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AKTIVITAS ANTIPLASMODIUM EKSTRAK HIDROTROPI DAUN SAMBILOTO (Andrographis paniculata Ness.) SECARA In Vitro PADA Plasmodium falciparum STRAIN G-2300 RESISTEN KLOROQUIN Yance Anas; Rita Dwi Ratnani; Laeli Kurniasari; Indah Hartati
Jurnal Ilmu Farmasi dan Farmasi Klinik Vol 17, No 01 (2020): Jurnal Ilmu Farmasi & farmasi Klinik Vol 17 No. 01, Juni 2020
Publisher : Universitas Wahid Hasyim Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (388.354 KB) | DOI: 10.31942/jiffk.v17i01.3479

Abstract

ABSTRACT The bitter leaf extract (Andrographis paniculata Ness.) has long been known as an anti-malarial. Our previous research has developed a bitter leaf hydrotrophy extract (BLHE) using a sodium acetate 2 mol/L as a hydrotop compound solution. The extraction process lasts for 2 hours, faster than conventional extraction. The result is two types of BLHE with different stirring temperatures, at 30°C (BLHE1) and 35°C (BLHE2). This study meant to determine and compare the in vitro antiplasmodial activity BLHE1 and BLHE2. The anti-plasmodial activity of BLHE1 and BLHE2 (0.1- 50.0) g/mL performed on a G-2300 strain of P. falciparum (chloroquine-resistant) after incubated for 48 hours (triplicate). The percentage of parasitemia determined through an examination of blood smears stained with Giemsa. The percentage of growth inhibition of P. falciparum evaluates by comparing percentage of growth with the control group and IC50 determine by probit analysis. The results showed that BLHE1 and BLHE2 respectively were able to inhibit the growth of P. falciparum of 14.62% - 43.25% (IC50: 59.689 μg/mL) and 24.06% - 49.48% (IC50: 44.541 μg/mL). Based on these data, BLHE should produce with sodium acetate 2 mol/L, with stirring at 35°C, because this condition will obtain BLHE that active as anti-plasmodial. Keywords: Anti-plasmodial, bitter leaf hydrotrophy extract, P. falciparum strain G-2300, in vitro
STANDARDISASI SPESIFIK DAN NON SPESIFIK EKSTRAKSI HIDROTROPI ANDROGRAPHOLID DARI SAMBILOTO (Andrographis paniculata) Rita Dwi Ratnani; Indah Hartati; Yance Anas; Devi Endah P.; Dita Desti D_Khilyati
Jurnal Ilmu Farmasi dan Farmasi Klinik Prosiding Seminar Nasional "Peluang Herbal Sebagai Alternative Medicine"
Publisher : Universitas Wahid Hasyim Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.478 KB) | DOI: 10.31942/jiffk.v0i0.1358

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ABSTRACT Several active compounds has been found in Andrographis paniculata, including andrographolide (deoxy andrographolide, andrographolide, neo andrographolide and 14-deoxy-11,12-didehydroandrographolide) and kalmeghin. The active compounds properties and its levels in extract medicinal plants cannot be guaranteed to always be in a constant amount. This may be due to variations in the quality of medicinal plants, such as seeds, grow location, climate, conditions (age and harvest method), as well as post-harvest process and extraction method. Therefore, standardization of the extract should be performed to ensure the quality of the extract before it is produced on an industrial scale. The purpose of this study is to standardize the sambiloto hydrotropic extract. Specific parameters such as the organoleptic properties of the extract and identification of andrographolide compound as marker compounds. In contrast, the non-specific parameter includes drying shrinkage, microbiological contamination levels, heavy metal contamination levels, ash levels, the solubility of extracts in water and ethanol. The results showed that the organoleptic properties hydrotropic sambiloto extract are in powder form dense, dry, dark green, characteristic odor and bitter taste. Andrographolide compound as a marker compound in the sambiloto extract was identified by TLC method. The non-specific parameters of sambiloto hydrotropic extract were shrinkage drying of 13.5%, microbiological contamination of (3.1x107) CFU/g, heavy metal contamination (Cd levels of 0.104 ppm, and Pb levels of 2.248 ppm), ash levels of 37.5%, and the solubility extracts in water and ethanol are 40.8 % dan 42.0% respectively. Key words: Andrographis paniculata, extract standardization, specific and non-specific
HEAVY METALS ADSORPTION BY XANTHATE OF FOOD PROCESSING WASTE Indah Hartati; Indah Riwayati
Techno (Jurnal Fakultas Teknik, Universitas Muhammadiyah Purwokerto) Vol 12, No 2 (2011): Techno Volume 12 No 2 Oktober 2011
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (204.283 KB) | DOI: 10.30595/techno.v12i2.37

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Industrial effluent loaded with heavy metals are a cause of hazard to human and other forms of life. Conventional methods such as precipitation, evaporation, electroplating, ion exchange, membrane process used for removal of heavy metals from waste water however, are often cost prohibitive having inadequate efficiencies at low metal ion concentrations. Adsorption technology has become one of the alternative treatments. It is observed that appropriated modification of the raw adsorbents especially xanthates can eliminate the drawbacks of adsorbent and improve their performances significantly. Xanthates are most prominent because they are highly insoluble, easy to prepare with relatively inexpensive reagents and have high stability constant values of the metal complexes formed. Xanthates are formed by reacting an organic hydroxyl containing substrate with carbon disulfide under caustic condition. One of organic hydroxyl-containing substrate that can be used for xanthation is food processing waste. The major factors affect the biosorption process are: contact time, initial metal ion concentration, temperature, pH & biomass concentration in solution. It is postulated that metal ion adsorption process could undergo a complex formation. Most of heavy metal adsorption into the xanthate compound followed Langmuir adsorption isotherm model, and well described by the pseudo-second- order equation. Key-word : Adsorption, Xanthate, Heavy metal
PEMANFAATAN SUSU SAPI PERAH MELALUI DIVERSIFIKASI PRODUK OLAHAN MENJADI SABUN MANDI SUSU (IPTEKS BAGI MASYARAKAT PETANI-PETERNAK DI DESA TERWIDI GUNUNG PATI SEMARANG) Indah Riwayati; Indah Hartati; Mr. Darmanto; Endah Subekti
Techno (Jurnal Fakultas Teknik, Universitas Muhammadiyah Purwokerto) Vol 16, No 1 (2015): Techno Volume 16 No 1 April 2015
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/techno.v16i1.66

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Tujuan dari kegiatan ini adalah untuk memberikan solusi bagi permasalahan mitra kelompok tani ternak sapi perah yakni: (i) memperbaiki proses produksi berupa aplikasi alat perajang/pencacah rumput dan singkong, (ii) memberikan pelatihan produksi diversifikasi produk susu sapi yakni sabun susu sapi disertai dengan aplikasi alat mixer yang digunakan dalam proses produksi sabun susu sapi, (iii) memberikan pelatihan desain kemasan, (iv) memberikan pelatihan manajemen usaha kecil, dan (v) memberikan pelatihan perijinan. Adapun target kegiatan adalah perbaikan proses produksi melalui aplikasi mesin perajang rumput dan singkong, pelatihan proses produksi sabun susu sapi, penggunaan alat mixer pada proses produksi sabun susu sapi, pelatihan desain kemasan sabun susu sapi, pelatihan manajemen usaha, dan pelatihan perijinan. Metode yang digunakan guna mencapai tujuan adalah sebagai berikut: (i) perbaikan proses produksi akan dilakukan dengan merancang, mempabrikasi alat perajang rumput oleh tim pengusul bersama mitra serta menguji coba alat. Pabrikasi dilakukan di Workshop tim pengusul, (ii) pelatihan proses produksi sabun susu sapi dilakukan dengan tahapan berupa pelatihan, uji coba alat dan pendampingan yang dilaksanakan melalui ceramah, diskusi dan praktek; (iii) pelatihan desain kemasan akan dilakukan hingga mendapatkan prototype desain kemasan sabun susu sapi yang baik dan menarik. Pelatihan dilaksanakan melalui ceramah, diskusi dan praktek; (iv) pelatihan manajemen usaha akan dilakukan guna memberikan dasar-dasar manajemen usaha dan strategi pemasaran. Pelatihan dilaksanakan melalui ceramah, diskusi dan praktek; dan (v) pelatihan perijinan dilakukan melalui ceramah dan diskusi sehingga mitra memahami prosedur permohonan ijin suatu produk. Kata kunci : ipteks, sabun, susu, sapi
PRODUCTION OF LOW METHOXYL PECTIN AS AN ANTI CANCER AGENT FROM CITRUS PEEL PECTIN THROUGH ENZYME DEMETHYLATION BY PAPAYA PECTINESTERASE Indah Hartati; Indah Riwayati; Laeli Kurniasari
Techno (Jurnal Fakultas Teknik, Universitas Muhammadiyah Purwokerto) Vol 12, No 1 (2011): Techno Volume 12 No 1 April 2011
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (221.436 KB) | DOI: 10.30595/techno.v12i1.29

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Low methoxyl pectin (LMP) is reported posses anti cancer activity. LMP administering could reduce the risk of cancer, halt the progression of cancer, and in a certain percentage of cases caused the cancer cells to start to die. If this can be developed further, LMP administration could be a good co-treatment for chemotherapy or radiation. This would be a positive advancement, due to the high toxicity to the body of both chemotherapy and radiation. LMP can be produced by demethylation of high methoxyl pectin. One of our local resources that is potential for its pectin content is citrus peel. Pectin demethylation can be conducted by acid, alkali, ammonia in alcohol or enzymatic method. LMP produced by enzyme demethylation have been found to be inferior in quality to those produced by other methods. The enzyme took part in the pectin demethylation is pectinesterase. Pectinesterase can be be isolated from various source such as fruit and vegetables. One of our local resources that is potential as source of pectinesterase is papaya. Considering facts above thus it has a great possiblity to produce LMP from citrus peel pectin through enzymatic demethylation by utilize pectinesterase of papaya. Key-word : Low methoxyl pectin pectin (LMP), anti cancer, citrus peel pectin, papaya, pectinesterase
PEMURNIAN ENZIM SELULASE DARI RUMEN SAPI MENGGUNAKAN TEKNOLOGI EXPANDED BED ADSORPTION Indah Hartati
Techno (Jurnal Fakultas Teknik, Universitas Muhammadiyah Purwokerto) Vol 13, No 1 (2012): Techno Volume 13 No 1 April 2012
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (478.749 KB) | DOI: 10.30595/techno.v13i1.43

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Keluasan lingkup penggunaan enzim telah mendorong pertumbuhan pasar dan produksi enzim. Salah satu jenis enzim hidrolase yang memiliki pangsa pasar cukup besar adalah selulase. Saat ini produksi enzim selulase dari ruminansia belum banyak dilirik. Namun demikian selulase yang berasal dari ruminansia, seperti cairan rumen sapi, sangat berpotensi untuk diproduksi mengingat ketersediaan sumber isolat yang besar. Potensi limbah cairan rumen sapi di Indonesia mencapai 54,25 juta liter/tahun pada tahun 2007. Isolat enzim dari rumen sapi disebutkan memiliki berbagai kelebihan dibandingkan enzim komersial, diantaranya, lebih stabil pada suhu tinggi, aktivitas spesifik lebih tinggi, pH optimum lebih tinggi dan biaya produksi yang lebih rendah. Proses pemurnian enzim merupakan tahapan krusial dalam proses produksi enzim. Salah satu teknik pemurnian protein adalah expanded bed adsorpsion (EBA). EBA adalah teknik kromatografi pemisahan dan pemurnian produk biologi langsung dari umpan, tanpa melalui proses sentrifugasi, mikrofiltrasi dan tahap penjernihan lainnya. Mengingat unggun pada kolom dapat terekspansi, maka luas permukaan kontak adsorben lebih besar sehingga interaksi adsorben dengan molekul target lebih efektif. Dalam proses pemurnian protein menggunakan EBA, perlu ditelaah karakteristik ekspansi dan hidrodinamikanya, seperti indeks Richardson-Zaki (n), bilangan Bodenstein (Bo) dan koefisien dispersi aksial (Daxl) yang mewakili kondisi dispersi cairan dan sifat fluidisasi. Kata Kunci: selulase, cairan rumen sapi, EBA.
ANALISA KELAYAKAN USAHA PRODUKSI PERMEN JELLY KOLANG KALING DI LIMBANGAN KENDAL Indah Hartati; Nugroho Widiasmadi; Renan Subantoro
Techno (Jurnal Fakultas Teknik, Universitas Muhammadiyah Purwokerto) Vol 17, No 1 (2016): Techno Volume 17 No 1 April 2016
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/techno.v17i1.76

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Kolang kaling merupakan salah satu produk dari tanaman aren. Peningkatan nilai ekonomi usaha pengolahan kolang kaling dapat dilakukan melalui diversifikasi produk kolang kaling dalam bentuk permen jelly kolang kaling. Penambahan kolang kaling pada formulasi pembuatan permen jelly kolang kaling merupakan salah satu usaha untuk mengurangi penggunaan senyawa pengental yang selama ini digunakan dalam proses produksi permen jelly. Kajian kelayakan usaha produksi permen jelly kolang kaling memiliki peran stategis dalam pengembangan usaha diversifikasi produk kolang kaling. Hasil perhitungan analisa finansial menunjukkan bahwa dari perhitungan laba/rugi rencana investasi usaha akan menghasilkan laba bersih sebesar Rp 592.035,00/bulan. Hasil perhitungan menunjukkan bahwa usaha permen jelly kolang kaling akan mencapai titik impas (BEP) apabila memproduksi dan menjual permen jelly kolang kaling sebanyak 41,7 kemasan atau senilai Rp 687.925 Kata kunci: permen jelly, kolang kaling, analisis financial
Pengaruh Suhu Microwave Asissted Extraction (MAE) Dalam Proses Delignifikasi Limbah Kulit Nanas Dengan Pelarut Aquades untuk media optimasi produksi biogas Safaah Nurfaizin; Indah Hartati
Prosiding Seminar Sains Nasional dan Teknologi Vol 12, No 1 (2022): VOL 12, NO 1 (2022): PROSIDING SEMINAR NASIONAL SAINS DAN TEKNOLOGI
Publisher : Fakultas Teknik Universitas Wahid Hasyim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36499/psnst.v12i1.7394

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Negara Indonesia memiliki banyak biomass sebagai salah satu sumber energi. Satu diantaranya limbah kulit nanas memiliki kandungan selulosa cukup besar yang bisa dimanfaatkan sebagai sumber bahan baku energi terbarukan. Akan tetapi adanyan lignin dalam kulit nanas menjadi penghambat dalam proses degradasi sel. Sehingga perlu dilakukan penelitian mengenai proses optimasi selulosa limba kulit nanas dengan dengan metode delignifikasi MAE (microwave asissted extraction). Metode MAE memanfaatkan pancaran gelombang mikiro terhadap bahan polar sehingga terjadi interaksi antar molekul atau rotasi dipol yang menghasilkan panas sehingga dapat membantu memecah struktur bahan yang komplek menjadi struktur yang lebih sederhana. Dari hasil penelitian yang telah dilakukan dengan memvariaskan suhu operasi pada 70oC, 80oC dan 90oC didapatkan hasil bahwa kelarutan lignin meningkat seiring dengan kenaikan suhu operasi. Sampel kontrol yang digunakan sebagai pembanding memperoleh kelarutan lignin dan selulosa sebesar 2,68% dan 17,39% dari berat sampel. Sedangkan pada sampel yang dilakukan proses delignifikasi MAE mampu menaikan kelarutan lignin dan selulosa mencapai 9,04% dan 29,42% dari berat sampel pada suhu operasi 90oC, waktu 20 menit dan rasio 1:20 (b/v) yang menjadi kondisi optimum operasi delignifikasi MAE dengan variabel suhu. Sedangkan pada suhu 700C dan 800C secara berurutan mampu melarutkan  28.1955 %, 28.1389 % selulosa dan 7.4248 %, 7.3909 lignin.
Kinetic Studies of the Glycerolysis of Urea to Glycerol Carbonate in the Presence of Amberlyst-15 as Catalyst Hary Sulistyo; Wahyudi Budi Sediawan; Reviana Inda Dwi Suyatno; Indah Hartati
Bulletin of Chemical Reaction Engineering & Catalysis 2021: BCREC Volume 16 Issue 1 Year 2021 (March 2021)
Publisher : Masyarakat Katalis Indonesia - Indonesian Catalyst Society (MKICS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.9767/bcrec.16.1.8893.52-62

Abstract

Amberlyst-15, a strong acidic ion-exchange resin, has showed as a potential and an effective catalyst for the glycerolysis process of urea to glycerol carbonate. In this work, the kinetic model of the urea glycerolysis over Amberlyst-15 catalyst was investigated. The kinetic model was developed by considering simultaneous steps of urea dissolution in glycerol, mass transfer of urea and glycerol from the bulk of the liquid into the outer part of the catalyst, diffusion of urea and glycerol into the inner part of the particle through the catalyst pores, and irreversible second order reaction of urea and glycerol on the active sites. The irreversibility of second order reaction of urea glycerolysis was validated and proven. The proposed kinetic model was simulated and validated with the experimental data. The kinetic studies show that mechanism proposed works well. Furthermore, the activation energy was found to be 145.58 kJ.mol−1 and the collision factor was in 8.00×1010 (m3)2.kg−1.mol−1.s−1. The simulation result shows that the predicted liquid temperatures were close to the experimental temperature data. It also gave glycerol concentration profile inside the catalyst particle as a function of glycerolysis time and position. Copyright © 2021 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0). 
Apocarotenoids: Sources, Classification and Their Potential Application as Food Additives Indah Hartati; Vita Paramita
Research in Chemical Engineering Vol. 2 No. 2 (2023): September
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/rice.v2i2.112

Abstract

Apocarotenoids are the degradation product of the enzymatic and or non enzymatic cleavage of carotenoids. Apocarotenoids are present in plants, fungi, bacteria and a certain animal species. There have been large numbers of apocaretonoids identified as the consequence of the great variety of naturally occurring carotenoids. Apocarotenoids can be found as volatile, semi-volatile or non-volatile compounds based on their chemical structures, while based on the number of the carbon atoms contained in their structure, apocarotenoids is classify into triporoids, cyclofarnesoids and methylhexanoids. Bibliometric analysis performed on publication having “apocarotenoids” keywords informs that crocin, pirocrocin, saffron and crocus sativus nodules appear in the overlay visualization. The appearance is attributed to the great potential application and commercialization of crocin which is originated from saffron. The bibliometric analysis also shows that there is food additive nodule appear which imply the strong correlation and potential application of apocarotenoids as food additive. The potential utilization and the commercial production of apocarotenoids are challenged by the the genetic diversity as well as the high cost, time consuming and seasonal production of natural apocarotenoids isolation process. Chemical synthesis, engineering biosynthesis and bio-manufacturing using microbes provide a promising and economical alternative.
Co-Authors . Widayat Achmad Wildan Aditya Alfan Marzuki Aisyah Hana Rifiani Aji, Bayu Prasetyo Anggun Titi Nurcahyati Aniq, Nur Anita Dwi Puspitasari Aprilia Putri Sulistianingrum Aqnes Budiarti Ardi, Pradipta Risma Rukma Ari Yuniastuti Arie Setya Putra Arifah, Difa Al Ariyani, Sinta Aryanti, Desi Rahma Asari, Yunita Dwi Auviatita, Faeza Azzahra Aulia Hanifa Azzahra Aulia Hanifah Bella Paramaeshela Broto, R. T. D. Wisnu Chandra Pribadi Darmanto Darmanto Darmanto Darmanto Darmanto Darmanto Dea Ayu Ade Arisma Deddy Kurniawan Wikanta Deddy Prihadi Devi Endah P. Devina Ingrid A. Devita Hardianti Dewi Susanti Dewi Susanti Dian Risdianto Dita Desti D_Khilyati Diyono Ikhsan Dwi Handayani Dwi Kurnia Indar Dyah Puspa Arum Dyah Yuliana Zulfa Efa Firmania Eflita Yohana Endah Lestari Endah Subekti Ernawati Budi Astuti Fachry Abda El Rahman Fahmi Arifan Faizin, Safaah Nur Farikha Maharani Faris Hermawan, Faris Farizki, Bangkit Fatnawati Nur Hidayah Februana Hutavia Purba Caraka Fifi Kurniasari Fitriani, Dea Syifa fitriyani, Ulfa Fuji Utami Habib Abdun Nafik Hamid Aqil Hamid Aqil Hani Haswati Hardiyanti Amalia Harianingsih, Harianingsih Hary Sulistyo Hary Sulistyo Hary Sulistyo Hasan, Hasan Haswati, Hani Helmy Purwanto Hendriyanto Hendriyanto Hidayatun Natijah Imam Syafaat Indro Sumantri Irawan, Bei Harira Ismi Yatun Ismiyatul Kholisoh Ismiyatul Kholisoh Ismiyatul Kholisoh Julianto, Mohamad Endy Khasanah, Vina Nafidzatul Khornia Dwi Lestari Lailatul Firdaus Laeli Kurniasari Laeli Kurniasari Laeli Kurniasari Laeli Kurniasari Laeli Kurniasari Laeli Kurniasari Lailatul Fitriyah Latif, Abdullah Maharani Kusumaningrum Malikah, Anna Irdatul Maria Ulfah Marlina, Lala Adetia Mauluddin, M. Subchan Mauludin, Mochamad Subchan Mega Kasmiyatun Milzam, Muhammad Mira Nurhayani Mochamad Arif Budihardjo Moh Fahrurrozi Moh Fahrurrozi Mohamad Endy Y Yulianto Mohammad Endi Yulianto Mr. Darmanto Muhammad Dzulfikar Muhammad Mufti Azis Munifilia Ekasari Mustagfirin Mustagfirin Mustaghfirin Mustaghfirin Nafik, Habib Abdun Nazaruddin Sinaga Novika Aszyiamilatul Utami Nugroho Widiasmadi Nugroho, Agung Nur Aniq Prihastuti, Putri Putry, Cindyana Renan Subantoro Renan Subantoro Reviana Inda Dwi Suyatno Rita Ade Lasria Pardede Rita Dwi Ratnani Safaah Nurfaizin Salsa Erna Setiawati Sari, Ratmi Rahma Sarmi Sarmi Sekar Apriana Sinta Ariyani Siti Faizatul Mutiqoh Siti Soimah Siti Sudarmiseh Sofa Rohmania Sufrotun Khasanah Supriyadi Supriyadi Sury Widiyanti Suwardiyono Suwardiyono Suwardiyono Suwardiyono Suwarno Suwarno Tabah Priangkoso Tandang Patria Tama Tisa Lazuardy Titis Puspitasari Titis Puspitasari Udin Bahrudin, Udin vifta, rissa laila Vita Paramita Vita Paramita Vita Paramita Wahyudi Budi Sediawan Wahyudi Budi Sediawan Wahyudi Budi Sediawan Widarti Widarti Wulan Budi Astuti Yance Anas Yapan, Ulfa Fitriyani Zahroh El Baidho Zakiyyatut Darojah Zakka Athoo’ Illah Zulfa, Dyah Yuliana