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All Journal Sainteks Pembangunan Pedesaan Khazanah Pendidikan Techno (Jurnal Fakultas Teknik, Universitas Muhammadiyah Purwokerto) Proceeding Seminar LPPM UMP Tahun 2014 Sain Akuatik Jurnal SOLMA JRST (Jurnal Riset Sains dan Teknologi) Jurnal Perikanan Universitas Gadjah Mada Aquasains : Jurnal Ilmu Perikanan dan Sumberdaya Perairan KACANEGARA Jurnal Pengabdian pada Masyarakat PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Jurnal Pengabdian UNDIKMA Bioscientist : Jurnal Ilmiah Biologi Altifani Journal: International Journal of Community Engagement Proceeding Biology Education Conference Proceedings Series on Social Sciences & Humanities Proceedings Series on Physical & Formal Sciences Dulang: Jurnal Pengabdian Kepada Masyarakat Techno Jurnal Manusia dan Lingkungan Engagement: Jurnal Pengabdian Kepada Masyarakat
Dini Siswani Mulia
Universitas Muhammadiyah Purwokerto
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : Proceeding Biology Education Conference

 1 
PENGHAMBATAN EKSTRAK METANOL DAUN DAN BATANG TUMBUHAN MANGROVERhizophoramucronataTERHADAP PERTUMBUHAN BEBERAPA STRAIN BAKTERI Aeromonas hydrophila Heri Maryanto; Ana Miftahul Janah; Dini Siswani Mulia
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 15, No 1 (2018): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

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Aeromonas hydrophila is one of the pathogenic bacteria that attack freshwater fish. These bacteria are also pathogenic in brackish water fish and sea and other aquatic animals. Treatment of this bacterial attack should be pursued by using natural materials that are more effective and environmentally friendly, one of which is Rhizophora mucronata. This study aims to determine the inhibition of methanol extract of leaves and stems of R. mucronata mangrove plant on the growth of several strains of A. hydrophila bacteria. The experiment was conducted using experimental method with Completely Randomized Design (RAL) 3 factor factorial, ie A factor (type of bacterial strain): A1 (GPl-04), A2 (GL-01), A3 (GL-02), A4 (GJ- 01), A5 (GK-01), and A6 (GB-01); B (plant part): B1 (stem) and B2 (leaf); C (concentration of methanol extract R. mucronata): C1 (0% / control), C2 (10%), C3 (20%), and C4 (30%), with 3 replications. The parameters observed were the results of phytochemical screening test by Thin Layer Chromatography (TLC) and bacterial growth inhibition (clear zone). Data of clear zone were analyzed using Analysis of Variance (Anova) and Duncan Multiple Range Test (DMRT) with 5% test level, while the result of phytochemical screening test with TLC was analyzed descriptively qualitative. The results showed that R. mucronata mangrove plants potentially inhibited the growth of A. hydrophila because it contains flavonoids, alkaloids, terpenoids, and tannins. The type of bacterial strain and concentration of R. mucronata methanol extract had significant effect (P0 <0,05) on the inhibitory zone diameter but the plant part and interaction of the three factors, ie strains of bacteria, plant part, and concentration of R. mucronata methanol extract were not significantly different P> 0.05). Plant parts of both stems and leaves have the same potential as a natural bactericidal material. Bacteria A. hydrophila GL-01 strain was most sensitive, whereas GK-01 strain was most resistant to R. mucronata methanol extract. 10% concentration is an effective and efficient concentration in inhibiting the growth of A.hydrophila bacteria.
Uji Lapang Pakan Bervaksin Aeromonas hydrophila pada Lele Dumbo di Daerah Banyumas Dini Siswani Mulia; Irma Tri Susanti; Heri Maryanto; Cahyono Purbomartono
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 12, No 1 (2015): Prosiding Seminar Nasional XII Biologi
Publisher : Universitas Sebelas Maret

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Abstract

The main problem in the cultivation of African catfish is a disease MAS (Motile Aeromonas Septicemia) Aeromonas hydrophila bacteria attack. In addition, other problems that also resulted in the successful cultivation of this fish is the high price of fish feed and the cost of production for the procurement of the feed. Needs to be studied the use of feed raw materials that utilize industrial waste as well as the use of vaccines in controlling disease MAS. This study aims to apply feed made from 3 waste, namely chicken feathers, pulp, and trash fish, then add the vaccine (vaccine feed) and tested in the field. Field test conducted in the village of Bangsa, District of Kebasen, Banyumas. This study uses a completely randomized design (CRD) with 3 treatments and 5 replications. The treatment consisted of P1 = vaccine feed 10 days; P2 = vaccine feed 15 days, and P3 = no vaccine feed (controls). Feed was given as much as 5% of the weight of fish / day. Research using plastic tarp length x width x height = 80 x 60 x 60 cm. African catfish which used a length of 10-13 cm with a weight of 7.3 to 21.1 g. Fish reared for 8 weeks. Parameters measured were immune response such as antibody titer, weight gain and length, specific growth rate (SGR), as well as survival. Parameters measured were supporting water quality parameters include water temperature, pH, and dissolved oxygen. Data were analyzed using Analysis of Variance (ANOVA) and Duncan Multiple Range Test (DMRT test) with a test level of 5%. The results showed that the vaccine could increase the production of antibody titers (P <0.05) compared to controls. The use of artificial feed of chicken feathers, pulp, and trash fish are added the vaccine (vaccine feed) can be applied in the field.Keywords: Aeromonas hydrophila, Banyumas, field test, vaccine feed
FERMENTASI TEPUNG BULU AYAM DENGAN Bacillus licheniformis B2560 UNTUK MENINGKATKAN KUALITAS BAHAN BAKU PAKANIKAN Dini Siswani Mulia; Yesi Nartanti; Heri Maryanto; Cahyono Purbomartono
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 11, No 1 (2014): Prosiding Seminar Nasional XI Biologi
Publisher : Universitas Sebelas Maret

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Abstract - The purpose of this study is to examine fermentation of chicken feather meal with Bacillus licheniformis B2560 to improve the quality of fish feed ingredients. The study was conducted using an experimental method to completely randomized design (CRD) 4 treatments, 3 replications, ie P0 = non- fermented ; P1 = 5 ml inoculum fermentation with B. licheniformis B2560 ; P2 = 10 ml inoculum fermentation with B. licheniformis B2560 ; and P3 = 15 ml inoculum fermentation with B. licheniformis B2560, respectively for chicken feather meal as much as 2 g .Parameters measured were the levels of protein and organoleptic parameters that support, in the form of the physical properties of chicken feather meal, include color, texture, and smell. Data were analyzed using a protein content analysis of variance (ANOVA) with a confidence level of 95 %, followed by Duncans Multiple Range Test Test (DMRT) with a confidence level of 95 % , while for the data results of the organoleptic qualitatively analyzed descriptively.The results showed that the P0 treatment was significantly different (P < 0.05) with P1, P2, and P3, and between treatments P1, P2, and P3 respectively were significantly different (P < 0.05). The treatment gives the best results is the treatment that P1 has the highest protein content of 84.08 % with a change in the physical properties of white -yellow (color), soft (texture), and less typical sting (smell). Keywords :Bacillus licheniformis B2560, fermentation, the quality of raw materials, chicken feather meal, Fish feed
Co-Authors . Suwarsito Akhmad Darmawan Aman Suyadi Ana Miftahul Janah Ani Khusniah Ardhana Wikaneswari Arief Husin Arif Husin Arif Husin Arif Mahdiana, Arif Berlian Bun’ya Rista Cahyono Purbamartono Cahyono Purbomartono Cahyono Purbomartono Cintya Windarti Danang Priyambodo Didik Trianto Nugroho, Didik Trianto Dwi Ma’rifatun Khasanah Eddy Sumardi, Eddy Eka Yulyanti Eka Yulyanti, Eka Fatih Wulandari Gayuh Prasetyo Budi Hamzah Syarifuddin, Hamzah Haryanto Haryanto Heri Maryanto Heri Maryanto Heri Maryanto Heri Maryanto Heri Maryanto Heri Maryanto Heri Maryanto Heri Maryanto Heri Maryanto, Cahyono Purbomartono, Heri Maryanto, Hery Maryanto, Hery Hindayati Mustafidah Ikhsan Mujahid Irma Tri Susanti Jejentri, Jejentri Juanita Juanita Juli R. Wuliandari, Juli R. Juli Rochmijati Wuliandari Kartika Silfiana, Kartika Miftakhul Mudah, Miftakhul Mochammad Imron Awalludin Muryanto, Muhammad Nadifah, Itqon Ngaynun Nadiya Nur Apreli Noerhaliza Rachman Asriana Dwi Prihandoko, Arief Rachmansyah Rachmansyah Rakhmawati Utami, Rakhmawati Ratna Kartika Wati Restu Frida Utami Rini Emawati Risna Tri Yuliningsih Rofiqoh Rofiqoh Saputra, Eqwar Shahiffa Nur Pranannisa Siti Muntasiroh Sri Wahyuni Sugeng Priyadi Susanto Susanto Susylowati, Dewi Suwarno Suwarno Syamsiar, Syamsiar Syiva Vauziyyah Triyanto Triyanto Ulfatun Rizkiyah Wakhudin, Wakhudin Widjarnako, Beny Widya Apriyanti, Widya Wildan Ahid Mujamal Yanuar Verianto, Yanuar Yasinta, Syaukaty Yesi Nartanti Yusuf Aditya