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Journal : JOURNAL OF COASTAL DEVELOPMENT

DIET DEVELOPMENT FOR MUD CRAB (Scylla serrata) AQUACULTURE USING LOCAL MATERIAL AVAILABLE IN CENTRAL JAVA Johannes Hutabarat
JOURNAL OF COASTAL DEVELOPMENT Vol 2, No 2 (1999): Volume 2, Number 2, Year 1999
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Phase I study (1996/1997) indicated that the local protein materials at relatively low cost were found abundance in Central Java throught the year. Their nutritional levels i.e. protein content, amino acids profile and availability are suitable to be used for mud-crab feed development. It is therefore the selected protein materials such as : soybean, mysidaceae, trash fish and saga, are then subjected to the formulation of experimental diet for mud crab on-growing culture (Phase II study 1997/1998) The aims of study are to determine the nutritional performance of experimental diets (novel diets) for mud-crab scylla growth, food utilization and protein efficiency during the feeding trial. The study has been conducted in a 12 bamboo-cages of 1.0 x 0.5 x 0.70 m immersed in BW ponds at CZEL “Prof. Gatot Rahardjo J.S – UNDIP”, Jepara and the stocking density of wild mud-crab (50-60 gram) was 1 pieces /box (0.2 x 0.2 m). Experimental diet (pellet formed) consists of four treatments i.e. diet A (25% dietary protein level), diet B (30%),          diet C (35%) and diet D (40%). The growth and nutritional parameters observed as response to the experimental diets tested are : Individual growth and growth increment, Specific Growth Rate (SGR), FCR, NOU amd PER. The completely Randomize Design was adopted in this study and then followed by Duncans’s New Multiple Range Test in order to determine the difference among the treatments. The results derived from this study indicated that diet C (dietary protein level of 30%) appeared to be the best diet wheather for the growth (W and SGR0 or nutritional performance (FCR, PER, and NPU) and followed by diet B, D and A. Meanwhile, during the course of feeding trial water quality of experimental media remain in good condition to support ud-crab growth.
THE EFFECT OF DIFFERENT C:N AND C:P RATIO OF MEDIA ON THE CONTENT OF POLYHYDROXYBUTYRATE IN BIOFLOC INOCULATED WITH BACTERIUM Bacillus cereus Supono .; Johannes Hutabarat; Slamet Budi Prayitno; YS Darmanto
JOURNAL OF COASTAL DEVELOPMENT Vol 16, No 2 (2013): VOLUME 16, NUMBER 2, YEAR 2013
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Biofloc technology  has  added values in aquaculture management, both in water quality  management and feeding  management.  As an optional feed, biofloc is capable to enhance growth due to high protein content.  Bacteria, main component biofloc, can produce polyhydroxybutyrate (PHB) as reserve of energy and growth accelerator for fish. The aim of the research were to study the effect of the different C:N and C:P ratio of media on the content of polyhydroxybutyrate in biofloc and to determine optimum media to produce high polyhydroxybutyrate content in biofloc. The experiment was arranged in factorial  with completely randomized design  in  three replications.  Treatments were C:N ratio of 15, 20, 25 and C:P ratio of 75, 100, and 125. The result showed that C:N ratio and C:P ratio of media and their interaction  affect the content of polyhydroxybutyrate in biofloc.  C:N ratio of 20 and C:P ratio of 125 resulted in most polyhydroxybutyrate (29.25±7.376 mg g-1 biofloc dry weight). Ratio of  C:N  of media gave linier and quadratic responses and C:P ratio of media gave linier one. Optimum polyhydroxybutyrate production was obtained at C:N ratio of 20.9 and C:P ratio of  125 resulting in 29.66 mg  g-1 biofloc dry weight (2,97%)
EFFECT OF USING GUILLARD AND WALNE TECHNICAL CULTURE MEDIA ON GROWTH AND FATTY ACID PROFILES OF MICROALGAE Skeletonema sp. IN MASS CULTURE Vivi Endar; . Sarjito; Johannes Hutabarat; Budi Prayitno
JOURNAL OF COASTAL DEVELOPMENT Vol 16, No 1 (2012): Volume 16, Number 1, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Live food, especially microalgae Skelotenoma sp. is a key success factor in shrimp aquaculture. To that end, the provision of Skeletonema sp. mass with a high nutrient content is needed. Nutritional quality of microalgae depends on the culture media used. The purpose of this study was to investigate effect of the use of different technical culture medium (Walne and Guillard) on the growth, protein content and fatty acid profile in microalgae culture Skelotenoma sp. Skeletonema sp. obtained from the Laboratory of Natural Feed BBPBAP Jepara. Culture method used was a mass with two different media (modified Walne and technical Guillard), with 12 replications. Data analysis were analyzed by using T test, while the protein content analysis was performed by Kjedahl method.The fatty acids were determined by using in situ transesterification. The results showed that the growth of Skeletonema sp. was markedly different between media Walne and technical Guillard. Guillard medium revealed lag phase after 44 hours (observation to 6) with a cell density of 48.00 x 104 cells/ml, then entered the exponential phase at 48. (Observation to 7) with a cell density of 70.25 x 104 cells / ml, while the stationary phase occurred in after hours to 52 (observation to 8) with a cell density of 86.75 x 104 cells / ml and death phase began at the 56 ( observations to 9) with a cell density of 54.58 x 104 cells / ml. Growth of Skeletonema sp. cultured with culture medium technical Walne showed a similar pattern in the lag phase to 44 hours of observation (observation to 6 with the cell density is 117.17 x 104sel/ml, exponential phase and stationary phase were detected from hour to 48 (observation to 7) with a cell density is 160.83 x 104 cells / ml. Later phases of death from an hour to 52 (observation to 8) with a cell density of 122.25 x 104 cells / ml. then long culvation or Skeletonema sp stationary phase in Guillard media over a period of 4 hours than Walne medium. Total fatty acids of Skeletonema sp. cultured in Guillard medium resulted in higher yields.
STABILITY OF LOCAL RAW MATERIALS FOR MUD CRAB FEED DEVELOPMENT Johannes Hutabarat
JOURNAL OF COASTAL DEVELOPMENT Vol 1, No 1 (1997): Volume 1, Number 1, Year 1997
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

The aims of study were to identify the potency and nutritional values of local raw materials available in Central java, and to develop the mud crab feed using selected raw materials for its deffatening culture. The potency level of local raw materials was determined by using secondary data available in relevant technical institution e.g. Agriculture Department, Fisheries Bureau and followed by direct site survey in the production centre of agriculture and fisheries by catch in Central Java, i.e  Pekalongan, Kendal, Semarang, Jepara, Pati and Rembang. Data obtained were analyzed descriptively to determine the resource of the potentials of local raw materials and followed by determining their nutritional values, profile and availability of assential amino acids (EAA) and essential fatty acids (EFA), using standard method available. The results indicated that the local raw materials for protein sources are found in abundance in Central Java throughout the year with relatively cheap price i.e: trash fish, mysid, squid, blood meals, worm-meals and shrimp head-meals (animal protein) and saga, soybeans (plant protein). The protein level of selected raw materials were high (41.15% – 80.35%) and the highest level was found in blood meal and followed by squid, trash fish and shrimp head meals. The selected raw materials, generally contains 10 essential amino acids (Arginine, lysine, histidine, phenylalanine, leucine, isoleucine, methionine, valine, threonine, and trypthophane) and a long chain of EFA (n-3 HUFA) and (n-6 HUFA) which are required by mud crab for their growth. The selected local raw materials are therefore nutritionally suitable for mud crab feed development in Central Java.
Co-Authors - Masrurotun . Sarjito Abidin Nur II Andi Sagita Anindya Wirasatriya Arumning T. Fauziah Ayu Istiana Fiat Ayudya Wisma Hapsari Aziz Nur Bambang Bambang Argo Wibowo Bella Manik Hapsari Bob Suroso Bosma, Roel Bosma, Roel H. Budi Prayitno Caesa, Genio Cici Ulviyadipura Denny Nugroho Sugianto Diana Chilmawati Diana Chilmawati Diana Rachmawati Diana Rachmawati Dicky Harwanto Eko Nurcahya Dewi Eko Nurcahya Dewi Eko Nurcahya Dewi Eko Nurcahyo Dewi Elsyaday Widhi Cahyono, Elsyaday Widhi Endang Arini Endra Catur Pamungkas Ertris Bergas Taqwdasbriliani Ervia Yudiati Fadityas Desi Aniputri Falstiyan Syahendra, Falstiyan Fatchurochman, Vava Fhibia Jati Fitria Aditama Fritta Wijayanti Hadi Pranggono Haeruddin Haeruddin Hariyadi Hariyadi Hariyadi, Putut Herawati Vivi Endar Hernowo, Ilham Agung Ika Nurul Asriyanti Intan Suriyanti Istiyanto Samidjan Istiyanto Samidjan Ita Widowati Jelita Rahma Hidayati Kunarso Kunarso Lela Nurfitriani Lestari Lakhsmi Widowati Manullang, Corry Yanti Manullang, Corry Yanti Marlia Ulfa Puspitasari Mostafa Imhmed Ighwerb Muhammad Faiq Marwa Noercholis Muhammad Latif Usman Niken Dwi Prasetyarini Norma Afiati Nurmanita Rismaningsih Ocky Karna Radjasa Ocky Karnaradjasa Pane, Pranada Parichat Wetchayount Pinandoyo Pinandoyo Pinandoyo Pinandoyo Pramana, I Nengah Gunaya Pranada Pane Pratama, Arsy Latif Putut Har Riyadi Ratna Widiastuti Ratri, Kartika Sulistyaning Restiana Wisnu Ariyati Retno Hartati Rismaningsih, Nurmanita Ristiawan Agung Nugroho Ristiawan Agung Nugroho Rudhi Pribadi Samsul Rizal Sarjito - Schrama, Johan Seto Windarto Sipayung, Andy Situmeang, Ameria slamet budi prayitno Slamet Budi Prayitno Solly Aryza Sri Rejeki Sri Rejeki Sriwati Sriwati Steven Subandiyono Subandiyono Subekti, Mulat Suhendra Suminto Suminto Suminto Suminto Suminto Suminto Supono . Suradi Wijaya Saputra Suriyanti, Intan Susana E. Ratnawati Susanna Endah Ratnawati Sutrisno Anggoro Tita Elfitasari Titik Susilowati Tony Suhartono Tony Suhartono Tri W. Agustini Tri Winarni Agustini Tri Winarni Agustini Trienes, Yoni Trisnani Dwi Hapsari Tristiana Yunarti Vivi Endar VIVI ENDAR HERAWATI Vivi Endar Herawati Widianingsih Widianingsih Widodo S. Pranowo Widyawati Nurul Fajri Windarto, Seto YS Darmanto