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Journal : BERITA BIOLOGI

VARIASI INTERSPESIFIK JULANG (AVES: BUCEROTIDAE) INDONESIA BERDASARKAN GEN CYTOCHROME-B DNA MITOKONDRIA Jarulis, Jarulis; Solihin, Dedy Duryadi; Mardiastuti, Ani; Prasetyo, Lilik Budi
BERITA BIOLOGI Vol 18, No 1 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i1.3714

Abstract

Genetic information of Indonesian hornbills (Julang) is still limited. We sequenced the cytochrome b gene of three hornbill species (Rhyticeros undulatus, R. plicatus, and Aceros cassidix) to explore their genetic variation, distance, and phylogenetic. Nine blood samples for Cyt b from three hornbill species were isolated with Dneasy® Blood and Tissue Kit base on Spin-Column Protocol, Qiagen. SNP, genetic distance, and phylogeny were calculated by MEGA 6.0 software. This study was found variations in the Cyt b gene sequence (849 bp) between three species Indonesian Julang. Rhyticeros undulatus had the most SNP compared to R. plicatus and Aceros cassidix. Substitution of synonyms is more common than substitution of synonyms and transition substitution frequency tends to be much higher than transversion substitution. The three species of Indonesian Julang were markedly separate with an average genetic distance of 5.25%. and separated with the outgroup species at a genetic distance of 9.43%. Cytochrome b sequence genes from these three hornbill species are novel for identifying and classifying hornbills, and could be applied to prevent illegal poaching and conservation management in Indonesia.    
VARIASI INTERSPESIFIK JULANG (AVES: BUCEROTIDAE) INDONESIA BERDASARKAN GEN CYTOCHROME-B DNA MITOKONDRIA Jarulis Jarulis; Dedy Duryadi Solihin; Ani Mardiastuti; Lilik Budi Prasetyo
BERITA BIOLOGI Vol 18, No 1 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i1.3714

Abstract

Genetic information of Indonesian hornbills (Julang) is still limited. We sequenced the cytochrome b gene of three hornbill species (Rhyticeros undulatus, R. plicatus, and Aceros cassidix) to explore their genetic variation, distance, and phylogenetic. Nine blood samples for Cyt b from three hornbill species were isolated with Dneasy® Blood and Tissue Kit base on Spin-Column Protocol, Qiagen. SNP, genetic distance, and phylogeny were calculated by MEGA 6.0 software. This study was found variations in the Cyt b gene sequence (849 bp) between three species Indonesian Julang. Rhyticeros undulatus had the most SNP compared to R. plicatus and Aceros cassidix. Substitution of synonyms is more common than substitution of synonyms and transition substitution frequency tends to be much higher than transversion substitution. The three species of Indonesian Julang were markedly separate with an average genetic distance of 5.25%. and separated with the outgroup species at a genetic distance of 9.43%. Cytochrome b sequence genes from these three hornbill species are novel for identifying and classifying hornbills, and could be applied to prevent illegal poaching and conservation management in Indonesia.    
Co-Authors ABDUL HARIS MUSTARI Achmad Ariefiandy Achmad, Fariz Aeng Saputra Agnes Ferisa Agus P. Kartono Agus P. Kartono Agus P. Kartono Agus P. Kartono Agus Subagyo Agus, Syamsul B. Alim Setiawan Anas Salsabila Andayani, Noviar Andrew Powling ANDREW POWLING ANDREW POWLING, ANDREW Aprilianti, Risma Aronika Kaban aronika kaban Aronika Kaban Asep Saefullah AURORA PHILLIPS AURORA PHILLIPS AURORA PHILLIPS, AURORA Bangkit Maulana Burhanuddin Masy'ud Burhanuddin Masy'ud Burhanuddin Masy’ud Chandra, Vinoba Chelvin Cahya Kirnanda DEDI SOEDHARMA Dedi Soedharma Dewi Malia Prawiradilaga, Dewi Malia Dhian Eko Prastiwi Dhian Eko Prastiwi, Dhian Eko Djatmiko, Wibowo Agung Elisabet RRB Hutabarat Entang Iskandar Entang Iskandar Entang Iskandar Entang Iskandar Entang Iskandar Entang Iskandar Erny Jumilawaty Fachruddin Majeri Mangunjaya Fadila, Muhammad Imam Fathani, Muhammad Hamas Febriany Iskandar Fransisca Noni Tirtaningtyas Gugah Praharawati Hadi S Alikodra Hafiyyan Sastranegara Hani Sabrina Harnios Arief Harnum Nurazizah Hefni Effendi Hendra Gunawan Hendra Gunawan Hendra Gunawan Hendry Fauzy, Noorman Herawati, Maria Edna Hermawan, Rachmad Heru Setijanto Hiroshi Kobayashi Ida Ayu Ari Janiawati Insan Kurnia Ismi Shanti Qomariah Janra, M. Nazri Jansen Manansang Jarulis Jarulis Jarulis Jarulis Jarulis Jarulis JARWADI BUDI HERNOWO Julius Paolo Siregar Kanthi Hardina Lastri Dwi Saputri Lilik B. Prasetyo Lilik B. Prasetyo Lilik B. Prasetyo Lilik B. Prasetyo Lilik B. Prasetyo Lilik Budi Prasetyo LILIK BUDIPRASETYO Lina Kristina Dewi M. Bismark M. Nazri Janra Mangunjaya, Fachruddin Majeri Mariana Silvana Moy Masy'ud, Burhanuddin Masy’ud, Burhanuddin Maulida, Risqinanti Mennofatria Boer Mia Clarissa Dewi Mirza D. Kusrini Mirza D. Kusrini, Mirza D. Mirza Dikari Kusrini Mirza Kusrini Moh. Ihsan Mohammad Ali Ridha Moy, Mariana Silvana Muhammad Bismark Muhammad Faesal Rahman Hakim Muhammad, Gema Ikrar Munawir, Ahmad Nancy Karraker NUR ANNIS HIDAYATI Nur Annis Hidayati Nurul Khakhim Nurul Khakhim Nyoto Santoso Pradana, Dimas Haryo Praharawati, Gugah Pramunandya, Raka Aditya Purnomo, Harri Purnomo, Harri Randall C. Kyes REBECCA WHEELER REBECCA WHEELER REBECCA WHEELER, REBECCA Reviany Widjakusuma Richard A. Noske Riko Irwanto Rondang Sumurung Edonita Siregar ROSIE PRITCHETT ROSIE PRITCHETT ROSIE PRITCHETT, ROSIE Rumblat, Walid Rushayati, Siti B. Satyawan Sunito Satyawan Sunito Sih Kahono Sih Kahono SIMON T. SEGAR SIMON T. SEGAR SIMON T. SEGAR, SIMON T. Soehartono, Tonny R. Sri Supraptini Mansjoer Susanti, Neneng Susanti, Neneng Susilo, Setyo B. Sutopo Sutopo Suyanti . Tamnge, Fadila TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tiurmaida A.C Gultom Tri Atmoko Tri Atmoko Tri Atmoko Tri Atmoko Tri Atmoko Tri Atmoko Tutut Sunarminto Umar Fhadli Kennedi Vallen Sakti Maulana Vincentius P Siregar Warmetan, Hermanus Widjakusuma, Reviany Wilson Novarino Yasman, Y Yeni A Mulyani Yeni A. Mulyani Yeni A. Mulyani Yeni A. Mulyani Yeni Aryati Mulyani Yeni Mulyani Yohanna .