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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA Jurnal Ilmu Lingkungan Jurnal Akademika Biologi International Journal of Marine and Aquatic Resource Conservation and Co-existence Prosiding KPSDA Jurnal Kelautan Tropis E-Dimas: Jurnal Pengabdian kepada Masyarakat Jurnal Bioteknologi & Biosains Indonesia (JBBI) Biosaintifika: Journal of Biology & Biology Education Journal of Applied Food Technology Berkala Bioteknologi NICHE Journal of Tropical Biology Scripta Biologica Cendekia Journal of Pharmacy Jurnal Pasopati : Pengabdian Masyarakat dan Inovasi Pengembangan Teknologi Jurnal Abdimas Ilmiah Citra Bakti (JAICB) Prosiding Konferensi Nasional PKM-CSR Makara Journal of Science Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Hermin Pancasakti Kusumaningrum
Biotechnology Study Program , Biology Department, Faculty Of Sains And Natural Sciences, Diponegoro University
Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Journal : Scripta Biologica

 1 
CHARACTERIZATION OF 18S RIBOSOMAL RNA FRAGMENT FROM Solanum tuberosum L. var. Granola POTATO Yunior William Susanto; Hermin Pancasakti Kusumaningrum; Siti Nur Jannah; Sri Rustini
Scripta Biologica Vol 5, No 1 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (979.814 KB) | DOI: 10.20884/1.sb.2018.5.1.760

Abstract

Potato (Solanum tuberosum L.) is a prime horticultural commodity. One of the varieties of potato that widely cultivated in Indonesia is Granola. This study characterized the variety Granola based on the 18S sequences of rRNA gene fragment. The 18S sequences were used to distinguish the Granola and determine the differentiating characters from other Solanum based on those sequences data. The characterization was completed in three main steps including DNA isolation from potato leaf using Doyle & Doyle method, amplification of the 18S gene fragment, and DNA sequencing. The amplification of 18S gene fragment by a PCR method obtained 528 bp sequences. The BLAST search using NCBI web service confirmed that Granola potato has 99% matching sequence with S. tuberosum. The phylogenetic reconstruction further indicates the S. tuberosum var. Granola used in this study deeply nested with the reference sequence X67238.1, a potato from Europe.
Co-Authors Agung Suprihadi Agung Suprihadi Agus Sabdono Agus Setiadi Agus Subagio Anggoro, Naufal Sebastian Annisa Fadillah Annisa Nur Ayuningtyas Anto Budiharjo Arina Tri Lunggani Aris Ismanto Asih Rismiarti, Asih Athried Elsima Azis Rifai Baskoro Rochaddi Bintoro Rudi Saputro, Bintoro Rudi Budi Raharjo Chatarina Umbul Wahyuni Choirunnisaa, Nadia Maharani Jasmine Christina Ratna Handayani, Christina Ratna Christina Retna Handayani, Christina Retna Clara Yully Diana Ekaristi Denis Denny Nugroho Sugianto Dinda Khairunnisa, Dinda Doktorasaintifika, Heradita Kaniaazzahra Dudi Hardianto, Dudi Eiffeliena Nur'aini Fisikaningputri Purwienanti Eko Purnomo Elke Gildantia Elsima, Athried Endang Dwi Purbajanti Endang Kusdiyantini Erfianti, Tia Fiona Aqhila Dewi Fitri Arum Sasi, Fitri Arum Furgeva, Natasha Gentur Handoyo Hadi Endrawati Heksa Raihan Hererapratita Aysha Hapsari Herida, Azalia Puspa Hersugondo Hersugondo Hersugondo Hersugondo Herusugondo Herusugondo Imam Misbach Imam Mishbach Indah Sulistyarini Indras Marhaendrajaya Is Helianti IS HELIANTI Istiana, Rohma Ita Widowati Izzudin, Maulana Zidan Jihadi, Muhammad Shulhan Joedono Soedarsono Joedoro Sudarsono Karima, Radhita Kurniawati, Mufida Budi Lilis Sugiarti Lilis Sugiarti Lina Mulyawati Maria, Atina Mawarni, Shelina Nurunnisa Maya Aresteria MG Isworo Rukmi Monalita, Ramadhebi Muhammad Amal Nurhakim, Muhammad Amal Muhammad Anwar Djaelani Muhammad Iskandar Zulkarnain Muhammad Iskandar Zulkarnain Muhammad Mulyadi, Muhammad Muhammad Zainuri Mulyawati, Lina Natasha Furgeva Nurhayati Nurhayati Nurhayati Nurhayati Okti Hajeng Kristiadi Rahayu, Annisa Rizky Rejeki Siti Ferniah Retno Hartati Risma Wiharyanti Rizko, Nurmalisa Robertus Triaji Mahendrajaya Roslenawati Roslenawati Rudhi Pribadi Safirah, Dearesty Sarwo Edi Wibowo, Sarwo Edi Siti Nur Jannah Siti Nur Jannah Solly Aryza Sonny Abdi Setiyawan, Sonny Abdi Sri Pujiyanto Sri Pujiyanto Sri Pujiyanto Sri Pujiyanto Sri Rustini Subagiyo Subagiyo Sulis Setyowati, Sulis Suryani, Oda Gracia Ariela Sutrisno Anggoro Tony Hadibarata, Tony Tri Rahayu, Hesti Triwibowo Yuwono Wahyu Dewi Utari Haryanti Wahyuningsih, Candra Widianingsih Widianingsih Wijanarka Wijanarka Yann Hardivillier Yason Lukman Sudjito, Yason Lukman Yosef Purwoko Yoshua Mario Sumbodo Yudi Yunanto Yumna Rahmadias Hanifa Yunior William Susanto Yuvita Muliastuti