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All Journal Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner Journal of Innovation and Applied Technology Research Journal of Life Science Jurnal Ternak Tropika JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Tarjih Tropical Livestock Journal Jurnal Kedokteran Hewan Jurnal Agripet
Sri Wahjuningsih
Department Of Animal Production, Faculty Of Animal Husbandry, Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Cytoskeleton of Bovine Oocytes after Vitrification Using Ethylene Glycol Cryoprotectant Sri Wahjuningsih
Jurnal Kedokteran Hewan Vol 2, No 2 (2008): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5023.182 KB) | DOI: 10.21157/j.ked.hewan.v2i2.9780

Abstract

The objective of this research is to know the influence of vitrification method toward thecondition of bovine oocytes cytoskeleton based on microtubules using the immunohistochemistrytechnique. Oocytes classified in two treatments such as fresh oocytes (control) and oocytes withvitrification treatments of 10, 20, 30, 40, and 50% of ethylene glycol (EG). Data were analyzed bydescriptive analyzing. The results of this research indicated that vitrification treatments were affected tomicrotubules organization of oocytes. Concentration of EG 10, 20, 40, and 50% of EG could not repaircytoskeleton after vitrification, but cytoskeleton structure could be protected by EG 30%.
Cytoskeleton of Bovine Oocytes after Vitrification Using Ethylene Glycol Cryoprotectant Sri Wahjuningsih
Jurnal Kedokteran Hewan Vol 2, No 2 (2008): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v2i2.9780

Abstract

The objective of this research is to know the influence of vitrification method toward thecondition of bovine oocytes cytoskeleton based on microtubules using the immunohistochemistrytechnique. Oocytes classified in two treatments such as fresh oocytes (control) and oocytes withvitrification treatments of 10, 20, 30, 40, and 50% of ethylene glycol (EG). Data were analyzed bydescriptive analyzing. The results of this research indicated that vitrification treatments were affected tomicrotubules organization of oocytes. Concentration of EG 10, 20, 40, and 50% of EG could not repaircytoskeleton after vitrification, but cytoskeleton structure could be protected by EG 30%.
ULTRASTRUKTUR OOSIT KAMBING PASCA KRIOPRESERVASI DENGAN METODE VITRIFIKASI Sri Wahjuningsih; Sasmito Djati
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i2.915

Abstract

Tujuan penelitian adalah mengetahui ultrastruktur oosit pasca vitrifikasi menggunakan transmisi elektron mikroskop. Ovarium dikumpulkan dari rumah potong hewan dan oosit dikoleksi dengan cara aspirasi dari folikel menggunakan jarum ukuran 18 G. Setelah dilakukan klasifikasi kualitas  oosit,  maka  oosit  yang  berkualitas  A  dimaturasi  secara  in  vitro. Oosit   hasil  maturasi  dipaparkan  ke  dalam  larutan  vitrifikasi  yang mengandung 30%  EG  + 0,5 M  sukrosa  dengan  lama  waktu paparan  3 menit,  oosit  dimasukkan  ke dalam  ministraw  0,25 cc  (French  straw), kemudian dipaparkan pada uap nitrogen selama 10 detik dan dimasukkan dalam kontainer nitrogen cair. Setelah 3 minggu ministraw diambil dan dilakukan penghangatan (warming) di udara selama 10 detik, dimasukkan dalam waterbath suhu 35 C selama 30 detik. Isi ministraw dituangkan ke dalam cawan petri dan oosit dibilas dua kali dengan  sukrosa 0,5 M.  Selanjutnya dilakukan pengamatan ultrastruktur oosit dengan transmisi elektron mikroskop. Hasil penelitian menunjukkan oosit hasil vitrifikasi mempunyai zona pelusida yang abnormal (fraktur), mem bran plasma mengalami lisis, beberapa butir korteks mengalami degenerasi dan perpindahan butir korteks di ruang perivitelin. Kesimpulan dari penelitian ini adalah terdapat perubahan ultrastruktur pada oosit setelah vitrifikasi.
KONSENTRASI GONADOTROPIN RELEASING HORMONE (GnRH) EKSTRAK OTAK SAPI PERANAKAN FRIESIEN HOLSTEIN BETINA FASE FOLIKULER DAN LUTEAL Nurul Isnaini; Sri Wahjuningsih
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i2.2627

Abstract

Penelitian ini bertujuan mengidentifikasi kandungan gonadotropin releasing hormone (GnRH) ekstrak jaringan otak sapi dewasa pada fase folikuler (ovari berfolikel) dan fase luteal (ovari berkorpus luteum). Pada penelitian ini digunakan 2 buah otak sapi perah peranakan Friesien Holstein (FH) dewasa dengan umur 7-8 tahun yang pada saat disembelih kondisi ovarinya menunjukkan fase folikuler (1 buah otak) dan fase luteal (1 buah otak). Analisis kandungan GnRH dilakukan dengan metode enzyme-linked immunosorbent assay (ELISA) indirect. Hasil penelitian menunjukkan bahwa konsentrasi GnRH bagian otak sapi pada fase folikuler vs fase luteal berturut-turut untuk bagian otak parietal lobe of cerebral hemisphere, corpus callosum, thalamus, hipothalamus, pituitary gland (hipofise), midbrain, cerebellum, pons, dan medulla oblongata masing-masing adalah 260 vs 270, 110 vs 170, 230 vs 320, 1590 vs 1310, 840 vs 1250, 530 vs 810, 200 vs 480, 230 vs 100, dan 220 vs 70 μg/ml. Dari hasil penelitian disimpulkan bahwa semua bagian otak sapi yang diamati mengandung GnRH dengan konsentrasi yang berbeda antara fase folikuler dengan fase luteal.
Effect of Ethylene Glycol Concentration and Length of Exposure on In Vitro Fertility of Bovine Oocyte Sri Wahjuningsih; Suhartojo Hardjopranjoto; Sutiman Bambang Sumitro
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i2.3097

Abstract

The purpose of the study was to determine the influence of the concentration of ethylene glycol (EG) and length of exposure to levels of bovine oocytes in vitro fertility. This research was conducted using a completely randomized design factorial 5x3 with 7 replicates. The first factor was the concentration of cryoprotectants EG 10, 20, 30, 40, and 50%. The second factor was the length of exposure 1, 3, and 5 minutes. The results showed that the concentration of EG and the length of exposure were effect on fertilized oocytes (P0.05). The level of oocytes in vitro fertility after vitrification in 30% EG and long exposure to 3 minutes did not different (P0.05) compared to fresh oocytes, while the EG treatment 10, 20, 40, and 50% significantly showed a lower fertility rate than the 30% EG (P0.05). It was concluded that the concentration of EG and length of exposure were effect on fertilized oocytes. The highest oocytes in vitro fertility level were found in 30% EG concentration with 3 minutes exposure time.
SEASONAL VARIATIONS ON SEMEN CHARACTERISTICS OF FATTAILED AND GARUT RAMS UNDER TROPICAL CONDITIONS Nurul Isnaini; Sri Wahjuningsih; Moh. Miftahul Falah; Alfa Fajarrofa; Tri Harsi; Eros Sukmawati
Jurnal Kedokteran Hewan Vol 15, No 1 (2021): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i1.15168

Abstract

This study investigated the influences of rainy and dry seasons on semen characteristics of fat-tailed and Garut rams under tropical conditions. A total of 4 healthy rams (2 fat-tailed rams and 2 Garut rams) aged 3 to 4 years old were used in this study. The semen was collected from each ram during November 2016 to February 2017 (rainy season) and May to August 2017 (dry season). The measurement of semen characteristics including semen volume (SV), sperm concentration (SpC), total sperm (TSp), individual sperm motility (ISM), post-thawing sperm motility (PTSM), and frozen semen production (FSP). The rainy season resulted higher SV (P= 0.021), TSp (P= 0.005), ISM (P= 0.028), and FSP (P= 0.005) than the dry season. On the other hand, fat-tailed rams had higher (P0.001) SV, TSp, and FSP compared to Garut rams. There was no interaction (P0.05) between season and breed on all semen characteristic parameters found in the present study. It can be concluded that the semen collection during the rainy season had a beneficial impact to produce high-quality semen for artificial insemination program than in the dry season. Fat-tailed rams have better semen characteristics compared to Garut rams.
The Effect of Centrifugation on Semen Quality of Peranakan Ettawah Goat's Post Thawing Indiah Indiah; Sri Wahjuningsih
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i2.9809

Abstract

The aim of the research was to determine the optimum of centrifugation on the quality of Peranakan Ettawah goat's spermatozoa prepared for in vitro fertilization preparation.The research of material was frozen semen ofPeranakan Ettawah goat produced by Artificial Insemination Center in Singosari with minimum post thawing motility in 40%. The method of the research was an experiment method with the four treatment, i.e: 1000 (P1), 1500 (P2), 2000 rpm (P3) of different centrifugation. The variable observes were spermatozoa motility, spermatozoa viability, and abnormal morphology of spermatozoa. Data obtain was analyzed statistically using Completely Randomize Design and continued with Tukey test. The result showed that on the upper layer of each treatment (P1, P2, and P3) obtained 55,72±3,55; 69,55±3,35; and 55,19±2,72% for spermatozoa motility, 62,99±5,87; 73,99±4,36; and 57,39±9,22% for spermatozoa viability, and 7,19±3,64; 8,84±2,65; and 6,40±3,00% for abnormal morphology of spermatozoa, descriptively. While on the lower layer the result showed 55,59±3,99; 68,63±3,88; and57,61±2,20% for spermatozoa motility, 70,05±7,47; 77,44±1,08; and 69,93±11,98% for spermatozoa viability, and 10,36±6,20; 9,55±3,27; and 8,09±2,80% for abnormal morphology of spermatozoa, desciptively. It was concluded that centrifugation in 1500 rpm showed the highest motility and viability on the upper layer and lower layer.
Reproductive Profile of Black Soldier Fly (Hermetia Illucens L.) on Different Rearing Substrates as Ruminant Feed Aulia Puspita Anugra Yekti; Nurul Isnaini; Sri Wahjuningsih; Ardyah Ramadhina Irsanti Putri; Hanief Eko Sulistyo
Jurnal Ilmu-Ilmu Peternakan Vol. 33 No. 2 (2023): August 2023
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2023.033.02.15

Abstract

There is lack of evidence on the black soldier fly (Hermetia Illucens L.) and its use as a biological control and waste recycle agent. Unknown information related with mating and ovipositional activities. The aim of this research is to determine the reproductive profile and its manure of the Black Soldier Fly (Hermetia Illucens L.) through different rearing substrate as ruminant feed. A total of 4 treatments and 4 replicates were used in this experiment. The treatment diet was formulated as follows:    T0:  dry fermented cow waste, T1: 21% of crude protein, T2: 22% of protein, T3: 23% of protein. The data was analyzed using ODA SAS on an academic general linear model (GLM).  The result showed using different rearing substrates gave insignificant differences (p > 0.05) on the reproductive profile, nutritional content, and manure nutritional content. In summary, the use of different leves of protein can be used as the rearing substrate without any adverse effects.
Inseminasi Buatan Menggunakan Semen Beku Dosis Ganda Pada Sapi Perah Peranakan Friesian Holstein Aulia Puspita Anugra Yekti; Muhammad Bayuardhi; Sri Wahjuningsih; Trinil Susilawati
TERNAK TROPIKA Journal of Tropical Animal Production Vol. 24 No. 2 (2023): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtapro.2023.024.02.9

Abstract

Inseminasi Buatan (IB) adalah suatu teknologi reproduksi yang dapat diimplementasikan untuk meningkatkan populasi dan mutu genetik ternak. Penelitian ini bertujuan untuk mengevaluasi keberhasilan IB dengan menggunakan dosis tunggal dan dosis ganda pada sapi peranakan Friesian Holstein (PFH). Penelitian ini dilakukan di desa Bendosari dan Sukamulya Kecamatan Pujon, Kabupaten Malang, Jawa Timur, Indonesia. Materi penelitian adalah sapi perah PFH sebanyak 99 ekor yang dipilih secara purposive sampling dengan kriteria yaitu memiliki BCS 2.5-3.5, sudah pernah melahirkan, mempunyai organ reproduksi yang sehat serta menunjukkan tanda estrus yang jelas. Perlakuan yang diberikan terdiri atas dua perlakuan yaitu 50 ekor sapi di IB dengan dosis tunggal (P0) dan 49 ekor sapi di IB dengan dosis ganda dengan waktu berbeda yaitu 2 dan 8 jam setelah munculnya estrus (P1). Deposisi semen yang digunakan adalah posisi 4 (korpus uteri). Pemeriksaan kebuntingan dilakukan dengan menggunakan ultrasonografi (USG). Parameter yang diamati adalah Non-Return Rate (NRR), Conception Rate (CR) dan Pregnancy Rate (PR). Analisa data dilakukan dengan menggunakan uji chi-square. Hasil penelitian menunjukkan bahwa persentase NRR1 dan NRR2 pada P0 adalah 70% dan 60%, sedangkan pada P1 adalah 81,63% dan 71,43%. Persentase CR pada P0 dan P1 masing-masing adalah 50% dan 57%, sedangkan nilai PR pada P0 dan P1 adalah 66% dan 67%. Hasil uji chi square menunjukkan nilai NRR dan CR menunjukkan hasil yang berbeda nyata (P<0,05), sedangkan PR tidak berbeda nyata (P>0,05). Kesimpulan pada penelitian ini bahwa metode IB semen beku dosis ganda pada waktu berbeda memiliki keberhasilan kebuntingan yang lebih baik dibandingkan dengan IB dosis tunggal.
Effect of Thawing Technique on the Quality and Status of Acrosome Reactions Safitri, Brilla Ismaya; Wahjuningsih, Sri; Yekti, Aulia Puspita Anugra; Susilawati, Trinil
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 2 (2022): JITRO, May
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (593.633 KB) | DOI: 10.33772/jitro.v9i2.23126

Abstract

The success of Artificial Insemination is affected by the thawing technique used by the inseminator. The difference in thawing technique can affect the quality of the spermatozoa produced. This study aims to determine the effect of temperature and duration of thawing on the frozen semen quality of Bali and Madura cattle. The material used was 80 frozen semen straws for Bali cattle and Madura cattle. The method used is an experiment with four treatments and 10 replications, that is thawing in water at 37℃ for 30 seconds duration, 28℃ for 30 seconds, 28℃ for 45 seconds and 28℃ for 60 seconds. The variables observed in this study were capacitation, intact acrosome, intact plasma membrane and chromatin damage.  The data were analyzed using a Randomized Block Design with replication as a block. The results of the analysis of variance showed that thawing technique had significant effect (p<0.05) on not yet capacitated, intact acrosome, intact plasma membrane.  Thawing technique had no significant effect (p>0.05) on the acrosome reacted and chromatin damage.Keywords:  thawing technique, capacitation, intact acrosome, intact plasma membrane, chromatin damage
Co-Authors Achadiah Rachmawati Aditya Budiawan Alfa Fajarrofa Angelina N. Tethool Anis Mei Munazaroh Anis Mei Munazaroh Anny Rosmayanti Anugra Yekti, Aulia Puspita Ardyah Ramadhina Irsanti Putri Aris Sutomo Asri Nurul Huda Aulani&#039;am Aulani&#039;am Aulia Puspita A Yekti Aulia Puspita Anugra Yekti Awang Tri Satria Ayu Melia Sades Dedi Muhammad Dedy Kaunang Effendi, Fredyan Isnan Eko Widodo Eros Sukmawati Ervandi, Mohamad Fahrudin Darlian Gatot Cipatadi Gatot Ciptadi Hanief Eko Sulistyo Hartutik (Hartutik) Hartutik Hartutik Indiah Indiah Indria Susanti Irfan Irfan Khaeruddin Khaeruddin Kuswati Kuswati Ludi Ahmad Jalaludin M Nur Ihsan M. Nur Ihsan M. Pangestu Mashudi Mashudi Moch Sasmito Djati Moh Nur Ihsan Moh. Miftahul Falah Moh. Nur Ihsan Mohammad Nur Ihsan, Mohammad Nur Muhammad Agus Tahar Muhammad Bayuardhi Muhammad Nur Ihsan Muhammad Nur Ihsan Ndaru Pranowo Widiasmoro Nining Haryuni Nisa’us Sholikah Nurgiartiningsih, V. M. A. Nurlayasari, Irma Nurul Isnaini P. Srianto R. P. Putra Rahman Maulana Rahmi, Siti Aulia REZA FAHLEVI Robbaani, Muhammad S. Suyadi Safitri, Brilla Ismaya Saka Wahyu Hikamawan Sepatria Jodiansyah Siti Sunami Sri Rahayu Suhartojo Hardjopranjoto Sutiman Bambang Sumitro Suyadi Suyadi Tri Harsi Trinil Susilawati Trinil Susilawati Trinil Susilawati Veronica Devita Bunga Wulandari, Desinta Y A Tribudi Yanyan Setiawan Yulia Indri Anastasia