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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Media Kedokteran Hewan Veterinary Biomedical and Clinical Journal Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction Bantara Journal of Animal Science
Muhammad Thohawi Elziyad Purnama
Departemen Ilmu Kedokteran Hewan, Fakultas Kedokteran Hewan, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary Other

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Efektivitas Kitosan dari Limbah Kulit Udang terhadap Angiogenesis dalam Penyembuhan Luka Eksisi pada Tikus Putih (Rattus norvegicus) Jantan Dayanti, Efin Windi; Arimbi, Arimbi; Yunita, Maya Nurwartanti; Plumeriastuti, Hani; Purnama, Muhammad Thohawi Elziyad; Wibawati, Prima Ayu
Media Kedokteran Hewan Vol. 32 No. 2 (2021): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v32i2.2021.60-69

Abstract

Luka eksisi adalah luka yang disebabkan akibat terpotongnya jaringan oleh benda yang tajam (Partogi, 2008). Kitosan mampu sebagai zat dalam penyembuhan luka salah satunya dalam proses angiogenesis. Tujuan penelitian ini yaitu untuk mengetahui efektivitas salep kitosan dari limbah kulit udang terhadap angiogenesis dalam penyembuhan luka eksisi pada tikus putih jantan. Sebanyak dua puluh ekor tikus putih (Rattus norvegicus) jantan dibagi ke dalam lima kelompok perlakuan yaitu K+ luka eksisi dengan pemberian povidone iodine, K- luka eksisi dengan pemberian salep tanpa kitosan, P1 luka eksisi dengan pemberian salep kitosan 1,5%, P2 luka eksisi dengan pemberian salep 2,5% dan P3 luka eksisi dengan pemberian salep kitosan. Terapi diberikan sehari sekali selama tujuh hari. Hasil data rata-rata jumlah pembuluh darah baru dianalisis dengan uji Anova yang menunjukkan adanya perbedaan yang signifikan (p<0,05) dan dilanjutkan dengan uji LSD (Least Significant Differences). Kelompok P1 dan P2 tidak memiliki perbedaan yang nyata, tetapi berbeda nyata dengan kelompok K+, K- dan P3. Kesimpulan dari penelitian ini kitosan efektif dalam meningkatkan jumlah pembuluh darah baru pada proses penyembuhan luka eksisi.
A Comparative Histological Study of Skin in Clarias gariepinus and Oreochromis niloticus Khairani, Shafia; Fikri, Faisal; Purnomo, Agus; Purnama, Muhammad Thohawi Elziyad
Media Kedokteran Hewan Vol. 32 No. 2 (2021): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v32i2.2021.90-96

Abstract

This study aimed to evaluate the comparative of histological figures in Clarias gariepinus and Oreochromis niloticus skin. A total of 18 samples were observed and collected the skin preservation. The hematoxylin-eosin staining performed to determine the histological structure. Results showed that there were different structures in the epidermis and dermis layer between C. gariepinus and O. niloticus skin. Club cells showed dominated in the epidermis layer of C. gariepinus. The pigmented cell showed clearly in the basement layer of C. gariepinus compared to O. niloticus. Stratum compactum in O. niloticus also showed adequate compared to C. gariepinus.
Effect of white guava (Psidium guajava L.) fruit juice on the quality of lead acetate induced rats (Rattus norvegicus) spermatozoa Alifia, Annisa; Sri Mulyati; Wurlina Wurlina; Tatik Hernawati; Budi Utomo; Soeharsono Soeharsono; Sri Agus Sudjarwo; Muhammad Thohawi Elziyad Purnama
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 3 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i3.2023.131-140

Abstract

This study aims to determine the effect of the administration of white guava (Psidium guajava L.) fruit juice on spermatozoa plasma membrane integrity (PMI), morphological abnormality, viability, and motility of lead acetate induced rats (Rattus norvegicus). Twenty-five male rats were divided into five groups: NC (negative control) group, rats were administered with distilled water twice daily at four-hour intervals; T0 (positive control) group, rats were administered daily with lead acetate 50 mg/kg bw and distilled water four hours later; T1, T2, and T3 groups, rats were administered daily with lead acetate 50 mg/kg bw and 0.5 mL of 25, 50, and 100% white guava fruit juice four hours later. The treatment of the rats was conducted for 14 days, and on day 15, all rats were sacrificed to assess the spermatozoa quality. Data was analyzed using ANOVA followed by Duncan's multiple range test at a confidence level of 95%. The results showed that exposure to lead acetate (T0) caused lower spermatozoa PMI, viability, and motility as well as higher spermatozoa morphological abnormalities (p <0.05) compared to those of the T0 group. Administration of white guava fruit juice starting at a dose of 25% (T1) resulted in higher spermatozoa motility, viability, and PMI as well as lower spermatozoa morphological abnormalities (p <0.05) compared to rats in the T0 group. It could be concluded that white guava fruit juice maintained the spermatozoa quality of lead acetate induced rats.
Co-Authors Abdul Samik Agus Purnomo Agustono, Bodhi Alifia, Annisa Aliyyah Revinda Bima Putri Arimbi Audina Putri Geraldine BUDI UTOMO Citra Kurnia Putri Dayanti, Efin Windi Dina Deviana Dwi Retna Kumalaningrum Fahlevi Lailiyah Faisal Fikri Fransiska Okta Zania Hani Plumeriastuti Ichi Arum Galerani Imam Mustofa Imas Hapsari Rahmaningtyas Iwan Sahrial Hamid Kenconojati, Hapsari Khairani, Shafia Kuncoro Puguh Santoso Nadiya Listyasari Nove Hidajati Nur Prabowo Dwi Cahyo Praja, Ratih Novita Prastiya, Ragil Angga Santoso, Kuncoro Puguh Saputro, Amung Logam Shabrina Fauzia Prayoga Shabrina Fauzia Prayoga Soeharsono Soeharsono Soeharsono Soeharsono Sri Agus Sudjarwo Sri Mulyati Tatik Hernawati Tri Wahyu Suprayogi Wibawati, Prima Ayu Widya Ayu Kusuma Wisudawati, Feby Dwi Wurlina, W Yudhana, Aditya Yunita, Maya Nurwartanti