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All Journal JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology GEMA TEKNOLOGI METANA Jurnal Perikanan dan Kelautan Journal of Marine Research BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Kelautan Tropis JMM (Jurnal Masyarakat Mandiri) Marine Research in Indonesia Jurnal Ilmiah Samudra Akuatika Journal of Tropical Marine Science Budimas : Jurnal Pengabdian Masyarakat Economics and Digital Business Review Journal of Applied Geology Jurnal Suara Pengabdian 45 Prosiding Seminar Nasional Unimus Journal of Fisheries & Marine Jurnal Perikanan dan Kelautan Cakrawangsa Bisnis

Retno Hartati

Department Of Marine Science, Faculty Of Fisheries And Marine Science, Diponegoro University. Tembalang Campus, Semarang, Indonesia Tel. / Fax. +6224 7474698

Author-ID : 276837

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Nursing Physics Public Health Social Sciences Veterinary Other

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Kandungan Lipid Total Nannochloropsis oculata Pada Kultur dengan Berbagai Fotoperiod (Total Lipid Content of Culture of Nannochloropsis oculata at Different Photoperiod) Widianingsih Widianingsih; Retno Hartati; Endrawati Hadi; Valentina R Iriani
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 17, No 3 (2012): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Fotoperiod memainkan peranan penting dalam proses fotosintesis pada mikroalga Nannochloropsis oculata. Fotoperiod akan mempengaruhi kadar total lipid pada berbagai jenis mikroalga. Penelitian ini bertujuan untuk determinasi kandungan lipid total mikroalga Nannochloropsis oculata yang dikultur pada berbagai kondisi fotoperiod Rancangan acak lengkap dengan tiga ulangan diterapkan pada penelitian ini. Perlakuan pada penelitian ini adalah fotoperiod 4 jam terang-20 jam gelap, 8 jam terang-16 jam gelap, 12 jam terang-12 jam gelap, dan 24 jam terang. N. oculata dikultur pada erlenmeyer 250 mL dengan sistem aerasi kontinyu dan pencahayaan 3000 lux, salinitas 33 ppt dan medium Conway. Hasil penelitian menunjukkan bahwa kandungan lipid total pada N. oculata tertinggi ditemukan pada perlakuan fotoperiod 12 jam terang-12 jam gelap) (31,8±3,03%-dw) dan kemudian diikuti pada fotoperiod 8jam terang - 16 jam gelap (25,2±2,19%-dw) dan fotoperiod 24 jam terang (23,2 %-dw). Pada perlakuan fotoperiod 8 jam terang-16 jam gelap dan fotoperiod 12 jam terang-12 jam gelap pada media pemeliharaan N.oculata menunjukkan hasil kadar lipid total yang lebih besar pada fase stasioner dibandingkan dengan eksponensial.Kata kunci: Nannochloropsis oculata, total lipid, fotoperiodPhotoperiod has important role on the photosynthesis process of microalgae Nannochloropsis oculata. Photoperiod also influences on total lipid content of various microalgae. This research has purpose to examine the effect of nutrient phosphate and nitrate composition to the total lipid content of N. oculata belong to class Eustigmatophyceae. There were four treatments of photoperiod as follows; (a) photoperiod A (4:20 hour light:dark);(b) photoperiod B (8:16 hour light:dark); photoperiod C (12:12 hour light:dark); (and (d) photoperiod D (24 hour light). There were three replicates for each treatment. The volume of culture medium was 250 mL for each treatment with continuously aeration and illumination (3000 lux). According to the research, the highest total lipid content of N. oculata had been found on the treatment of photoperiod 12:12 hour light:dark 31,8 ± 3,03 %-dw, and then followed by photoperiod 8:16 hour light dark as amount 25,2 ± 2,19 %-dw and 24 hour light (23,2 ± 1,99%-dw), and then followed by photoperiod 24 hour light. Differences of photoperiod 8:16 hour light:dark and photoperiod 12:12 hour light:dark on culture medium of N. oculata showed result that there were differences of total lipid content on the stationary and exponential phase. The highest percentage of total lipid was fund in cell of N. oculata grown under treatment of photoperiod 12:12 hour light:dark. The treatment of photoperiod 8:16 hour light:dark and 12:12 hour light:dark in the N. oculata culture showed result that the total lipid content on stationary phase was greater than exponential phase.Key words: Nannochloropsis oculata, total lipid, photoperiod

Asexual Reproduction of Black Sea Cucumber from Jepara Waters Retno Hartati; Muhammad Zainuri; Ambariyanto Ambariyanto; Sri Redjeki; Ita Riniatsih; Ria Azizah; Hadi Endrawati
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 24, No 3 (2019): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Black sea cucumber or Lollyfish are trade name for Holothuria atra, one species of family Holothuriidae abundance in Jepara waters, especially in Panjang Island. They inhabit on the seabed, in shallow waters on reefs and sand flats or in Seagrass meadows. Beside reproduce sexually, H. atra also do fission (biology), i.e. able to reproduce asexually by transverse fission. Monthly survey has been conducted for three months to determine frequency of fission among H. atra population in Panjang Island waters. In total 891 individu of H. atra inhabit in seagrass meadow mixed with rubble were examimed. In fissiparous sea cucumber, transverse fission are followed by regeneration and in this research identified from external observations. The fission state was divided into three category, i.e. closed wound (Category 1), little regeneration either posterior or anterior part (category 2), moderate regeneration either posterior or anterior part (category 3). The result showed that the fission frequency was highest during end of rainy season in April (13,21%) and decreased during May (4,61%) and June (4,86%). Body regeneration seem happened fast, since the new individu sea cucumber at category 1 was low but high as category 3. The regeneration were related with the condition of environment.

Relationship Between Stage of Gonad Maturity and Level of Osmotic Work of Sea Cucumber, Paracaudina australis Widianingsih Widianingsih; Muhammad Zainuri; Sutrisno Anggoro; Hermin Pancasakti Kusumaningrum; Retno Hartati
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 23, No 1 (2018): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

The holothuroid spawning can be succesful depend on varoius factors such as salinity, temperature, primary productivity and other physiological adaptation toward environment influences. The salinity fluctuation is one of the environment factors, which influence and can cause various osmotic pressure of coelomic fluid of Holothoroid and also gonad maturity stage. Holothuroid (sea cucumber), Paracaudina australis, is overexploited in Kenjeran waters, Surabaya, East Java. The study was aimed to reveal relationship between gonad maturity stage and level of osmotic work of sea cucumber P. australis. Fifty samples of P. australis were collected monthly. Coelomic fluid samples of approximately 200–1000 µl were obtained from all samples using disposable insulin syringes. There were 37 holothuroids classified in the late maturity (stage 5), 74 holothuroid in the earlier maturity stage (stage 1). Gonad maturity stage 5 (late maturity) could be reached on December 2016. Contrasly, the gonad maturity stage 1 (earlier maturity) was found on August 2016. The analysis of coelomic fluid showed that the average value of level of osmotic work ranged 70 to 91 mOsm/L H2O in the earlier gonad maturity stage. Contrasly, in the late maturity, the average value of level of osmotic work ranged 118 to 156 mOsm/L H2O. There is relationship between gonad maturation stage and level of osmotic work. From stage of gonad maturity 1 to 5, there were upward trend level of osmotic work in coelomic fluid of P. australis.

Fission Reproduction of Two Stichopudidae Species (Holothuria:Echinodermata) (Reproduksi Fission dari Dua Species Teripang Famili Stichopudidae (Holothuria:Echinodermata) Retno Hartati; Widianingsih Widianingsih; Pradina Purwati
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 18, No 2 (2013): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Terdapat dua sistem reproduksi pada teripang, yaitu seksual dan aseksual. Fission, sebagai salah satu cara reproduksi aseksual, adalah kemampuan alami teripang untuk membelah tubuhnya menjadi dua bagain dan masing-masing bagian dapat beregenerasi menjadi individu baru. Fenomena ini memberikan kemungkinan melakukan simulasi fission untuk memperbanyak benih teripang. Penelitian ini bertujuan untuk menganalisa kemampuan fission dua species Stichopudidae (Stichopus hermanii and S. chloronotus) dari Karimunjawa. Simulasi fission dilakukan dengan mengikat teripang uji dengan karet pentil dan meletakkan individu teripang yang telah diikat pada keranjang yang digantung pada permukaan laut. Pengamatan fission dilakukan pada jam ke 12, 24 dan 48. Ketika telah terjadi fission dan luka tertutup, teripang uji tetap berada di keranjang uji dan teripang tidak diberi pakan. Perlakuan berlanjut sampai penyembuhan secara morfologis terjadi dengan sempurna. Waktu terjadinya regenerasi setelah luka sembuh dicatat sebagai data pengamatan. Hasil penelitian menunjukkan bahwa simulasi fisssion dapat dilakukan pada species teripang Stichopudidae. Setelah proses fission selesai, dinding tubuh pada lokasi fission menjadi basah atau berlendir selama satu sampai dua jam. Pada pagi hari berikutnya, dinding tubuh akan nampak normal konsistensinya dan luka telah tertutup. Proses fission berlangsung selama beberapa jam sampai dua haridan tanda-tanda regenerasi timbul mulai dua sampai tiga minggu setelah fission. Waktu generasi S. hermanii terjadi lebih cepat (2 minggu setelah fission) daripada S. chloronotus. Kata kunci : fission, produksi benih, stichopudidae, Pulau Karimunjawa There are two reproduction systems in seacucumber, i.e. sexual and asexual. Fission, as a way of asexual reproduction, is natural seacucumber ability to split their body into two part, and this natural fenomenon give possibility to conduct fission stimulation as seacucumber propagation. Present works are aimed to analyse fission capability of two Stichopudidae species of sea cucumber (Stichopus hermanii and S. chloronotus) from Karimunjawa Islands. Fission stimulation by rubber band tied was done in basket hanged on the sea cage. The fission was observed for 12, 24 and 48 hours. When fission occurred and wound recovered the sea cucumber were still kept in the basket and no food was added. This treatment continued until morphological recovery was completed. The time regeneration occurred after wounds recover was recorded. The result of present work revealed that stichopudidae species showed very good response to fission stimulation. After the entire process of fission, the bodywall at the fission site remained a liquid or mucus like consistency for at least two more hours. The following morning, the bodywall had its normal consistency and the wounds at both ends were nearly entirely closed. The fission process took several hours to two days and signs of regeneration appeared two to five weeks after fission. Fission simulation give shorter regeneration time for S. hermanii (2 week) than S. chloronatus. Keywords: fission, seed production, stichopudidae, Karimunjawa island

Identifikasi Sand Dollar dan Karakteristik Habitatnya di Pulau Cemara Besar, Kepulauan Karimunjawa Jepara Anastasia Dian R; Retno Hartati; Ambariyanto Ambariyanto
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 10, No 1 (2005): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Sand dollar (Echinoidea, Echinodermata) memiliki bentuk tubuh ireguler, pipih dan tidak mempunyai lengan. Sand dollar banyak ditemukan di daerah Intertidal, terutama pada daerah berpasir. Penelitian ini betujuan untuk mengetahui jenis Sand dollar dan karakteristik habitatnya di Pulau Cemara Besar Kepulauan Karimunjawa Jepara. Penelitian dilaksanakan pada bulan September – Oktober 2003. Analisa sampel dilaksanakan di Laboratorium Ilmu Kelautan Teluk Awur, Jepara. Metode yang digunakan dalam penelitian ini adalah metode deskriptif. Sedangkan pengambilan sampel dilakukan dengan metode survey. Walaupun hasil penelitianmenunjukkan hanya satu spesies Sand dollar yang ditemukan dalam penelitian ini yaitu Laganum laganum, namun terdapat 10 variasi corak pada permukaan aboralnya. Sand dollar yang ditemukan pada sisi barat pulau lebih banyak dibandingkan yang ditemukan pada sisi timur, hal ini diduga karena adanya perbedaan karakteristik habitat. Karakteristik habitat Sand dollar di sisi barat adalah 48% pasir berflora dengan dengan kandungan bahan organik rata-rata 3,88%. Sedangkan karakteristik habitat di sisi timur 52% hamparan pasir dengan dengan kandungan bahan organik rata-rata 2,88%.Kata kunci : Sand dollar, variasi corak, habitatSand dollar (Echinoidea, Echinodermata) have an irregular body shape, thin and they do not have any arms. These animals can be found in intertidal area with sandy bottoms. The present study aims to investigate the natural populations of Sand dollar at Cemara Besar island, Karimunjawa and its relation with its habitat. This study was done on September – October 2003, and samples were analysed at Teluk Awur Marine Laboratory, Jepara. The results showed that there was only one single species of sand dollar found in the area, i.e. Laganum laganum. Within this spesies there were 10 variation of aboral surface pattern. There was also differences on the Sand dollar abundance between west and east side of the island, where higher number of sand dollars were dound at west side os the island. It also found that the bottom habitat of west side consists of 48% sand with flora, and 3,88%. While at east side consists of 52% sand and an average of 2,88% organic content.Key words : Sand dollar, aboral surface pattern, habitat

Composition of Juvenile Corals on Different Morphotypes of Substrate at Karimunjawa Archipelago, Indonesia Munasik Munasik; Diah Permata Wijayanti; Retno Hartati; Rudi Pribadi; Yoko Nozawa
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 4 (2016): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Patterns of coral recruitment are partially explained by fitness consequences of substrate selection, and may be affected by substrate morphology. This study examined juvenile coral assemblages on morphologically different dead coral substrate in shallow water reefs (4-5 m) at Karimunjawa Island, Java Sea (Indonesia). Surveys on juvenile corals were performed using 0.5×0.5 m transects on two different morphotypes of natural substrate; tabular dead corals (stable) and branching dead corals (unstable). Results showed that the morphological characteristics of dead coral substrate had a significant influence on the generic composition of juvenile corals. Coral recruits on tabular dead coral substrate (stable) which composed micro-cervices was more diverse than on branching dead coral substrate (unstable). Juvenile corals of the genus Acropora were dominant on (exposed microhabitats of) tabular dead coral substrate, while those of the genus Fungia and Montipora were dominant on (cryptic microhabitats of) branching dead coral substrate. These results suggest that Acroporid juveniles can besettled to the various morphotypes of the substrate, thus the distribution pattern of juvenile corals in shallow reef Karimunjawa may correlate with the distribution pattern of natural substrates.Keywords: juvenile, microhabitat, dead coral, substrate Karimunjawa Archipelago

Kajian Gonad Teripang Getah (Holothuria vagabunda) pada Saat Bulan Penuh dan Bulan Baru di Perairan Bandengan, Jepara Retno Hartati; Heri Yanti
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 11, No 3 (2006): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Banyak faktor lingkungan yang diduga mempengaruhi aspek reproduksi Teripang Getah (Holothuria vagabunda) antara lain siklus bulan. Penelitian ini bertujuan untuk mempelajari tingkat kematangan gonad teripang getah (H. vagabunda) pada saat bulan penuh dan bulan baru. Pengambilan sampel dilaksanakan delapan kali pada saat bulanpenuh dan bulan baru (Januari-April 2004) di perairan Bandengan, Jepara. Terhadap gonad dilakukan pengamatan tingkat kematangan gonad; jumlah, percabangan, panjang dan diameter tubula; jumlah sakula, nilai IKG serta fekunditas dan diameter telurnya. Hasil penelitian menunjukkan bahwa gonad H. vagabunda saat bulan penuhberada pada stadia II-III sedang saat bulan baru berada pada stadia II-V. Jumlah, percabangan, panjang, dan diameter tubula, jumlah sakula serta nilai IKG meningkat seiring dengan meningkatnya TKG dan mencapai puncaknya pada stadia IV untuk kemudian menurun pada stadia V. Diameter telur dan fekunditas juga meningkat seiringdengan meningkatnya TKG dan mencapai puncaknya pada stadia IV. Nilai rata-rata IKG H. vagabunda tertinggi diperoleh pada saat bulan baru demikian pula dengan diameter telur dan fekunditas terbesar. Gonad H. vagabunda mencapai puncak perkembangannya pada saat bulan baru dan pemijahannya diperkirakan terjadi pada saat itu.Kata kunci gonad, Holothuria vagabunda, bulan penuh, bulan baruThe Common black teat (Holothuria vagabunda) is one of marine resources in Indonesia with high economic value. Many environmental factors such as moon phase are assumed influencing their reproduction aspect. Thisresearch aimed to learn the common black teat (H. vagabunda) gonad during full and new moon. The samples were taken during full and new moon (January-April 2004) at Bandengan coastal waters, Jepara. The gonad samples were examined for gomad maturity stages; number, branch, length and diameter of tubula; number of saccula, IKG as well as fecundity and oocites diameter. The results showed that during full moon, H. vagabunda gonad were at stage II and III. Number of branch, length, sacculae, diameter of tubules and GI value increase as gonad maturity stage raised and the peak obtained at stage IV. The same result also happended for their oocites diameter and fecundity. The highest Gonadal Maturity Index (GMI), the biggest oocites diameter and fecunditywas reached during new moon and it’s predicted they were spawned during that time.Key words: gonad, Holothuria vagabunda, full moon, new moon

Histologi Gonad Kerang Totok Polymesoda erosa (Bivalvia : Corbiculidae) dari Laguna Segara Anakan, Cilacap Retno Hartati; Ita Widowati; Yoki Ristadi
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 10, No 3 (2005): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Kerang Totok (Polymesoda erosa) merupakan jenis bivalvia yang banyak ditemukan di kawasan hutan mangrove di Segara Anakan, Cilacap. Tujuan penelitian ini adalah untuk mengetahui struktur gonad kerang totok pada beberapa tingkat kematangan gonad melalui studi histologis. Enampuluh sampel kerang totok diambil dari Pulau Gombol, Laguna Segara Anakan, Cilacap, selama bulan Mei sampai Agustus 2002 untuk diamati gonadnya secara makroskopis dan histologis. Hasil penelitian menunjukkan bahwa pada sampel kerang jantan dan betina ditemukan tiga tingkat kematangan gonad dan stadia gonad yang Belum dapat diidentifikasi jenis kelaminnya. Diameter oosit meningkat sejalan dengan tingkat kematangan gonadnya dengan kisaran 38 – 100 μm. Diameteroosit rata-rata pada tigkat kematangan gonad I, II dan III berturut-turut adalah 58,8 μm; 66,4 μm and 77,2 μm.Kata kunci : Histologi, gonad, tingkat kematangan gonad, Polymesoda erosaMangrove clam, Polymesoda erosa is an economically valuable bivalvia species which is commonly consumed and has a potential to be cultured in Indonesia. The aim of this research was to understand gonad structure ofthe clams at different gonad maturity stages by histologycal study. The samples were taken monthly on May to August 2002 at Gombol iskand of Laguna Segara Anakan, Cilacap. Sixty samples were used examinevisually for gonad maturity and then were studied histologically. The results of present works revealed that there were 3 gonad maturity stage of the samples both at female and male samples as well as unidentifiedsex samples. Diameter of oocytes increased following the maturity of the gonad. The rage of diameter oocytes wer 38 – 100 μm. Average diameter of oocyte during gonad maturity stage 1, 2 and 3 were 58,8 μm; 66,4 μm and 77,2 μm respectively.Key words : Histology, gonad, maturity stage, Polymesoda erosa

Penempelan Larva Teripang Putih (Holothuria scabra) pada Substrat yang Berbeda Retno Hartati; Widianingsih Widianingsih; Delianis Pringgenies; Nur Taufiq; Ali Djunaedi; Sari Budi Moria
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 7, No 3 (2002): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Metamorfosis pada larva teripang diikuti dengan perubahan tingkah lakunya. Stadia auricularia bersifat planktonic sedangkan stadia akhir doliolaria dan awal pentactula bersifat bentik dan memerlukan substrat untuk menempel. Perubahan ini merupakan saat kritis dalam pembenihan teripang sehingga substrat yang sesuai perlu diberikan pada media pemeliharaan larva. Penelitian ini bertujuan untuk mengetahui kesuakaan penempelan larva teripang putih (H. scabra) pada substrat asbes, kasa plastik dan kaca. Penghitungan densitas larva yang menempel pada tiap substrat dilakukan sejak larva mulai menempel pada hari ke-25 hingga larva mencapai stadia juvenil-umur 35 hari. Hasil penelitian menunjukkan bahwa substrat memberikan pengaruh yang berbeda nyata (P>0.05) terhadap jumlah larva yang menempel. Larva lebih banyak menempel pada substrat asbes (rata-rata 425,28 individu.m2 dengan kelulushidupan 9,96 % tetapi substrat kaca paling sedikit ditempeli larva (85,18 individu.m2 dengan kelulushidupan 3,98%). Perbedaan jumlah larva yang menempel pada perlakuan substrat diduga berkaitan dengan kekasaran permukaan substrat.Kata kunci: larva teripang putih; penempelen; substrat The metamorphoses of seacucumber larvae indicate changing in their behaviour. Auricularia stage is planktonic but late doliolaria and early pentactula are benthic and need suitable substrate. It is a critical period in development and culture of seacucumber larvae since the biggest mortality happened during metamorphosis and settlement therefore the suitable substrata need to be provided. The aims of the experiment was to determine larvae's preference on substrate made of asbestos, plastic screen and glass. Density of larvae settled on the substare were counted on day 25 up to juvenile stage of 35 days old. The results showed that the treatment gave significant differences on larvae settlement on the substrate. The larvae found attached on asbestos was highest (425,28 ind.m2 and survival rate 9.96 %) and lowest number larvae settled on glass (85,18 ind.m2 and survival rate 3,98 %). It seemed due to difference roughness of surface.Keywords: larvae seacucurnber; settlement; substrats

Biomassa dan Estimasi Simpanan Karbon pada Ekosistem Padang Lamun di Pulau Menjangan Kecil dan Pulau Sintok, Kepulauan Karimunjawa Retno Hartati; Ibnu Pratikto; Tria Nidya Pratiwi
Buletin Oseanografi Marina Vol 6, No 1 (2017): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Isu blue carbon telah menjadi perhatian dunia, melalui konsep UNEP 2009 yang telah memasukan vegetasi padang lamun sebagai penyerap karbon di lautan. Penyerapan karbon yang disimpan melalui sedimen dan jaringan pada lamun dalam bentuk biomassa. Penelitian yang dilakukan di Pulau Menjangan Kecil dan Pulau Sintok, Karimunjawa bertujuan untuk melihat tingkat kerapatan, tutupan dan penyerapan karbon yang tersimpan dalam biomassa jaringan lamun (akar, rhizoma dan daun). Kerapatan serta tutupan lamun diukur dengan melakukan sampling lapangan menggunakan metode transek kuadrat 1m x 1m, identifikasi jenis lamun melihat panduan dari buku seagrasswatch. Hubungan kerapatan, biomassa dilakukan untuk melihat nilai kandungan karbon pada lamun. Sampling kerapatan, tutupan lamun dan nilai biomassa dilakukan pada semua titik, sedangkan untuk analisa karbon pada metode pengabuan dilakukan pada titik 50 m yang kemudian dikonversikan dengan nilai biomassa pada titik lainnya. Hasil pada penelitian ini ditemukan 8 jenis lamun, yaitu Enhalus acoroides, Thallasia hemprichii, Cymodocea rotundata, Cymodocea serrulata, Halodule uninervis, Halophila ovalis, Halophila minor, dan Halophila decipiens. Cymodocea rotundata mendominasi dikedua lokasi dengan kerapatan mencapai 1030 ind/m2. Nilai biomassa dibawah substrat (554,54 gbk/m2) lebih besar dibandingkan nilai biomassa diatas substrat (342,72 gbk/m2) diikuti nilai kandungan karbon dibawah substrat (193,31 gC/m2) yang lebih besar dibandingkan nilai kandungan karbon diatas substrat (119,99 gC/m2). Total kandungan karbon pada lokasi Pulau Menjangan Kecil adalah 32,18 ton karbon/ha dan Pulau Sintok adalah 4,18 ton karbon/ha. Blue carbon issue has become worldwide attention, UNEP through the concept of 2009 which has been to include vegetation seagrass beds as an absorbent of carbon in the ocean. The absorption of carbon that is stored through sediment and tissue in seagrass beds in the form of biomass. Research conducted on the island of Menjangan Kecil and island Sintok, Karimunjawa is to look at the level density, covering and absorption of carbon which is in biomass tissue seagrass ( the root, rhizoma and leaves ). Cover of seagrass density was measured by sampling the field using transect method 1m x 1m squares, identification of types of seagrass guidance from seagrasswatch book. Relationships density, biomass is made to see the value of the carbon content in the seagrass. Sampling density, seagrass cover and biomass values performed on all points, while carbon analysis on ashing method performed at the point of 50 m which is then converted to the value of biomass at another point. The results of the present study found 8 species of seagrasses that Enhalus acoroides, Thallasia hemprichii, Cymodocea rotundata, Cymodocea serrulata, Halodule uninervis, Halophila ovalis, Halophila minor, and Halophila decipiens. Cymodocea rotundata dominate in both locations with densities reaching 1030 ind/m2. Value biomass below the substrate (554.54 gbk/m2) indicates a value greater than the value of the biomass above the substrate (342.72 gbk/m2) followed by the value of the carbon content below the substrate (193.31 gC/m2) which is greater than the value carbon content above the substrate (119.99 gC/m2). Total carbon content in locations Menjangan Kecil Island is 32.18 tons of carbon and Sintok island was 4.18 tons of carbon.

Co-Authors Aan Pratama Abdul Ghofir Abdul Hadi Abdul Latif Mahakar Abdul Muis Prasetia, Abdul Muis Abdullah Afif Abdullah Afif Abidin Nur II Achmad Muhajir Adi Santoso Adiyoga, Diaz Agus Sabdono Agus Trianto Agustiningsih, Luluk Akbarinissa, Rr. Dyah Artati Akhmad Syarifudin Aklif Reza Muttaqin Aldion Adin Nugroho Ali Djunaedi Ali Djunaedi Ali Junaedi Ali Ridlo Altysia Putriany Amalia Rofiah Amalia Rofiah Amar, Fahri Ambariyanto , Ambariyanto Ambariyanto Anak Agung Istri Sri Wiadnyani Anam, Aufa Anastasia Dian R Anindya Wirasatriya Antonius Budi Susanto Apriliani, Seka Indah Ardhan Khanza Adhirajasa Ariel E San Jose Arif Rahman Arumning T. Fauziah Aufa Anam Azizah T.N., Ria Bastiar, Yusuf Bifa Aulia Manuhuwa Bintang Septiarani Broto Wisnu RTD Broto, R. T.D Wisnu Candhika Yusuf Cantik Sitta Devayani Cantika Elistyowati Andanar CB. Ronaldi Chandra E Chrisna Adhi Suryono Chrisna Adi Suryono Cristiana Manullang David Nugroho Delianis Pringgenies Delianis Pringgenies Denny Nugroho Sugianto Dharma, Laga Adhi Diah Permata Wijayanti Diaz Adiyoga Dina Ayu Magfirani Dinda Richa Kumarahaqi Donna Nur'Aurelya Mahardhika Dwi Haryanti, Dwi Dwi Saniscara Wati Edi Supriyo Edi Wibowo Edi Wibowo Kushartono Edi Wibowo Kushartono Edy Supriyo Edy Supriyo Edy Supriyo Edy Wibowo Edy Wibowo Elis Indrayanti Endika Meirawati Erik Wijaya Kusuma Ervia Yudiati Esti Rudiana Ety Parwati Ety Parwati Ety Parwati Fitriyan, Jodhi Kusumayudha Frijona Fabiola Lokollo Galih Arum Puspitaningtyas Aji Pangastuti Gangsar Bayu Setia Nugroho Gangsar Bayu Setia Nugroho Gazali Salim Ginzel, Fanny Iriany Gunawan Widi Santosa H. Endrawati Hadi Endrawati Hadi Endrawati Haniâ€™atun Nurfajriyah Hariyadi Hariyadi Harmoko Harmoko Haryo Farras Raditya Hutama Hawa, Adnin Kamil Bani Heri Yanti Hermawan Hermawan Hermawan Hermawan Hermin Pancasakti Kusumaningrum Hilal M I Wayan Warmada Ibnu Pratikto Ibnu Pratikto Ika Desie Wulandari Ira Kolaya Ira Kolaya, Ira Ireng Sigit Atmanto Irwani Irwani Isai Yusidarta Isti Pudjihastuti Ita Riniatsih Ita Widowati Itsna Yuni H Jelita Rahma Hidayati Johannes Hutabarat Jufri Ubay Julian Ransangan Jumawan Jumawan Jussac Maulana Masjhoer Justin Cullen Jusup Suprijanto Jusup Suprijanto Jusup Suprijanto Käll, Sofia Ken Suwartimah Ken Suwartimah Khairunnisa Khairunnisa Khoerunnisa, Rizka Nabila Kholilah, Nenik Koen Praseno Koen Praseno Kusuma, Erik Wijaya Laras Kinanti Pinandita Lilik Maslukah M. Andry Herdiatma M. Andry Herdiatma Madhu Pinastika Puji Lestari Madhu Pinastika Puji Lestari, Madhu Pinastika Puji Magfirani, Dina Ayu Mahakar, Abdul Latif Mahfud Mahfud Manuhuwa, Bifa Aulia Mas’ad Arif Mayestika, Pingkan Meitri Bella Puspa Mostafa Imhmed Ighwerb Muchammad Miftahul Ulum Muchammad Miftahul Ulum Muhammad Faiz Abadi Muhammad Rafi Tsaqif Muhammad Yusuf Muhammad Zainuri Muhammad Zainuri Muhammad Zainuri Muhammad Zainuri Muji Wasis Indriyawan, Muji Wasis Mujiyanto Mujiyanto Mustalafin Mustalafin Mu’alimah Hudatwi Nando Arta Gusti Pamungkas Narendra Prasidya Wishnu Natasya Erdza Aulia Ningrum, Malinda Satya Nirwani Soenardjo Njurumana, Steven Nggiku Norma Afiati Nugroho, Suciadi Catur Nur Taufik SPJ Nur Taufiq Nur Taufiq-Spj Orisu, Anthon Apolos Parameswari Iccha Nirmalabuddhi Wishnuputri Petta, Constantein Philipus Uli Basa Hutabarat Pingkan Mayestika Pingkan Mayestika Pradina Purwati Pradina Purwati Pratama, Candrika Primaswatantri Permata Putri Novianingrum, Milka Putri, Dhiya Aflah Luswanto Putri, Ni Putu Purba Nava Vidyadhari Putriningtias, Andika Raden Ario Rafsanjani A. Karim Rafsanjani A. Karim Raka Pramulo Sophianto Ramadhani, Muhammad Rizqi Reny Yesiana Retno W. Astuti Ria Azizah Ria Azizah Ria Azizah Ria Azizah Ria Azizah Tri Nuraini Rizky Imtihan R. Rizky Imtihan R., Rizky Imtihan Robertus Triaji Mahendrajaya Robertus Triaji Mahendrajaya Robertus Triaji Mahendrajaya Robin Robin Rohmah, Ivatur Rotua Malau Rozi Rr. Dyah Artati Akbarinissa Rudhi Pribadi Rudi Pribadi Sarah Nabilla Sari Budi Moria Sartika, Linda Selvi Marcellia Septiani, Nur Alifah Seto Haryoardyantoro Sitti Hartinah DS Sophianto, Raka Pramulo Sri Redjeki Sri Redjeki Sri Redjeki Sri Sedjati Sri Yulina Wulandari Subagiyo Subagiyo Subagiyo Subagiyo Sugiyanto Sugiyanto Sugiyanto Sugiyanto, Nenden Rose Suryono Suryono Sutrisno Anggoro Tarigan, Ariyo Imanuel Taufiq-Spj, Nur Tiara Nur Baeti Baeti Tri Karyawati Tria Nidya Pratiwi Ubay, Jufri Ulfah Nurjanah Ulfah Nurjanah Ulfah Nurjanah, Ulfah Umi Fatimah Valentina R Iriani Valentina R. Iriani W.L. Saputra Wahyu Adi Wahyudi, Yudisthio Wibowo, Muhammad Reyhan Widianingsih Widianingsih Widianingsih Widianingsih Widianingsih Widianingsih, - Wilis Ari Setyati Wilis Ari Setyati Wishnuputri, Parameswari Iccha Nirmalabuddhi Wita Kristianty Sirait Yasir, Moh. Yoki Ristadi Yoko Nozawa Yosi Yananda Sijabat Yudho Prasetyo Yunita Anggarini, Yunita Zulfiandi Zulfiandi Zulfiandi Zulfiandi

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