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Arbuscular mycorrhizal fungi (AMF) Glomus spp. as bio-control of Fusarium oxysporum Schlecht et Fr. infection in chilli plants (Capsicum frutescens L.) Ni Putu Muni Raisani; Meitini Wahyuni Proborini; Ni Luh Suriani; Eniek Kriswiyanti
Jurnal Biologi Udayana Vol 24 No 1 (2020): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (536.014 KB) | DOI: 10.24843/JBIOUNUD.2020.v24.i01.p05

Abstract

Fusarium oxysporum Schlecht et Fr. merupakan patogen tular tanah yang dapat menyebabkan layu fusarium pada cabai rawit. Penggunaan Fungi Mikoriza Arbuskula (FMA) sebagai simbion dapat meningkatkan ketahanan tanaman terhadap infeksi jamur pathogen F.oxysporum. Penelitian ini bertujuan untuk mengetahui pengaruh FMA sebagai biokontrol pertumbuhan tanaman cabai rawit terhadap infeksi F. oxysprum serta efektivitas pemberian FMA untuk menurunkan intensitas serangan penyakit layu fusarium. Bibit cabai rawit ditumbuhkan pada media tanah steril (kontrol negatif), tanah steril dan 10 ml patogen (kontrol positif), tanah steril dan FMA 100 g, FMA 100 g dan 10 ml patogen, tanah steril dan 200 g FMA, FMA 200 g dan 10 ml patogen, tanah steril dan 300 g FMA dan 10 ml patogen. Hasil penelitian menunjukkan bahwa pemberian 200 g spora FMA menunjukkan pengaruh berbeda nyata pada tinggi tanaman, jumlah daun, berat kering akar dan berat kering tajuk tanaman cabai rawit berumur tiga bulan. Sedangkan inokulasi 300 g FMA memberikan hasil terbaik pada buah cabai dan persentase kolonisasi FMA sebesar 84, 45%. Inokulasi 300 g FMA menurunkan infeksi F. oxysporum saat 30 Hari Setelah Tanam (HST) sebesar 3.36%. Kesimpulan dari riset ini menunjukkan inokulasi FMA Glomus spp. mampu meningkatkan tinggi tanaman, jumlah daun, berat kering akar dan berat kering tajuk, kecepatan berbunga dan ketahanan tanaman terhadap infeksi jamur F. oxysporum.
KINSHIP ANALYSIS OF GRASS JELLY IN REGENCY OF GIANYAR, TABANAN AND BADUNG BASED ON MORPHOLOGICAL AND ANATOMICAL CHARACTERISTIC Eka Budi Mursafitri; Eniek Kriswiyanti; Pande Ketut Sutara
Jurnal Biologi Udayana Vol 20 No 2 (2016): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Grass jelly is one of the plants that has considerable potential as medicine and drinks. This study was conductedto determine how kinship plant species Grass jelly from three districts. The Exploration of Grass jelly plantsconducted in Gianyar, Tabanan and Badung, Bali province. Making preparations in the Structures Laboratory ofPlant Development (SPT) Faculty Udayana University and the Center of Veterinary (BBVet). This research wascarried out from September 2015-January 2016. This research method using the technique of embedding andfresh slices, FAA fixative (formaldehyde: glacial acetic acid: alcohol 70% = 1: 1: 9), 1% safranin staining in 70%alcohol. For the analysis of kinship qualitative and quantitative data were suspended leaf anatomical charactersto create table Taxonomy Operation Unit (OTU). The results are used OTU table into mini-tab program version14.The result showed that four species of grass jelly plants are Cyclea barbata, Stephania japonica, Stephaniacapitata and Cocculus orbiculatus. Leaf form is like shields and ellipse. Kinship four types of plant grass jellyfrom three districts are very much based on morphological and anatomical characters (level 15.64% similarity).
KARAKTER MORFOLOGI BERAS SEBAGAI PEMBEDA VARIETAS PADI Dicky Frengky Hanas; Eniek Kriswiyanti; I Ketut Junitha
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 7 (2017): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJLFS.2017.v07.i01.p04

Abstract

This study aims to determine the morphological character of white, red and black rice as a differentiator ofrice varieties. This research uses 10 rice samples which are 6 local rice of Kupang, 2 varieties of local superior riceand 2 Italian rice. The method used is direct observation of both qualitative and quantitative characteristics of rice.Long grain size of rice is a character that can indicate the distinguishing of local rice, domestic superior and ricefrom abroad. Character of rice morphology is a character very easily observed in knowing the difference of ricevarieties. Character of rice morphology are easily characters observed in knowing the difference of rice varieties.Characters difference can be used to determine the adulteration of rice mixed.
ANALISA KERAGAMAN GENETIK KELAPA RANGDA (Cocos nucifera L.) DI BALI BERDASARKAN PENANDA DNA MIKROSATELIT Ni Luh Putu Rika Sugiantari; I Ketut Junitha; Eniek Kriswiyanti
SIMBIOSIS Vol 3 No 2 (2015)
Publisher : Jurusan Biologi Universitas Udayana

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Abstract

The research aimed to analyze genetic variation of Rangda coconut in Bali based on microsatellite DNA markers. The young leaf (busung) were collected from Gunaksa village at Klungkung regency, Tulikup village at Gianyar regency, Kubu village at Bangli regency, Sanghyang village, Jembrana regency one sample each, and three samples  are from Ngis village at Karangasem regency. Doyle and Doyle DNA extraction method was used to extract DNA from the samples. Three primer pairs of CnCirC3, CNZ05 and CNZ51 were used to amplify DNA samples. Amplicons were electrophoresed on PAGE 10% and stained with silver nitrate. The allele size was determined by plotting the amplicon migration distance on semilog paper. The results showed there were 11 alleles with an average of 13.33 alleles per primer. There were 3 alleles on primer CNZ05, 4 alleles on primer CnCirC3 and CNZ51 each. Heterozigosity expectation (He) value on primer CnCirC3, CNZ05, and CNZ51   were   0,49±0,04, 0,27±0,03 and 0,71±0,06 consecutively. The result showed that Genetic relationship among 7 coconut samples were divided into two big groups. Key word : Rangda coconut, Microsatellite marker, Number allele, Genetic relationship
PROFIL STRUKTUR SERAT IBU TANGKAI DAUN ANTARA INDUK DAN ANAKAN KELAPA (Cocos nucifera L “Rangda”) Ni Made Puspawati; Eniek Kriswiyanti; I Ketut Junitha
SIMBIOSIS Vol I, No 2, Tahun 2013
Publisher : Jurusan Biologi Universitas Udayana

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The research aims to determine the anatomical structure of the leaf stalk fiber Coconut(Cocos nucifera L. "Rangda") on the seedling and parent plant. Sampling was conducted atSanghyang village Melaya district, Regency of Jembrana. Preparations and microscopicobservations made in the laboratory of Plant Development Structure Biology Department,FMIPA Unud. The research was conducted in October 2012 - April 2013. Maceration methodused for preparations sklerenkim fibers and xylem elements. The results showed that theanatomical parent plant and seedling petiole were torque and mostly composed bysclerenchym fibers but in the parent plant more torque than seedling. In the parent plant about81% the sclerenchym cell was torque but in seedling only 13%, with an average number of17 ± 1 torsion of the parent plant and 6 ± 1 in seedling.Keywords: the fiber structure, torque
MANFAAT TANAMAN TERATAI (Nymphaea sp., Nymphaeaceae) di DESA ADAT SUMAMPAN, KECAMATAN SUKAWATI, KABUPATEN GIANYAR, BALI Gusti Ayu Nyoman Budiwati; Eniek Kriswiyanti
SIMBIOSIS Vol 2 No 1 (2014)
Publisher : Jurusan Biologi Universitas Udayana

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his purpose of this research was to determine the benefits of the lotus plant inSumampan Village, District of Sukawati, Gianyar, Bali. The research was conductedfrom 4 to 16 February 2013. The method was used in this study is exploratory survey bydirect observation and interviews with one main informant and 15 KK from 3 banjar. Theresults showed there were 11 kinds of lotus : lotus sudamala ( Nymphoides indica ) (4.54%), yellow lotus (21.21 %), dark blue lotus ( Nymphaea stellata Wild ) (12.12 %), pinklotus (16.66 % ), violet lotus (9.09 %), purple lotus (9.09 %), white lotus (Nymphaeanouchali Burm f.) (18.18 %), light blue lotus (Nymphaea stellata Wild ) (3.03 %), lotustutur (1.51 %), lotus dedari (1.51 %) and lotus brumbun (3.03 %). The lotus plant is usedas a upakara / banten 77.41 % , 16.12 % as ornamental plants, while 6.45 % as amedicine for breast cancer drug, arthritis, headaches, stress, fear, and cleanser the liverand pancreas. As a medicine, lotus plant was used in it’s from as tempel, solutions, pupukand boreh.Key word: exploratory survey, benefits lotus, tempel, pupuk, boreh
FLORAL DIVERSITY IN MINCIDAN VILLAGE, KLUNGKUNG, BALI TO SUPPORT ECOTOURISM Made Ria Defiani; Eniek Kriswiyanti
SIMBIOSIS Vol 7 No 1 (2019)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (608.8 KB) | DOI: 10.24843/JSIMBIOSIS.2019.v07.i01.p04

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Flora diversity is one of natural resources for living of organism. The study aimed to explore type and usage of plants for medicinal at planned tracking area Mincidan Village to give benefit for education and ecotourism purposes. Method was descriptive analysis by surveying and giving some question to people around the village for plant conservation. Plants that were grown along the tracking site can be grouped as medicinal, ornamental, traditional uses on offerings. Based on the results, the plant can be identified 102 species, 71 genera in 38 families. The plants can be used for material on offering and traditional medicine.
HUBUNGAN KEKERABATAN 12 KULTIVAR BROKOLI (Brassica oleracea L.) BERDASARKAN KARAKTER ANATOMI STOMATA Ni Putu Sri Risa Dewi; Eniek Kriswiyanti; I Gusti Ayu Sugi Wahyuni
SIMBIOSIS Vol 3 No 1 (2015)
Publisher : Jurusan Biologi Universitas Udayana

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This research aims to know the relationship of kinship in 12 cultivars Broccoli grownin Horticultural Nursery at Kembang Merta village Bedugul Bali. Broccoli leaf samples weretaken from Horticultural Nursery at Kembang Merta village Bedugul Bali. The method usedin this research is by utilizing fresh preparations of upside/downside leaf epidermal incisionor Leaf Clearing method, the incision of the preparations and also the observations werecarried out at the Laboratory of Plant Structure and Development Department of Biology,Faculty of Science and Mathematics, Udayana University. The results showed the existenceof variations in epidermal cells, stomata structure, density and density of stomata on the 12cultivars of Broccoli (Brassica oleracea L.) with the range index of similarity between68,79% - 92,18% and formed the five groups that have different similarity indices in eachgroup.Keywords: anatomy, broccoli (Brassica oleracea L.), similarity index of stomata
ANALISIS KEKERABATAN BEBERAPA TANAMAN MANGGA (Mangifera spp.) BERDASARKAN KARAKTERISTIK MORFOLOGI DAN ANATOMI DAUN Luh Putu Melandani; Eniek Kriswiyanti; Made Ria Defiani
SIMBIOSIS Vol 5 No 1 (2017)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (491.332 KB) | DOI: 10.24843/JSIMBIOSIS.2017.v05.i01.p02

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ABSTRACT The study was aimed to explore the characteristics of morphological, anatomical, and phylogenetic relationship between some types of mango (Mangifera sp.). Research conducted in Structures Laboratory of Plant Development Department of Biological Science, University of Udayana from April 2015 - July 2015. Samples were taken from three districts in Bali, that Denpasar, Gianyar and Badung. Ten species of plants of mango (Mangifera indica : ‘poh arum manis’, ‘poh bukit’, ‘poh gedang’, ‘poh madu’, ‘poh apel’,‘poh golek’, ‘poh sambuk’, ‘poh bali’, ‘poh manalagi’) and Wani (Mangifera caesia.) shows the character of morphology and anatomy of leaves varies as well as the analysis of kinship showed ten types of mangoes grouped into two major groups based on the degree of similarity. Fenogram shows the level of similarity of the ten types of mangoes in Bali grouped (cluster) into two: group I ‘poh arum manis’, ‘poh bukit’, ‘poh gedang’, ‘poh bali’, ‘poh apel’, and group II, namely ‘poh madu’, ‘wani’, ‘poh golek’, ‘poh sambuk’, ‘pohmanalagi’ where two clusters have a degree of similarity ? 63.91%.
JENIS - JENIS TUMBUHAN YANG DIGUNAKAN SEBAGAI BAHAN PERAWATAN KECANTIKAN DI PURI DAMAI DESA SINGAKERTA, KECAMATAN UBUD, KABUPATEN GIANYAR Anak Agung Istri Ratih Gayatri; Eniek Kriswiyanti; I Gusti Ayu Sugi Wahyuni
SIMBIOSIS Vol 3 No 1 (2015)
Publisher : Jurusan Biologi Universitas Udayana

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Abstract

Indonesia has many variaties of plant which give a benefit for house, clath, meals,medicine even for beauty treatment. A beauty treatment made from chemical agent causing anegative effect, so searching for an alternative agent for beauty treatment without giving anegative side effect was needed. The goal of has research is to know find species of plants,methods of process also how to process the plants become a herbal beauty treatment agent atPuri Damai which located in Singakerta village, district of ubud, Gianyar region. They takedirect observation, description and identification methods. According to the result of theresearch were found 25 species, 19 suku of plants 3 euphorbiaceae, 2 asteraceae, 2araliaceae, 2 zingiberaceae, pandanaceae, asphodelaceae, poaceae, fabaceae, anonaceae,magnoliaceae, arecaceae, laminaceae, malvaceae, cactaceae, oleaceae, apocynaceae,agavaceae, melastomataceae, rutaceae. Hair vertilizer oil mostly was made from the leaf ofthe plant takes around 56% and for massage oil take the flowers around 53 %. The methodswas taken to produce hair vertilizer oil and ingredients was fried in 1 liter frying oil between6 – 8 hours long in little fire. Direction of use for hair vertilizer oil is rubbing the oil on thecalp and hair before is after washing the hair. The use of massage oil the oli to the body andgive a massage.Keywords : Etnobotany, Beauty treatment, Observation.