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Journal : Journal of Innovation and Applied Technology

Supplementation Of α Tocopherol On Plasma Membrane Integrity Of Goat Sperm After Freezing Sri Wahjuningsih
Journal of Innovation and Applied Technology Vol 4, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.699 KB) | DOI: 10.21776/ub.jiat.004.01.7

Abstract

Problems that often arise in the process of freezing semen is  destruction of the plasma membrane because the sperm membrane contains unsaturated fatty acids are highly susceptible to peroxidation damage. α-tocopherol is an antioxidant that  reduce damage due to peroxidation. The purpose of the study was to determine α-tocopherol antioxidant supplementation in diluter on   plasma membrane integrity after freezing process. Semen was collected using artificial vagina from  goat aged  2 to 2.5 years in normal reproduction. The design used was randomized block design with treatment dose of a different α-tocopherol (0 grams, 0.2 grams, 0.4 grams, 0.6 grams) in 100 ml of extender. The results showed that the percentage  to   plasma membrane integrity equilibrated at a dose of 0.4 g (75.46%) was higher (P <0.05) compared with a dose of 0.0 g (73.78%), and the dose of 0.6 g (72.93%), but did not differ (P> 0.05 ) with a dose of 0.2 g (75.46%).  Plasma membrane integrity  examination results at  post thawing  at dose of 0.4 g (73.16%)was  higher (P <0.05) than a dose of 0.0 g (69.8%), 0.2 g (71.53%), 0.6 g (70.7 %) respectively. It was concluded that supplementation of antioxidant α-tocopherol 0.4 ml is the best dose to maintain  membrane integrity of semen frozen of goat.
Co-Authors Achadiah Rachmawati Aditya Budiawan Alfa Fajarrofa Angelina N. Tethool Anis Mei Munazaroh Anis Mei Munazaroh Anny Rosmayanti Anugra Yekti, Aulia Puspita Ardyah Ramadhina Irsanti Putri Aris Sutomo Asri Nurul Huda Aulani&#039;am Aulani&#039;am Aulia Puspita A Yekti Aulia Puspita Anugra Yekti Awang Tri Satria Ayu Melia Sades Bayuardhi, Muhammad Dedi Muhammad Dedy Kaunang Effendi, Fredyan Isnan Eko Widodo Eros Sukmawati Eros Sukmawati Ervandi, Mohamad Fahrudin Darlian Fajarrofa, Alfa Falah, Moh. Miftahul Gatot Cipatadi Gatot Ciptadi Hanief Eko Sulistyo Harsi, Tri Hartutik (Hartutik) Hartutik Hartutik Heraini, Dela Indiah Indiah Indiah, Indiah Indria Susanti Irfan Irfan Ita Wahju Nursita Khaeruddin Khaeruddin Kuswati Kuswati Ludi Ahmad Jalaludin M Nur Ihsan M. Nur Ihsan M. Pangestu Mashudi Mashudi Moch Sasmito Djati Moh Nur Ihsan Moh. Miftahul Falah Moh. Nur Ihsan Mohammad Nur Ihsan, Mohammad Nur Muhammad Agus Tahar Muhammad Nur Ihsan Muhammad Nur Ihsan Ndaru Pranowo Widiasmoro Nining Haryuni Nisa’us Sholikah Nurgiartiningsih, V. M. A. Nurlayasari, Irma Nurul Isnaini P. Srianto R. P. Putra Rahman Maulana Rahmawati, Achadiyah Rahmi, Siti Aulia REZA FAHLEVI Robbaani, Muhammad S. Suyadi Safitri, Brilla Ismaya Saka Wahyu Hikamawan Sepatria Jodiansyah Siti Sunami Sri Rahayu Suhartojo Hardjopranjoto Suhartojo Hardjopranjoto, Suhartojo Sutiman B. Sumitro Sutiman Bambang Sumitro Sutomo, Aris Suyadi Suyadi Tri Harsi Trinil Susilawati Trinil Susilawati Veronica Devita Bunga Widiasmoro, Ndaru Pranowo Wiyoso, Shelfia Anggraini Wulandari, Desinta Y A Tribudi Yanyan Setiawan Yulia Indri Anastasia