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All Journal International Journal of Public Health Science (IJPHS) ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kesehatan Lingkungan indonesia Jurnal Ilmu Dasar e-Journal Pustaka Kesehatan Jurnal Kesehatan Masyarakat Althea Medical Journal Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Majalah Geografi Indonesia Jurnal Kesehatan Jurnal Kesehatan Andalas Folia Medica Indonesiana Damianus Journal of Medicine Journal of Agromedicine and Medical Sciences Jurnal Media Gizi Indonesia (MGI) JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) Majalah Kedokteran Sriwijaya Universa Medicina Jurnal Bioteknologi & Biosains Indonesia (JBBI) Journal Of Vocational Health Studies Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan Al-sihah: The Public Health Science Journal Jurnal Medik Veteriner SRIWIJAYA JOURNAL OF MEDICINE Journal of Health Sciences JHECDs: Journal of Health Epidemiology and Communicable Diseases JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Berita Kedokteran Masyarakat JEBIMAN: Jurnal Ekonomi, Bisnis, Manajemen dan Akuntansi Jember Medical Journal (JMJ) Jurnal Ners dan Kebidanan (Journal of Ners and Midwifery) ASPIRATOR - Journal of Vector-Borne Diseases Studies
Bagus Hermansyah
Departemen Parasitologi, Fakultas Kedokteran Universitas Jember, Jalan Kalimantan 37 Kampus Tegal Boto, Jember, Jawa Timur 68121
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Library & Information Science Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Journal : Jurnal Bioteknologi

 1 
PERAN PROTEIN PILI 11 kDa Streptococcus pneumoniae SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN Mufida, Diana Chusna; Salsabila, Yuna Annisa; Suswati, Enny; Hermansyah, Bagus; Agustina, Dini
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v7i1.3930

Abstract

Role of Pili Protein 11 kDa of Streptococcus pneumoniae as Hemagglutinin and Adhesin Protein Streptococcus pneumoniae has pili which play roles in adhesion, colonization of nasopharyngeal epithelial cells, and phagocytic inhibition of immune cells. This study aimed to determine the characteristics of the 11 kDa pili protein as hemagglutinin and adhesin, as well as their immune responses. The 11 kDa pili protein from S. pneumoniae was isolated by SDS-PAGE, purified by electroelution and dialysis. Hemagglutination and adhesion tests were carried out on the protein, and western blotting of the polyclonal antibody immune responses were evaluated. Hemagglutination test showed that the 11 kDa pili protein played a role in the hemagglutination process up to 2-time dilution. Adhesion test showed there was a correlation between the dose of the protein and the bacteria attached to the epithelial cells. The Pearson correlation test showed a P value of 0.010 and a correlation coefficient of R = -90.919. Quadratic regression test produced R2 = 0.974. Western blotting test showed that 11 kDa pili protein polyclonal antibodies recognized 67 kDa and 11 kDa pili proteins. The study concluded that the 11 kDa S. pneumoniae pili protein acted as hemagglutinin and adhesin, and the polyclonal antibody protein responded to 67 pDa and 11 kDa BM pili proteins.Keywords: adhesin, hemagglutinin, pili, protein 11 kDa, Streptococcus pneumoniae ABSTRAKStreptococcus pneumoniae memiliki pili yang berperan dalam adhesi, kolonisasi sel epitel nasofaring, serta sebagai inhibitor fagositosis sel imun. Penelitian ini bertujuan mengetahui karakteristik protein pili 11 kDa sebagai hemagglutinin dan adhesin serta respons imunnya. Protein pili 11 kDa dari bakteri S. pneumoniae diisolasi secara SDS-PAGE, dipurifikasi dengan elektroelusi dan dialysis. Uji hemaglutinasi dan adhesi dilakukan pada protein tersebut, serta dievaluasi respon imun poliklonal antibodinya secara western blotting. Uji hemaglutinasi menunjukkan protein pili 11 kDa berperan dalam proses hemaglutinasi hingga pengenceran 2 kali. Uji adhesi menunjukkan korelasi antara dosis protein dan bakteri yang menempel pada sel epitel. Uji korelasi Pearson menunjukkan P value 0,010 dan koefisien korelasi R = -0,919. Uji regresi Quadratic menghasilkan R2 = 0,974. Uji Western blotting menunjukkan antibodi poliklonal protein pili 11 kDa mengenali protein pili 67 kDa dan 11 kDa. Penelitian ini berkesimpulan protein pili 11 kDa S. pneumoniae berperan sebagai hemaglutinin dan adhesin, serta antibodi poliklonal protein tersebut memberi respons terhadap protein pili BM 67 kDa dan 11 kDa. 
PERAN PROTEIN PILI 11 kDa Streptococcus pneumoniae SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN Diana Chusna Mufida; Yuna Annisa Salsabila; Enny Suswati; Bagus Hermansyah; Dini Agustina
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v7i1.3930

Abstract

Role of Pili Protein 11 kDa of Streptococcus pneumoniae as Hemagglutinin and Adhesin Protein Streptococcus pneumoniae has pili which play roles in adhesion, colonization of nasopharyngeal epithelial cells, and phagocytic inhibition of immune cells. This study aimed to determine the characteristics of the 11 kDa pili protein as hemagglutinin and adhesin, as well as their immune responses. The 11 kDa pili protein from S. pneumoniae was isolated by SDS-PAGE, purified by electroelution and dialysis. Hemagglutination and adhesion tests were carried out on the protein, and western blotting of the polyclonal antibody immune responses were evaluated. Hemagglutination test showed that the 11 kDa pili protein played a role in the hemagglutination process up to 2-time dilution. Adhesion test showed there was a correlation between the dose of the protein and the bacteria attached to the epithelial cells. The Pearson correlation test showed a P value of 0.010 and a correlation coefficient of R = -90.919. Quadratic regression test produced R2 = 0.974. Western blotting test showed that 11 kDa pili protein polyclonal antibodies recognized 67 kDa and 11 kDa pili proteins. The study concluded that the 11 kDa S. pneumoniae pili protein acted as hemagglutinin and adhesin, and the polyclonal antibody protein responded to 67 pDa and 11 kDa BM pili proteins.Keywords: adhesin, hemagglutinin, pili, protein 11 kDa, Streptococcus pneumoniae ABSTRAKStreptococcus pneumoniae memiliki pili yang berperan dalam adhesi, kolonisasi sel epitel nasofaring, serta sebagai inhibitor fagositosis sel imun. Penelitian ini bertujuan mengetahui karakteristik protein pili 11 kDa sebagai hemagglutinin dan adhesin serta respons imunnya. Protein pili 11 kDa dari bakteri S. pneumoniae diisolasi secara SDS-PAGE, dipurifikasi dengan elektroelusi dan dialysis. Uji hemaglutinasi dan adhesi dilakukan pada protein tersebut, serta dievaluasi respon imun poliklonal antibodinya secara western blotting. Uji hemaglutinasi menunjukkan protein pili 11 kDa berperan dalam proses hemaglutinasi hingga pengenceran 2 kali. Uji adhesi menunjukkan korelasi antara dosis protein dan bakteri yang menempel pada sel epitel. Uji korelasi Pearson menunjukkan P value 0,010 dan koefisien korelasi R = -0,919. Uji regresi Quadratic menghasilkan R2 = 0,974. Uji Western blotting menunjukkan antibodi poliklonal protein pili 11 kDa mengenali protein pili 67 kDa dan 11 kDa. Penelitian ini berkesimpulan protein pili 11 kDa S. pneumoniae berperan sebagai hemaglutinin dan adhesin, serta antibodi poliklonal protein tersebut memberi respons terhadap protein pili BM 67 kDa dan 11 kDa. 
Co-Authors Achmad Maududie Achmad Nazalal Furqon Adilah Julinar Irianti Afifatun Hasanah Agustina Tri Endharti Ahmad Hasbi Al-Muzaky Alfianti, Nita Angga Mardro Raharjo Ardyansyah, Rizky Robeth Arifin, Mochammad Samsul Asis Fitriana Ayundasari, Asti Azham Purwandhono Aziz, Ayu Munawaroh Billy Jusup Kurniawan Brillian Adexa Yudinda Burhan, Niniek Cholis Abrori, Cholis Cicih Komariyah Desi Dwi Cahyani Diana Chusna Mufida Dicky Setiawan Didi Candradikusuma Didi Candradikusuma Dina Helianti Dinda Ayu Wanodya Supriatiningsih Dini Agustina Drajad, Rahadinda Mutia Dhamar Dwita Aryadina Dwita Aryadina Rachmawati Dwita Sakuntala Edda Rachmadenawanti El Milla, Kardiana Izza Ellen Ocktavironita Elvia Rahmi Marga Putri Enny Suswanti ENNY SUSWATI Eny Nurmaida Epafroditus Sanjaya Adiguna Erfan Efendi Erma Sulistyaningsih Erma Sulistyaningsih Febrianti, Zahrah Fitri, Nadya Eka Ghaiska Najma Amnur Habib Mustofa Habibi, Ali Hanu Neda Septian Heni Fatmawati Hidayat Sujuti Ida Srisurani Wiji Astuti Ika Rahmawati Sutejo Indra Samudra Rahmat Irawan Fajar Kusuma Ivan Iqbal Baidowi Jauhani, M. Afiful Jauhar Firdaus Josef Sem Berth Tuda Kesy Sasta Handani Kristianningrum Dian Sofiana Kusiyanto Taslim, Fitranda Lailatis Shofia Laili, Nikmatul Laily Nurhayati Laksmi Indreswari Laksmi Indreswari Leersia Yusi Ratnawati Loeki Enggar Fitri M. Ali Shodikin M.S. Irham Rozaq Made Masagung Kawiartha Mash’ab Zirrun Al-Qomah Muhammad Afiful Jauhani Muhammad Alif Taryafi Muhammad Dimas Arya Candra Permana Muhammad Ryznar Faisal Nur Luqmani Nabila Putri Anissa Nexia Nevarachell Onny Amirsyah Nidya Husna Kholidah Niniek Burhan Nita Alfianti Nugraha, Bima Setia Sandya Nurmaida, Eny Nurul Furqooniyah Pangestu, Ahmad Yudho Hadi Pralampita, Pulong Wijang Primadana, Aditya Putra, Muhamad Dwi Eka Putri, Elvia Rahmi Marga Qintar Layallia Faza Rahardjo, Angga Mardro Rahmadhan, Adiz Dwiputra Rahmi, Aulya Raissa Puan Andrina Ramadhani, Rafindra Rena Normasari Rezza Putri Mahartika Risyanto, Chusnul Aulia Arisny Putri Sa'bani, Begawan Sakinah, Elly N Salsabila, Yuna Annisa Salsabilla, Safira Putri Nur Samsul Arifin Saputra, Antonius Dwi Sarah Andriani Selly Silfana Liensa septa surya wahyudi, septa surya Shabri, Hammam Arif Shodikin, M Ali Shodikin, M. Ali Shofia, Lailatis Siti Zulaikha Sri Rahmanita Sugiyanta Sugiyanta Supangat Supangat Syamsuddin, Alif Fazduani Tiara Ajeng Dewita Tristianti, Farmitalia Nisa Tuda, Josef Sem Berth Tutiana Bayati Umar Zein Wiwien Sugih Utami Wiwin Sugih Utami Yoga Setyo Yonas Hadisubroto Yudha Nurdian Yuli Hermansyah Yuli Hermansyah Yuli Rahmantia, Vania Yuna Annisa Salsabila Yunita Armianti Yunita Armiyanti Zahrah Febianti Zulaikha Rizqina Rahmawati