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Journal : AGROMIX

Detection and molecular identification of citrus stem rot disease in Bali Province Wirya, Gusti Ngurah Alit Susanta; Agung, I Dewa Agung Putra; Sudiarta, I Putu

Publisher : Fakultas Pertanian Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/agx.v16i1.4075

Abstract

One major disease of citrus is basal stem rot which may cause significant constraint on citrus production in Indonesia. Basically the diseasecan be caused by fungi Phytophthora spp., Diplodia sp., and Botryodiplodia theobromae or Lasiodiplodia theobromae. The purpose of this study wasto provide information on the main pathogens and characterization of BPB disease, especially in citrus in Bali, which ultimately leads to early warning for protection against BPB disease and enriches academic evidence about pre-existing BPB disease.The method used is observation andisolation, pathogenicity test, and in vitro inhibitory test. Research activities include (1) sampling, (2) isolation of pathogens from symptomatic citrusplants, (3) test of pathogenicity, (4) identification of pathogen morphology and molecular, (5) DNA amplification, (6) DNA electrophoresis (7) analysis of DNA sequences. The results of this research successfully identified Lasiodiplodia theobromae, as a pathogen of citrus stem rot disease in Bali Province.
Detection and molecular identification of citrus stem rot disease in Bali Province Wirya, Gusti Ngurah Alit Susanta; Agung, I Dewa Agung Putra; Sudiarta, I Putu
AGROMIX Vol 16 No 1 (2025)
Publisher : Fakultas Pertanian Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/agx.v16i1.4075

Abstract

One major disease of citrus is basal stem rot which may cause significant constraint on citrus production in Indonesia. Basically the diseasecan be caused by fungi Phytophthora spp., Diplodia sp., and Botryodiplodia theobromae or Lasiodiplodia theobromae. The purpose of this study wasto provide information on the main pathogens and characterization of BPB disease, especially in citrus in Bali, which ultimately leads to early warning for protection against BPB disease and enriches academic evidence about pre-existing BPB disease.The method used is observation andisolation, pathogenicity test, and in vitro inhibitory test. Research activities include (1) sampling, (2) isolation of pathogens from symptomatic citrusplants, (3) test of pathogenicity, (4) identification of pathogen morphology and molecular, (5) DNA amplification, (6) DNA electrophoresis (7) analysis of DNA sequences. The results of this research successfully identified Lasiodiplodia theobromae, as a pathogen of citrus stem rot disease in Bali Province.
Co-Authors Abd. Rasyid Syamsuri Agung, I Dewa Agung Putra Anak Agung Istri Kesumadewi ARIYANTA, I PUTU BAWA AStiningsih, Ana Agung Made Chiharu Hongo Damastra, Garda Bagus DEBBIE OKTAVIANI DEPARI Devi, Komang Saraswati Devi, Ni Luh Putu Hartika Sinta Devi, Putu Shinta Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga Dewa Ngurah Suprapta Dharmadiatmika, I Made Agus Dinarkaya, Shah Mahapati DWI WIDANINGSIH Eka Wijayanti, Febri Emilia Simpllisiu Ake Wangge GARGITA, I WAYAN DIKSA Gede Mekse Korri Arisena Gede Suastika Gede Suastika GEDE WIJANA GREGORY C. LUTHER Gunadi, Gusti Alit GUSTI AYU DWITA ANDRAWINA H. Yuswanti Hartha , I Komang Gede Suweca HERRY KUSUMA YUDHA HESTIN YUSWANTI I Dewa Gede Raka Sarjana I Dewa Made Putra Wiratama I Dewa Nyoman Nyana I Dewa Nyoman Nyana I DEWA PUTU SINGARSA I G. R. M. TEMAJA I Gede Agus Adi Chandra I Gusti Agung Oka Hendrawati I GUSTI ALIT GUNADI I Gusti Alit Gunadi I GUSTI AYU ARI SANTIKADEWI I GUSTI AYU DEVI VALENIA SARI I GUSTI AYU KARISMAYATI I GUSTI NGURAH PRABU WIRA SANJAYA I GUSTI NGURAH RAKA I KADEK ARYARTHA I KETUT PURNA YASA I Ketut Siadi I KETUT SUMIARTHA I Komang Candra Giri Prayoga I Made Arimbawa I MADE DEDIK SETYADI I MADE SUDANA I Made Sudana I MADE SUDANA I Made Sudana I Made Sudana I MADE SUDARMA I MADE SUDARMA I MADE SUPARTHA UTAMA I MADE WINANTARA I NENGAH ARTHA I NYOMAN DARMA YASA I NYOMAN RAI I NYOMAN WIJAYA I PUTU BAWA ARIYANTA I Putu Sudiarta I PUTU WIRYA SUPUTRA I Wayan Diara I WAYAN RUSMAN I Wayan Susila I.G.A. Gunadi Ida Ayu Putri Darmawati Ida Bagus Gde, pranatayana JOKO MARIYONO K.A. Yuliadhi K.B. Susrusa KESUMADEWI, ANAK AGUNG ISTRI KETUT AYU YULIADHI Ketut Ayu Yuliadhi KETUT BUDI SUSRUSA Khairun Nisak Syahdu KHAMDAN KALIMI Khamdan Khalimi Klett, Katrina KOMANG ADI MAHARTHA Komang Adi Mahartha Listihani, Listihani Luciana Delavega LUTFI SURYAWAN M SUDANA Made Getas Pudak Wangi MADE MIKA MEGA ASTUTHI Made Satya Andrayuga Masahiro Shishido MEI NOVITA BR PARDEDE Mimi Sutrawati Muhammad Ikhsan Nulzaen N.N.A. Mayadewi NI KADEK NINA ARI SUCI NI KADEK SRI UTARI Ni Komang Budiyani Ni Luh Gde Sumardani NI LUH MADE INDAH MURDYANI DEWI Ni Luh Putu Citra Innosensia NI LUH WAHYU SUTARINI, NI LUH WAHYU NI MADE INDRA PUSPAWATI Ni Made Intan Maulina Ni Made Puspawati NI MADE SAVITA RASJMAN NI MADE TRIGUNASIH NI NENGAH DARMIATI Ni Nengah Putri Adnyani NI NYOMAN ARI MAYADEWI NI NYOMAN DWI RESPITA NINGSIH Ni Putu Merthaningsih Ni Putu Pandawani NI PUTU RATIH SUDIARTINI NI WAYAN SUNITI Nyoman Bintang Kartika Sari Pradana, I Kadek Wira Putra, I Gusti Putu Semara Putu Perdana Kusuma Wiguna Regina I. M BanoEt Retno Kawuri Rindang Dwiyani SANJAYA, I GUSTI NGURAH PRABU WIRA Sarjana, Dewa Gede Raka Selangga, Dewa Gede Wiryangga SHAH KANIGARA ASADDIARI SONIA ASHA HASARI SUGIARTA, DWI SUNARI, ANAK AGUNG AYU AGUNG SRI Suputra , I Putu Wirya TRI ASMIRA DAMAYANTI TRISNA AGUNG PHABIOLA TRISNA AGUNG PHABIOLA UTAMA, I WAYAN EKA KARYA Wibisana, I Made Dicky Chandra WIDHIANTHINI WIDHIANTHINI Wigunanda, I Wayan Surya Aditya Wiraatmaja, Wayan Wulandari, Ni Kadek Pingkan Y. Fitriani YUDHA, I KADEK WISMA Yuliadhi , Ketut Ayu Yuyun Fitriani