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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Biotropika Journal of Innovation and Applied Technology Molecular and Cellular Biomedical Sciences (MCBS) JFMR (Journal of Fisheries and Marine Research) J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Halal Research Bioscientist : Jurnal Ilmiah Biologi Jurnal Gramaswara: Jurnal Pengabdian kepada Masyarakat Jurnal Kefarmasian Indonesia Journal of Coffee and Sustainability
YOGA DWI JATMIKO
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Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Neuroscience Social Sciences Other

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Multidrug-resistant Bacteria in Domestic Cats (Felis catus): A Global Health Threat Emerging from Pets Mumtaza, Furzania; Jatmiko, Yoga Dwi; Salsabila, Maudy Syifa; Faturohmah, Yorda Nur; Fauziah, Ima; Saputra, Sugiyono
The Journal of Experimental Life Science Vol. 16 No. 1 (2026)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2026.016.01.01

Abstract

Cats (Felis catus) act as potential reservoirs for pathogenic bacteria due to their close contact with humans. Despite growing concerns about antimicrobial resistance (AMR) and zoonotic transmission, data on the diversity of antimicrobial-resistant bacteria, especially Staphylococcus aureus, in cats in Indonesia are limited. This study aimed to identify indicator bacteria in domestic cats and characterize their antimicrobial resistance (AMR) profiles. Nine earlobe swab samples were collected from cats at the Splendid Animal Market and Jalan Simpang Madukoro, Malang, in January 2023. White-toyellow colonies with  hemolytic activity formed on blood agar. Six antibiotics are used to asses antimicrobial susceptibility. Based on the European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoints, all isolates (100%) were resistant to rifampicin; seven isolates (78%) to oxacillin and cefoxitin; six isolates (67%) to gentamicin; four isolates (44%) to ceftriaxone; and one isolate (11%) to ciprofloxacin. Seven isolates (P1D, P3, P5, P7, R3, R5, and R9) were  resistance to more than three antibiotic classes were classified as multidrug-resistant bacteria (MDR). Molecular identification using 16S rRNA gene sequencing and phylogenetic analysis revealed the isolates as Chryseobacterium sp. (P1D), Enterobacter hormaechei (P3), Acinetobacter sp. (P5), Enterobacter cloacae (P7), Exiguobacterium sp. (P9A), Staphylococcus sciuri (R1A), Enterococcus hirae (R3), Pantoea sp. (R5), and Stenotrophomonas maltophilia (R9). The detection of ceftriaxone resistance and the presence of MDR bacterial strains indicate that cats carry resistant bacteria that may impact public health.
Characterization of Probiotics Isolated from Intestine of Mackerel Fish (Rastrelliger sp.) from Lembata Regency of East Nusa Tenggara Daten, Helena; Ardyati, Tri; Jatmiko, Yoga Dwi
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.04

Abstract

The research aimed to isolate, characterize, and analyze the ability of lactic acid bacteria (LAB) potential as probiotics to produce hydrolase enzyme. The LAB was isolated using MRS agar by the spread plate method. The LAB characterization includes antimicrobial activity, tolerance to low pH, bile salt, salinity, autoaggregation properties, and ability to produce hydrolytic enzymes. The isolate which has the highest ability to inhibit Aeromonas hydrophila is KBP 3.3, while the isolate which inhibits the highest Streptococcus agalactiae is KBP 1.1.1. The KBP 3.3 and KBP 1.1.1 were able to survive at pH 1 for 24 hours with a survival rate of 93.6% and 98.3%. The KBP 3.3 and KBP 1.1.1 are tolerant to 7.5% bile salt concentrations for 24 hours of 99.46% and 99.11%. The KBP 3.3 is tolerant to 0.5 % salinity for 24 hours with the highest survival rate of 113.38%, while KBP 1.1.1 is 94%. The KBP 3.3 and 1.1.1 have autoaggregation properties of 92.18% and 87.84%. The KBP 3.3 produced the highest lipase enzyme, while KBP 1.1.1 produced the protease enzyme.Keywords: hydrolytic enzyme, lactic acid bacteria, mackerel, probiotic
Antimicrobial Activity of Combination Bacteriocin and Asam Sunti Extract (Averrhoa bilimbi L. fermented) Against Multidrug Resistant Escherichia coli in Lettuces (Lactuca sativa) Kimbal, Angie Via Resty; Jatmiko, Yoga Dwi; Ardyati, Tri
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.06

Abstract

The ready-to-eat vegetables are often associated with the presence of multidrug-resistant (MDR) bacteria. This study aimed to evaluate the potency of bacteriocin, Asam Sunti extract, and their combination against MDR E. coli in lettuce. Their antimicrobial activity was assessed using the disk diffusion method and bacterial enumeration after direct application in pre-inoculated lettuce with MDR E. coli. The bacteriocin was produced by Lactobacillus plantarum BP102 at optimum production time or during the stationary phase at 18 h. These bacteriocins were able to inhibit five MDR E. coli isolates, while Asam Sunti extract and the combination of bacteriocin and Asam Sunti extract were only able to inhibit three MDR E. coli (LL1.2, LL3.11, and LL3.12) and (LL1.2, LL1.3, and LL3.11), respectively. In direct application to pre-inoculated fresh lettuce, higher inhibition of MDR E. coli was observed after applying the combination of bacteriocin and Asam Sunti extract with a ratio of 1:1 and 1:2, compared to bacteriocin alone. However, the inhibitory activity of this combination treatment was not significantly different (p>0.05) with the Asam Sunti extract alone. The highest rate of decrease in total bacteria in lettuces was 97% occurred in isolate LL1.2 with bacteriocin treatment alone, and isolate LL3.11 with combination treatment of bacteriocin and Asam Sunti extract (1:2). While on MCA media, the best reduction rate of 94% occurred in isolate LL1.2 with treatment using bacteriocin only, Asam Sunti extract only, and their combination (1:2). The inhibition of MDR E. coli in fresh lettuces by bacteriocin, Asam Sunti extract, and their combination was strain-dependent which was indicated by various inhibition results in all treatments.Key words: Asam Sunti extract, Bacteriocin, multidrug resistant.
Phenotypic and Genotypic Characterization of Tetracycline-Resistant Bacteria Isolated from Brantas River, Malang Raya and Their Biofilm Formation Ability: Phenotypic and Genotypic Characterization of Tetracycline-Resistant Bacteria Mahira, Syarafin; Yoga Dwi Jatmiko; Maharani Pertiwi Koentjoro
Journal of Tropical Life Science Vol. 16 No. 01 (2026)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.16.01.14

Abstract

Antibiotic resistance in aquatic environments has become an important public health concern due to the spread of antibiotic-resistant bacteria (ARB) through river water, which provides favorable conditions for biofilm formation and enables bacteria to survive environmental stresses, including antibiotic exposure. The Brantas River water is still widely used for household activities, increasing the potential dissemination of bacteria through water exposure. This study aims to determine the phenotypic characteristics, identify tetracycline-resistant bacterial species using 16S rRNA gene sequencing, and analyze the level of biofilm formation and cell density within the biofilm by tetracycline-resistant bacterial isolates. Tetracycline-resistant isolates were phenotypically characterized and identified using 16S rRNA gene sequencing. Detection of tetracycline resistance genes was performed by Polymerase Chain Reaction (PCR). Biofilm formation ability was evaluated using the crystal violet (CV) assay in Tryptic Soy Broth (TSB) and Brantas River water, exopolysaccharide (EPS) extraction, and then the cell density in the biofilm was calculated using Total Plate Count (TPC). Isolates TET-15.1 and TET-40.1 were found to be resistant to all four concentrations. Identification results showed similarities between TET-15.1 with Alcaligenes faecalis; and TET-40.1 with Serratia sp. The resistant genes found in both isolates were tet(S) and tet(B). Alcaligenes faecalis TET-15.1 had the highest biofilm formation ability and higher EPS production than Serratia sp. TET-40.1, with OD584 values ranging from 1.5 to 2.25 and EPS concentration of 4 – 5 mg/mL at tetracycline concentration of 8 μg/mL. The biofilm formation cycle reached maturity after 16 hours of incubation and then dispersed after 32 hours. These findings indicate that tetracycline-resistant bacteria from the Brantas River possess biofilm forming capabilities that may contribute to their environmental persistence and potential role in the dissemination of antibiotic resistance.
Co-Authors Abdul Aziz Jaziri, Abdul Aziz Ahmad Nuril Fuad Al Fatih Al Faizah, Belinda Nabiila Al Fatih, Ahmad Nuril Fuad Al-Faizah, Belinda Nabiila Alviani, Melinda Nuril Ardiansyah, Esha Aris Soewondo Asep Awaludin Prihanto Atho'illah, Mochammad Fitri Atho’illah, Mochammad Fitri Bambang Semedi Bunga Hidayati Endry Nugroho Prasetyo Fadlilah, Dawama Nur Farida Rachmawati, Farida Faturohmah, Yorda Nur Fauziah, Ima Feronika Heppy Sriherfyna Habibah, Laila Nur Harmoko, Deni Helena Daten Ibrahim, Salam A. Ida Ekawati Irfan Mustafa Isdiantoni Izati, Rahmi Jaya Mahar Maligan Kanita, Anggita Bella Siez Kartikaningsih, Hartati Kavitarna, Septhyanti Aprilia Kimbal, Angie Via Resty Maharani Pertiwi Koentjoro Mahira, Syarafin Maulidiyah, Hikmah Melianti, Rika Gian Mochamad Nurcholis Mulasari, Ratna Agista Putri Mumtaza, Furzania Mustamin, Aryan Nashi Widodo Nur, Mokhamad Nurul Afiyatul Jannah Puja, Lintang Ratu Rahayu, Aldila Putri Rahmi Nurdiani Ramadhan, Andi Muhammad Faiz Rifa'i, Muhaimin Rismawati, Ria Sa'adah, Nur Alfi Maghfirotus Sakti, Sefihara Paramitha Salsabila, Maudy Syifa Saputra, Sugiyono Sari, Fikriya Novita Shinta Oktya Wardhani, Shinta Oktya Siti Nur Arifah, Siti Nur SRI RAHAYU Sri Widyarti Suharjono Tri Ardyati Tsuboi, Hideo Widekdo, Dwi purno Wisnu Barlianto Yusuf Efendi