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All Journal Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner Journal of Innovation and Applied Technology Research Journal of Life Science Jurnal Ternak Tropika JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Tarjih Tropical Livestock Journal Jurnal Kedokteran Hewan Jurnal Agripet

Sri Wahjuningsih

Department Of Animal Production, Faculty Of Animal Husbandry, Brawijaya University

Author-ID : 2845722

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Cytoskeleton of Bovine Oocytes after Vitrification Using Ethylene Glycol Cryoprotectant Sri Wahjuningsih
Jurnal Kedokteran Hewan Vol 2, No 2 (2008): September
Publisher : Universitas Syiah Kuala

The objective of this research is to know the influence of vitrification method toward thecondition of bovine oocytes cytoskeleton based on microtubules using the immunohistochemistrytechnique. Oocytes classified in two treatments such as fresh oocytes (control) and oocytes withvitrification treatments of 10, 20, 30, 40, and 50% of ethylene glycol (EG). Data were analyzed bydescriptive analyzing. The results of this research indicated that vitrification treatments were affected tomicrotubules organization of oocytes. Concentration of EG 10, 20, 40, and 50% of EG could not repaircytoskeleton after vitrification, but cytoskeleton structure could be protected by EG 30%.

Reproductive Profile of Black Soldier Fly (Hermetia Illucens L.) on Different Rearing Substrates as Ruminant Feed Aulia Puspita Anugra Yekti; Nurul Isnaini; Sri Wahjuningsih; Ardyah Ramadhina Irsanti Putri; Hanief Eko Sulistyo
Jurnal Ilmu-Ilmu Peternakan Vol. 33 No. 2 (2023): August 2023
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2023.033.02.15

There is lack of evidence on the black soldier fly (Hermetia Illucens L.) and its use as a biological control and waste recycle agent. Unknown information related with mating and ovipositional activities. The aim of this research is to determine the reproductive profile and its manure of the Black Soldier Fly (Hermetia Illucens L.) through different rearing substrate as ruminant feed. A total of 4 treatments and 4 replicates were used in this experiment. The treatment diet was formulated as follows: T0: dry fermented cow waste, T1: 21% of crude protein, T2: 22% of protein, T3: 23% of protein. The data was analyzed using ODA SAS on an academic general linear model (GLM). The result showed using different rearing substrates gave insignificant differences (p > 0.05) on the reproductive profile, nutritional content, and manure nutritional content. In summary, the use of different leves of protein can be used as the rearing substrate without any adverse effects.

Effect of Thawing Technique on the Quality and Status of Acrosome Reactions Safitri, Brilla Ismaya; Wahjuningsih, Sri; Yekti, Aulia Puspita Anugra; Susilawati, Trinil
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 2 (2022): JITRO, May
Publisher : Universitas Halu Oleo

The success of Artificial Insemination is affected by the thawing technique used by the inseminator. The difference in thawing technique can affect the quality of the spermatozoa produced. This study aims to determine the effect of temperature and duration of thawing on the frozen semen quality of Bali and Madura cattle. The material used was 80 frozen semen straws for Bali cattle and Madura cattle. The method used is an experiment with four treatments and 10 replications, that is thawing in water at 37℃ for 30 seconds duration, 28℃ for 30 seconds, 28℃ for 45 seconds and 28℃ for 60 seconds. The variables observed in this study were capacitation, intact acrosome, intact plasma membrane and chromatin damage. The data were analyzed using a Randomized Block Design with replication as a block. The results of the analysis of variance showed that thawing technique had significant effect (p<0.05) on not yet capacitated, intact acrosome, intact plasma membrane. Thawing technique had no significant effect (p>0.05) on the acrosome reacted and chromatin damage.Keywords: thawing technique, capacitation, intact acrosome, intact plasma membrane, chromatin damage

The Effect of L-Arginine Supplementation on the Quality of Frozen Goat Semen Fahlevi, Reza; Wahjuningsih, Sri; Ihsan, Mohammad Nur
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 2 (2022): JITRO, May
Publisher : Universitas Halu Oleo

Various attempts have been made to maintain the quality of semen by using diluents that are added with appropriate antioxidants to protect spermatozoa from cold shock and maintain the quality of spermatozoa, while the addition of antioxidants in the diluent is useful for suppressing free radical reactions. One of the substances that can be added to cement thinner is L-Argine. The mechanism of action of L-Argine is the same as that of an antioxidant, which is useful in inhibiting free radicals. Nitric Oxide contained in L-Arginine is an antioxidant so that it can inactivate superoxide produced by spermatozoa during the oxygen consumption process. Shelters were carried out 2 times a week with the artificial vaginal method, with 10 shelters during the study. The semen that will be used for research has a minimum individual motility requirement of 70% and a minimum mass motility of ++. The variables observed in this study were abnormalities, intact plasma membrane and intact acrosome cap on observations before freezing and post thawing. The results of the analysis of variance showed that L-Arginine supplementation had no significant effect (p>0.05) on abnormalities in the pre-freezing and post-thawing observations, L-Arginine supplementation had a significant effect (p<0.05) on the integrity of the plasma membrane and acrosome cap. on observation before freezing and post thawing.Keywords: abnormalities, antioxidants, L-Argine, spermatozoa

Respon Superovulasi Sapi Persilangan Belgian Blue dengan Metode yang Berbeda Fahrudin Darlian; Sri Wahjuningsih; Anny Rosmayanti; Sepatria Jodiansyah; Ludi Ahmad Jalaludin; Yanyan Setiawan; Trinil Susilawati
Jurnal Agripet Vol 21, No 2 (2021): Volume 21, No. 2, Oktober 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i2.20407

ABSTRACT. Superovulasi merupakan suatu metode pemanfaatan induk betina unggul untuk menghasilkan embrio sebanyak-banyaknya untuk kegiatan transfer embrio. Tujuan penelitian ini membandingkan 2 teknik superovulasi yang berbeda pada sapi persilangan Belgian Blue. Penelitian dilakukan di Balai Embrio Ternak Cipelang Bogor dengan menggunakan 24 ekor sapi persilangan Belgian Blue dengan umur 2-3 tahun, masing-masing perlakuan 12 ekor. Penelitian dilakukan secara eksperimen dengan dua perlakuan, P1 = Superovulasi menggunakan penyuntikan FSH selama 3 hari pada pagi dan sore (metode konvensional) dengan dosis 400 mg FSH dalam 20 ml pelarut dan P2 = Superovulasi menggunakan penyuntikan tunggal (satu kali) FSH dengan dosis 400 mg FSH dalam 3 ml pelarut . Parameter pada penelitian ini adalah Jumlah Corpus luteum (CL), respon rate, perolehan embrio, kualitas embrio, recovery rate, dan tingkat fertilisasi. Data yang diperoleh diuji dengan uji T tidak berpasangan. Hasil analisa data menunjukkan hasil yang tidak berbeda nyata (P0,05) pada jumlah CL P1 : 8,42+3,06 dan P2 : 6,08+4,74; Respon rate P1 : 100% dan P2 : 75%; Total perolehan embrio P1 : 6,08+2,64 embrio dan P2 : 4,45+4,01 embrio; Embrio recovery rate P1 : 70,37+9,18% dan P2 : 61,33+12,12%; Embrio Layak Transfer P1 : 3,83+2,92 embrio dan P2 : 2,73+2,28 embrio; dan berbeda nyata (P0,05) pada rataan fertilisasi P1 : 79,10% dan P2 : 95,26%. Kesimpulan penelitian ini adalah teknik superovulasi penyuntikan tunggal FSH secara subkutan memberikan efek superovulasi dan menghasilkan embrio dengan jumlah dan kualitas yang tidak berbeda nyata dengan teknik superovulasi konvensional.(Superovulation Responses of Belgian Blue Crossbreed Cattle Treated with Different Superovulation Methods) ABSTRAK. Superovulation is a technique for producing a large number of embryos for embryo transfer using a genetically superior female. The purpose of this research was to compare two alternative methods of superovulation in Belgian Blue crossbreed cattle. The study used 24 Belgian Blue crossbred cattle aged 2-3 years, including 12 cows per treatment, at the National Livestock Embryo Center of Cipelang in Bogor. The research was done in an experimental setting using two different treatments, P1 = Superovulation using twice daily FSH injections for three days at a dose of 400 mg FSH dissolved in 20 ml of saline, and P2 = Superovulation using FSH single injections at a dose of 400 mg FSH dissolved in 3 ml of saline. The parameters in this study were the number of corpus luteum (CL), response rate, total number of embryo/ova collection, embryo quality, recovery rate, and fertilization rate. The data obtained were tested by unpaired T test. The results of data analysis showed results that were not significantly different (P0.05) on the number of CL P1 : 8.42+3.06 and P2 : 6.08+4.74; Response rate P1 : 100% and P2 : 75%; Total number of embryos collection P1 : 6.08+2.64 embryos and P2 : 4.45+4.01 embryos; Embryo recovery rate P1 : 70.37+9.18% and P2 : 61.33+12.12%; Transferable Embryos P1 : 3.83+2.92 embryos and P2 : 2.73+2.28 embryos; and significantly different (P0.05) on fertilization rate P1 : 79.10% and P2 : 95.26%. The conclusion of this study show that the subcutaneous FSH single injection technique induces superovulation and produces embryos that are similar in number and quality to those produced by conventional superovulation techniques.

Comparison of Embryo Production between Simmental and Limousin Cattle in Different Seasons at Cipelang Cattle Embryo Center Robbaani, Muhammad; Nurlayasari, Irma; Khaeruddin, Khaeruddin; Wahjuningsih, Sri
Tarjih Tropical Livestock Journal Vol. 4 No. 2 (2024): Tarjih-Tropical Livestock Journal
Publisher : Program Studi Peternakan Fakultas Sains dan Teknologi Universitas Muhammadiyah Sinjai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47030/trolija.v4i2.865

Indonesia is a tropical country that has two different seasons, namely the rainy season and the dry season. Embryo production can be influenced by several factors, one of which is the season factor. Seasonal factors can affect the quality and quantity of embryos produced. The purpose of this study was to determine the comparison of embryo production of Simmental cattle with Limousin cattle in the rainy season and the dry season. The material used was 40 donor cattle divided into 20 Simmental cattle and 20 Limousin cattle aged 3 to 6 years with a body weight of 450-700 kg. The research procedure starts from superovulation using reproductive hormone injection, artificial insemination, embryo collection using flushing technique and embryo evaluation. The research method used was a case study. Data processing and analysis in this study used the independent t-test with research variables of the number of embryos collected, the quality of embryos produced, the number of corpus luteum produced, the level of response, and the level of recovery. The results of this study were no significant difference (P> 0.05) in the production of Limousin and Simmental cattle embryos in the rainy season. In contrast to the dry season, the results were (P <0.05) which means there is a difference in the production of Limousin and Simmental cattle embryos in the dry season. On the other hand, Limousin cattle showed that Limousin cattle were slightly superior to all variables in the dry season.

Morphometric Characteristics and Body Indices as Selection Criteria for Female Bali Cattle in East Kalimantan Rahmi, Siti Aulia; Wahjuningsih, Sri; Nurgiartiningsih, Veronica Margareta Ani
Jurnal Ilmu-Ilmu Peternakan Vol. 35 No. 2 (2025): August 2025
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2025.035.02.7

Bali cattle constitute one of Indonesia's native germplasm sources for meat supply. Beef is one of the primary sources of protein that plays a crucial role in meeting the nutritional needs of the community. The research material consisted of 251 female Bali cattle aged 12 months and 24 months. These Bali cattle belong to the farmer group in Bontang city, Kutai Timur Regency (Kutim), Berau Regency, Kutai Kartanegara Regency (Kukar), Penajam Paser Utara Regency (PPU), and Paser Regency. The morphometric traits measured were withers height (WH), chest girth (CG), and body length (BL). The data obtained were analysed via analyses of variance, and if there were significant differences, the analysis was continued until a least significant difference was detected. The results revealed significant differences in the morphometric characteristics of 12-month-old Bali cattle across regions (P<0.01). The highest WH was from Bontang cattle (104.30±3.16a), and the lowest was from PPU (98.94±6.79c). The CG of Bontang cattle also had the highest value (131.30±6.29a), and the lowest value was from PPU (119.59±7.34b), with the highest BL of cattle from Berau (101.22±2.73a) and the lowest value from PPU (93.35±4.89b). Twenty-four-month-old female Bali cattle also presented significant morphometric differences (P<0.01) across regions. Cattle from the PPU consistently excelled in all parameters: WH (108.00±2.24a), CG (138.40±3.51), and BL (110.60±6.66a). The lowest WH was cattle from Berau at WH (101.33±0.58b), CG (126.00±2.65), and BL (97.33±4.62b). In conclusion, on the basis of observations of withers height, heart girth, and body length, all individual samples met the Indonesian National Standard (SNI) criteria for female Bali cattle breeding stock. Significant morphometric differences are caused by local genetic selection factors, the environment, feed availability, feed quality, and management practices.

Inseminasi Buatan Menggunakan Semen Beku Dosis Ganda Pada Sapi Perah Peranakan Friesian Holstein Yekti, Aulia Puspita Anugra; Bayuardhi, Muhammad; Wahjuningsih, Sri; Susilawati, Trinil
TERNAK TROPIKA Journal of Tropical Animal Production Vol. 24 No. 2 (2023): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2023.024.02.9

Inseminasi Buatan (IB) adalah suatu teknologi reproduksi yang dapat diimplementasikan untuk meningkatkan populasi dan mutu genetik ternak. Penelitian ini bertujuan untuk mengevaluasi keberhasilan IB dengan menggunakan dosis tunggal dan dosis ganda pada sapi peranakan Friesian Holstein (PFH). Penelitian ini dilakukan di desa Bendosari dan Sukamulya Kecamatan Pujon, Kabupaten Malang, Jawa Timur, Indonesia. Materi penelitian adalah sapi perah PFH sebanyak 99 ekor yang dipilih secara purposive sampling dengan kriteria yaitu memiliki BCS 2.5-3.5, sudah pernah melahirkan, mempunyai organ reproduksi yang sehat serta menunjukkan tanda estrus yang jelas. Perlakuan yang diberikan terdiri atas dua perlakuan yaitu 50 ekor sapi di IB dengan dosis tunggal (P0) dan 49 ekor sapi di IB dengan dosis ganda dengan waktu berbeda yaitu 2 dan 8 jam setelah munculnya estrus (P1). Deposisi semen yang digunakan adalah posisi 4 (korpus uteri). Pemeriksaan kebuntingan dilakukan dengan menggunakan ultrasonografi (USG). Parameter yang diamati adalah Non-Return Rate (NRR), Conception Rate (CR) dan Pregnancy Rate (PR). Analisa data dilakukan dengan menggunakan uji chi-square. Hasil penelitian menunjukkan bahwa persentase NRR1 dan NRR2 pada P0 adalah 70% dan 60%, sedangkan pada P1 adalah 81,63% dan 71,43%. Persentase CR pada P0 dan P1 masing-masing adalah 50% dan 57%, sedangkan nilai PR pada P0 dan P1 adalah 66% dan 67%. Hasil uji chi square menunjukkan nilai NRR dan CR menunjukkan hasil yang berbeda nyata (P<0,05), sedangkan PR tidak berbeda nyata (P>0,05). Kesimpulan pada penelitian ini bahwa metode IB semen beku dosis ganda pada waktu berbeda memiliki keberhasilan kebuntingan yang lebih baik dibandingkan dengan IB dosis tunggal.

SEASONAL VARIATIONS ON SEMEN CHARACTERISTICS OF FATTAILED AND GARUT RAMS UNDER TROPICAL CONDITIONS Isnaini, Nurul; Wahjuningsih, Sri; Falah, Moh. Miftahul; Fajarrofa, Alfa; Harsi, Tri; Sukmawati, Eros
Jurnal Kedokteran Hewan Vol 15, No 1 (2021): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i1.15168

This study investigated the influences of rainy and dry seasons on semen characteristics of fat-tailed and Garut rams under tropical conditions. A total of 4 healthy rams (2 fat-tailed rams and 2 Garut rams) aged 3 to 4 years old were used in this study. The semen was collected from each ram during November 2016 to February 2017 (rainy season) and May to August 2017 (dry season). The measurement of semen characteristics including semen volume (SV), sperm concentration (SpC), total sperm (TSp), individual sperm motility (ISM), post-thawing sperm motility (PTSM), and frozen semen production (FSP). The rainy season resulted higher SV (P= 0.021), TSp (P= 0.005), ISM (P= 0.028), and FSP (P= 0.005) than the dry season. On the other hand, fat-tailed rams had higher (P0.001) SV, TSp, and FSP compared to Garut rams. There was no interaction (P0.05) between season and breed on all semen characteristic parameters found in the present study. It can be concluded that the semen collection during the rainy season had a beneficial impact to produce high-quality semen for artificial insemination program than in the dry season. Fat-tailed rams have better semen characteristics compared to Garut rams.

The Effect of Centrifugation on Semen Quality of Peranakan Ettawah Goat's Post Thawing Indiah, Indiah; Wahjuningsih, Sri
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i2.9809

The aim of the research was to determine the optimum of centrifugation on the quality of Peranakan Ettawah goat's spermatozoa prepared for in vitro fertilization preparation.The research of material was frozen semen ofPeranakan Ettawah goat produced by Artificial Insemination Center in Singosari with minimum post thawing motility in 40%. The method of the research was an experiment method with the four treatment, i.e: 1000 (P1), 1500 (P2), 2000 rpm (P3) of different centrifugation. The variable observes were spermatozoa motility, spermatozoa viability, and abnormal morphology of spermatozoa. Data obtain was analyzed statistically using Completely Randomize Design and continued with Tukey test. The result showed that on the upper layer of each treatment (P1, P2, and P3) obtained 55,723,55; 69,553,35; and 55,192,72% for spermatozoa motility, 62,995,87; 73,994,36; and 57,399,22% for spermatozoa viability, and 7,193,64; 8,842,65; and 6,403,00% for abnormal morphology of spermatozoa, descriptively. While on the lower layer the result showed 55,593,99; 68,633,88; and57,612,20% for spermatozoa motility, 70,057,47; 77,441,08; and 69,9311,98% for spermatozoa viability, and 10,366,20; 9,553,27; and 8,092,80% for abnormal morphology of spermatozoa, desciptively. It was concluded that centrifugation in 1500 rpm showed the highest motility and viability on the upper layer and lower layer.

Co-Authors Achadiah Rachmawati Aditya Budiawan Alfa Fajarrofa Angelina N. Tethool Anis Mei Munazaroh Anis Mei Munazaroh Anny Rosmayanti Anugra Yekti, Aulia Puspita Ardyah Ramadhina Irsanti Putri Aris Sutomo Asri Nurul Huda Aulani'am Aulani'am Aulia Puspita A Yekti Aulia Puspita Anugra Yekti Awang Tri Satria Ayu Melia Sades Bayuardhi, Muhammad Dedi Muhammad Dedy Kaunang Effendi, Fredyan Isnan Eko Widodo Eros Sukmawati Eros Sukmawati Ervandi, Mohamad Fahrudin Darlian Fajarrofa, Alfa Falah, Moh. Miftahul Gatot Cipatadi Gatot Ciptadi Hanief Eko Sulistyo Harsi, Tri Hartutik (Hartutik) Hartutik Hartutik Indiah Indiah Indiah, Indiah Indria Susanti Irfan Irfan Khaeruddin Khaeruddin Kuswati Kuswati Ludi Ahmad Jalaludin M Nur Ihsan M. Nur Ihsan M. Pangestu Mashudi Mashudi Moch Sasmito Djati Moh Nur Ihsan Moh. Miftahul Falah Moh. Nur Ihsan Mohammad Nur Ihsan, Mohammad Nur Muhammad Agus Tahar Muhammad Nur Ihsan Muhammad Nur Ihsan Ndaru Pranowo Widiasmoro Nining Haryuni Nisa’us Sholikah Nurgiartiningsih, V. M. A. Nurlayasari, Irma Nurul Isnaini P. Srianto R. P. Putra Rahman Maulana Rahmi, Siti Aulia REZA FAHLEVI Robbaani, Muhammad S. Suyadi Safitri, Brilla Ismaya Saka Wahyu Hikamawan Sepatria Jodiansyah Siti Sunami Sri Rahayu Suhartojo Hardjopranjoto Suhartojo Hardjopranjoto, Suhartojo Sutiman B. Sumitro Sutiman Bambang Sumitro Sutomo, Aris Suyadi Suyadi Tri Harsi Trinil Susilawati Trinil Susilawati Veronica Devita Bunga Widiasmoro, Ndaru Pranowo Wulandari, Desinta Y A Tribudi Yanyan Setiawan Yulia Indri Anastasia

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