Garuda - Garba Rujukan Digital

Molecular Detection of Hemagglutinin Gene Fragment of Avian Influenza Virus H9 Subtype Obtained from Poultry Commercial Farm with Prominent Symptom of Decreased Egg Production Niken Respati Maharani; Heru Susetya; Michael Haryadi Wibowo
HAYATI Journal of Biosciences Vol. 29 No. 4 (2022): July 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.4.531-539

Avian influenza virus H9N2 subtype is considered a low pathogenic strain that has been reported in Indonesia since late 2016. The outbreak has caused economic losses for farmers due to the sharp drop in egg production. The evidence of the existence of AIV H9N2 has been published, but very limited information on the prominent symptom and macroscopic lesion. This research was a retrospective study of suspected avian influenza H9 subtype, obtained from layer commercial farm with recorded characteristic symptoms. Specific trachea samples were collected and further processed to be isolated, propagated using embryonated chicken egg, and then extracted the RNA for molecular detection using real-time reverse transcriptase-polymerase chain reaction (RT-qPCR). A positive result was further detected in its H-9 gene with RT-PCR technique and sequencing methods. One of five samples showed positive for RT-qPCR with CT value 30.19. Sequence analysis confirmed that the sample with characteristic macroscopic lesion could be detected in the presence of the AIV H9 subtype. Phylogenetic tree analyses revealed that this virus belongs to the China-Vietnam- Indonesia (CVI) lineage.

PREVALENSI KOLIBASILOSIS PADA AYAM BROILER YANG DIINFEKSI Escherichia coli DENGAN PEMBERIAN BIOADITIF, PROBIOTIK, DAN ANTIBIOTIK Ade Erma Suryani; Mohammad Faiz Karimy; Lusty Istiqomah; Ahmad Sofyan; Hendra Herdian; Michael Haryadi Wibowo
Widyariset Vol 17, No 2 (2014): Widyariset
Publisher : Pusbindiklat - LIPI

The purpose of this research was to study the efficacy of bio-additive (a mixture of Lumbricus rubellus meal extract, Morinda citrifolia leaf extract and lactic acid bacteria ), probiotics, and antibiotics on the prevalence co- libacillosis and healthy status of broiler that infected by E. coli strain Avian Pathogenic Escherichia coli (APEC). A total of 140 DOC were distribute randomly into 20 units of cage, each filled with 7 chickens were arranged in a completely randomized desig . Twenty cages were divided into 5 group , each treatment consisted of 4 replicates. The treatment group consisted of treatment A = infection of E. coli positive control), B = infection of E. coli + bio- additiv , C = infection of E. coli + probiotic , D = infection of E. coli + antibiotic , E = no E. coli infection negative contro). Feed base on corn- soybean is formulated as a basal fee . The experiments were conducted for 35 days, on day 21 chickens infected E. coli. ND vaccination is given at the age of four days and 15 days. The observed parameters were changes of macroscopic, isolation and identification of E. coli, changes in histopathology, blood profiles and antibody titer against ND. Results showed the prevalence colibasillosis on treatment B resulted in the lowest rate (33.3), results in the isolation and identification of chicken with positive clinical symptoms kolibasilosis infected APEC, and microscopic observations showed histopathological changes in the organs pancreas, heart, liver, and exchanges fabrisius lung. The results of the blood profile analysis showed the presence of the body’s defense mechanism against bacterial infectio , which is evident from the number of leukocytes in treatment A and C are higher tha treatment E (P> 0.0 ), red cell count treatment D higher than E treatment (P> 0.0 ), and total of Hb treatment A higher than treatment E P> 0.0 ). Based on the overall health status, it can be concluded that the administration of bioaditif decrease the prevalence o colibasillosis 67.7 % .

PREVALENSI KOLIBASILOSIS PADA AYAM BROILER YANG DIINFEKSI Escherichia coli DENGAN PEMBERIAN BIOADITIF, PROBIOTIK, DAN ANTIBIOTIK Ade Erma Suryani; Mohammad Faiz Karimy; Lusty Istiqomah; Ahmad Sofyan; Hendra Herdian; Michael Haryadi Wibowo
Widyariset Vol 17, No 2 (2014): Widyariset
Publisher : Pusbindiklat - LIPI

The purpose of this research was to study the efficacy of bio-additive (a mixture of Lumbricus rubellus meal extract, Morinda citrifolia leaf extract and lactic acid bacteria ), probiotics, and antibiotics on the prevalence co- libacillosis and healthy status of broiler that infected by E. coli strain Avian Pathogenic Escherichia coli (APEC). A total of 140 DOC were distribute randomly into 20 units of cage, each filled with 7 chickens were arranged in a completely randomized desig . Twenty cages were divided into 5 group , each treatment consisted of 4 replicates. The treatment group consisted of treatment A = infection of E. coli positive control), B = infection of E. coli + bio- additiv , C = infection of E. coli + probiotic , D = infection of E. coli + antibiotic , E = no E. coli infection negative contro). Feed base on corn- soybean is formulated as a basal fee . The experiments were conducted for 35 days, on day 21 chickens infected E. coli. ND vaccination is given at the age of four days and 15 days. The observed parameters were changes of macroscopic, isolation and identification of E. coli, changes in histopathology, blood profiles and antibody titer against ND. Results showed the prevalence colibasillosis on treatment B resulted in the lowest rate (33.3), results in the isolation and identification of chicken with positive clinical symptoms kolibasilosis infected APEC, and microscopic observations showed histopathological changes in the organs pancreas, heart, liver, and exchanges fabrisius lung. The results of the blood profile analysis showed the presence of the body’s defense mechanism against bacterial infectio , which is evident from the number of leukocytes in treatment A and C are higher tha treatment E (P> 0.0 ), red cell count treatment D higher than E treatment (P> 0.0 ), and total of Hb treatment A higher than treatment E P> 0.0 ). Based on the overall health status, it can be concluded that the administration of bioaditif decrease the prevalence o colibasillosis 67.7 % .

Molecular Characterization and Pathogenesis of Newcastle Disease Virus Isolated from Brontok Eagle in West Java, Indonesia Liza Angeliya; Yuli Purwandari Kristianingrum; Widya Asmara; Michael Haryadi Wibowo
HAYATI Journal of Biosciences Vol. 29 No. 5 (2022): September 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.5.648-657

Newcastle disease (ND) is a contagious disease in poultry and numerous birds of various ages. Eagle is considered a potential reservoir for ND transmission as a wild bird. This research was conducted to molecularly characterize Newcastle disease virus (NDV) isolated from ND cases in Brontok Eagle and analyze the pathogenesis in chicken embryos. qRT-PCR was conducted as conﬁrmation of NDV without mixing Avian Inﬂuenza (AI). Sequencing the fusion (F) and haemagglutinin-neuraminidase (HN) genes from the three NDVs was performed with a speciﬁc primer. Amino acid sequence compared with other NDV from Genbank. Pathogenicity, genetic variation, distance, and phylogenetic studies were analyzed using bioinformatics software (MEGA-X). This study analyzed pathogenesis based on lesions and distribution of viral antigens in chicken embryos infected with NDV. Observations were based on tissue lesions with HE and IHC staining. NDV isolated from three Brontok Eagles is classiﬁed as velogenic strain, virulent NDV (KRQKRF), and belonging to Genotype VII subgenotype VII.2. The NDV was detected in various organ lesions, more severe in the pulmo, trachea, proventriculus, and intestine of chicken embryos. That is still similar to the previous case reports in the ﬁeld. These results show that NDV, which infected Brontok Eagle, has similar molecular characteristics and pathogenesis in chickens. These cases could be a threat to the poultry industry. Further research, surveillance, and monitoring of wild birds are needed to obtain more NDV epidemiological information in wild birds.

Karakterisasi Molekuler dan Biologis Virus Fowl Aviadenovirus yang Diisolasi dari Peternakan Ayam Komersial Aditya Ahkami Pratomo; Ifah Khairunnizak; Arini Nurhandayani; Michael Haryadi Wibowo
Jurnal Veteriner Vol 23 No 3 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.3.306

Fowl Adenovirus (FAdV) adalah virus penyebab penyakit unggas dengan lesi karakteristik benda inklusi yang ditemukan pada lesi organ, terutama organ hati. Penyakit tersebut menyebabkan kerugian ekonomi, berupa kematian ayam dan efek imunosupresi. Sejak tahun 2018, wabah FAdV atau dikenal sebagai penyakit inclusion body hepatitis (IBH) dilaporkan terjadi pada peternakan ayam broiler, di beberapa provinsi di Indonesia. Penelitian ini bertujuan untuk melakukan isolasi, karakterisasi molekuler dan biologis pada telur ayam berembrio dan kultur sel hati embrio ayam. Sampel hati, bursa fabricius, dan limpa diperoleh dari 23 peternakan ayam komersial di Indonesia, yang terdiagnosis penyakit IBH sejak tahun 2018-2020. Deteksi molekuler dilakukan dengan teknik polymerase chain reaction (PCR), menggunakan primer spesifik gen hexon. Hasil amplifikasi positif, selanjutnya dilakukan sekuensing. Isolasi dilakukan dengan menggunakan telur ayam berembrio (TAB) specific pathogen free (SPF) dan kultur sel hati embrio ayam. Deteksi dan karakterisasi molekuler menunjukkan bahwa semua FAdV yang diisolasi dari peternakan unggas yang diperoleh dari kasus terdiagnosa penyakit IBH, dapat dikonfirmasi penyebabnya sebagai FAdV genotipe E, yang termasuk strain FAdV-8b. Isolasi pada TAB menunjukkan lesi perdarahan embrio dan pembengkakan pada organ hati. Propagasi isolat FADV tersebut menggunakan kultur sel hati embrio ayam, menunjukkan lesi sitopatik yang telah dapat teramati pada pasase pertama, berupa pembengkakan dan pembulatan sel yang terinfeksi. Wabah penyakit IBH pada ayam komersial dalam penelitian ini, dikonfirmasi penyebabnya adalah FAdV genotipe E, yang termasuk dalam serotipe 8b. Virus FAdV yang diisolasi pada TAB SPF menunjukkan lesi perdarahan embrio dan hepatitis. Virus FAdV yang diisolasi pada penelitian ini dinilai patogen pada embrio ayam SPF.

Deteksi Virus Newcastle Disease pada Burung Merpati (Columba Livia) dan Burung Tekukur (Streptopilia Chinensis) yang Menunjukkan Gejala Syaraf Nyoman Reishita Andriyani; Arfian Rahma Shanti; Liza Angeliya; Marla Anggita; Tri Untari; Agnesia Endang Tri Hastuti Wahyuni; Widya Asmara; Michael Haryadi Wibowo
Acta VETERINARIA Indonesiana Vol. 10 No. 3 (2022): November 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.3.220-227

Dewasa ini dilaporkan banyak kasus pada burung merpati (Columba Livia) dan burung tekukur (Streptopilia Chinensis) menunjukkan gejala syaraf, terutama: tortikolis, dan kepala gemetar, yang merupakan indikasi penyakit Newcastle Disease (ND). Kedua spesies burung tersebut banyak berkeliaran di lokasi farm ayam komersial untuk mencari pakan. Kondisi tersebut dapat bertindak sebagai faktor penular penyakit ND. Penelitian ini bertujuan mendeteksi virus ND pada burung merpati dan burung tekukur yang memperlihatkan gejala saraf, dengan uji serologis dan molekuler. Sampel darah diambil dari vena brakhialis, diproses untuk mendapatkan serum darah. Serum tersebut selanjutnya diuji dengan uji hemaglutinasi inhibisi (HI), untuk mendeteksi titer antibodi ND. Sampel pool otak, trakea, dan lien diekstraksi RNA-nya dan diamplifikasi dengan primer spesifik gen F virus ND. Sampel pool tersebut juga dikultur pada telur ayam berembrio specific pathogen free (TAB-SPF). Identifikasi dilakukan dengan uji hemaglutinasi (HA) dan HI dengan serum kontrol positif ND. Hasil deteksi serologis 7 sampel burung merpati menunjukkan titer antibodi ND bervariasi dari titer 25 sampai 28., sedangkan 2 sampel diperoleh seronegatif dengan titer 20. Salah satu pool gerusan organ kode M3/Sleman/2021 menunjukkan hasil RT-PCR positif. Analisis sekuens isolat virus ND tersebut termasuk NDV virulen dan dikelompokkan ke dalam genotipe VII-i. Pasase pool sampel organ tersebut masing-masing dikultur pada TAB-SPF dengan pasase sebanyak 3 kali, menunjukkan hasil negatif.

Characterization of Newcastle Disease Virus Isolated from Peacocks in Palembang City, South Sumatra Liza Angeliya; Akbar Agus Anshori Mussama; Syarifah Alawiyah; Tri Guntoro; Eko Agus Srihanto; Yuli Purwandari Kristianingrum; Widya Asmara; Michael Haryadi Wibowo
Jurnal Sain Veteriner Vol 41, No 1 (2023): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.76226

Introduction: Newcastle Disease (ND) is an infectious disease in various types of poultry caused by the Newcastle Disease Virus (NDV). Cases of ND in Indonesia have been reported in commercial and backyard chickens, pigeons, ducks and geese, even in eagles and peacocks. Peacock is a wild bird protected by Indonesia's Government Regulation No. 7 of 1999. This study aims to identify, isolate and characterize the NDV molecularly in cases diagnosed as ND in peacocks. Method: Samples were obtained from organs (lungs and spleen) of peacocks which showed neurological symptoms, diarrhoea and then died. Real-time RT-PCR ND was used to identify the cause of death of the peacock. Virus isolation and observation of embryonic changes and death were carried out on embryonic chicken eggs. Sequencing was carried out to characterize the F and HN entire genes of the NDV. The nucleotide sequences were analyzed using MEGA-X software, including amino acid prediction, analysis of genetic variation at the amino acid level, homology and construction of the phylogenic tree. Result: The results of the sample identification were positive for the Newcastle disease virus. Observations of chicken embryos are stunted, have few feathers, are haemorrhagic, and die in less than 60 hours. Virus isolation was obtained with a titer of 26. Molecular analysis showed that the RRQKRF cleavage site pattern in the F gene had homology of 95.8-97.6% and was in the same branching area as the previous ND virus in Indonesia. There were no amino acid mutations at the antigenic site, glycolysis and neutralization epitopes in the HN gene. Conclusion: The virus isolated from the peacock is a velogenic strain of NDV, subgenotype VII.2 and has a close genetic range to the NDV that has been previously reported in commercial and domestic poultry. This result shows that ND is also a threat to protected wild birds.

EVALUASI STATUS VIRULENSI ISOLAT Bacillus anthracis ASALNUSA TENGGARA DAN PAPUA MENGGUNAKAN METODE POLYMERASE CHAIN REACTION MULTIPLEX Maxs Urias Ebenhaizar Sanam; Widya Asmara; Agnesia Endang Tri Hastuti Wahyuni; Michael Haryadi Wibowo; Rahmat Setya Adji
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengevaluasi status virulensi 22 isolat Bacillus anthracis (B. anthracis) asal Nusa Tenggara dan Papua menggunakan metode polymerase chain reaction (PCR) multiplex dengan dua pasang primer nukleotida yang memiliki target amplifikasi gen spesifik pada kedua plasmid. Ektraksi DNA dilakukan dengan metode lisis panas. Pasangan primer PA5 dan PA8 digunakan untuk mengamplifikasi gen pagA pada pXO1, sedangkan pasangan primer 1234 F dan 1301 R mengamplifikasi gen capABC pada pXO2. Hasil reaksi PCR menghasilkan dua pita DNA berukuran sekitar 600 dan 800 bp pada 20 isolat. Namun, dua isolat lain, masing-masing hanya memiliki salah satu dari kedua ukuran pita DNA tersebut. Sebagian besar koleksi isolat asal Nusa Tenggara dan Papua (91%) masih memiliki kedua plasmid secara lengkap (pXO1+/2+) dan karena itu bersifat virulen, sedangkan dua isolat lain (9%) telah kehilangan salah satu plasmid virulennya sehingga bersifat avirulen. Disimpulkan bahwa PCR multiplex dengan dua pasang primer dengan target amplifikasi pada plasmid dapat digunakan untuk evaluasi status virulensi isolat B. anthraci.

EVALUASI KIT DETEKSI CEPAT TERHADAP SAMPEL OTAK ANJING TERINFEKSI VIRUS RABIES Michael Haryadi Wibowo; Tri Untari; Sidna Artanto; Surya Amanu; AETH. Wahyuni; Widya Asmara
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui potensi kit deteksi cepat Anigen® rapid test kit rabies Ag dalam mendeteksi virus rabies pada sampel otakanjing yang diperoleh dari lapangan yang meliputi batas deteksi, kecepatan reaksi, uji reaksi silang, uji sensitivitas, dan spesifisitas. Batas deteksi ditentukan dengan pengenceran secara serial kontrol positif virus rabies dan selanjutnya diuji dengan rapid test kit sesuai petunjuk produsen. Uji reaksi silang dilakukan dengan canine parvovirus, Escherichia coli, dan Salmonella sp. Uji sensitivitas dan spesifisitas dilakukan terhadap sampel otak yang telah dikonfirmasi positif rabies dengan uji fluorescent antibody technique. Konfirmasi uji rapid test tersebut dilakukan dengan reverse transcriptase polymerase chain reaction. Berdasarkan data yang diperoleh dalam penelitian ini dapat disimpulkan bahwa Anigen® rapid test kit rabies Ag mampu mendeteksi sampel yang mengandung virus rabies dengan titer 0,5 x log 106,5/0,03 ml, dengan rata-rata kecepatan reaksi 1,8 menit 29,35 detik (kurang dari 2 menit). Di samping itu Anigen® rapid test kit rabies menunjukkan tidak terdapat reaksi silang dengan canine parvovirus, Escherichia coli, dan Salmonella sp. serta mempunyai sensitivitas 92,30% dan spesifisitas 97,90%

IDENTIFIKASI Escherichia coli O157:H7 DARI FESES AYAM DAN UJI PROFIL HEMOLISISNYA PADA MEDIA AGAR DARAH I Wayan Suardana; Iwan Harjono Utama; Michael Haryadi Wibowo
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan melakukan isolasi dan identifikasi serotipe lokal Escherichia coli (E. coli) O157:H7 dan uji profil hemolisisnya pada media agar darah. Isolasi bakteri dilakukan dengan media eosin methylene blue agar (EMBA), dilanjutkan dengan identifikasi pada media selektif sorbitol MacConkey agar (SMAC) dan uji konfirmasi menggunakan uji aglutinasi lateks O157 serta uji antiserum H7 sebagai konfirmasi akhir dari E. coli O157:H7. Gambaran hemolisis diuji dengan menumbuhkan isolat pada media agar darah domba. Hasil penelitian menunjukkan bahwa 7 isolat (8,54%) dari 82 sampel feses ayam teridentifikasi E. coli O157:H7 dan memperlihatkan profil enterohemolisis seperti halnya isolat kontrol ATCC 43894. Dari hasil penelitian disimpulkan bahwa isolat lokal E. coli O157:H7 hasil isolasi dari feses ayam diketahui memiliki patogenitas yang tinggi terkait dengan dihasilkannya enterohemolisin yang merupakan marka/penanda kemampuan dari isolat untuk menghasilkan faktor virulensi Shiga like toxin.

Title

Found 51 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 51 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 51 Documents
Search