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Green Synthesis of Silver Nanoparticles Using Caulerpa racemosa Extract as Bioreductor: Characterization and Antibacterial Activity Barleany, Dhena Ria; Selviroh, Selviroh; Saleh, Afiatta Ilhan; Pitaloka, Alia Badra; Gunawan, Akbar
Jurnal Rekayasa Kimia & Lingkungan Vol 20, No 2 (2025): Jurnal Rekayasa Kimia & Lingkungan (December, 2025) In Press
Publisher : Chemical Engineering Department, Syiah Kuala University, Banda Aceh, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23955/rkl.v20i2.45873

Abstract

Silver nanoparticles (AgNPs) have attracted much attention due to their broad antimicrobial properties and have found many applications in various fields. In the current study, AgNPs were synthesized using an environmentally friendly method by utilizing Caulerpa racemosa extract as a bioreduction agent. This study aims to investigate the formation of AgNPs mediated by Caulerpa racemosa extract and to observe their antimicrobial capability. The concentration of silver nitrate (AgNO3) was varied to investigate its influence on the shape, size, and uniformity of the silver particles formed. The Fourier Transform Infra-red (FT-IR) test results revealed that Caulerpa racemosa extract has an (-OH) functional group that reduces Ag+ ions to Ag0, making it effective as a bioreductor for AgNPs synthesis. The shape and size of AgNPs were examined using Scanning Electron Microscope (SEM), and the results confirmed that increasing AgNO concentration from 5 to 20 mM produced larger and agglomerated particles. Additionally, AgNPs inhibitory effect on the growth of pathogenic Escherechia coli (gram-negative bacteria) was evaluated using the well diffusion method. The findings showed that AgNPs improved the antibacterial properties when incorporated into chitosan-based film solution. This indicates that AgNPs exhibit strong antibacterial activity against gram-negative bacteria and hold promises for use in bio-related applications, including food packaging and biomedical uses
Co-Authors Ade Irman Ade Irman Mutakin Ade Irman Saeful Mutaqin Ade Irman SM Ade Irman, Ade Alia Badra Pitaloka, Alia Badra Alifia Puspa Septianingrum Amelia Rachmawati Ananta, Surya Atmadilaga Ani Umyati Ani Umyati Arini Syafira Utami Asep Ridwan Asih Machfuzhoh Atia Sonda Bobby Kurniawan Budi Darma Maulana Cindy Christine Miranda Damasa G Desmiani Denni Kartika Sari Dewayana, Triwulandari Satitidjati Dhena R. Barleany Dhena Ria Barleany Dina Estining Tyas Lufianawati Dyah Lintang Trenggonowati Ekana Kusumaningrum Enny Fitriyanti Erien Amelia Erlin Filiandini Evi Febianti Evi Febianti Fajri Humaedi Fani Rahma Damayanti Fellek Getu Tadesse Galih, Restu Azi Hadi Setiawan Hadi Setiawan Hadi Setiawank Hafair Mubarak Ibrahim Nur Farabi Intan Nauroh Iqbal Iqbal Iveline Anne Marie Kadarsah Suryadi Kee Evelyne Khairiyyah Rusydi khatili, putiri buana kulsum kulsum Kulsum M.T. M.Naufal Muadz Mentari Indria Cahya Merdiana Dewantari, Nustin Muhamad Ridwan Muharni, Yusraini Mukti Lestari Mutakin, ade irman Nuraida Wahyuni Nurul Ummi Nustin Merdiana Dewantari Purbasari, Intan Puti Nur Hardiyanti Putiri B. Katili Putiri Bhuana Katili Putiri Buana Khatili Putro Ferro Ferdinant Putro Ferro Ferdinant Ratna Ekawati Restu Wigati, Restu Retno Sulistyo Dhamar Lestari Rettrikar Kiswindyatmoko Ria Barleany, Dhena Rifan Maulana Rizki Akbari Robi Setiawan Saeful Mutaqin S., Ade Irman Saleh, Afiatta Ilhan Selviroh, Selviroh Shera Dwi Andini Sirajuddin Sirajuddin Sirajuddin Sirajuddin Syahputra, Galang ulfah, Maria Ummi, Nurul Utami Damayanti Vidya Nursekha Wahyu Susihono Yanuar Sutanto Zainal Abidin