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Actinomycetes from Plant Rhizosphere in Gorontalo Karst Area as Plant Growth Promoting Rhizobacteria Matalauni, Citra Leonita; Retnowati, Yuliana; Katili, Abubakar Sidik; Kandowangko, Novri Youla; Hasan, Ani M.
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1047-1053

Abstract

This study aimed to describe Actinomycetes from the rhizosphere of plants in the Gorontalo karst area as Plant Growth Promoting Rhizobacteria (PGPR). The research method is based on a quantitative descriptive method. Soil samples from the rhizosphere were collected using a purposive sampling technique from 8 plant species at two locations in the Gorontalo karst region, namely the Tanjung Kramat Hills. The characteristics of PGPR in this study focused on phosphate solubilization ability, Indole Acetic Acid (IAA) production, antagonism against the fungus Fusarium oxysporum, and tolerance to fungicides. Potential PGPR isolates were analyzed for phylogenetic relationships based on 16S rRNA gene sequences. The results showed that there were 6 actinomycetes isolates (RzHKC-01, RzKO-02, RzO-03, RzAK-04, RzPK-05, and RzOC-06) from 8 plant species in the Gorontalo karst region. One actinomycetes isolate, RzPK-05, showed potential as a PGPR with the ability to solubilize phosphate and produce IAA at 0.165 mg/L and 0.133 mg/L, respectively. Isolate RzPK-05 exhibited antagonistic properties against Fusarium oxysporum with an inhibition rate of 82.24% and was tolerant to fungicides such as Captive, Benlate, and Thiramo up to a concentration of 100 mg/L. Molecular analysis based on the 16S rRNA gene and phylogenetic tree reconstruction indicated that the RzPK-05 isolate is closely related to the genus Streptomyces with 100% similarity.
Analisis Proksimat Jagung Pulut (Zea mays L. var Damahu) dari Desa Bontula Kecamatan Asparaga Kabupaten Gorontalo Mustamin Ibrahim; Devi Bunga Pagalla; Abubakar Sidik Katili; Nurul Fajryani Usman
Jurnal Riset Rumpun Ilmu Tanaman Vol. 4 No. 2 (2025): Oktober : JURRIT: Jurnal Riset Rumpun Ilmu Tanaman
Publisher : Pusat riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jurrit.v4i2.7254

Abstract

The Damahu waxy corn (Zea mays L. var. Damahu) is a local variety traditionally cultivated by the Gorontalo community and possesses significant potential for the development of functional food products. This study aimed to determine the nutritional composition of the Damahu waxy corn variety originating from Bontula Village, Asparaga District, Gorontalo Regency, through proximate analysis. The corn samples were dried, milled, and analyzed using standard analytical procedures to quantify moisture, ash, fat, protein, and carbohydrate contents. The results indicated that Damahu waxy corn flour contained 83.32% carbohydrates, 5.93% protein, 1.67% fat, 8.84% moisture, and 0.24% ash. This composition characterizes the Damahu variety as a good energy source. However, its protein content does not yet meet the minimum requirement set by the Indonesian National Standard (SNI) for corn-based processed products. The nutritional profile highlights the potential of Damahu waxy corn for the development of nutritious and competitive local food products. This study provides an initial contribution to the documentation of Gorontalo’s indigenous corn varieties and offers a scientific foundation for the utilization of local germplasm to strengthen regional food security.
Research-Based Teaching E-Modules on Mangrove Diversity to Enhance Meaningful Learning in Banggai Regency Haruna, Moh. Fahri; Fahru, Almira Rayyah Shadriah; S, Siti Marwah; Katili, Abubakar Sidik; Odja, Abdul Haris
Jurnal Penelitian Pendidikan IPA Vol 11 No 11 (2025): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i11.13084

Abstract

The importance of mangrove ecosystems such as shoreline buffers, biodiversity habitats, and carbon sinks is increasingly threatened by anthropogenic degradation. This study aimed to develop and validate a meaningful learning–based e-module relevant to the context of mangrove diversity in Banggai Regency. Mangrove diversity was utilized as teaching material in the developed e-learning module to provide students with early knowledge about mangroves, enabling them to protect and conserve mangrove ecosystems in their environment. The method employed was a three-stage Research and Development (R&D) approach: field exploration, e-module development based on field data and meaningful learning principles, and product validation by subject matter experts, media experts, and practitioners through observations and questionnaires. Data were analyzed using descriptive qualitative and quantitative methods. The results showed that this interactive e-module, through a limited trial involving 30 students, achieved an average feasibility score of 89.7%. The discussion confirmed that the application of meaningful learning principles integrated with local contexts proved highly valid and effective in improving students' understanding of mangrove diversity. In conclusion, this e-module is feasible and can be implemented to support conservation efforts and enhance students’ understanding. (The total word count is approximately 145, which is concise and covers all essential elements).
E-Modul Based on Educational Tourism at Kilo 5 Luwuk Beach: Development of Learning Material to Faster Environmental Awareness in Junior High School Student Haruna, Moh. Fahri; Abdan, Hasna; Yusuf, Frida Maryati; Katili, Abubakar Sidik
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 4: Jurnal Pembelajaran Dan Biologi Nukleus December 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i4.8357

Abstract

Background: This research was initiated in response to the noticeable lack of environmental awareness among students in coastal regions, despite the area's abundant natural resources, and the absence of innovative, contextually relevant science learning tools. The research aims to develop a digital edutourism-based e-module at Kilo 5 Beach in Luwuk, designed to enhance students' conceptual understanding and foster environmental appreciation. Methodology: The research employs a Research and Development (R&D) approach, specifically the 4D development model (define, design, develop, and disseminate), though only three stages were executed: a preliminary study, design and validation by subject matter and media experts, and effectiveness testing with educators and students. Effectiveness was evaluated using pre- and post-tests, with data analyzed through the N-Gain formula and t-tests. Findings: This e-module is validated as educational resource. Expert validation confirmed the e-module's validity, with average scores exceeding 9%, indicating no revisions were needed. Practicality assessments revealed positive feedback from both teachers and students, with scores in the excellent category (above 90%), demonstrating the e-module's high practicality. The e-module's effectiveness was further evidenced by an increase in students' understanding, reflected in an N-Gain value of 0.6316 (medium category) and a significant t-test result (0.00<0.05). Contributions: This research provides empirical support for the use of educational tourism–based e-modules as contextual digital learning tools to improve environmental awareness and conceptual understanding among coastal junior high school students. It advances the 4D development framework by embedding local ecotourism contexts into science learning materials and evaluating their validity, practicality, and learning impact. The findings offer a transferable instructional approach that links formal science education with local environmental resources to support place-based and sustainability-focused learning.