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4. Detection of Toxoplasma gondii infection by Polymerase Chain Reaction (PCR) and Histological Examination on Balb/c Mice Muhammad Hanafiah; Dwinna Aliza; Erdiansyah Rahmi; Wisnu Nurcahyo
The International Journal of Tropical Veterinary and Biomedical Research Vol 1, No 2 (2016): Vol. 1 (2) November 2016
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (300.131 KB) | DOI: 10.21157/ijtvbr.v1i2.6686

Abstract

The purpose of this research was to compare the use of PCR method and histological examination to diagnose toxoplasmosis in tissues of Balb/c mice infected with sporulated oocysts through drinking water. A total of 20 male Balb/c mice aged approximately 2 months were used in this experiment. Each mouse was infected with 1x103 Toxoplasma gondii tachyzoites intraperitoneally. Tissue samples (liver, lung, heart, kidney, and brain) were collected from 5 mice on day 1, day 5, day 7, and day 9 after infection. Samples were then examined by PCR and histological methods. The data collected were analyzed descriptively. The results showed that PCR method was more sensitive than histological examination. PCR examination using primer invitrogen gen can amplify DNA T. gondii at 436 bp of the samples from liver, lung, heart and brain on Day 7 and Day 9 after infection. The histological examination showed that the cyst of toxoplasma was found in the brain while mononuclear cells infiltration was found in other internal organs.
PENGARUH TINGKAT KEPADATAN POPULASI AYAM BROILER (Gallus sp.) DALAM KANDANG TERHADAP HIPERTROFI SEL KORTEKS ADRENAL (Influence of Population Density of Broiler Chickens (Gallus sp.) in Cages to the Hypertrophy of Adrenocortical Cells) Hamdani Budiman; Sugito Sugito; Widya Nanda; Muhammad Hanafiah; Muhammad Nur Salim; Ismail Ismail
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i2.3944

Abstract

The purpose of this study was to determine the effect of population density of broiler chickens (Gallus sp.) in the cages to the hypertrophy of adrenocortical cells. This study was conducted using complete randomized design (CRD) with 84 broiler chickens. All chickens were divided into 4 groups with 3 replications. Each group consists of 4 chickens (K1), 6 chickens (K2), 8 chickens (K3), and 10 chickens (K4). Chickens were rear from aged of 2 days up to 35 days. The histopathological examination of adrenal glands were performed by collected the adrenal glands of chickens then proceed to histopathological method and stained with haematoxylin eosin (HE). The percentage of hypertrophy of adreno cortical cells was calculated. The results of statistical analysis showed that the population densities of broiler chickens had highly significant effect (P0.01) to the histopathology of adrenal gland. The highest average of hypertrophy was found in K4. To conclude, the highest the dense population of broiler chickens in the cage, the highest the number of hypertrophy of adenocortical cells.Key words: population density, hypertrophy of adrenocortical cell
2. Identification of Avian Filarial Potential Vectors in Local Chicken (Gallus Domesticus) in Banda Aceh And Aceh Besar Nur Afriyanti; Yudha Fahrimal; Muhammad Hanafiah; Farida Farida; Rusli Rusli; Arman Sayuti
Jurnal Medika Veterinaria Vol 14, No 1 (2020): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v14i1.13887

Abstract

This study aimed at finding out the potential vectors of avian filariasis in four locations where chickens were found infected by avian filaria. The samples used in this study were blood-sucking insect vectors that were caught using a light trap. The four locations were namely Limpok, Lamreung, Tungkop (District of Aceh Besar), and Lampriet (Banda Aceh City). The insects obtained were dissected to find microfilaria in their bodies. The data obtained in this study were analyzed descriptively. The results obtained from the arrests were 141 mosquitoes and a total of 313 gnats. Based on the results of the identification of mosquitoes found in the four dominating locations, namely Culex spp. as much as (72.3%), while the gnat obtained were of the genus Culicodes spp. From the results of the mosquitoes and gnats section, microfilariae were not found. Based on the results of the study it can be concluded that from the four locations only two vectors were found, namely Culex spp. and Culicoides spp.
DAYA LARVASIDA EKSTRAK ETIL ASETAT DAUN KEMUNING (Murraya paniculata (L) Jack) TERHADAP LARVA NYAMUK Aedes aegypti Emi Minarni; Teuku Armansyah; Muhammad Hanafiah
Jurnal Medika Veterinaria Vol 7, No 1 (2013): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v7i1.2915

Abstract

Penelitian ini bertujuan mengetahui efektivitas larvasida ekstrak etil asetat daun kemuning terhadap larva Ae. aegypti. Materi yang digunakan dalam penelitian ini adalah ekstrak etil asetat daun kemuning (EEADK). Rancangan penelitian ini menggunakan rancangan acak lengkap (RAL) yang terdiri atas enam perlakuan yaitu P1 (100 ml aquades), P2 (abate 10%), P3 (EEADK 10 ppm), P4 (EEADK 50 ppm), P5 (EEADK 100 ppm), dan P6 (EEADK 1000 ppm). Pada masing-masing perlakuan ditambahkan 25 larva nyamuk Ae. Aegypti dan ragi sebagai makanan. Masing-masing perlakuan diulang sebanyak 3 kali ulangan. Hasil yang diperoleh dianalisis dengan menggunakan analisis varian (ANAVA). Rata-rata jumlah larva yang mati 24 jam setelah pemberian ekstrak pada kelompok P1; P2; P3; P4; P5; dan P6 masing-masing adalah 25,00±0,00; 0,00±0,00; 0,33±0,39; 1,00±0,00 ; 2,33±0,94; dan 3,33±0,47. Berdasarkan analisis uji Puncan menunjukkan bahwa terdapat perbedaan yang nyata (P0,05) antara kelompok perlakuan dengan kelompok kontrol. Pemberian ekstrak etil asetat daun kemuning dapat menurunkan jumlah larva Ae. Aegypti.
Penerapan Algoritma SHA-384 Pada Aplikasi Duplicate Video Scanner Hanafiah, Muhammad
Bulletin of Data Science Vol 2 No 2 (2023): February 2023
Publisher : Forum Kerjasama Pendidikan Tinggi (FKPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47065/bulletinds.v2i2.4499

Abstract

Cryptography is used to maintain the security of private information from anyone except those with the authority or secret keys to access encrypted information. However, with the rapid advancement of computer technology, challenges to information security are increasing. Video manipulation is one of the serious threats in the digital world today. Video manipulation is a common phenomenon where individuals can easily manipulate videos with harmful intent or to confuse others. One of the most dangerous types of video manipulation is the duplicate video scanner, in which a fake video is created to resemble the original video in many aspects, including color, shape, objects, and content, without significant changes from other parties. This can deceive many viewers and cause significant losses. In this research, the author proposes the use of the SHA-384 algorithm as a method to detect duplicate video scanners. This algorithm is used to generate a unique signature or hash from video data, which is then used to verify the authenticity of the video. The results of this research have great potential in addressing serious issues related to video manipulation. The ability to distinguish real videos from fake ones has a significant impact in various fields, including law, security, and information integrity. This research provides an effective solution to ensure video authenticity and can help combat the increasing fraud and manipulation in a complex digital environment.
Pembuatan jerami terfermentasi probion sebagai pakan alternatif pada ternak sapi terintegrasi di Gampong Blang Krueng Kecamatan Baitussalam Kabupaten Aceh Besar [Making probion fermented straw as an alternative feed in integrated cattle farming in Blang Krueng Village, Baitussalam Sub-District, Aceh Besar District] Sutriana, Amalia; Hanafiah, Muhammad; Melia, Juli; Aliza, Dwinna
Buletin Pengabdian Vol 4, No 3 (2024): Bull. Community. Serv.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpen.v4i3.41100

Abstract

Farmers, especially those in Blang Krueng Village, Baitussalam Subdistrict, Aceh Besar District, have so far been raising livestock using traditional or semi-extensive patterns, where livestock are released in pastures or rice fields to find their own feed and in the afternoon given grass that has been cut into pieces. The purpose of the activity is to assist farmers in their livestock business, especially in the problem of feed needs that still cannot be met. This activity is carried out using the PRA (Participatory Rural Appraisal) approach. In this case the target audience participates in increasing and analyzing their knowledge about their own conditions so that they can make plans and actions, especially in efforts to raise cattle. The method of processing fermented straw, among others, the straw is aerated so that the moisture content is 40%, the straw is stacked with a length of 2.5 m, a width of 2.5 m and a thickness of 25 cm, on top of the straw layer watered with water that has been mixed with urea until evenly distributed, on top of the straw layer sprinkled with starter until evenly distributed, the straw is stacked again with a thickness of 25 cm trampled until solid, repeated watering of water mixed with urea until evenly distributed, repeated sowing of starter until evenly distributed, so repeated until the pile can reach 3 m, after completion the top is covered with dry leaves such as banana leaves, rice straw is left for at least 3-4 weeks and fermented rice straw is ready to be given to livestock. The results of the service that have been achieved are the availability of alternative feed from fermented straw and can be given to cattle, where the cattle also want to consume it.
Sosialisasi pembuatan pakan silase pada kelompok ternak Sapi di Desa Alue Penyareng, Kecamatan Mereubo, KabupatenAcehBarat Pratama, Said Mirza; Wahyudi, Indra; Armia, Yasser; Ilham, Ilham; Hidayati, Nurul; Adam S, Koji Al; Hanafiah, Muhammad
Peternakan Abdi Masyarakat (PETAMAS) Vol 5, No 1 (2025): Volume 5, Nomor 1, Juni 2025
Publisher : Departemen of Animal Science, Agriculture Faculty, Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/petamas.v5i1.45991

Abstract

Kegiatan pengabdian kepada masyarakat ini bertujuan untuk memberikan sosialisasi mengenai pembuatan pakan silase kepada kelompok ternak sapi di Desa Alue Penyareng, Kecamatan Mereubo, Kabupaten Aceh Barat. Kegiatan dilaksanakan pada tanggal 28 April 2025 sebagai upaya peningkatan pengetahuan peternak tentang strategi penyediaan pakan hijauan yang berkelanjutan, terutama dalam menghadapi musim kemarau. Sosialisasi dilakukan melalui penyampaian materi secara lisan dan visual yang meliputi pengertian silase, manfaat penggunaan silase sebagai cadangan pakan, serta tahapan umum proses pembuatannya menggunakan bahan-bahan lokal yang mudah diperoleh. Hasil kegiatan menunjukkan antusiasme dan respon positif dari para peternak, yang ditunjukkan melalui diskusi aktif dan keinginan untuk mencoba teknologi silase di masa mendatang. Melalui kegiatan ini, diharapkan kelompok ternak memperoleh pemahaman awal yang kuat sebagai dasar dalam mengadopsi teknologi pakan yang lebih efisien dan berdaya simpan tinggi. Kegiatan sosialisasi ini menjadi langkah awal penting dalam mendukung keberlanjutan usaha peternakan rakyat di wilayah tersebut. Kegiatan lanjutan dalam bentuk pelatihan praktik dan pendampingan sangat dianjurkan agar teknologi silase dapat diimplementasikan secara efektif di lapangan.
Study of Toxoplasmosis Infection in Human and Related to Animal in Banda Aceh Hanafiah, Muhammad; Kamaruddin, Mufti; Nurcahyo, Wisnu; Winaruddin, Winaruddin
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i2.9813

Abstract

The research has been done to know prevalence of toxoplasmosis in livestock, to search the source of infection that have potency to cause toxoplasmosis in human and to make a map of toxoplasmosis infection area in Banda Aceh. The method used in this research was serologic test, Card Aglutination Test (CATT). Data of fertile women in this study are collected through questioner. The result of this research showed that the toxoplasmosis prevalency number of society in Banda Aceh was 3.15%, while at livestock were varied respectively: goats 40%, chickens 25%, cattles 23%, duck 20%, cats 16%, buffalos 15%, and sheeps 10%. The potencial sources of to coures toxoplasmosis in human are: goat, chicken, cattle, duck, cat, buffalo, and sheep. The area found toxoplasmosis in humans and animal were: Baiturrahman, Kuta Raja, Ulee Kareng, Kuta Alam, and Syiah Kuala. Model toxoplasmosis infection patterns in human related to livestock and animal in Banda Acheh are : Y ( POSTOKSO) - 1,55688 + 2,65280 AYA + 1,17709 JDG + 4,28482 KUC - 3,74609 MEM
Identification and Distribution of Species Culicoides spp. (Diptera: Ceratopogonidae) of Broiler in Banda Aceh Rusli, Rusli; Hanafiah, Muhammad
Jurnal Kedokteran Hewan Vol 4, No 1 (2010): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i1.9794

Abstract

A research has been done to identify the distribution of Culicoides species in Banda Aceh. Culicoides samples were collected by using modified Center for Disease Control (CDC) Miniatur Light Trap. All Culicoides samples has choice a small measured, were identified morfologically. The result showed that the number species Culicoides has caught in Ulee Kareng are: C. huffi, C. arakawae and C. shultzei, whereas in Alue Naga are C. huffi and C. shultzei. The distribution of species Culicoides in Ulee Kareng are: C. huffi has 22, C. arakawae 13 and C. shultzei 11. The average distribution of Culicoides in Alue Naga are C. huffi has 11 and C. shultzei has 4 .
PROFILE OF ALLERGY HYPERPLASMA PATHOLOGIC ANTIBODY AND IMMUNOGENIC CHARACTERISTIC Nazaruddin, Nazaruddin; Budiman, Hamdani; Gani, Basri A.; Jakfar, Subhaini; Hasan, M.; Hanafiah, Muhammad
Jurnal Kedokteran Hewan Vol 11, No 1 (2017): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i1.5299

Abstract

The objective of this research was to determine the allergy hyperplasma pathologic antibody and to evaluate the immunogenic characteristics. Blood and serum were collected from human suffering rhinitis, skin, eye and asthma allergies. To obtain a cloned allergen plasma protein (protein allergen that had been induced with the serum from human with allergic rhinitis, skin, eye and asthma), the goat was used as an intermediate animal. Hematological analysis showed that the leukocyte cell such as neutrophil, lymphocyte, monocyte, and eosinophil increase in allergic-suffered human. The blood smear test exhibited that the mastocyte cell was dominant which contributes to allergy activities in human body. The sodium dodecyl sulphate-polyacrylimide gel electrophoresis (SDS-PAGE) assay detected protein allergens with molecular weight of 188 kDa (IgE) and 60-62 kDa (mastocyte cell). The reactivity assay using enzyme linked immunosorbent-assay (ELISA) revealed that cloned-allergens (whole hyperplasma allergen from goat isolates) express the best reactivity at various concentrations of IgE than the leukocyte cells. This research concluded that the clones of protein allergen have better immunogenic characteristic and those proteins can be recommended as the candidate of allergen to induce the humoral immunity on host and deliver specific product of anti-allergy such as milk.