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All Journal ACTA VETERINARIA INDONESIANA Jurnal Veteriner Jurnal Sains Peternakan Jurnal Sain Peternakan Indonesia Jurnal Peternakan Indonesia Jurnal Ternak Tropika Livestock and Animal Research JURNAL ILMIAH PETERNAKAN TERPADU Jurnal Peternakan Nusantara Jurnal Ilmu Peternakan Terapan Wahana Peternakan Jurnal Nukleus Peternakan COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat Jurnal Peternakan Lahan Kering Jurnal Sosial dan Sains Jurnal Kedokteran Hewan Lentera: Multidisciplinary Studies Journal of Livestock and Animal Health (JLAH) Animal Agricultura Journal of Innovative and Creativity Lentera: Multidisciplinary Studies
Thomas Mata Hine
Fakultas Peternakan, Kelautan Dan Perikanan, Universitas Nusa Cendana
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Journal : Jurnal Nukleus Peternakan

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PENGARUH PENGGUNAAN TIGA JENIS KUNING TELUR DALAM PENGENCER AIR KELAPA MUDA TERHADAP KUALITAS SEMEN SAPI BALI (Impact of Adding Three Different Egg Yolks to Young Coconut Water Diluent on Bali Cattle Semen Quality) Ulu, Krista Florida; Belli, Henderiana L.L; Riwu, Agustinus R.; Hine, Thomas M.
JURNAL NUKLEUS PETERNAKAN Vol 11 No 2 (2024): Desember
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v11i2.7797

Abstract

Young coconut water (CW) can be used as a diluent which can provide nutrients and other protective substances for the life of spermatozoa. Egg yolk is one of the important components in semen diluent, because it has a very important role in protecting the plasma membrane and acrosome during storage. The aim of this research was to determine the best type of egg yolk in combination with young coconut water to maintain liquid semen quality of Bali cattle. This research used a complete random design consisting of 6 treatments and 5 replications. The basal diluent in all treatments was 80 percent of CW combined with 20 percent chicken egg yolk (C), duck egg yolk (D), quail egg yolk (Q). The treatments in question are P0= CW-C, P1= CW-D, P2= CW-PQ, P3= CW-CD, P4= CW-CQ, and P5= CW-CDQ. Data were analyzed using one-way ANOVA and Duncan tests. Until the 6th day of storage, the P3 treatment produced higher semen quality than the control (P<0.05) except that the sperm abnormality variable showed no significant difference (P>0.05). On the other hand, treatment P3 showed no significant difference from treatments P1, P2, P4, and P5 (P>0.05). The quality of Bali cattle semen up to the sixth day of storage in treatment P3 was: 42.00%. 48.76%, 5.71%, 6.2 days respectively for the variables motility, viability, abnormalities and sperm survival. The results of this study concluded that a combination of chicken and duck egg yolks added to coconut water is the best diluent that can maintain the quality of Bali cattle sperm.
PENGARUH WAKTU PRA PEMBEKUAN TERHADAP KUALITAS SEMEN BEKU DALAM PENGENCER SPERMAX (The Effect of Pre-Freezing Time on The Quality of Frozen Semen of Landrace Boars in Spermax Diluent) Dui, Theresia A. A. T.; Nalley, Wilmientje M; Lawa, Alvrado B; Telupere, Franky M S; Hine, Thomas M; Setyani, Ni M P
JURNAL NUKLEUS PETERNAKAN Vol 12 No 1 (2025): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v12i1.22383

Abstract

This research aimed to investigate the effect of pre-freezing time on the quality of frozen Landrace boar semen. Semen was collected from four 2-3 year old Landrace boars. The semen was evaluated macroscopically and microscopically. Subsequently, good quality semen was diluted in a basic extender, then subjected to a holding time of 2 hours at 27-28°C. The semen was then centrifuged at 2000 rpm for 15 minutes. The spermatozoa pellet was re-diluted with a Spermax extender, packaged into 0.5 mL straws, and equilibrated for 2 hours at 3-5°C. After equilibration, the straws were frozen by placing them 10 cm above the surface of liquid nitrogen at ±-110°C for durations of 3, 6, 9, and 12 minutes. The straws were then immersed into liquid nitrogen at -196°C. Thawing was performed by immersing the straws into a water bath containing water at 37°C for 30 seconds, followed by evaluation of semen quality. The results showed that pre-freezing time had no significant effect (P>0.05) on semen quality after dilution and equilibration. However, it had a significant effect (P<0.01) on the recovery rate, motility, and viability of spermatozoa after thawing, with the best treatment being 9 minutes. It was concluded that a pre-freezing time of 9 minutes resulted in the highest quality of frozen Landrace boar semen compared to other treatments.
EVALUASI KUALITAS SEMEN BEKU BABI LANDRACE DALAM PENGENCER SPERMAX MODIFIKASI DENGAN WAKTU PRE-FREEZING YANG BERBEDA (Assessment of Landrace Boar Frozen Semen Quality in Modified Spermax Diluent Under Varying Pre-freezing Durations) Kii, Mariana B.I.; Hine, Thomas M; Bette, Yustiany Y; Nalley, Wilmientje M
JURNAL NUKLEUS PETERNAKAN Vol 12 No 1 (2025): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v12i1.22385

Abstract

Research was conducted to investigate the impact of different pre-freezing durations (PF) using a modified spermax (M) extender on the quality of frozen landrace boar semen. The study utilized fresh semen from four healthy Landrace boars aged 2-3 years. Fresh semen meeting SNI standards was diluted in SM extender and held at room temperature (27-28℃) for two hours. Subsequently, the semen was centrifuged at 2000 rpm for 10 minutes, the supernatant was discarded, and the sperm pellet was re-diluted with SM extender at a 1:1 ratio. The semen was then packaged in 0.5 mL straws, placed on a freezing rack, and allowed to equilibrate for two hours at 3-5℃. Following equilibration, the semen was re-evaluated, and the straws were frozen above liquid nitrogen at -110℃ with varying pre-freezing times of 5, 8, 11, and 14 minutes. After the pre-freezing period, the straws were plunged into liquid nitrogen for final freezing and then stored in liquid nitrogen containers at -196℃. The results indicated that the duration of pre-freezing time did not lead to statistically significant differences (P>0,05) in sperm motility, viability, abnormality, and recovery rate (RR). In conclusion, pre-freezing times ranging from 5 to 14 minutes had a similar impact on the quality of frozen landrace boar semen.
PENGARUH LEVEL ZINK DALAM PENGENCER TRIS KUNING TELUR TERHADAP KUALITAS SEMEN CAIR SAPI ANGUS (The effect of zinc level in the tris egg yolk extender on the quality of angus liquid semen) Siena, Sofia M.S.; Hine, Thomas Mata; Nalley, W. Marlene; Marawali, Aloysius
JURNAL NUKLEUS PETERNAKAN Vol 11 No 1 (2024): Juni
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/nukleus.v11i1.8346

Abstract

Efforts to improve cattle productivity can be done by using artificial insemination (AI) technology. Many factors determine the success of AI, one of which is the diluent used in semen preservation. The purpose of this study was to determine.the effect.of zinc level in.tris-egg yolk.diluent (T-EY) on the.quality of liquid semen of Angus bull. Semen is collected once a week using.an artificial.vaginal method from a three-year-old Angus bull in an undisturbed or healthy body and reproductive organs. Semen was diluted with T-EY diluent which had been added with zinc at several levels, 0 mg control treatment (T0), 10.mg (T1), 20.mg (T2), 30.mg (T3), 40 mg (T4). The diluted semen was.stored at a temperature of 3-5oC, then observed every 24 hours for.motility, viability,.abnormalities and survival of.spermatozoa to a minimum motility of 40%. The.results showed.that the addition of 20 mg of zinc in the T-EY.diluent (T2) resulted in a higher.quality of liquid semen of Angus bull (P˂0.05) than other.treatments. The quality of the liquid semen (motility, viability, abnormalities and survival of spermatozoa) were motility 44±5.75%, viability 48.39±13.59%, abnormality 6.91±1.85% and durability.live 5.60±0.89 days. It was.concluded that the.addition.of 20 mg zinc in the.tris-egg yolk diluent produced liquid semen of Angus bull with higher quality than other treatments.
Co-Authors A. Kaka A. Parakkasi A. Parakkasi Abdullah Baharun Alfred Nubatonis Aloysius Marawali Aloysius Marawali Aloysius Marawali Aloysius Marawali Arief Boediono Ayang C. Tafuli Banamtuan, A. N. Bayu Rosadi Bette, Yustiany Y Bire Lawa, Alvrado Burhanuddin * Burhanuddin . C. Tafuli, Ayang D. J. Djawapatty Doda Dengi, Donatus Donatus Doda Dengi Dondin Sajuthi Dui, Theresia A. A. T. Enny T Setiatin Enny T Setiatin, Enny Franky M S Telupere George Valentino Kosat Grasele, Fransiskus Allianus Gue, Febronia Dhenge Gunawan G Gunawan G, Gunawan Henderiana Belli Henderiana L.L Belli Heri Armadianto Heri Armadianto Hilly, Fanny Tesalonika Honin, Olfi Mariana Iman Supriatna Ina Mato, Sefania J A Tamoes Jernih Blegur Johanis Ly Kihe, Johny Nada Kii, Mariana B.I. Kirenius Uly kirenius uly Kirenius Uly Kirenius Uly Kristina Meldiana Hayati Kurnia Wawang, Silviani L. L Belli, Henderiana L. L Belli, Henderiana Lawa, Alvrado B Mardemus Misa Maria E.E. Fernandez Maria Epifania Barek Maria Orsella Rindiani Mariana Nenobais Maritje Aleonor Hilakore Masae, Sinoptis Melkiur Nalley, Marlene Nalley, W. Marlene Nalley, Welmince M. Nalley, Wilmientje M Nalley, Wilmientje M. Nalley, Wilmientje Marlen Ni Made Paramita Setyani Nuba, Maria Deran Odo, Remigius Abha Pandahuki, Fransiska Onya Papituan, Mardan Laes Petrus Kune Petrus Kune Petrus Kune Petrus Kune Petrus Kune Riwu, Agustinus R. Sefania Ina Mato Selsiana Dongkot Setyani, Ni M P Siena, Sofia M.S. Sofia Marlize Soleman Manaze Weiri Sinda Stef Amiron Ratu Tapatab, Kelvin Alberto Imanuel Telupere, Franky M S Toto, Novaldy Imanuel Tree May Thiessen Outang Twen O. Dami Dato Ulu, Krista Florida Uly, Kirenius W. M. Nalley W. Marlene Nalley W. Marlene Nalley Wilmientje M. Nalley Wilmientje Marlen Nalley Wilmientje Marlene Mesang Nalley Wilmientje Marlene Nalley, Wilmientje Marlene Wilmintje Marlene Nalley Wirona Adu Yohanes Paulus Martinus Bay Yohanis Umbu Daku Waluwanja Yustiani Yuliana Bette Yustiany Yuliani Bette Yusuf Sae