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Journal : Biologica Samudra

Diversity of Endophytic Fungi in The Twigs and Roots of Baccaurea brevipes Hook.f. in Langsa Protected Forest, Aceh, Indonesia Wibowo, Sara Gustia; Navia, Zidni Ilman; Harmawan, Tisna; Dwitya, Yonadiah; Lestari, Ratna; Fadhliani, Fadhliani
JURNAL BIOLOGICA SAMUDRA Vol 7 No 1 (2025): Biologica Samudra
Publisher : Program Studi Biologi, Fakultas Teknik, Universitas Samudra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jbs.v7i1.10991

Abstract

The Baccaurea brevipes Hook.F. plant has been studied in the past and has the potential to be a therapeutic herb. The Tampoi plant (B. brevipes) has been used as a medication by the Acehnese to regulate menstruation and make urinating easier. In nature, endophytic fungi are widespread and can be easily found in plant tissue. Without infecting the host plant, the endophytes colonize the inter and intracellular spaces of the tissue. Endophytic fungi can create bioactive chemicals that may be similar to or distinct from those of their host plants and have pharmacological effects. However, no studies have been done on its endophytic fungus as a potential source for novel medication development. This study was done to look into endophytic fungi in Baccaurea's twigs and roots. The techniques employed in this study were endophytic fungi purification, identification of macroscopic and microscopic endophytic fungi, and endophytic fungi isolation. Five isolates were identified as a result of isolation and purification; they were designated as isolates BVA1, BVA2, BVA3, BVR1 and BVR2. The genera Neofusicocum sp., Colletotrichum sp., Mucor sp., Fusarium sp. and Schizophyllum sp. are assumed to be the kind of endophytic fungal isolates BVA1, BVA2, BVA3, BVR1 and BVR2.
Uji efektivitas kombinasi ekstrak etanol daun mangkokan (Nothopanax scutellarium Merr.) dan daun sirih (Piper betle L.) sebagai ovisida alami terhadap telur Aedes aegypti vektor demam berdarah dengue Wibowo, Sara Gustia; Lestari, Ratna; Sari, Herlina Putri Endah
JURNAL BIOLOGICA SAMUDRA Vol 6 No 1 (2024): Biologica Samudra
Publisher : Program Studi Biologi, Fakultas Teknik, Universitas Samudra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jbs.v6i1.10293

Abstract

Aedes aegypti merupakan salah satu serangga vektor penyebab utama penyebaran virus dengue ke manusia. Pengendalian biasa dilakukan dengan penanggulangan vector. Salah satunya dengan penggunaan ovisida kimia. Penggunaan ovisida kimia dalam jangka waktu panjang beresiko mencemari lingkungan. Oleh karena itu, penting untuk mencari alternatif ovisida alami dalam pengendalian vektor yaitu penggunaan daun sirih (Piper betle L) dan daun mangkokan (Nothopanax scutellarium Merr.). Tujuan dari peneltian ini untuk mengetahui efektivitas dari kombinasi ekstrak daun sirih dan daun mangkokan sebagai salah satu bahan baku ovisida. Penelitian menggunakan rancangan acak lengkap dengan konsentrasi ekstrak 1%, 1,5%, 2%, 2,5% (v/v). Kontrol negatif dan positif menggunakan aquades dan bubuk abate. Data kemudian dianalisis menggunakan ANOVA melalui uji BNT dan analisis Probit untuk menentukan nilai LC50. Hasil dari penelitian ini menunjukkan bahwa kombinasi ekstrak daun sirih dan ekstrak daun mangkokan efektif sebagai ovisida alami telur Ae. aegypti pada konsentrasi 2% (v/v).