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All Journal Jurnal Kesehatan Masyarakat Indonesia Jurnal Kedokteran Diponegoro Althea Medical Journal Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Journal of Biomedicine and Translational Research Jurnal Kesehatan Andalas Biomedical Journal of Indonesia Jurnal Kedokteran dan Kesehatan Jurnal Medika Malahayati Medical and Health Science Journal JURNAL ANALIS LABORATORIUM MEDIK Dharma Raflesia : Jurnal Ilmiah Pengembangan dan Penerapan IPTEKS AgriHealth: Journal of Agri-food, Nutrition and Public Health Jurnal Kedokteran Universitas Palangka Raya Jurnal Kesehatan Tambusai Jurnal Kedokteran Raflesia Jurnal PADAMU NEGERI (Pengabdian pada Masyarakat Bidang Eksakta) JUMANTIK (Jurnal Ilmiah Penelitian Kesehatan) Jurnal Pengabdian Masyarakat As-salam Dikdimas : Jurnal Pengabdian Kepada Masyarakat Proceeding of Bengkulu International Conference on Health Proceeding International Conference Of Innovation Science, Technology, Education, Children And Health Proceeding International Conference Of Innovation Science, Technology, Education, Children And Health
Debie Rizqoh
Departemen Mikrobiologi Dan Imunologi, Fakultas Kedokteran Dan Ilmu Kesehatan, Universitas Bengkulu
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Journal : Biomedical Journal of Indonesia

 1 
Comparison of Widal Test Using Serum and Plasma Samples Rizqoh, Debie; Roina Sitanggang
Biomedical Journal of Indonesia Vol. 7 No. 2 (2021): Biomedical Journal of Indonesia
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v7i2.291

Abstract

The widal test is a test for diagnostic of thyfoid fever serology. Widal test aims to detect the presence of antibodies against Salmonella antigen, by measuring the degree of agglutination of antibody titer to antigen O and H in sample. In this research Widal test was performed using serumand plasma samples. This study aims to determine whether there is a difference in the value of titer on the Widal test between serum samples and plasma EDTA samples. This type of research is an experimental study. Samples were taken from patient suspected of thyfoid fever of 10 sample in the Laboratory of SM Raja Amplas in 2018. The result of comparative titer Widal test using serum and plasma samples obtained results 30% of the 10 samples examined there are differences in the value of Widal titer but does not affect the final diagnosis of typhoid fever. The value of the Widal test titer using plasma is lower than the value of Widal Titer with the serum samples. The use of plasma samples to determine the Widal titer is particularity susceptible to mingling with the erythrocytes due to the presence of anticoagulant particles but does not affect the result of the Widal test. EDTA anticoagulants do not damage the antibodies contained in the serum, this can be seen in the results of the study, there were no differences in each sample examined, so the difference in results obtained due to other factors, namely the error of researchers in the Widal test. The Widal test using serum samples can gives results according to the actual circumstances because serum is not contaminated by EDTA anticoagulants.
Comparison of Widal Test Using Serum and Plasma Samples Debie Rizqoh; Roina Sitanggang
Biomedical Journal of Indonesia Vol. 7 No. 2 (2021): Vol 7, No 2, 2021
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v7i2.291

Abstract

The widal test is a test for diagnostic of thyfoid fever serology. Widal test aims to detect the presence of antibodies against Salmonella antigen, by measuring the degree of agglutination of antibody titer to antigen O and H in sample. In this research Widal test was performed using serumand plasma samples. This study aims to determine whether there is a difference in the value of titer on the Widal test between serum samples and plasma EDTA samples. This type of research is an experimental study. Samples were taken from patient suspected of thyfoid fever of 10 sample in the Laboratory of SM Raja Amplas in 2018. The result of comparative titer Widal test using serum and plasma samples obtained results 30% of the 10 samples examined there are differences in the value of Widal titer but does not affect the final diagnosis of typhoid fever. The value of the Widal test titer using plasma is lower than the value of Widal Titer with the serum samples. The use of plasma samples to determine the Widal titer is particularity susceptible to mingling with the erythrocytes due to the presence of anticoagulant particles but does not affect the result of the Widal test. EDTA anticoagulants do not damage the antibodies contained in the serum, this can be seen in the results of the study, there were no differences in each sample examined, so the difference in results obtained due to other factors, namely the error of researchers in the Widal test. The Widal test using serum samples can gives results according to the actual circumstances because serum is not contaminated by EDTA anticoagulants.
Co-Authors Ade Tiur Rumondang Ade Tiur Rumondang Adiansyah Ahmad Fandi Alvi Jalilul Hakim Ambarsarie, Riry Amelia, Aisyah Annelin Kurniati Annelin Kurniati Arifani, Novita Ashan, Hesty Rhauda Athalia , Nabila Wine Atik Prihatiningrum, Atik Atiqah, Rissya Febiona Azella Chika Fauzia Besly Sinuhaji Besly Sinuhaji Besly Sinuhaji Dendrinson Purba Dendrinson Purba Dessy Triana Dessy Triana Dessy Triana Diah Ayu Aguspa Dita Dian Handayani Djatmiko, Ellen Maidia Ellen Maidia Djatmiko Ellen Maidia Djatmiko Elvira Yunita Elvira Yunita Endang, Jusup Enny Nugrahaeni Enny Nugrahaeni Enny Nugraheni Enny Nugraheni Enny Nugraheni Enny, Nugraheni Ety Febrianti Ety Febrianti Fadlika, Rizki Fahira, Dita Fandi, Ahmad Fauzia, Azella Chika Febrianti, Ety Febrina, Olivia Putri Grace Junita Bate'e Gunasari, Lala Foresta Valentine Hakim, Alvi Jalilul Hamka Ismuda Hestina Hestina Iqbal, Maulana Jusup Endang Kumala, Wulan Okta Kurniati, Annelin Laudy, Nabila Putri Mardhatillah Sariyanti Mardhatillah Sariyanti Mardhatillah Sariyanti Mardhatillah Sariyanti Meidiyanti, Prima Mulya Sundari Mulya Sundari Mulya Sundari Mulya Sundari Mulya Sundari Mulya Sundari Nabilah, Putri Syifa Nikki Aldi Massardi Noor Diah Erlinawati Novita Arifani Nugraheni, Enny Nur Fitri Ocktaviani, Risti Dwi Oktavia Salsadillah Putri Oktavia Silalahi Oktoviani Oktoviani, Oktoviani Pratiwi, Ulfa Hanum Putri Maulida Rachmawati, Indah Dwi Ramadhani, Abdah Zhafirah Rizkianti Anggraini Roina Sitanggang Roina Sitanggang Sariyanti, Mardhatillah Sipriyadi Suryani, Utari Hartati Umar, Liya Agustin Utari Hartati Suryani Utari Hartati Suryani Wulan Okta Kumala