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All Journal Jurnal Ilmu dan Teknologi Kelautan Tropis ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Journal of Oceanography Jurnal Pengolahan dan Bioteknologi Hasil Perikanan Journal of Marine Research Jurnal Kelautan Tropis Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology JRST (Jurnal Riset Sains dan Teknologi) Jurnal Intek Akuakultur Jurnal DISPROTEK Pagaruyuang Law Journal Ulumuddin : Jurnal Ilmu-ilmu Keislaman International Journal of Social and Management Studies (IJOSMAS) Indonesian Journal of Mollusk Mukaddimah: Jurnal Studi Islam Analisa: Journal of Social Science and Religion International Journal of Multidisciplinary Sciences and Arts Journal of Fisheries & Marine Jihapenmas Jurnal Ekonomi dan Bisnis Islam
Ali Ridlo
Department Of Marine Sciences, Faculty Of Fisheries And Marine Sciences, Diponegoro University
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Isolasi Glukosamin dari Limbah Cangkang Rajungan Portunus pelagicus (Linnaeus,1758) (Malacostraca:Portunidae) dengan Hidrolisis Asam Klorida Ghofari, Miftahul Akhyar; Ridlo, Ali; Pramesti, Rini
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1548.576 KB) | DOI: 10.14710/jmr.v9i2.26705

Abstract

ABSTRAK: Glukosamin merupakan monomer dari kitosan yang dapat diperoleh dari  limbah  cangkang rajungan (P pelagicus). Glukosamin dibutuhkan  dalam pembentukan dan perbaikan tulang rawan dan jaringan tubuh lainnya. Penelitian ini bertujuan untuk mengetahui karakteristik glukosamin dari limbah cangkang rajungan. Proses isolasi kitosan rajungan terdiri dari deproteinasi dengan NaOH 3N, demineralisasi dengan HCl 1N, dan deasetilasi dengan NaOH 50%. Kitosan yang diperoleh dianalisis karakteristik dan derajat deasetilasinya, selanjutnya kitosan dihidrolisis secara kimia dengan larutan HCl 20% pada suhu kamar selama 4 jam. Glukosamin yang dihasilkan dihitung rendemen, loss on drying (LoD), tingkat kelarutan dan derajat deasetilasinya. Hasil penelitian menunjukan rendemen kitosan cangkang rajungan adalah 11,3%, berwarna putih, tidak berbau, kadar air 9,2%, kadar abu 5,4%, dan derajat deasetilisasi 90,8%. Rendemen glukosamin sebesar 8,6%, dengan nilai Loss on Drying 1,3%, kelarutan sebesar 72% dan derajat deasetilisasi sebesar 96,95%. Spektra infrared menunjukan adanya gugus -NH, -OH, -CH dan –C=O yang sesuai dengan yang terdapat pada glukosamin. ABSTRACT: Glucosamine is a monomer from chitosan which can be obtained from small crab shell (P pelagicus) waste. Glucosamine is needed in the formation and repair of cartilage and other body tissues. This study aims to determine the characteristics of glucosamine from small crab shell waste. The process of isolating chitosan from small crab shells consisted of deproteination with 3N NaOH, demineralization with 1N HCl, and deacetylation with 50% NaOH. The chitosan obtained was analyzed its characteristics and degrees of deacetylation, then chitosan was chemically hydrolyzed with 20% HCl solution at room temperature for 4 hours. The resulting glucosamine is then calculated yield, loss on drying (LoD), solubility level and degree of deacetylation. The results showed that the yield of chitosan crab shells was 11.3%, white, odorless, 9.2% moisture content, 5.4% ash content, and 90.8% deacetylation rate. Glucosamine yield was 8.6%, with a Loss on Drying value of 1.3%, solubility of 72% and the degree of deacetylation of 96.95%. Infrared spectra show the presence of -NH, -OH, -CH and -C = O groups that match those found in glucosamine
Mikroplastik pada Sedimen di Pantai Kartini Kabupaten Jepara Jawa Tengah Azizah, Pramita; Ridlo, Ali; Suryono, Chrisna Adhi
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (678.192 KB) | DOI: 10.14710/jmr.v9i3.28197

Abstract

Microplastic is plastic waste that is less than 5 mm in size and can accumulate in sediments. This study aims to determine the contaminant of microplastics in coastal Kartini sediments, Jepara. Sediment samples were taken in November 2019 with a purposive sampling method using sediment cores at 3 different stations namely the TPI River Estuary (Fish Auction Place), the LPWP River Estuary (Coastal Development Institution), and the BBPBAP River Estuary (Central Brackish Aquaculture Fisheries Center ), at 3 points inside it is 20 cm, 40 cm and 60 cm. The sample is dried and then separated based on grain size using a sieve shaker. Sediments trapped in 0.3 mm sieve size, taken as much as 50 g then immersed in 200 mL 30% H2O2 for 24 hours and then dried. Microplastic is separated from the sediment with 200 ml NaCl ρ = 1.2 g / cm-3, and the remainder the residue is immersed in 200 ml of ZnCl ρ = 1.5 g / cm-3. The number, shape, color and size of the microplastic were observed using the SZ 61 olympus microscope with a magnification of 10 x 10. The results showed that the most microplastics were found in the estuary of the TPI River (Fish Auction Place), namely 643 particles, followed at the estuary of the BBPBAP River (Center for Aquaculture Fisheries) Brackish) 499 particles and at least at station 2 there are 438 particles. The microplastic form is dominated by 506 fragments of particles, while the least microplastic is a pellet that is 295 particles. The microplastic color is dominated by brown as many as 466 particles, while the microplastic color is the least pink and clear as much as 2 particles / 50 g of dry sediment. The largest microplastic size is 208.29 µm and the smallest size is 6.21 µm. ABSTRAK: Mikroplastik adalah sampah plastik yang berukuran kurang dari 5 mm dan dapat terakumulasi pada sedimen. Penelitian ini bertujuan untuk mengetahui kandungan mikroplastik di sedimen pantai Kartini, Jepara. Sampel sedimen diambil pada bulan November 2019 dengan metode purpossive sampling menggunakan sediment core pada 3 stasiun yang berbeda yaitu Muara Sungai TPI(Tempat Pelelangan Ikan), Muara Sungai LPWP(Lembaga Pengembangan Wilayah Pantai), dan Muara Sungai BBPBAP(Balai Besar Perikanan Budidaya Air Payau), pada 3 titik kedalamanyaitu 20 cm, 40 cm, dan 60 cm. Sampel di keringkan kemudian dipisahkan berdasarkan ukuran butir menggunakan sieve shaker. Sedimen yang terjebak dalam sieve ukuran 0,3 mm, diambil sebanyak 50 g kemudian direndam dalam 200 mL H2O2 30% selama 24 jam lalu dikeringkan.Mikroplastik dipisahkan dari sedimen dengan  200 ml NaCl ρ = 1,2 g/cm-3, dan sisa residu nya direndam dalam 200 ml ZnCl ρ =1,5 g/cm-3. Jumlah, bentuk, warna dan ukuran mikroplastik diamati menggunakan mikroskop olympus SZ 61 dengan perbesaran 10 x 10. Hasil penelitian menunjukkan mikroplastik terbanyak ditemukan di Muara Sungai TPI (Tempat Pelelangan Ikan) yaitu 643 partikel/50 g sedimen, diikuti pada Muara Sungai BBPBAP (Balai Besar Perikanan Budidaya Air Payau) 499 partikel/50 g sedimen dan paling sedikit pada stasiun 2 berjumlah 438 partikel/50 g sedimen. Bentuk mikroplastik di dominasi fragmen 506 partikel/50 g sedimen, sedangkan mikroplastik yang paling sedikit adalah pelet yaitu 295 partikel/50 g sedimen. Warna mikroplastik di dominasi warna coklat sebanyak 466 partikel/50 g sedimen, sedangkan warna mikroplastik yang paling sedikit merah muda dan bening sebanyak 2 partikel/50 g sedimen kering. Ukuran mikroplastik terbesar adalah 208,29 µm dan ukuran terkecil adalah 6,21 µm.
Kandungan Mikroplastik pada Sedimen di Perairan Bandengan Kabupaten Kendal Laksono, Ollivia Brylliant; Suprijanto, Jusup; Ridlo, Ali
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v10i2.29032

Abstract

Mikroplastik adalah plastik yang berukuran kurang dari 5 mm sehingga dapat ditrasnportasikan disedimen dan memiliki dampak pada lingkungan. Penelitian ini bertujuan mengetahui bentuk dan jumlah mikroplastik di sedimen Perairan Bandengan Kabupaten Kendal. Sampel sedimen diambil pada Bulan April dan Agustus 2019 secara purposive sampling menggunakan sediment grab pada 3 stasiun berbeda, yaitu stasiun 1 (berjarak ± 9 km dari muara ke laut lepas. Stasiun 2 (berjarak ± 3 km dari muara ke laut lepas dan stasiun 3 (Muara) pada kedalaman lapisan sedimen masing-masing stasiun 2 m. Sedimen sebanyak 25 g direndam dalam 100 ml larutan ZnCl2 (972 g ZnCl2 + 1000 ml akuades) selama 24 jam. Partikel mikroplastik yang mengambang diambil lalu direndam dalam larutan H2O2 30% 50 ml selama 24 jam, kemudian disaring dengan kertas whattman nomor 42 ukuran pori 2 μm dan diamati dengan mikroskop pada perbesaraan 10x10.  Keberadaan mikroplastik di Perairan Bandengan Kabupaten Kendal diduga berasal dari industri disekitar perairan aktivitas nelayan serta kegiatan masyarakat dengan bentuk mikroplastik yang ditemukan yaitu fiber, fragmen, film dan pelet. Jumlah mikroplastik pada Bulan April di stasiun 1, 2 dan 3 berturut turut adalah  271, 142 dan 107 partikel, sedangkan pada Bulan Agustus di Stasiun 1, 2 dan 3  adalah 144, 178, dan 83 partikel. Bentuk mikroplastik yang dominan bervariasi di setiap stasiun pada kedua waktu pengamatan. Pada April, bentuk mikroplastik yang dominan adalah pelet (189 partikel), sedangkan pada Agustus, jumlah terbanyak adalah pelet (186 partikel). Warna yang paling banyak adalah hitam (222 partikel). Ukuran mikroplastik terkecil adalah pelet (1,14 µm) dan terpanjang adalah fiber (214,4 µm).  Microplastics are plastics that are less than 5 mm in size so that they can be transported in sediment and have an impact on the environment. This study aims to determine the shape and amount of microplastics in the sediments of Bandengan waters in Kendal Regency. Sediment samples were taken in April and August 2019 by purposive sampling using sediment grabs at 3 different stations, namely station 1 (± 9 km from the estuary to the open sea. Station 2 (± 3 km from the estuary to the high seas) and station 3 (± 3 km from the estuary to the high seas). Estuary) at a depth of 2 m of sediment layer at each station. 25 g of sediment was immersed in 100 ml of ZnCl2 solution (972 g ZnCl2 + 1000 ml distilled water) for 24 hours. Floating microplastic particles were taken and then immersed in 30% 50 ml H2O2 solution for 24 hours, then filtered with Whattman paper number 42 with a pore size of 2 μm and observed with a microscope at a magnification of 10x10.The presence of microplastics in Bandengan waters, Kendal Regency is thought to have come from industries around the waters of fishing activities as well as community activities with microplastic forms found, namely fiber, fragments , films and pellets. The number of microplastics in April at stations 1, 2 and 3 were 271, 142 and 107 particles, respectively. Shows in August at Stations 1, 2 and 3 are 144, 178, and 83 particles. The dominant form of microplastic varied at each station at the two observation times. In April, the dominant form of microplastic was pellets (189 particles), while in August, the highest number was pellets (186 particles). The most common color was black (222 particles). The smallest microplastic size was pellets (1.14 µm) and the longest was fiber (214.4 µm).
Antibacterial Activities of the Extracts of Sponge-Associated Fungus Trichoderma longibrachiatum against Pathogenic Bacteria Sedjati, Sri; Ambariyanto, Ambariyanto; Trianto, Agus; Supriyantini, Endang; Ridlo, Ali; Bahry, Muhammad Syaifudien; Wismayanti, Gita; Radjasa, ocky Karna; McCauley, Erin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 15, No 2 (2020): August 2020
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v15i2.438

Abstract

This study aims to explore the antibacterial potential of a sponge-associated fungus Trichoderma longibrachiatum isolated from Ternate waters, North Maluku, Eastern Indonesia. Various culture media were used to stimulate the production of secondary metabolites in T. longibrachiatum. The isolate was cultured in various media for 6-9 days. Then, the antibacterial activities of the ethyl acetate extracts were assayed against pathogenic bacteria of Multi-Drug Resistant (MDR) strain (Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae, and Bacillus cereus). The results showed that all extracts had similar profiles on the thin layer chromatography. However, two of the most potent extracts were produced from the PCA and MEA media for 9 days. These extracts inhibited methicillin-resistant S. aureus (MRSA) (12.48 mm and 12.27 mm); B. cereus (12.11 mm and 12.12 mm); K. pneumoniae (12.40 mm and 10.76 mm); and P. aeruginosa (11.59 mm and 8.69 mm) at concentrations 500 mg/disc. In conclusion, the fungus T. longibrachiatum that was cultured in PCA and MEA media had the potential to produce antibacterial compounds against MDR pathogens and both had similar compounds.  Meanwhile, the  ethyl acetate extracts from fungus cultured in the TPA and TA media were inactive against all tested bacteria
Pengolahan Bioplastik Dari Campuran Gliserol, CMC Dan Karagenan Zaky, Muhammad Abdul; Pramesti, Rini; Ridlo, Ali
Journal of Marine Research Vol 10, No 3 (2021): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v10i3.28491

Abstract

Indonesia menghasilkan 64 juta ton sampah di laut dan 3,2 juta ton di antaranya adalah plastik. Pencemaran tersebut mendorong pencarian plastik berbahan dasar ramah lingkungan yang dapat terurai . Bioplastik yang merupakan alternatif kemasan plastik dan bersifat ramah lingkungan. Berbagai bahan dasar pembuatan bioplastik telah ditemukan, salah satunya dasar karagenan. Penelitian ini bertujuan mengetahui karakteristik bioplastik hasil ekstraksi karagenan rumput laut Kappaphycus alvarezii dan mengetahui konsentrasi terbaik bioplastik hasil ekstraksi karagenan berdasarkan tebal film, kuat tarik dan persen pemanjangan. Metode yang digunakan adalah eksperimental laboratoris. Ekstraksi menggunakan perlakuan alkali dengan larutan KOH. Proses ekstraksi menghasilkan tepung karagenan yang digunakan sebagai bahan pembuatan bioplastik. Pembuatan bioplastik menggunakan campuran karagenan dengan 5 variasi massa karagenan, gliserol 10 ml dan 1,2 g CMC. Hasil ekstraksi menghasilkan rendemen 41,12%, kadar air 2,75%, kadar abu 19,10%, kekuatan gel 452,38 dyne/cm2dan viskositas 8,33 cP. Hasil penelitian tentang nilai ketebalan film bioplastik terbaik pada karagenan 3,5 g yaitu 0,093 mm, kuat tarik terbaik pada 1,5 g yaitu 2,587 Mpa, elongasi terbaik pada karagenan 1,5 g sebesar 44,992%. Berdasarkan data tersebut, hasil penelitian ini dapat diaplikasikan sebagai kemasan primer produk pangan. Sea pollution in Indonesia has increased every year with one of the pollutants is plastic. Indonesia produces 64 million tons of waste at sea and 3.2 million tons of which are plastic. The pollution is encouraging researchers to create plastic-based materials that are environmentally friendly and biodegradable. Bioplastics are an environmentally friendly alternative to plastic packaging. This study aims to determine the characteristics of bioplastics extracted from Kappaphycus alvarezii seaweed carrageenan and determine the best concentration of bioplastics from the extraction based on film thickness, tensile strength, and elongation percentage. The method that used in the research is experimental laboratory. The extraction uses alkaline treatment with KOH solution. The extraction process produces carrageenan flour which will be used as a bioplastic material. Making bioplastics using a mixture of carrageenan with 5 variations of concentration, glycerol 10 ml and 1.2 g CMC. The results of extraction showing 41.12% yield, 2.75% moisture content, 19.10% ash content, 452.38 dyne / cm2 gel strength, 8.33 cP viscosity. Carrageenan with the best value of bioplastic film thickness is 3.5 g which is 0.093 mm, the best tensile strength is achieved at 1.5 g with a tensile strength value of 2.587 MPa, the best elongation is achieved at 1.5 g with a value of 44.992%. Based on data showing that this research can be applied as primary packaging for food products.
Karakteristik Krim Tabir Surya dari Kappaphycus alvarezii Doty 1985 (Florideophyceae : Solieriaceae) Beladini, Septi; Susanto, A.B.; Ridlo, Ali
Journal of Marine Research Vol 10, No 3 (2021): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v10i3.31272

Abstract

Paparan sinar ultraviolet yang terus menerus dapat menyebabkan kerusakan pada kulit. Upaya pencegahan dapat dengan menggunakan sediaan krim tabir surya. Rumput laut K. alvarezii adalah salah satu organisme laut yang diduga mengandung senyawa yang dapat dijadikan sebagai agen tabir surya alami. Tujuan dari penelitian ini adalah mengetahui karakteristik krim tabir surya dari ekstrak K. alvarezii. Sampel yang digunakan pada penelitian ini adalah rumput laut K. alvarezii kering yang diperoleh dari PT. Rumah Rumput Laut, Bogor. Rumput laut K. alvarezii diekstraksi dengan pelarut yaitu n-heksana, etil asetat, metanol Krim tabir surya dibuat menurut Mishara, (2018), dengan menambahkan empat ekstrak rumput laut K. alvarezii yang berbeda, kemudian dilakukannya uji yang meliputi uji organoleptik, uji stabilitas, uji tipe emulsi, uji clycling test, uji nilai pH, uji nilai SPF,. Hasil penelitian menunjukkan keempat ekstrak K. alvarezii memiliki serapan panjang gelombang pada daerah ultraviolet, untuk hasil analisis sediaan krim tabir surya menunjukkan krim tabir surya dari ekstrak K. alvarezii stabil secara fisik selama penyimpanan 28 hari, tidak ada perubahan warna dan bau, tidak terdapat pertumbuhan jamur, tipe emulsi  krim minyak dalam air dan memiliki nilai SPF >15 sehingga termasuk dalam kategori tabir surya ultra  Continuous exposure to ultraviolet rays can cause damage to the skin. Prevention efforts can use sunscreen cream preparations.seaweed K. alvarezii is a marine organism that is thought to contain compounds that can be used as natural sunscreen agents. The purpose of this study was to determine the characteristics of the sunscreen cream fromextract K. alvarezii. The sample used in this study was theseaweed K. alvarezii driedobtained from PT. Seaweed House, Bogor.seaweed was K. alvarezii extracted with solvents, namely n-hexane, ethyl acetate, methanol and distilled water. Sunscreen cream made, by adding four seaweed extract K. alvarezii different, then do a test covering the organoleptic test, stability test, test emulsion type,test clyclingtest, test the pH value, test the SPF value, . The results showed that the four extracts of K. alvarezii had wavelength absorption in the ultraviolet area, for the analysis of sunscreen cream preparations showed that the sun cream fromextract K. alvarezii was physically stable during 28 days of storage, no change in color and odor, no growth. fungus, a type of oil-in-water cream emulsion and has an SPF value of> 15 so it is included in the ultra sunscreen category. 
ANTIBACTERIAL ACTIVITY FOR MULTI DRUG RESISTANCE (MDR) BACTERIA BYSEA CUCUMBER Stichopus vastus EXTRACT FROM KARIMUNJAWA ISLANDS – INDONESIA Delianis Pringgenies; Ali Ridlo; Nerva Sembiring
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 9 No. 2 (2017): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (400.552 KB) | DOI: 10.29244/jitkt.v9i2.19302

Abstract

The study aims to explore the antibacterial activity of Stichopus vastus against pathogenic MDR bacteria. Analysis of samples of sea cucumbers included extraction, fractionation, and analysis of bacterial sensitivity test Gas Chromatography-Mass Spectrometry (GC-MS), the extraction process is carried out by solid-liquid extraction method. Fractionation was done with Open-Column Chromatography (OCC). Sensitivity test of bacteria was done using an agar diffusion method according to the Kirby-Bauer (Ref). The study revealed that from 5 species MDR bacteria, which are Coagulant negative stapylococi (CNS), E. coli, Enterobacter 5, Klebsiella sp. dan Pseudomonas sp. There are two MDR bacteria had the most sensitive responses by the extract of sea cucumber, which were Enterobacter-5 and Klebsiella sp. The two bacteria were tested against five bioactive fractions obtained from OCC. Fraction criteria-2 had the highest antibacterial activity against Enterobacter-5 and Klebsiella sp, with serial concentration of 20 µg ∙ disc–1, 40 µg. disc–1 and 80 µg. disc–1.  Largest inhibition zone were obtained from 80 µg. disc–1 againts the two bacteria were (14.73 ± 0.48) mm and (11.22 ± 0.85) mm respectively.  GC-MS Analysis revealed that fraction criteria-2 had (or consisted of) cyclohexhane, ethanol, butanoic and pentanoic acids. Keywords: antibacterial activity, multi drug resistance (MDR), sea cucumber, Stichopus vastus
Aplikasi Ekstrak Rumput Laut Sebagai Agen Imunostimulan Sistem Pertahanan Non Spesifik Pada Udang (Litopennaeus vannamei) Ali Ridlo; Rini Pramesti
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 14, No 3 (2009): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (411.171 KB) | DOI: 10.14710/ik.ijms.14.3.133-137

Abstract

Penelitian ini bertujuan untuk menganalisis potensi ekstrak dari rumput laut Dictyota sp., Gracilaria sp., Padina sp., dan Sargassum sp. sebagai imunostimulan sistem pertahanan tubuh non spesifik udang L. vannamei. Rumput laut diekstraksi dengan cara direbus dalam air mendidih selama 2 jam. Suplementasi ekstrak pada pakan udang buatan diberikan dengan dosis 10 g/kg pakan. Hasil penelitian menunjukkan adanya peningkatan jumlah hemosit dan aktivitas fagositosis udang L. vannamei akibat pemberian ekstrak rumput laut dibandingkan kontrol. Peningkatan tersebut terlihat pada  jumlah hemosit udang L. vannamei  yang diberi ekstrak Gracilaria sp. pada hari ke-8 , Dictyota  sp. dan Sargassum sp pada hari ke 12,  sedangkan  peningkatan aktifitas fagositosis terjadi pada perlakuan dengan ekstrak Dictyota sp., Gracilaria sp. dan Sargassum sp. pada hari ke-8. Pemberian ekstrak Sargassum sp memberikan hasil terbaik yaitu untuk jumlah hemosit 1,127±0,260 pada hari ke-12 dan aktifitas fagositosis 214,061±15,955 pada hari ke- 8. Kata kunci : Imunostimulan, rumput laut, L. vannamei Study on application of seaweed Dictyota sp., Gracilaria sp., Padina sp. and Sargassum sp. extract as imunostimulant agent for non-specific defense system of white shrimp (L. vannamei) was conducted. Air - dried seaweed was extracted by boiling in water for 2 hours then fortified into shrimp feed pellet with concentration of 10g/kg pellet. Haematological parameters of Total Haemocyte Count (THC) and Phagocytosist Activity (PA) was then tested at 4, 8, 12 days period after initial treatment. The result showed that fortification of seaweed extract was increased the number of haemocyte was clearly shown at day 8 of Gracilaria sp. extract treatment and day 12 of Dictyota sp. and Sargassum sp. treatment. Meanwhile phagocytosist activity was clearly increased at day 8 of Dictyota sp., Gracilaria sp. and Sargassum sp treatment.  Extract  of Sargassum sp. gave  the most significant effect with total haemocyte of 1,127 ± 0,260 at day 12th and phagocytosist activity of 214,061± 15,955 at day 8th. Key words : Immunostimulant, seaweed, L. vannamei
Potensi Berbagai Jenis Lamun Sebagai Sumber Makanan Kesehatan : Analisis Proksimat Wilis A Setyati; Subagiyo Su8bagiyo; Ali Ridlo
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 10, No 2 (2005): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.10.2.115-118

Abstract

Penelitian ini bertujuan untuk mempelajari status nutrisi berbagai jenis lamun yang terdapat diperairanBandengan, Jepara berdasarkan analisis proksimat (gula reduksi, serat kasar, lemak, protein dan abu). Penelitian ini juha melakukan analisis vegetasi lamun di perairan Bandengan, Jepara. Hasil penelitian menunjukan ada 6 jenis lamun yang tumbuh di perairan Bandengan Jepara yaitu Cymodecea serrulata, Thalassia hemprichii, Enhalus acoroides, Syrirgodium isoetifolium, Halodule uninervis dan Thalassodendron ciliatum. Keenam jenis lamun tersebut berturut-turut mempunyai kepadatan 109,00 ind/m2, 106,87 ind/m2, 89,35 ind/m2 , 82,97ind/m2 , 73,65 ind/m2 dan 18,75 ind/m2. Hasil analisis proksimat utuk ke enam jenis lamun tersebut berturut turut adalah sebagai berikut, Cymodecea serrulata mempunyai kandungan protein, gula reduksi, lemak, abu dan serat kasar berturut-turut (% berat kering) 9,39 ; 0,91; 7,81; 67,09; 19,25, Thalassia hemprichii berturut turut 8,35; 1,10 ; 7,38; 62,43; dan 17,27, Enhalus acoroides berturut-turut 7,65 ; 1,00; 6,13 ; 68,14 ; dan 19,92, Syrngodium isoetifolium berturut-turut 5,52 ; 2,19; 4,71; 70,62 dan 12,16. Sedangkan Halodule uninervis dan Thalassodendron ciliatum tidak dilakukan analisis proksimat karena jumlah vegetasi yang adasangat sedikit.Kata kunci : Lamun, analisis proksimat, analisis vegetasiThe research was done to answer how about nutritive profile (protein, lipids, reduction sugar ash and crudefibre) of some seagrass and how about some seagrasses can be improved potentially as foodhealthness. In this research vegetaion analyze or seagrasses also was done, to identified rheir diversity and density. The research found 6 species of seagrass in seagrass filed of Bandengan waters, Jepara, i.e. Cymodecea serrulata, Thalassia  hemprichii, Enhalus acoroides, Syrirgodium isoetifolium, Halodule uninervis dan Thalassodendron ciliatum. There have population density respectively 109,00 ind/m2, 106,87 ind/m2, 89,35 ind/m2 , 82,97 ind/m2, 73,65 ind/m2 and 18,75 ind/m2.The result of proximate analyze showed that Cymodecea serrulata haveprotein , sugar reduction, lipid, ash and crude fibre (% dry weight) respectively 9,39 ; 0,91; 7,81; 67,09;19,25, Thalassia hemprichii 8,35; 1,10 ; 7,38; 62,43; dan 17,27, Enhalus acoroides 7,65; 1,00; 6,13; 68,14 ; and 19,92, Syrngodium isoetifolium 5,52 ; 2,19; 4,71; 70,62 and 12,16. Halodule uninervis and Thalassodendron  ciliatum have not beeb abalyzed.Key words: seagrass, proximate analysis, vegetation analysis.
Uji Bioaktivitas Ekstrak Batang Tumbuhan Benalu Mangrove (C. filiformis) : I. Uji Antifungal Subagiyo Subagiyo; Wilis A Setyati; Ali Ridlo
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 9, No 4 (2004): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.142 KB) | DOI: 10.14710/ik.ijms.9.4.180-185

Abstract

Benalu adalah tumbuhan epifit parasit yang hidup menempel dan menghisap makanan dari tumbuhan inangnya. Selama proses penyerapan makanan ini akan ikut pula semua senyawa yang dibentuk oleh mangrove sebagai alat pertahanan diri, maka tumbuhan benalu yang hidup pada tumbuhan mangrove juga mempunyai kemampuan khusus untuk beradaptasi terhadap senyawa-senyawa yang dibentuk oleh tumbuhan mangrove. Sehingga dimungkinkan untuk ditemukannya jenis-jenis senyawa baru yang mempunyai potensi anti fungi. Senyawa-senyawa aktif ini kemudian dapat dikembangkan lebih lanjut dalam rangka pengendalian penyakit baik bagi manusia maupun dalam bidang budidaya perikanan, peternakan maupun pertanian. Penelitian dilakukan dengan metode experimental. Ekstrak kasar diperoleh dengan metode masrasi menggunakan methanol sebagai pelarutnya. Sedangkan fraksinasi dilakukan dengan metode kromatografi kolom menggunakan methanol-chloroform (20:1) sebagai eluennya. Aktivitas antifungal diuji dengan metode paper disk. Ada 5 variasi konsentrasi fraksi yang diuji yaitu 50 ug/disk, 10 ug/disk, 5 ug/disk, 1 ug/disk dan 0,5 ug/disk. Hasil studi parasit-host menunjukan bahwa dilokasi penelitian (kawasan mangrove Teluk Awur-Jepara) C. filiformis terdapat sebagai parasit pada tumbuhan mangrove Lumnitzera sp dan Excoecoria aggaloca. Sedangkan hasil uji aktivitas antijamur menunjukan bahwa semua fraksi menunjukanaktivitas antijamur terhadap jamur Trichoderma hanya pada pengamatan 24 jam pertama. Aktivitas anti jamur terhadap Fusarium ditunjukan oleh semua fraksi, dan aktivitas terhadap Aspergillus ditunjukan olehfraksi fraksi 1 dan fraksi 5.Kata kunci : bioaktivitas, antifungal, extract batang, C filiformis, benalu mangroveMistletoe as arboreal parasitic plant, hold and absorb their nutrient from host. During nutrient absorption, all of metabolites (include secondary metabolites) which host producing will be absorbed too. For thatmistletoe must have capability to developing strategies to adapt. The one of adaptation strategies is creating bioactive compounds, and predicted that between bioactive compounds have antifungal activity.Extraction and fractination of stem of C. filiformis, and testing of its antifungal activity were done. Stem of C. filiformis was extracted in methanol. Crude extract was fractionated chromatograhically using methanolchloroform (20:1) as eluent. Antifungal was tested using agar disc-diffusion methods. There are 5 variation concentration, which tested, 50 ug/disc, 10 ug/disc, 5 ug/disc, 1 ug/disc and 0,5 ug/disc. Theresult showed that C. filiformis found as parasite on Lumnitzera sp and Excoecoria aggaloca. Antifungal test toward C. filiformis fractions showed that Fusarium and Trichoderma were inhibited by all of fractionswhile Aspergillus was inhibited only by fraction 1 and fraction 5.Key words : bioactivity, antifungal, stem extract, C. filiformis, misletoe
Co-Authors A. Budi Susanto A.B. Susanto A.B. Susanto Adi Santosa Agus Sabdono Agus Trianto Agustin, Nurul Ahmad Saddam Habibi Ainul Fithriyah Akbar, Muhamad Rahadian Alfin Anggraeni Ali Djunaedi Ambariyanto , Ambariyanto Ambariyanto Amtoni Caesario Nainggolan Anggi Setiabudi Anisah Harahap Anita Dwi Kartika Annisa Afifah Nugroho Anthony D Arnanda Antonius Budi Susanto Arahmad, Yulianto Puguh Arif Maa’ruf Al Ayyub Arifah, Dewi Arifin, Muhammad Sholeh Arvianto Wibowo Azhar, Nuril Azizah, Pramita Bahry, Muhammad Syaifudien Bambang Yulianto Bara Yanwar Hadi Nugroho, Bara Yanwar Beladini, Septi Chrisna Adhi Suryono Christtenson Purba Christtenson Purba Clara Vania Cornelia Widya Seprandita Dafit Ariyanto Delianis Pringgenies Dewi Kartika Sari Dewi, Lutfianna Fatma Dinda Ayuniar Zanjabila Dion Ragil Saputra Dion Ragil Saputra Dodik S. Wicaksono Dwi Haryo Ismunarti Edi Wibowo Efendi, Muhammad Luthfi Eka Mulya Eko Sasmaya Eko Sasmaya Endang Supriyantini Endang Supriyantini Erma Nurmalitasari Ervia Yudiati Esayani Rosadi Faishal Islami Faishal Islami, Faishal Faiz Naida Salimah Ghofari, Miftahul Akhyar Gunawan Widi Santosa Gunawan Widi Santosa Gunawan Widi Santoso Hafida Salma Hakim, M Sabron Sukmanul Harahap, Akbar Ibnu Pratikto Ibnu Wardani Iin Putriyani Irwani Irwani Ita Widowati Ivend Umbu Jawa Ivend Umbu Jawa, Ivend Umbu Jelita Rahma Hidayati Jusup Suprijanto Jusup Suprijanto Jusup Suprijanto Juwita Lesly Senduk Khusnul Khotimah Kirana Fatika Brilianti Koesoemadji Koesoemadji Laksono, Ollivia Brylliant Langit, Novita Thea Puspita Lilik Maslukah Linggar Dirgantara Prasetyo Luluk Abdatur Rosyidah Hanum Lutfianna Fatma Dewi Mahadika Fanindhita Sany Manggola, Alen Mardani Mardani Masri, Mohammed Sharin Haji McCauley, Erin Miftahul Akhyar Ghofari Mohamad Mirza Mohamad Mirza Muhamad Rahadian Akbar Muhamad Rahadian Akbar Muhammad Abdul Zaky Muhammad S. Bahry Muhammad Zainuddin Nada Kristiani Ginting Nada Salsabila Nadiah Humairoh Mufidah Savitri Nadya Oktavia Nerva Sembiring Nirwani Soenardjo Novita Thea Puspita Langit Nur Arif Kurniawan Nur Indah Febriani Nur Indah Febriani Nurul Agustin Ocky Karna Radjasa Oetari Kusuma Putri Ollivia Brylliant Laksono Pramastuti, Fransisca Ria Pramita Azizah Pratiwi, Siswantar Putri, Dhiya Aflah Luswanto Rachman, Rhima Rismiyati Raden Ario Raditya Ahmad Rifandi Radityo Haris Rahmadika, Reza Ratna Ibrahim Refi Sekarwardhani Retno Hartati Revo Raprika Kurniawan Rhima Rismiyati Rachman Ribka Anindita Br Perangin-angin Rifa’i, Akhmad RINI PRAMESTI Rini Pramesti Rizky Rifatma Jezzi Rizqi Umi Arifah Roestiawan, Dwi Bagus Salma, Hafida Salsabila, Nada Santoso, Gunawan Widi Savitri, Nadiah Humairoh Mufidah Sedjati, Sri Sedjati Septi Beladini Setia Devi Kurniasih Setia Devi Kurniasih, Setia Devi Setiyorini, Alin Sri Sedjati Sri Sedjati Sri Yulina Wulandari Subagiyo Subagiyo Subagiyo Subagiyo Subagiyo Subagiyo Sulistiana, Zalsabila Sunaryo Sunaryo Sunaryo Sunaryo Sunaryo Sunaryo Suryono Suryono Syafaatul Laili Syamsu Dluha Tri Aji Pamungkas Utomo, Wahyu Laurentius Pria Victorina Yulina Santi Wahyu Laurentius Pria Utomo Wardani, Ibnu Widianingsih Widianingsih Wilis A Setyati Wilis A Setyati Wilis A Setyati Wilis Ari Setyati Wilis Ari Setyati Wismayanti, Gita Yohanes Oktaviaris Zaenal Arifin Zahari, Abdul Kadir Mulku Zaky, Muhammad Abdul Zalsabila Sulistiana