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All Journal International Journal of Public Health Science (IJPHS) ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kesehatan Lingkungan indonesia Jurnal Ilmu Dasar e-Journal Pustaka Kesehatan Jurnal Kesehatan Masyarakat Althea Medical Journal Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Majalah Geografi Indonesia Jurnal Kesehatan Jurnal Kesehatan Andalas Damianus Journal of Medicine Journal of Agromedicine and Medical Sciences Jurnal Media Gizi Indonesia (MGI) JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) Majalah Kedokteran Sriwijaya Universa Medicina Jurnal Bioteknologi & Biosains Indonesia (JBBI) Journal Of Vocational Health Studies Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan Al-sihah: The Public Health Science Journal Jurnal Medik Veteriner SRIWIJAYA JOURNAL OF MEDICINE Journal of Health Sciences JHECDs: Journal of Health Epidemiology and Communicable Diseases JOURNAL OF COMMUNITY MEDICINE AND PUBLIC HEALTH RESEARCH JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Berita Kedokteran Masyarakat JEBIMAN: Jurnal Ekonomi, Bisnis, Manajemen dan Akuntansi Jember Medical Journal (JMJ) Jurnal Ners dan Kebidanan (Journal of Ners and Midwifery) ASPIRATOR - Journal of Vector-Borne Diseases Studies Folia Medica Indonesiana
Bagus Hermansyah
Departemen Parasitologi, Fakultas Kedokteran Universitas Jember, Jalan Kalimantan 37 Kampus Tegal Boto, Jember, Jawa Timur 68121
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Library & Information Science Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Sensitivity and Specificity of Nested PCR for Diagnosing Malaria: Cases in Several Areas of Indonesia Arifin, Samsul; Fitri, Loeki Enggar; Sujuti, Hidayat; Hermansyah, Bagus; Endharti, Agustina Tri; Burhan, Niniek; Candradikusuma, Didi; Sulistyaningsih, Erma; Tuda, Josef Sem Berth; Zein, Umar
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.02.11

Abstract

Indonesia is still included in high endemic area of malaria infection. Early detection as well as appropriate and quick treatment is needed to be able to prevent and treat malaria in Indonesia. Laboratory examination using a microscopic method is still used as the gold standard to diagnose malaria cases. However, the morphology similarity of some Plasmodium species and the number of parasites that can be seen under microscopy causes malaria diagnosis become difficult if only relying on microscopy diagnostic method. The purpose of this study is to analyze the sensitivity and specificity of nested PCR compared to microscopic examination in diagnosing malaria cases. A cross-sectional study has been carried out in some areas of Indonesia and the microscopic analysis as well as nest PCR was done in Laboratory of Parasitology and Laboratory of Central Biomedical Faculty of Medicine, Universitas Brawijaya, Malang East Java Indonesia. A total of 149 blood samples from patients with clinical symptoms of malaria had been obtained from Sumatra, Sulawesi and East Java during December 2011 to December 2013. From 149 sample, 81.9% samples were diagnosed malaria positive by microscopy examination, whereas the PCR results showed that 90.6% of samples were positive. Nested PCR sensitivity is 97.5%, and microscopy 88.2%. Nested PCR specificity is 40.7%, whereas microscopy 78.5%. PPV and NPV for nested PCR are 88,2% and 78.5% respectively, and for microscopy are 97.5% and 40.7% respectively. Nested PCR has a higher sensitivity than microscopy in diagnosing malaria and is able to detect mixed infection better than microscopic examination. However, it is statistically less specific than microscopy examination.
Sensitivity and Specificity of Nested PCR for Diagnosing Malaria: Cases in Several Areas of Indonesia Samsul Arifin; Loeki Enggar Fitri; Hidayat Sujuti; Bagus Hermansyah; Agustina Tri Endharti; Niniek Burhan; Didi Candradikusuma; Erma Sulistyaningsih; Josef Sem Berth Tuda; Umar Zein
Journal of Tropical Life Science Vol. 8 No. 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.02.11

Abstract

Indonesia is still included in high endemic area of malaria infection. Early detection as well as appropriate and quick treatment is needed to be able to prevent and treat malaria in Indonesia. Laboratory examination using a microscopic method is still used as the gold standard to diagnose malaria cases. However, the morphology similarity of some Plasmodium species and the number of parasites that can be seen under microscopy causes malaria diagnosis become difficult if only relying on microscopy diagnostic method. The purpose of this study is to analyze the sensitivity and specificity of nested PCR compared to microscopic examination in diagnosing malaria cases. A cross-sectional study has been carried out in some areas of Indonesia and the microscopic analysis as well as nest PCR was done in Laboratory of Parasitology and Laboratory of Central Biomedical Faculty of Medicine, Universitas Brawijaya, Malang East Java Indonesia. A total of 149 blood samples from patients with clinical symptoms of malaria had been obtained from Sumatra, Sulawesi and East Java during December 2011 to December 2013. From 149 sample, 81.9% samples were diagnosed malaria positive by microscopy examination, whereas the PCR results showed that 90.6% of samples were positive. Nested PCR sensitivity is 97.5%, and microscopy 88.2%. Nested PCR specificity is 40.7%, whereas microscopy 78.5%. PPV and NPV for nested PCR are 88,2% and 78.5% respectively, and for microscopy are 97.5% and 40.7% respectively. Nested PCR has a higher sensitivity than microscopy in diagnosing malaria and is able to detect mixed infection better than microscopic examination. However, it is statistically less specific than microscopy examination.
Co-Authors Achmad Maududie Achmad Nazalal Furqon Adilah Julinar Irianti Afifatun Hasanah Agustina Tri Endharti Ahmad Hasbi Al-Muzaky Angga Mardro Raharjo Ardyansyah, Rizky Robeth Arifin, Mochammad Samsul Asis Fitriana Ayundasari, Asti Azham Purwandhono Aziz, Ayu Munawaroh Billy Jusup Kurniawan Brillian Adexa Yudinda Burhan, Niniek Cholis Abrori, Cholis Cicih Komariyah Desi Dwi Cahyani Diana Chusna Mufida Dicky Setiawan Didi Candradikusuma Didi Candradikusuma Dina Helianti Dinda Ayu Wanodya Supriatiningsih Dini Agustina Drajad, Rahadinda Mutia Dhamar Dwita Aryadina Dwita Aryadina Rachmawati Dwita Sakuntala Edda Rachmadenawanti El Milla, Kardiana Izza Ellen Ocktavironita Elvia Rahmi Marga Putri Enny Suswanti ENNY SUSWATI Eny Nurmaida Epafroditus Sanjaya Adiguna Erfan Efendi Erma Sulistyaningsih Erma Sulistyaningsih Febrianti, Zahrah Fitri, Nadya Eka Ghaiska Najma Amnur Habib Mustofa Habibi, Ali Hanu Neda Septian Heni Fatmawati Hidayat Sujuti Ida Srisurani Wiji Astuti Ika Rahmawati Sutejo Indra Samudra Rahmat Irawan Fajar Kusuma Ivan Iqbal Baidowi Jauhani, M. Afiful Jauhar Firdaus Josef Sem Berth Tuda Kesy Sasta Handani Kristianningrum Dian Sofiana Kusiyanto Taslim, Fitranda Lailatis Shofia Laili, Nikmatul Laily Nurhayati Laksmi Indreswari Laksmi Indreswari Leersia Yusi Ratnawati Loeki Enggar Fitri M. Ali Shodikin M.S. Irham Rozaq Made Masagung Kawiartha Mash’ab Zirrun Al-Qomah Muhammad Afiful Jauhani Muhammad Alif Taryafi Muhammad Dimas Arya Candra Permana Muhammad Ryznar Faisal Nur Luqmani Nabila Putri Anissa Nexia Nevarachell Onny Amirsyah Nidya Husna Kholidah Niniek Burhan Nita Alfianti Nugraha, Bima Setia Sandya Nurmaida, Eny Nurul Furqooniyah Pangestu, Ahmad Yudho Hadi Pralampita, Pulong Wijang Primadana, Aditya Putra, Muhamad Dwi Eka Putri, Elvia Rahmi Marga Qintar Layallia Faza Rahardjo, Angga Mardro Rahmadhan, Adiz Dwiputra Rahmi, Aulya Raissa Puan Andrina Ramadanti, Novi Awalia Ramadhani, Rafindra Rena Normasari Rezza Putri Mahartika Risyanto, Chusnul Aulia Arisny Putri Rozaq, M.S. Irham Sa'bani, Begawan Sakinah, Elly N Salsabila, Yuna Annisa Salsabilla, Safira Putri Nur Samsul Arifin Saputra, Antonius Dwi Sarah Andriani Selly Silfana Liensa septa surya wahyudi, septa surya Shabri, Hammam Arif Shodikin, M Ali Shodikin, M. Ali Shofia, Lailatis Siti Zulaikha Sri Rahmanita Sugiyanta Sugiyanta Supangat Supangat Syamsuddin, Alif Fazduani Tiara Ajeng Dewita Tristianti, Farmitalia Nisa Tuda, Josef Sem Berth Tutiana Bayati Umar Zein Wiwien Sugih Utami Wiwin Sugih Utami Wulandari, Lindi Priyantika Tri Yoga Setyo Yonas Hadisubroto Yudha Nurdian Yuli Hermansyah Yuli Hermansyah Yuli Rahmantia, Vania Yuna Annisa Salsabila Yunita Armianti Yunita Armiyanti Zahrah Febianti Zulaikha Rizqina Rahmawati