan diambil secara langsung dengan melakukan sebuah eksperimen/percobaan in vitro yang bertujuan mengerahui pengaruh fermentasi madu terhadap viabilitas dan proliferasi sel fibroblast pasca diinduksi cisplatin in vitro. Penelitian ini menngunakan 4 kelompok perlakuan dengan tiga kali pengulangan (triplicate) yang diberikan cisplatin dan fermentasi madu dengan konsentrasi 5% dan 10% dan diamati selama 24 jam. Analisis data yang akan digunakan ialah normalitas, one way ANOVA dan Uji Pos Hoc. Hasil : Fermentasi madu dapat memberikan efek pada viabilitas dan proliferasi sel fibroblast. Simpulan : penggunaan fermentasi madu untuk melihat pengaruhnya pada viabilitas dan proliferasi sel fibroblast pasca diinduksi cisplatin in vitro diperbolehkan karena sesuai dengan menjaga Al-Dharuriyat Al-Kham.
In vitro study: thymoquinone inhibits the proliferation and migration of keloid fibroblasts and increases their apoptosis Hadi, Restu Syamsul; Juniarti, Juniarti
MEDISAINS: Jurnal Ilmiah Ilmu-Ilmu Kesehatan Early Release
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/medisains.v0i0.23975

Abstract

Background: No treatment can eliminate keloids. Thymoquinone (TQ) is hypothesized to play a pivotal role in treating keloids by modulating cellular processes such as proliferation, migration, and apoptosis. However, the existing studies investigating its effects on these mechanisms in keloid fibroblasts are limited and require further exploration.Objective: This study aims to investigate the effects of TQ on the proliferation, migration, and apoptosis of keloid fibroblasts in vitro.Methods: This experimental study was conducted using keloid fibroblast cultured in vitro. Cells were seeded in a 96-well plate at a density of about 5x103 cells per well with 100 μl of culture medium, and cells were cultured for 24, 48, and 72 hr for each concentration of TQ. Cell proliferation was assessed using a CCK-8 Kit, measuring optical density with a microplate reader. Apoptosis was measured using the TUNEL assay. Cell migration following TQ treatment was evaluated using the Scratch assay. The statistical test used a one-way analysis of variance (ANOVA) followed by the least significant difference (LSD) test. Results: TQ inhibited the proliferation of keloid fibroblasts at a dose of 5 µM after 48 hours of incubation and 10 µM after 24 hours of incubation. The inhibitory effect of TQ on fibroblast proliferation increased in a dose- and time-dependent manner. Treatment at 5 and 10 µM doses increased apoptosis in keloid fibroblast cultures. The TQ5 µM group achieved 60% closure, while the 10 µM group showed 55% closure. Migration was significantly inhibited in the 25 µM and 50 µM groups, with only 30% and 10% closure, respectively, at 72 hours.Conclusion: Thymoquinone inhibits the proliferation and migration of keloid fibroblast cells while promoting apoptosis. These properties suggest that TQ could be developed as a potential treatment for keloid-related skin issues.
In Vivo Study of Telang Cream on Interleukin (IL)-6, Epidermal Growth Factor (EGF), and Wound Reepithelialization Pratiwi, Nike; Irwandi, Nurmayani; Hadi, Restu Syamsul; Thamrin, Nabila Pyrenina; Damayanti, Ndaru Andri; Juniarti, Juniarti; Rahmah, Nunung Ainur
Jurnal Kesehatan Vokasional Vol 10, No 2 (2025): May
Publisher : Sekolah Vokasi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jkesvo.106084

Abstract

Background: Povidone iodine is widely used in wound healing, but it can cause irritation and cellular toxicity. Clitoria ternatea L. has the potential to contain compounds such as rutin, quercetin 3-O-rhamnoside, ternatin, and anthocyanins as natural alternatives in wound healing. In this study, the extract used contains anthocyanins.Objective: The study aimed to evaluate the effects of Clitoria ternatea L. extract cream on the expression of Interleukin-6, Epidermal Growth Factor, and re-epithelialization in Sprague Dawley rat incision wounds.Methods: An in-vivo study with a post-test only control design was conducted on five treatment groups, including a control group without wounds (healthy), povidone iodine, and Clitoria ternatea L. extract cream (5%, 10%, 15%). IL-6 and EGF were analyzed using immunohistochemistry, while re-epithelialization was assessed using hematoxylin-eosin staining.Results: Clitoria ternatea L. extract increased IL-6 and EGF expression, with the highest expression at a 10% concentration. Re-epithelialization was faster in the treatment groups compared to both the positive and negative controls.Conclusion: The 10% Clitoria ternatea L. extract cream demonstrated efficacy comparable to povidone iodine in incision wound healing.
Pengaruh Fermentasi Madu Terhadap Ukuran Diameter Glomerulus Ginjal Tikus Penderita Kanker yang Diinduksi Cisplatin Hadi, Restu Syamsul; Rofilah, Dian
Junior Medical Journal Vol. 3 No. 3 (2025): Maret 2025
Publisher : Fakultas Kedokteran Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jmj.v3i3.4725

Abstract

Pendahuluan : Cisplatin sebagai pengobatan kemoterapi dinilai cukup efektif dalam berbagai terapi penyakit kanker. Cisplatin berinteraksi dengan DNA yang dapat menyebabkan terganggunya proses transkripsi DNA sehingga terjadi apoptosis baik pada sel kanker maupun sel-sel yang sehat. Hal ini menyebabkan timbulnya toksiksitas pada sel yang sehat, maka perlu adanya proteksi untuk menghambat efek samping cisplatin. Penggunaan fermentasi madu sebagai bahan alami dapat menurunkan efek samping cisplatin. Metodologi : Penelitian ini merupakan penelitian eksperimental secara in vivo menggunakan jenis desain posttest-only control group design. Penelitian ini memiliki 5 kelompok percobaan yaitu, kelompok kontrol tanpa perlakuan, kelompok kontrol yang diinduksi sel HSC-3, kelompok induksi sel HSC-3 dengan cisplatin, kelompok perlakuan fermentasi madu 5% dengan induksi sel HSC-3 dan paparan cisplatin, serta kelompok perlakuan fermentasi madu 10% dengan induksi sel HSC-3 dan paparan cisplatin. Setelah itu dilakukan pewarnaan HE dan dilakukan pengamatan secara kuantitatif. Hasil : Fermentasi madu dapat mempertahankan ukuran diameter glomerulus tetap normal. Simpulan : Fermentasi madu dapat dijadikan sebagai agen nefroprotektif terhadap efek samping cisplatin.
Proliferation, Migration, and Expression of Tumor Necrosis Factor-α from Green Tea Leaf Extract (Camellia sinensis) on Keloid Fibroblast Cells: Proliferasi, Migrasi, dan Ekspresi Tumor Necrosis Factor-α Ekstrak Daun Teh Hijau (Camellia sinensis) pada Sel Fibroblas Keloid Dewi, Lia Sari Utami; Hadi, Restu Syamsul; Eliana, Fatimah; Hakim, Jasir
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 8 No. 1 (2025): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v8i1.1769

Abstract

Keloid is a fibroproliferative dermal disorder caused by abnormal wound healing, characterized by excessive collagen deposition that extends beyond the wound boundaries. Keloids can cause pruritus and even pain. In addition to these two issues, keloids also diminish a person's quality of life due to aesthetic problems, especially if they appear on the face or other visible areas of the skin. Combination treatments for keloids are usually more successful than single treatments. Green tea leaf extract contains epigallocatechin-3-gallate polyphenols as anti-inflammatory agents. The aim of this study is to determine the potential of green tea leaf extract by observing the proliferation, migration, and expression of TNF-α in keloid fibroblast cells. Keloid fibroblast cells were divided into five treatment groups (TH100, TH200, TH400, TH800, and DEX100) and one negative control (DS). Cell proliferation was tested using a cell counting kit-8, migration was assessed using a scratch assay, and TNF-α expression was measured using an ELISA kit. All data were analyzed using SPSS, performing One-Way ANOVA, followed by Kruskal Wallis and Mann Whitney tests. The results showed that green tea leaf extract at a dose of 800 μg/mL significantly reduced the proliferation and migration rate of keloid fibroblast cells (p < 0.05). Meanwhile, in the TNF-α expression test, no significant difference was found in reducing TNF-α expression levels in keloid fibroblast cells (p > 0.05). Green tea leaf extract has the potential to be used as an alternative treatment for keloids as it reduces the proliferation and migration of keloid fibroblast cells. 
Perilaku Kader dalam Penemuan Suspek Tuberkulosis Fadhilah, Nur; Nuryati, Elmi; Duarsa, Artha; Djannatun, Titiek; Hadi, Restu Syamsul
Kesmas Vol. 8, No. 6
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Dukungan berbagai pihak meliputi perubahan perilaku masyarakat dan pemberdayakan masyarakat sangat diharapkan untuk penanggulangan tuberkulosis (TB). Pimpinan Pusat Muhammadiyah dan ‘Aisyiyah Provinsi Lampung terpanggil untuk bergerak bersama dalam program penanggulangan penyakit TB agar keberhasilan penanggulangan TB dapat tercapai. Penelitian ini bertujuan mengetahui berbagai faktor yang berhubungan dengan perilaku kader dalam menemuan suspek TB di Kabupaten Lampung Tengah. Penelitian dengan metode kuantitatif dan kualitatif ini menggunakan desain potong lintang, data primer dikumpulkan dari sampel 72 kader TB ‘Aisyiyah Kabupaten Lampung Tengah. Analisis dilakukan secara univariat, bivariat dengan menggunakan metode kai kuadrat, dan multivariat dengan regresi logistik. Penelitian ini menemukan lima variabel yang meliputi pengetahuan, sikap, pelatihan, dukungan pemegang program dan motivasi yang mendukung perilaku penemuan suspek. Tiga variabel yang meliputi pendidikan, pendapatan dan pekerjaan tidak mendukung perilaku penemuan suspek. Untuk meningkatkan penemuan suspek TB disarankan untuk lebih meningkat dukungan pengelola program yang berkelanjutan. The support of various parties, peoples behavior and empower communities in the implementation of TB countermeasures highly expected by Muhammadiyah Central Executive and Aisyiyah Lampung Province omit to move together in a tuberculosis prevention program for successful TB control can be achieved. This study aimed to determine the related factors of behavior cadres to detect suspected tuberculosis in Lampung districy middle. The quantitative and qualitative with study design a cross sectional was conducted using primary data on samples 72 Aisyiyah tuberculosis cadres Lampung district middle. The statistical analyses were performed by chisquare and logistic regression. The study results showed a significant five variable (support program managers, knowledge of cadre, motivation of cadre, attitude of cadre, training cadre) with the discovery suspected tuberculosis cases in Lampung Province. Logistic regression analysis found a good support program holders associated with the case of suspected tuberculosis. Program holders support is the most dominant factor of the discovery of suspected tuberculosis cases. Therefore the need for tangible support over again that the findings by the cadre suspected tuberculosis increased.
Nicotine reduces cell viability and induces oxidative stress in human gingival fibroblasts Azmi, Sabrina; Hadi, Restu Syamsul; Kusuma, Indra; Suciati, Yulia; Sari, Wening
Universa Medicina Vol. 43 No. 1 (2024)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2024.v43.20-30

Abstract

BackgroundNicotine, as the main component of cigarettes, is known to interfere with the proliferation of human gingival fibroblasts (HGFs) and can trigger oxidative stress. This study aimed to analyze the impact of nicotine on viability, expression of the antioxidant Nrf2, levels of the product of oxidative stress malondialdehyde (MDA), and the migration capacity of HGFs. MethodsAn experimental laboratory study used fibroblasts isolated from healthy human gingiva. The cells were grouped into the non-treatment control group (NTC), the solvent control (SC), and the treatment groups, exposed to nicotine at various concentrations for twenty-four hours. Cell viability was assesed using the cell counting kit-8 (CCK-8), Nrf2 expression was examined using ELISA, MDA level was measured using an MDA kit, and migration capacity was assessed using a scratch assay. Statistical analysis used one-way Anova or Kruskal-Wallis test. A p-value of <0.05 was expressed statistically significant. ResultsThe Cell viability was substantially reduced in the nicotine group compared to the untreated group, accompanied by changes in cell morphology. In contrast, Nrf2 expression increased significantly (p=0.010) in the 5 mM nicotine group compared with the control group. The MDA levels were not significantly distinct across groups (p=0.056). Cell migration was delayed significantly in the 5 mM nicotine group at 72 hours after scratching compared to the control group. ConclusionNicotine decreased HGFs viability and increased Nrf2 expression significantly in a dose-dependent manner. Nicotine at 5 mM concentration did not alter MDA levels but delayed cell migration.
Effect of Green Tea Extract to the Degree of Knee Joint Damage and Nitric Oxide Levels in the Rabbit Osteoarthitis Model Susmiarsih, Tri Panjiasih; Hadi, Restu Syamsul; Sofwan, Achmad; Kuslestari, K; Razari, Intan
Proceeding ISETH (International Summit on Science, Technology, and Humanity) 2019: Proceeding ISETH (International Summit on Science, Technology, and Humanity)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/iseth.1438

Abstract

Osteoathritis (OA) is characterized by degeneration of articular cartilage, subchondral bone, synovial fluid and synovium. Nitric oxide (NO) is proinflammatory cytokine that play a significant role in the pathogenesis of OA via cartilage and bone degradation by synovial inflammation. Green tea is a novel functional food for treating osteoarthritis and inhibiting the production of inflammatory mediators such as nitric oxide. This study aimed to evaluate the effects of green tea extract to the nitric oxide levels and degree of knee joint damage in the rabbit osteoarthitis model. The Freud’s adjuvant complete was performed to induce OA, as many as sixteen male rabbits (New Zealand white) were randomly divided into four groups: adjuvant injection, adjuvant and green tea (injection), adjuvant and green tea (per oral), and control group. The control group only received drinking water, the Freud adjuvant (0.2 ml) and green tea extract (200 mg/kg bw) were orally and injection administered for eight weeks. The articular cartilage damage was evaluated histologically according to MANKIN score. NO levels were determined by nitric oxide assay. Data was analysed by Chi square test. The result of this study showed the surface structure damage of cartilage increased after adjuvant-induced. Green tea extract decrease significantly (p=0.02) the degree of knee joint damage after adjuvant-induced in rabbit osteoarthritis models. NO levels increased after OA induction. The green tea extract administration (via injection) can significantly (p = 0.038) decrease NO levels compare to adjuvant group. Green tea extract decrease the knee joint damage and NO proinflamantory levels in rabbit of osteoarthritis model
Co-Authors Afif Ramdisa AGUNG ERU WIBOWO Akbar Kurniawan Anggi Puspa Nur Hidayati Anwar, Alifah Fitria Artha Budi Susila Duarsa Azmi, Sabrina CHURIYAH CHURIYAH Damayanti, Ndaru Andri Dewi, Lia Sari Utami Elmi Nuryati Elmi Nuryati Elza Rakhma Octaviaini Permana FAIZA KARA FABIOLA Fakhria Mutia Fasihah, Mariah Shofwah Fatimah Eliana, Fatimah Firman Arifandi Firman Arifandi Firman Arifandi Hakim, Jasir Indra Kusuma Indra Kusuma Indra Kusuma Indra Kusuma Irwandi, Nurmayani Juniarti . Juniarti Juniarti Kamal Anas Karimulloh, Karimulloh Kuslestari, K Kusumah, Indra Marsiati, Himmi Maudina Mahlani Mustofa, Muhammad Samsul Nadira, Nadira Ndaru Andri Damayanti Nunung Ainur Rahmah Nur Fadhilah Nur Fadhilah Parwanto, Edy Pendrianto Pendrianto, Pendrianto Pratiwi, Nike Rahmah Aprilia, Yoan Razari, Intan Reza Aditya Digambiro Rizki, Anika Rofilah, Dian Samsul Mustofa Samsul Mustofa Sembiring, Rinawati SISKA A KUSUMASTUTI Sofwan, Achmad TATI NURHAYATI Thamrin, Nabila Pyrenina TITIEK DJANNATUN Titiek Djannatun Tjandra Yoga Aditama Tri Panjiasih Susmiarsih Ulviena, Lulu Wening Sari Wira Alwysaputro Yulia Suciati Yurika Sandra