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Journal : Scripta Biologica

APPLICATION OF PROTEASE ENZYMES IN PROTEIN UTILIZATION AT GROWTH OF WHITE SNAPPER FISH (Lates calcalifer) Fitria Aditama; Johannes Hutabarat; Subandiyono Subandiyono
Scripta Biologica Vol 5, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

White Kakap (Lates calcalifer) is a superior commodity and has a high nutritional value. Growth factors are hampered by the ineffectiveness of the digestive process. Protease is an enzyme capable of hydrolyzing peptide bonds in proteins. Feed is a factor that predominantly affects the growth of fish because feed serves as a supplier of energy to support growth. Protein digestion in aquatic animals requires proteases as a catalyst. Enzymes that play an important role in the process of protein digestion are proteases. The purpose of this study is to examine the effect of adding different protease enzymes to artificial feed on the efficiency of protein utilization in the growth of white snapper (Lates calcalifer). The results of this study are expected to provide information about the importance of protease enzyme application in artificial feed in increasing protein hydrolysis, so that protein can be easily utilized by the body of white snapper (Lates calcalifer). The cultivars tested were white snapper (Lates calcalifer). The cultivation comes from intensively cultivated Lampung with an average weight of 6.5 ± 0.44 g / tail with a length ranging from 4-5 cm, as many as 75 heads. The research method is experimental laboratories using Completely Randomized Design (RAL). The tested feed was in the form of artificial feed with protein content of 40% and different protease dosage. The tested treatment was A feed (0.0% Protease enzyme from artificial feed weight), B (0.05% Protease enzyme from artificial feed weight), C (0.1% Protease enzyme from artificial feed weight), D (0 , 15% Protease enzyme from artificial feed weight), E (0.2% Protease enzyme from artificial feed weight). The ingredients of white snapper fish feed consist of fish meal, corn flour, soybean meal, shrimp flour, fish oil, corn oil, CMC / wheat flour, minerals and vitamins. Research data obtained for 40 days using analysis of variance (anova). If there is a real effect on the treatment then Duncan multiple area test is done. The result of this research indicated that the giving of protease 0,2% in artificial feed with protein 40% able to produce feed consumed Kakap Putih 43,43 ± 0,13 g, FCR 0,35 ± 0,002, Feed Pureness equal to 67,9 ± 1.23%, Protein Digestibility 98.25 ± 0.03%, Protein Retention 68.8 ± 0.23%, PER 7.97 ± 0.04, Fat Retention 0.6 ± 0.005%, E / P 4, 6 cal / g, thus giving effect on Specific Growth (SGR) 6.032 ± 0.17% and Length 8.3 ± 0.2 cm.
Co-Authors - Masrurotun . Sarjito Abidin Nur II Andi Sagita Anindya Wirasatriya Arumning T. Fauziah Ayu Istiana Fiat Ayudya Wisma Hapsari Aziz Nur Bambang Bambang Argo Wibowo Bella Manik Hapsari Bob Suroso Bosma, Roel Bosma, Roel H. Budi Prayitno Caesa, Genio Cici Ulviyadipura Denny Nugroho Sugianto Diana Chilmawati Diana Chilmawati Diana Rachmawati Diana Rachmawati Dicky Harwanto Eko Nurcahya Dewi Eko Nurcahya Dewi Eko Nurcahya Dewi Eko Nurcahyo Dewi Elsyaday Widhi Cahyono, Elsyaday Widhi Endang Arini Endra Catur Pamungkas Ertris Bergas Taqwdasbriliani Ervia Yudiati Fadityas Desi Aniputri Falstiyan Syahendra, Falstiyan Fatchurochman, Vava Fhibia Jati Fitria Aditama Fritta Wijayanti Hadi Pranggono Haeruddin Haeruddin Hariyadi Hariyadi Hariyadi, Putut Herawati Vivi Endar Hernowo, Ilham Agung Ika Nurul Asriyanti Intan Suriyanti Istiyanto Samidjan Istiyanto Samidjan Ita Widowati Jelita Rahma Hidayati Kunarso Kunarso Lela Nurfitriani Lestari Lakhsmi Widowati Manullang, Corry Yanti Manullang, Corry Yanti Marlia Ulfa Puspitasari Mostafa Imhmed Ighwerb Muhammad Faiq Marwa Noercholis Muhammad Latif Usman Niken Dwi Prasetyarini Norma Afiati Nurmanita Rismaningsih Ocky Karna Radjasa Ocky Karnaradjasa Pane, Pranada Parichat Wetchayount Pinandoyo Pinandoyo Pinandoyo Pinandoyo Pramana, I Nengah Gunaya Pranada Pane Pratama, Arsy Latif Putut Har Riyadi Ratna Widiastuti Ratri, Kartika Sulistyaning Restiana Wisnu Ariyati Retno Hartati Rismaningsih, Nurmanita Ristiawan Agung Nugroho Ristiawan Agung Nugroho Rudhi Pribadi Samsul Rizal Sarjito - Schrama, Johan Seto Windarto Sipayung, Andy Situmeang, Ameria Slamet Budi Prayitno slamet budi prayitno Solly Aryza Sri Rejeki Sri Rejeki Sriwati Sriwati Steven Subandiyono Subandiyono Subekti, Mulat Suhendra Suminto Suminto Suminto Suminto Suminto Suminto Supono . Suradi Wijaya Saputra Suriyanti, Intan Susana E. Ratnawati Susanna Endah Ratnawati Sutrisno Anggoro Tita Elfitasari Titik Susilowati Tony Suhartono Tony Suhartono Tri W. Agustini Tri Winarni Agustini Tri Winarni Agustini Trienes, Yoni Trisnani Dwi Hapsari Tristiana Yunarti Vivi Endar VIVI ENDAR HERAWATI Vivi Endar Herawati Widianingsih Widianingsih Widodo S. Pranowo Widyawati Nurul Fajri Windarto, Seto YS Darmanto