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TOLERANSI TANAMAN KEDELAI TERHADAP CEKAMAN AIR : AKUMULASI PROLIN DAN ASAM ABSISIK DAN HUBUNGANNYA DENGAN POTENSIAL OSMOTIK DAUN DAN PENYESUAIAN OSMOTIK Sopandie, Didy; Hamim, ,; Jusuf, Muhammad; Heryani, Nani
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 24 No. 1 (1996): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (991.078 KB) | DOI: 10.24831/jai.v24i1.1616

Abstract

In this experiment. the changes on leaf osmotic potential and accumulation of proline and abscisic acid were identified from drought-tolerant and drought - sensitive soybean genotypes. Three drought - tolerant (Mlg 2805, Mlg 2984 and Mlg 2999) and two sensitive soybean genotypes (Mlg 2510 and Mlg 3541) were subjected to drought condition created by regulating water supply in greenhouse.  The results revealed that exposing plants to drought stress brought about a decrease of leaf osmotic potential. The decrease of which was greater in drought-tolerant genotypes (6.91 to 10.11 bars) than in sensitive genotypes (0.55 to 0.69 bars). The decreasing of leaf osmotic potential was followed with increasing praline accumulation, especialy for Mlg 2805. Only Mlg 2805 showed the significant ABA accumulation when the plants were subjected to drought stress. It is suggested that the drought tolerance was associated with the reduction of leaf osmotic potential (osmotic adjusment) in which proline might play an important role. The role of ABA could not be clarified since there had been a great variability in ABA content of all tolerant genotypes.
Physiological and Biochemical Responses of Bisbul (Diospyros discolor Willd.) Seedlings to Varying Artificial Light at Night (ALAN) Rismona, Nurwani; Hamim, Hamim; Ning Tyas, Kartika; Damayanti, Frisca; Kusumaningtyas Pitaloka, Mutiara; Putri Maulida, Azkia; Triadiati, Triadiati
HAYATI Journal of Biosciences Vol. 33 No. 2 (2026): March 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.2.278-287

Abstract

Artificial light at night (ALAN) can significantly affect plant physiology, as many physiological processes are light-dependent. However, studies investigating the specific effects of ALAN on plants remain limited. This study aimed to assess the impact of ALAN on the growth and metabolite composition of bisbul (Diospyros discolor Willd.). The experiment was conducted in Nursery 2 of the Bogor Botanical Gardens over 12 months. A split-plot factorial design was employed with three replications, each consisting of three one-year-old seedlings. The main plots were assigned to light color treatments (control, red, green, and blue), subplots to light intensity levels (control, high, and low), and sub-subplots to illumination durations (0, 1, 6, or 12 hours) applied for 0, 2, or 7 nights/week. ALAN treatments were administered continuously for one year. The results indicated that blue light significantly increased leaf senescence, particularly under BH-6(2), BH-12(2), and BH-12(7) treatments. Conversely, high-intensity red light reduced plant height, shoot dry mass, photosynthetic rate, and chlorophyll content. Metabolite profiling revealed decreased levels of secondary metabolites such as caffeic acid and catechin, while compounds such as nicotinamide, L-proline, linolenic acid, and coumarin increased. These findings suggest that prolonged exposure (6-12 hours) to high-intensity red or blue light can disrupt circadian rhythms and impair physiological functions.
Efektivitas Teratai (Nelumbo nucifera G.) dengan Berbagai Media Tanam untuk Fitoremediasi Air Asam Tambang Raudhatunisya, Meuthea Najlaa; Mansur, Irdika; Hamim, Hamim
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 15 No 6 (2025): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Pusat Penelitian Lingkungan Hidup, IPB (PPLH-IPB) dan Program Studi Pengelolaan Sumberdaya Alam dan Lingkungan, IPB (PS. PSL, SPs. IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.15.6.963

Abstract

Acid mine drainage (AMD), characterized by high acidity and concentrations of heavy metals that can damage aquatic ecosystems, poses a serious environmental problem. This research aimed to analyze the effectiveness of Nelumbo nucifera Geartn. grown using a Floating Wetland System (FTW), treated with topsoil or bokashi, in altering pH and reducing heavy metals in coal mine AMD. The experiment was conducted for 14 days in a sedimentation pond of post-mining land at PT Bukit Asam, Palembang, Indonesia. Two FWS units were installed on the pond’s surface: one was enriched with topsoil, while the other was with bokashi. Fifteen N. nucifera plants were grown in each floating reactor, with plants grown directly in the AMD without the FWS used as the control group. Plantgrowth, media pH, and heavy metal contents were monitored during and after treatment. The results indicate that the system was capable of increasing the initial highly acidic AMD pH (pH 2.8) to a range close to neutral (6.5–6.9). The concentrations of Fe and Mn metals were significantly reduced through the absorption mechanism of roots, stems, and leaves, with an efficiency of more than 90%. XRD analysis also revealed the formation of secondary mineral phases that support vegetative growth in both reactors. These findings confirm that the FWS installed with the bokashi ameliorant and N. nucifera has great potential as a sustainable solution for acid mine drainage remediation. 
Jabon (Anthocephalus cadamba Roxb) Potency for Remediating Lead (Pb) Toxicity Under Nutrient Culture Condition Setyaningsih, Luluk; Setiadi, Yadi; Budi, Sri Wilarso; Hamim, Hamim; Sopandie, Didy
BIOTROPIA Vol. 25 No. 1 (2018): BIOTROPIA Vol. 25 No. 1 April 2018
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2018.25.1.712

Abstract

Information on metal adaptation in plants is essential to understand the mechanism of heavy metal remediation, including lead (Pb), mediated by forest plants in contaminated land. This study aimed to identify the adaptation mechanism of jabon (Anthocephalus cadamba Roxb.) seedlings to excessive lead levels based on the tolerance index of growth performance and lead transport to plant tissue. The seedlings were exposed to lead Pb(NO₃)₂​ at concentrations of 0, 0.5, 1, 1.5, 5, and 10 mM in nutrient culture for 15 days. The results showed that the tolerance index (TI) of the seedlings significantly decreased with Pb exposure up to 1.5 mM, although the TI values remained above 75%. All seedlings died at Pb concentrations of 5 mM and above. Lead accumulated in all parts of the seedlings, with the highest concentration found in the leaves (735.9 ppm) under 0.5 mM Pb exposure. Pb was transported to the upper portion of the seedlings, as indicated by a transport factor (TF) greater than 1. The results suggest that jabon can tolerate excessive Pb exposure up to 1.5 mM and has potential as a phytoremediator plant.
Growth, Photosynthesis and Proline Accumulation of Metal-Accumulator Weeds Hamim, Hamim; Apriani, Rani; Saprudin, Deden; Sulistyaningsih, Yohana C
BIOTROPIA Vol. 27 No. 3 (2020): BIOTROPIA Vol. 27 No. 3 December 2020
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.267 KB) | DOI: 10.11598/btb.0.0.0.1200

Abstract

Intensive use of heavy metals, particularly lead and mercury, in industrial activities and mining extraction has caused the widespread distribution of these contaminants, thereby threatening the environment. Consequently, the growth and several physiological responses of five metal-accumulator weed species were examined in response to mercury (Hg) and lead (Pb) treatments. The weed species Brachiaria mutica, Cyperus kyllingia, Ipomoea aquatica, Mikania micrantha, and Paspalum conjugatum were grown in water culture using half-strength Hoagland's solution and subjected to Hg(NO₃)₂ and Pb(NO₃)₂ at concentrations of 0, 0.25, and 0.5 mM for 3 weeks. Plant growth, photosynthesis, lipid peroxidation, and proline content were observed. Both Hg and Pb significantly reduced plant growth, but the decrease was more pronounced under Hg treatment than under Pb. Hg dramatically reduced the photosynthetic rate under different photosynthetic photon flux densities, indicating that Hg up to 0.5 mM damaged the photosynthetic apparatus in almost all species except I. aquatica. Hg and Pb treatments caused a dramatic increase in leaf MDA content, which was associated with a significant decrease in chlorophyll content. Most species were tolerant to Pb up to 0.5 mM except M. micrantha, while only C. kyllingia and I. aquatica were tolerant to Hg treatment up to 0.5 mM. Hg treatment also induced higher proline accumulation in leaves, although without a clear increment pattern among species, suggesting that proline may function as a stress alarm rather than as a tolerance indicator.
Co-Authors , Triadiati Abdul Aziz Ahmad Barizi ALEX HARTANA Alex Hartana Alex Hartana Anhikmah, Anhikmah Anja Meryandini Aris Tjahjoleksono Aris Tri Wahyudi Auliya, Fatikhatur Rizki BEKTI KURNIAWATI Cholili, Abd Hamid Damayanti, Frisca Deden Saprudin Devi Media Siska Dian Hendrayanti Didy Sopandie DWI ANDREAS SANTOSA Eddy Supriyono Ekoputro, Widiyatmo Faidah Faidah Fendiyanto, Miftahul Huda Hadisunarso Hadisunarso Hirmas Fuady Putra HIRMAS FUADY PUTRA, HIRMAS FUADY Iman Rusmana Inayatur Rosyidah Indah Puspita SARI Irdika Mansur khayati, Eliya Nur Kukuh Nirmala Kusumaningtyas Pitaloka, Mutiara Laelasari Laksmita Prima SANTI Luluk Setyaningsih Lutfiani Lutfiani Ma’arif, A. Samsul Ma’arif Miftahuddin Miftahudin , Miftahudin Miftahudin . Miftahudin, M. MISHBAHUDDIN, MOHAMAD Mubasyiroh, Mubasyiroh Muhammad Jusuf Munif Ghulamahdi Musawira N, Salwa Zahra Nampiah Sukarno Nani Heryani Naufal, Ahmad Himi Ning Tyas, Kartika NISA RACHMANIA MUBARIK Novalita Novalita Nugroho, Mulyanto Nugroho, Robertus Nur, Hasrul Satria Nurul Hidayati Palupi, Merry Fridha Tri Prihadi, Kususanto Ditto Priyo Sadono , Teguh Putri Maulida, Azkia Putri Nur Azizah Hutagalung Qurrota A`yun, Hajar Rani Apriani Rani Apriyani Raharja Ratnadewi, Yuliana Maria Diah Raudhatunisya, Meuthea Najlaa Rismona, Nurwani Rizka Rizka Robertus Nugroho Rosyidah, Risvi Uly Sadono , Teguh Priyo Sigit Tri Wibowo Sindiya, Vita Sri Wilarso Budi Suharsono Suharsono Sukmarayu P Gedoan Sukmarayu P. Gedoan Sulistyaningsih, Yohana C Sulistyaningsih, Yohana C Syaiful Mustofa Syuhadak, Syuhadak Tri Muji Ermayanti Tri Muji Ermayanti Turhadi Turhadi UTUT WIDYASTUTI UTUT WIDYASTUTI Utut Widyastuti W, Vika Ramadhina Wirantari, Ayu Puspa Yadi Setiadi Yanuar, Ai Ika Ayu Yohana Caecilia Sulistyaningsih Yuli Siti Fatma YULIN LESTARI Zeid B. Smeer