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INDUKSIVARIASI SOMAKLONAL JARAK PAGAR (JATROPHA CURCAS L.) UNTUK MENDAPATKAN KARAKTERTOLERAN CEKAMAN KEKERINGAN ., Maftuchah
Jurnal Gamma Vol 6, No 1 (2010): September
Publisher : Jurnal Gamma

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Experimental activities is conduct in several stages, that is : somaclonal variation induction incastor oil in vitro culture through the use of PEG and selected plantlet is reproduce in vitro. In vitroselection procedure is done by planting cotyledon explants from castor oil seedlings age 10-14 daysin nutrient medium MS+BAP+IBA. Seed sterilization is done using Tween 20, Benlate, 90% alcohol,50% chloroc and sterile water.After callus is grown, callus will be sub-cultured into nutrient mediumMS+BAP with PEG addition. Selected callus will be transferred into nutrient medium MS + potassiumpantotenat + active coal until it shows root growth.Result of experiment has shown that the higher PEG concentration will suppress thegrowth of castor oil callus. In PEG concentration 15% the mortality rate of castor oil callus (LD) is64,29% and for PEG concentration 20% the mortality rate of castor oil callus is 92,86%. For thenext phase is in vitro selection process through treatment of 15% PEG toward all seven accessions.Result of this experiment has revealed that average mortality rate of callus in 15% PEG treatment is87,64%. HS-49 has shown the lowest percentage rate of response (79%) in 15% PEG treatment,while the highest percentage rate of response occurs for SP-8 (93%). Callus that succeeding growthfrom PEG selection medium will be reproduce in regeneration medium until bud is formed. Buddingconstruct percentage is range between 28,57% (accession HS-49 and SP-38) until 37,03% (accessionSM-35). Average budding construct percentage from selected callus in regeneration medium is31,45%. For the next stage of experiment (second year), those bud will be sub-cultured into rootingmedium until forming root and become complete plantlet ready to acclimatize.

TRANSFORMASI ANGGREK DENDROBIUM DENGAN GEN GUS-A MELALUI PERANTARAAN AGROBACTERIUM TUMEFACIENS MAFTUCHAH, .
Jurnal Gamma Vol 1, No 2 (2006): Maret
Publisher : Jurnal Gamma

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Serangkaian penelitian telah dilakukan untuk mendapatkan tanaman anggrek Dendrobium transgenik dengan mengintroduksikan gen gus-A ke protochorm like bodies dengan menggunakan teknik Agrobacterium tumefaciens EHA 105 dan plasmid vektor pCambia 1303. Hasil penelitian menunjukkan bahwa jumlah protochorm like bodies hidup tertinggi pada media antibiotik (VW + 250 mg/l cefotaxime dan VW + 100 mg/l cefotaxime) diperoleh pada perlakuan periode ko-kultivasi selama 1 hari, dimana persentase PLB hidup pada media VW + 100 mg/l cefotaxime mencapai 82%. Peningkakan lama periode ko-kultivasi pada proses transformasi genetik terbukti meningkatkan persentase kematian PLB. Pada penelitian ini, perlakuan periode ko-kultivasi 2 hari dan 3 hari menghasilkan ekspresi gen gus-A tertinggi pada PLB anggrek Dendrobium, dimana persentase PLB positif gen gus-A mencapai 100%. Tetapi, periode ko-kultivasi 3 hari meningkatkan PLB kontaminasi Agrobacterium tumefaciens (46%) dan menurunkan persentase PLB hidup anggrek Dendrobium (30%) pada media VW + 250 mg/l cefotaxime.

PENGEMBANGAN METODE ISOLASI DNA GENOM PADA TANAMAN JARAK PAGAR (JATROPHA CURCAS) Maftuchah, Agus Zainudin,
Jurnal Gamma Vol 2, No 1 (2006): September
Publisher : Jurnal Gamma

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Molecular markers have been used extensively to study genetic relationships in number of crops. One of the techniques that can be used to obtain DNA finger print is RAPD (random amplified polymorphic DNA) technique, a molecular technique based on PCR technology. The quality of amplification products depend on several factors such as MgCl2 concentration, primers, PCR condition and DNA quality. In this study, an experiment was conducted to find out appropriate DNA isolation technique for three accessions Jatropha curcas (Karangtengah, Lamongan and NTB). Three DNA isolation technique has been tested and two of them were able to produce good quality of genomic DNA. The results therefore indicate that the technique can be used to genomic DNA isolation of Jatropha curcas on molecular analysis.

TRANSFORMASI ANGGREK DENDROBIUM DENGAN GEN gus-A MELALUI PERANTARAAN Agrobacterium tumefaciens . MAFTUCHAH
Jurnal Gamma Vol. 1 No. 2 (2006): Maret
Publisher : Jurnal Gamma

Show Abstract | Download Original | Original Source | Check in Google Scholar

Serangkaian penelitian telah dilakukan untuk mendapatkan tanaman anggrek Dendrobium transgenik dengan mengintroduksikan gen gus-A ke protochorm like bodies dengan menggunakan teknik Agrobacterium tumefaciens EHA 105 dan plasmid vektor pCambia 1303. Hasil penelitian menunjukkan bahwa jumlah protochorm like bodies hidup tertinggi pada media antibiotik (VW + 250 mg/l cefotaxime dan VW + 100 mg/l cefotaxime) diperoleh pada perlakuan periode ko-kultivasi selama 1 hari, dimana persentase PLB hidup pada media VW + 100 mg/l cefotaxime mencapai 82%. Peningkakan lama periode ko-kultivasi pada proses transformasi genetik terbukti meningkatkan persentase kematian PLB. Pada penelitian ini, perlakuan periode ko-kultivasi 2 hari dan 3 hari menghasilkan ekspresi gen gus-A tertinggi pada PLB anggrek Dendrobium, dimana persentase PLB positif gen gus-A mencapai 100%. Tetapi, periode ko-kultivasi 3 hari meningkatkan PLB kontaminasi Agrobacterium tumefaciens (46%) dan menurunkan persentase PLB hidup anggrek Dendrobium (30%) pada media VW + 250 mg/l cefotaxime.

PENGEMBANGAN METODE ISOLASI DNA GENOM PADA TANAMAN JARAK PAGAR (Jatropha curcas) Agus Zainudin, Maftuchah
Jurnal Gamma Vol. 2 No. 1 (2006): September
Publisher : Jurnal Gamma

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Molecular markers have been used extensively to study genetic relationships in number of crops. One of the techniques that can be used to obtain DNA finger print is RAPD (random amplified polymorphic DNA) technique, a molecular technique based on PCR technology. The quality of amplification products depend on several factors such as MgCl2 concentration, primers, PCR condition and DNA quality. In this study, an experiment was conducted to find out appropriate DNA isolation technique for three accessions Jatropha curcas (Karangtengah, Lamongan and NTB). Three DNA isolation technique has been tested and two of them were able to produce good quality of genomic DNA. The results therefore indicate that the technique can be used to genomic DNA isolation of Jatropha curcas on molecular analysis.

ANALISIS KERAGAMAN GENETIK TANAMAN JARAK PAGAR LOKAL (JATROPHA CURCAS L.) BERDASARKAN PENANDA MOLEKULER RANDOM AMPLIFIED POLYMORPHIC DNA Maftuchah .
Jurnal Gamma Vol. 5 No. 1 (2009): September
Publisher : Jurnal Gamma

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DNA based RAPD (random amplified polymorphic DNA) markers have been used extensivelyto study genetic relationships in a number of fruit crops. A wide genetic diversity exists in theJatropha curcas in Indonesia. Three accession of Jatropha curcas were selected to assess geneticrelatedness. Total genomic DNA were extracted and subjected to RAPD analysis using 10 primers.Of these, 27 primers ampified Jetropha curcas genomic DNA . Cluster analysis clearly showed twogroups, the first consisting ..... Jatropha curcas and th second groups consisting ..................

INDUKSIVARIASI SOMAKLONAL JARAK PAGAR (JATROPHA CURCAS L.) UNTUK MENDAPATKAN KARAKTERTOLERAN CEKAMAN KEKERINGAN Maftuchah .
Jurnal Gamma Vol. 6 No. 1 (2010): September
Publisher : Jurnal Gamma

Show Abstract | Download Original | Original Source | Check in Google Scholar

Experimental activities is conduct in several stages, that is : somaclonal variation induction incastor oil in vitro culture through the use of PEG and selected plantlet is reproduce in vitro. In vitroselection procedure is done by planting cotyledon explants from castor oil seedlings age 10-14 daysin nutrient medium MS+BAP+IBA. Seed sterilization is done using Tween 20, Benlate, 90% alcohol,50% chloroc and sterile water.After callus is grown, callus will be sub-cultured into nutrient mediumMS+BAP with PEG addition. Selected callus will be transferred into nutrient medium MS + potassiumpantotenat + active coal until it shows root growth.Result of experiment has shown that the higher PEG concentration will suppress thegrowth of castor oil callus. In PEG concentration 15% the mortality rate of castor oil callus (LD) is64,29% and for PEG concentration 20% the mortality rate of castor oil callus is 92,86%. For thenext phase is in vitro selection process through treatment of 15% PEG toward all seven accessions.Result of this experiment has revealed that average mortality rate of callus in 15% PEG treatment is87,64%. HS-49 has shown the lowest percentage rate of response (79%) in 15% PEG treatment,while the highest percentage rate of response occurs for SP-8 (93%). Callus that succeeding growthfrom PEG selection medium will be reproduce in regeneration medium until bud is formed. Buddingconstruct percentage is range between 28,57% (accession HS-49 and SP-38) until 37,03% (accessionSM-35). Average budding construct percentage from selected callus in regeneration medium is31,45%. For the next stage of experiment (second year), those bud will be sub-cultured into rootingmedium until forming root and become complete plantlet ready to acclimatize.

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