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All Journal VALENSI JURNAL MAGISTER HUKUM UDAYANA FIAT JUSTISIA: Jurnal Ilmu Hukum Al Khawarizmi: Jurnal Pendidikan dan Pembelajaran Matematika BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan Jurnal Media Hukum Jurnal Hukum IUS QUIA IUSTUM PADJADJARAN Jurnal Ilmu Hukum (Journal of Law) Jurnal Ilmiah Peuradeun Hasanuddin Law Review SAMARAH: Jurnal Hukum Keluarga dan Hukum Islam Al-Mashlahah: Jurnal Hukum Islam dan Pranata Sosial Jurnal Biologi Tropis EKSAKTA : Jurnal Penelitian dan Pembelajaran MIPA Jurnal Ilmiah Mahasiswa Fakultas Ilmu Sosial & Ilmu Politik Jurnal Hukum Samudra Keadilan BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Jurnal Basicedu EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Media Iuris Biopendix: Jurnal Biologi, Pendidikan dan Terapan Jurnal Pembelajaran dan Biologi Nukleus Best Journal (Biology Education, Sains and Technology) Jurnal Biogenerasi Al-Azkiya : Jurnal Pendidikan MI/SD Science Midwifery At-Tarbawi : Jurnal Pendidikan, Sosial dan Kebudayaan IJELR: International Journal of Education, Language, and Religion Al-Qadha: Jurnal Hukum Islam dan Perundang-Undangan Spizaetus: Jurnal Biologi dan Pendidikan Biologi Jurnal Ilmiah Biologi UMA (JIBIOMA) JL3T (Journal of Linguistics, Literature and Language Teaching) Jurnal Biosense JOLLT Journal of Languages and Language Teaching Bioscientist : Jurnal Ilmiah Biologi Jurnal Ilmiah Mahasiswa Bidang Hukum Kenegaraan Journal of Social and Policy Issues Oryza : Jurnal Pendidikan Biologi Syiah Kuala Law Journal JASL - Journal of Applied Studies in Language Catimore: Jurnal Pengabdian Kepada Masyarakat Jurnal Basicedu LingPoet: Journal of Linguistics and Literary Research Jumper : Journal of Educational Multidisciplinary Research Studies in English Language and Education BIO-CONS: Jurnal Biologi dan Konservasi Kanun: Jurnal Ilmu Hukum Journal of Multidisciplinary Inquiry in Science, Technology and Educational Research Jurnal Ilmiah Mahasiswa Pendidikan Geografi
Zahratul Idami
Fakultas Hukum, Universitas Syiah Kuala
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Journal : Jurnal Biogenerasi

 1 
Hubungan Kekerabatan Kepiting Rajungan (Portunus sp.) Berdasarkan DNA Barcoding di Perairan Percut Sei Tuan Kabupaten Deli Serdang Zuhra, Arini; Manalu, Kartika; Idami, Zahratul
Jurnal Biogenerasi Vol. 10 No. 1 (2024): Volume 10 Nomor 1, Agustus 2024 - Februari 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i1.4834

Abstract

Rajungan crab (Portunus sp.) is one type of crab that has a fairly high commodity value, this species has a fairly wide distribution covering the Indo-Pacific Ocean and also offshore, this crab has an important role in ecological stability. Portunus sp. has a more attractive appearance compared to other crabs. The purpose of this study is to look at the (dominant) crab species found in January (west wind season) in the waters of Percut Sei Tuan based on the genes CO1, and phylogenetics (kinship relationship) of Portunus sp. in the waters of Percut with Portunus sp. in NCBI or Genebank. The method used in this study is DNA isolation on specimens using the Favorgen kit locus of the CO1 gene in the genome of the Portunus sp. amplified by PCR technique with LCO1940 (forward) and HCO2198 (reverse) primers. The results of the research that have been carried out have found 3 species from the genus Portunus, namely P. sanguinolentus, P. trituberculatus and P. pelagicus. Of the three species found in the Percut Sei Tuan Waters, P. trituberculatus and P. pelagicus have a closer kinship relationship compared to P. sanguinolentus, based on the percentage of AT content and GC content, the AT content is higher at 61.03% which means that the three species have primitive properties. The genetic distance between P. sanguinolentus and P. trituberculatus is closer (19.32) compared to P. pelagicus. Based on the phylogenetic tree reconstruction, P. trituberculatus and P. sanguinolentus are closer to P. pelagicus with the Neighbor-Joining method and the Kimura-2-Parameter calculation model. Thus, it can be concluded that DNA barcoding using the CO1 gene locus can be used as a method in identifying Portunus sp. molecularly and kinship relationships with other species.
ANALISIS MOLEKULER KERANG (Anadara sp.) MENGGUNAKAN MARKER GEN CYTOCHROME OXYDASE SUBUNIT 1 (CO1) DI PERAIRAN PERCUT SEI TUAN Dwi Pratiwi, Anggrainy; Idami, Zahratul; Manalu, Kartika
Jurnal Biogenerasi Vol. 10 No. 1 (2024): Volume 10 Nomor 1, Agustus 2024 - Februari 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i1.4835

Abstract

Anadara sp. clams are very popular clams among the community. Anadara sp. also usually live in waters with muddy and sandy surfaces. DNA barcoding is a barcode sequence to identify unknown species. CO1 is a molecular marker commonly used in molecular research on animals. The purpose of this study was to determine the species of Anadara sp. clams found in Percut Sei Tuan Waters using gene markers, the diversity of Anadara sp. clams using CO1 Gene Markers, and the kinship of Anadara sp. clams found in Percut Sei Tuan Waters. This study has 4 stages, namely DNA isolation using the Favorgen kit, PCR amplification, electrophoresis and sequencing. The DNA sequencing results were analyzed using the MEGA 11 application. The results showed that of the three samples that had been analyzed using the CO1 gene marker obtained using BLAST and NCBI, namely the Anadara granosa, Anadara antiquata, and Anadara gubernaculum samples. With the calculation results of higher AT content (28.9%) and lower GC content (20.12%) which states that the species is more primitive. In the pairwise distance matrix, the samples of Anadara antiquata (Percut Sei Tuan) and Anadara granosa (Percut Sei Tuan) which are in the same clade with different sub clades have a genetic distance ranging from 111.45 to 213.77. The phylogenetic tree also shows that the three samples have a close kinship relationship.
IDENTIFIKASI DNA BARCODING TUMBUHAN BALAKKA (Phyllanthus emblica L.) BERDASARKAN LOKUS GEN rbcL Idami, Zahratul
Jurnal Biogenerasi Vol. 10 No. 1 (2024): Volume 10 Nomor 1, Agustus 2024 - Februari 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i1.5145

Abstract

This study aims to identify DNA barcodes with the rbcL gene locus and reconstruct the phylogenetic relationship of the Balakka plant with species belonging to the genus Phyllanthus. This research includes experimental research by isolating DNA, amplification DNA using rbcL gene as primer, electrophoresis and sequencing. DNA was isolated with a commercial kit, amplification was carried out using the Polymerase Chain Reaction method. Sequence results and phylogenetic tree reconstruction were analyzed by Bioinformatics (MEGA 11). The results showed that the number of amplicons from the consensus of the two primers on the balakka plant sample was 557 bp. The phylogenetic tree of the Balakka sequence formed a sister clade with Phyllantus emblica KY9888320.1 with 66% similarity, and P. emblica grouped together, but still had common ancestor branches with other species of the Phyllantus genus using the Neighbor-Joining method, and Kimura 2-parameter calculations.
Co-Authors A. Rani, Faisal Abdul Wahab Abdi, Abdul Wahab Abidin Nurdin Aisyah Hutasuhut, Melfa Alfi Rahman Ali Afwanudin Ali Akbar Alkaf, Muhammad Amalia Mukhtar Ashari, Maya Asri Gani Astuti, Astri Widia Azzahra, Fathia Azzahrawi Azzahrawi Balqish, Annisa Br Sitorus, Julreda Banu Br Tarigan, Rindi Atika Cut Khairunnisak Dakiani diana, Rahma Doly, Raja Dur , Sajaratud Dwi Pratiwi, Anggrainy Eddy Purnama Ellianti, Ellianti Erdiwansyah Erdiwansyah Fanisah Labibah Fatiani Manik Fattara, Fajar Putra Febrian, Fadly Ferdy Saputra Hadi Iskandar Hafsa, Sri Sholeha Harahap, Atika Rahmah Harnita Harnita Hasbi, Yusrizal Hasra, Barratun Nisa Helmi Helmi Husna, Nina Hayatul Husnarika Febriani, Husnarika Husni Djalil Husni Husni Hutasuhut, Melfa Aisyah Ichsan Maulana Idham Idham Ihdi Karim Makinara Ihyannisak Zain Israr Hirdayadi Kasmawati Kasmawati Keumala, Meta Khaira MS, Nazlil Kurniasy, Dessy Lestari, Revi Lubis, Habib Jailani M Ikhsan M. Hardiansyah M. Idris M. Irham M. Zaki Mahfud Mahfud Mahidin Mahidin Mahriza, Rita Manalu, Kartika Manulu, Kartika Mariana Mariana Masturah, Siti Ghaisani Maulidian, Muhammad Okta Ridha Meutia, Cut Intan Muazzin Muazzin muhammad rizky, muhammad Muhammad Tirta Rizky Mukhlis Mukhlis Mukhlis Mukhlis Mumtaz, Raydina Muntiza, Rizka Mursyid Djawas Muslim Zainuddin N. Nazaruddin Nadila, Evi Navisa A, Alya Novianti, Mutiara Nugroho, Muhammad Agung Nurisa, Vina Nurul Fadhilah Pocut Navieza Pratama, Yuda Rizky Puspita, Siti Neni Qudwatin Nisak M. Isa Rahmadina, Rahmadina Rahmah Johar Ramadani, Fitria Ramayati, Ike Rangkuti, Rahmadsyah Rangkuti, Rahmatsyah Raza, Mohd Hamid Rifa'ah, Sirriyatur Riski Afrial Ritonga, Desi Rohaizati, Ulya Rosifa, Gita Dewi Rossya, Nadila Rizki Rusdianda Rusdianda Saifani, Meutia Sajaratud Dur, Sajaratud Salsabila, Nada Shafrida Wati, Shafrida Shafridawati Shella Namira Wardia Shinta Seftyana Silalahi, Ali Affan Simbolon, Wira Hariati Siregar, Elsa Manora Sri Ramayanti Suhaimi Suhaimi Suhery, Dedy Syahputra, Akbar Syahreza, Daffa Rizki Syahrizal Abbas Tambunan, Efrida Pima Sari tiara dewi Utami, Mutiara Alya Wahidah Wahidah Wahidah Wandini, Dwi Febiyanti Yanis Rinaldi Yunita Yunita Yusvita, Diana Zaiyana Putri, Zaiyana Zakaria Zakaria Zuhra, Arini Zulyani Mahmud