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All Journal Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu Ternak Veteriner Journal of Innovation and Applied Technology Research Journal of Life Science Jurnal Ternak Tropika JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Tarjih Tropical Livestock Journal Jurnal Kedokteran Hewan Jurnal Agripet
Sri Wahjuningsih
Department Of Animal Production, Faculty Of Animal Husbandry, Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology Veterinary Other

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The Effect of L-Arginine Supplementation on the Quality of Frozen Goat Semen Fahlevi, Reza; Wahjuningsih, Sri; Ihsan, Mohammad Nur
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 2 (2022): JITRO, May
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (424.285 KB) | DOI: 10.33772/jitro.v9i2.22616

Abstract

Various attempts have been made to maintain the quality of semen by using diluents that are added with appropriate antioxidants to protect spermatozoa from cold shock and maintain the quality of spermatozoa, while the addition of antioxidants in the diluent is useful for suppressing free radical reactions. One of the substances that can be added to cement thinner is L-Argine. The mechanism of action of L-Argine is the same as that of an antioxidant, which is useful in inhibiting free radicals. Nitric Oxide contained in L-Arginine is an antioxidant so that it can inactivate superoxide produced by spermatozoa during the oxygen consumption process. Shelters were carried out 2 times a week with the artificial vaginal method, with 10 shelters during the study. The semen that will be used for research has a minimum individual motility requirement of 70% and a minimum mass motility of ++. The variables observed in this study were abnormalities, intact plasma membrane and intact acrosome cap on observations before freezing and post thawing. The results of the analysis of variance showed that L-Arginine supplementation had no significant effect (p>0.05) on abnormalities in the pre-freezing and post-thawing observations, L-Arginine supplementation had a significant effect (p<0.05) on the integrity of the plasma membrane and acrosome cap. on observation before freezing and post thawing.Keywords:  abnormalities, antioxidants, L-Argine, spermatozoa
Respon Superovulasi Sapi Persilangan Belgian Blue dengan Metode yang Berbeda Fahrudin Darlian; Sri Wahjuningsih; Anny Rosmayanti; Sepatria Jodiansyah; Ludi Ahmad Jalaludin; Yanyan Setiawan; Trinil Susilawati
Jurnal Agripet Vol 21, No 2 (2021): Volume 21, No. 2, Oktober 2021
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v21i2.20407

Abstract

ABSTRACT. Superovulasi merupakan suatu metode pemanfaatan induk betina unggul untuk menghasilkan embrio sebanyak-banyaknya untuk kegiatan transfer embrio. Tujuan penelitian ini membandingkan 2 teknik superovulasi yang berbeda pada sapi persilangan Belgian Blue. Penelitian dilakukan di Balai Embrio Ternak Cipelang Bogor dengan menggunakan 24 ekor sapi persilangan Belgian Blue dengan umur 2-3 tahun, masing-masing perlakuan 12 ekor. Penelitian dilakukan secara eksperimen dengan dua perlakuan, P1 = Superovulasi menggunakan penyuntikan FSH selama 3 hari pada pagi dan sore (metode konvensional) dengan dosis 400 mg FSH dalam 20 ml pelarut dan P2 = Superovulasi menggunakan penyuntikan tunggal (satu kali) FSH dengan dosis 400 mg FSH dalam 3 ml pelarut . Parameter pada penelitian ini adalah Jumlah Corpus luteum (CL), respon rate, perolehan embrio, kualitas embrio, recovery rate, dan tingkat fertilisasi. Data yang diperoleh diuji dengan uji T tidak berpasangan. Hasil analisa data menunjukkan hasil yang tidak berbeda nyata (P0,05) pada jumlah CL P1 : 8,42+3,06 dan P2 : 6,08+4,74; Respon rate P1 : 100% dan P2 : 75%; Total perolehan embrio P1 : 6,08+2,64 embrio dan P2 : 4,45+4,01 embrio; Embrio recovery rate P1 : 70,37+9,18% dan P2 : 61,33+12,12%; Embrio Layak Transfer P1 : 3,83+2,92 embrio dan P2 : 2,73+2,28 embrio; dan berbeda nyata (P0,05) pada rataan fertilisasi P1 : 79,10% dan P2 : 95,26%. Kesimpulan penelitian ini adalah teknik superovulasi penyuntikan tunggal FSH secara subkutan memberikan efek superovulasi dan menghasilkan embrio dengan jumlah dan kualitas yang tidak berbeda nyata dengan teknik superovulasi konvensional.(Superovulation Responses of Belgian Blue Crossbreed Cattle Treated with Different Superovulation Methods) ABSTRAK. Superovulation is a technique for producing a large number of embryos for embryo transfer using a genetically superior female. The purpose of this research was to compare two alternative methods of superovulation in Belgian Blue crossbreed cattle. The study used 24 Belgian Blue crossbred cattle aged 2-3 years, including 12 cows per treatment, at the National Livestock Embryo Center of Cipelang in Bogor. The research was done in an experimental setting using two different treatments, P1 = Superovulation using twice daily FSH injections for three days at a dose of 400 mg FSH dissolved in 20 ml of saline, and P2 = Superovulation using FSH single injections at a dose of 400 mg FSH dissolved in 3 ml of saline. The parameters in this study were the number of corpus luteum (CL), response rate, total number of embryo/ova collection, embryo quality, recovery rate, and fertilization rate. The data obtained were tested by unpaired T test. The results of data analysis showed results that were not significantly different (P0.05) on the number of CL P1 : 8.42+3.06 and P2 : 6.08+4.74; Response rate P1 : 100% and P2 : 75%; Total number of embryos collection P1 : 6.08+2.64 embryos and P2 : 4.45+4.01 embryos; Embryo recovery rate P1 : 70.37+9.18% and P2 : 61.33+12.12%; Transferable Embryos P1 : 3.83+2.92 embryos and P2 : 2.73+2.28 embryos; and significantly different (P0.05) on fertilization rate P1 : 79.10% and P2 : 95.26%. The conclusion of this study show that the subcutaneous FSH single injection technique induces superovulation and produces embryos that are similar in number and quality to those produced by conventional superovulation techniques.
Hubungan antara Tingkah Laku Seksual dengan Produksi Spermatozoa Sapi Brahman Nisa&#039;us Sholikah; Aris Sutomo; Ndaru Pranowo Widiasmoro; Sri Wahjuningsih; Aulia Puspita Anugra Yekti; Kuswati Kuswati; Trinil Susilawati
Jurnal Agripet Vol 18, No 2 (2018): Volume 18, No. 2, Oktober 2018
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v18i2.12036

Abstract

ABSTRAK. Pejantan untuk program inseminasi buatan adalah pejantan unggul yang diseleksi berdasarkan libido dan produksi spermatozoa. Penelitian bertujuan untuk menganalisa hubungan antara tingkah laku seksual dengan produksi spermatozoa sapi Brahman. Materi penelitian adalah 3 pejantan sapi Brahman yang ditampung menggunakan vagina buatan. Metode penelitian adalah observasional dengan mengamati waktu reaksi dan lama ejakulasi. Observasi laboratorium dengan mengamati volume, konsentrasi, dan motilitas individu untuk menghitung total spermatozoa (TS) dan total spermatozoa motil (TSM). Pengamatan dilakukan sebanyak 10 ulangan. Data dianalisa secara deskriptif serta hubungan antara tingkah laku seksual dan produksi spermatozoa dihitung dengan korelasi Pearson. Hasil penelitian menunjukkan terdapat variasi tingkah laku seksual dan produksi spermatozoa pada sapi Brahman. Individu Cipta mempunyai waktu reaksi dan lama ejakulasi paling singkat serta produksi spermatozoa paling rendah. Hubungan signifikan antara waktu reaksi dengan TS serta TSM pada individu Bizzyard dengan koefisien determinasi (R2) sebesar 14 % dan 20%. Hubungan sangat signifikan antara lama ejakulasi dengan TS serta TSM pada individu Cipta dengan R2 sebesar 46% dan 58%. Kesimpulan penelitian adalah terdapat hubungan antara tingkah laku seksual dengan produksi spermatozoa pada sapi Brahman, dengan koefisien korelasi (r) tertinggi sebesar 0,72 dan R2 sebesar 58% antara lama ejakulasi dan total spermatozoa motil pada individu Cipta.(The relationship between sexual behavior and semen production in Brahman bulls)ABSTRACT. Bulls for artificial insemination must be selected based on libido and sperm production. The study aimed to analyze the relationship between sexual behavior with sperm production on Brahman bulls. The material was 3 Brahman bulls collected using artificial vagina. The method was observational by observing reaction time and ejaculation duration. Laboratory observations by observing volume, concentration, and motility to calculate total sperm (TS) and total motile sperm counts (TMSC). Observations were replicated 10 times. The data were analyzed descriptively, the relationship between behavior and sperm production was examined using Pearson correlation. The results showed that there were variations in sexual behavior and sperm production in each bull. Cipta have the shortest reaction time and ejaculation duration and the lowest sperm production. A significant relationship between reaction time and TS and TMSC in Bizzyard with coefficient of determination (R2) of 14% and 20%. A very significant between ejaculation duration with TS and TMSC in Cipta with R2 of 46% and 58%. In conclusion, there was a relationship between sexual behavior and sperm production in Brahman bulls, with the highest correlation coefficient (r) of 0.72 and R2 of 58% between ejaculation duration and the total motile sperm counts in Cipta.
Comparison of Embryo Production between Simmental and Limousin Cattle in Different Seasons at Cipelang Cattle Embryo Center Robbaani, Muhammad; Nurlayasari, Irma; Khaeruddin, Khaeruddin; Wahjuningsih, Sri
Tarjih Tropical Livestock Journal Vol. 4 No. 2 (2024): Tarjih-Tropical Livestock Journal
Publisher : Program Studi Peternakan Fakultas Sains dan Teknologi Universitas Muhammadiyah Sinjai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47030/trolija.v4i2.865

Abstract

Indonesia is a tropical country that has two different seasons, namely the rainy season and the dry season. Embryo production can be influenced by several factors, one of which is the season factor. Seasonal factors can affect the quality and quantity of embryos produced. The purpose of this study was to determine the comparison of embryo production of Simmental cattle with Limousin cattle in the rainy season and the dry season. The material used was 40 donor cattle divided into 20 Simmental cattle and 20 Limousin cattle aged 3 to 6 years with a body weight of 450-700 kg. The research procedure starts from superovulation using reproductive hormone injection, artificial insemination, embryo collection using flushing technique and embryo evaluation. The research method used was a case study. Data processing and analysis in this study used the independent t-test with research variables of the number of embryos collected, the quality of embryos produced, the number of corpus luteum produced, the level of response, and the level of recovery. The results of this study were no significant difference (P> 0.05) in the production of Limousin and Simmental cattle embryos in the rainy season. In contrast to the dry season, the results were (P <0.05) which means there is a difference in the production of Limousin and Simmental cattle embryos in the dry season. On the other hand, Limousin cattle showed that Limousin cattle were slightly superior to all variables in the dry season.
Morphometric Characteristics and Body Indices as Selection Criteria for Female Bali Cattle in East Kalimantan Rahmi, Siti Aulia; Wahjuningsih, Sri; Nurgiartiningsih, Veronica Margareta Ani
Jurnal Ilmu-Ilmu Peternakan Vol. 35 No. 2 (2025): August 2025
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2025.035.02.7

Abstract

Bali cattle constitute one of Indonesia's native germplasm sources for meat supply. Beef is one of the primary sources of protein that plays a crucial role in meeting the nutritional needs of the community. The research material consisted of 251 female Bali cattle aged 12 months and 24 months. These Bali cattle belong to the farmer group in Bontang city, Kutai Timur Regency (Kutim), Berau Regency, Kutai Kartanegara Regency (Kukar), Penajam Paser Utara Regency (PPU), and Paser Regency. The morphometric traits measured were withers height (WH), chest girth (CG), and body length (BL). The data obtained were analysed via analyses of variance, and if there were significant differences, the analysis was continued until a least significant difference was detected. The results revealed significant differences in the morphometric characteristics of 12-month-old Bali cattle across regions (P<0.01). The highest WH was from Bontang cattle (104.30±3.16a), and the lowest was from PPU (98.94±6.79c). The CG of Bontang cattle also had the highest value (131.30±6.29a), and the lowest value was from PPU (119.59±7.34b), with the highest BL of cattle from Berau (101.22±2.73a) and the lowest value from PPU (93.35±4.89b). Twenty-four-month-old female Bali cattle also presented significant morphometric differences (P<0.01) across regions. Cattle from the PPU consistently excelled in all parameters: WH (108.00±2.24a), CG (138.40±3.51), and BL (110.60±6.66a). The lowest WH was cattle from Berau at WH (101.33±0.58b), CG (126.00±2.65), and BL (97.33±4.62b). In conclusion, on the basis of observations of withers height, heart girth, and body length, all individual samples met the Indonesian National Standard (SNI) criteria for female Bali cattle breeding stock. Significant morphometric differences are caused by local genetic selection factors, the environment, feed availability, feed quality, and management practices.
Co-Authors Achadiah Rachmawati Aditya Budiawan Alfa Fajarrofa Angelina N. Tethool Anis Mei Munazaroh Anis Mei Munazaroh Anny Rosmayanti Anugra Yekti, Aulia Puspita Ardyah Ramadhina Irsanti Putri Aris Sutomo Asri Nurul Huda Aulani&#039;am Aulani&#039;am Aulia Puspita A Yekti Aulia Puspita Anugra Yekti Awang Tri Satria Ayu Melia Sades Dedi Muhammad Dedy Kaunang Effendi, Fredyan Isnan Eko Widodo Eros Sukmawati Ervandi, Mohamad Fahrudin Darlian Gatot Cipatadi Gatot Ciptadi Hanief Eko Sulistyo Hartutik (Hartutik) Hartutik Hartutik Indiah Indiah Indria Susanti Irfan Irfan Khaeruddin Khaeruddin Kuswati Kuswati Ludi Ahmad Jalaludin M Nur Ihsan M. Nur Ihsan M. Pangestu Mashudi Mashudi Moch Sasmito Djati Moh Nur Ihsan Moh. Miftahul Falah Moh. Nur Ihsan Mohammad Nur Ihsan, Mohammad Nur Muhammad Agus Tahar Muhammad Bayuardhi Muhammad Nur Ihsan Muhammad Nur Ihsan Ndaru Pranowo Widiasmoro Nining Haryuni Nisa’us Sholikah Nurgiartiningsih, V. M. A. Nurlayasari, Irma Nurul Isnaini P. Srianto R. P. Putra Rahman Maulana Rahmi, Siti Aulia REZA FAHLEVI Robbaani, Muhammad S. Suyadi Safitri, Brilla Ismaya Saka Wahyu Hikamawan Sepatria Jodiansyah Siti Sunami Sri Rahayu Suhartojo Hardjopranjoto Sutiman Bambang Sumitro Suyadi Suyadi Tri Harsi Trinil Susilawati Trinil Susilawati Trinil Susilawati Veronica Devita Bunga Wulandari, Desinta Y A Tribudi Yanyan Setiawan Yulia Indri Anastasia