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Journal : Bioeksperimen

Isolation, Characterization, and Identification of Biosurfactant-Production Bacteria from Used Cooking Oil Waste Fisky Aditya Pratama; Pramesti Dewi; Ibnul Mubarok; Dewi Mustikaningtyas
Bioeksperimen: Jurnal Penelitian Biologi Vol. 11 No. 2 (2025): 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i2.11115

Abstract

Waste cooking oil constitutes a significant environmental concern in Indonesia. It has the potential to be utilized as a substrate for biosurfactant-producing bacteria. Biosurfactants are amphiphilic compounds produced by microorganisms, containing both hydrophilic and hydrophobic components that enable them to dissolve in both water and fats, as well as to reduce surface tension. This study aims to isolate, characterize, and identify biosurfactant-producing bacteria from waste cooking oil. Isolation proccess was conducted using Mineral Salt Medium enriched with cooking oil as an inducer and Carboxy Methyl Cellulose as a carbon source and emulsifier. Two isolates were successfully obtained and purified. Their biosurfactant-producing ability was evaluated through drop collapse test and oil spreading test. One isolate demonstrated superior performance, showing positive activity in both tests, including a 0.7 cm oil spreading zone. Identification involved macroscopic observation, Gram staining, endospore staining, and a catalase test. The isolate exhibited characteristics of a Gram-positive, rod-shaped, spore-forming, catalase-positive bacterium with small, circular, milky-white colonies and a smooth surface. This identification is non-molecular and based on a phenotypic approach according to Bergey’s Manual for preliminary bacterial classification. Based on these features, the isolate is presumed to belong to the genus Bacillus, known for its robust biosurfactant production. The findings suggest that waste cooking oil can be a promising source of indigenous biosurfactant-producing bacteria, contributing to both microbial conservation and potential development of eco-friendly biotechnological products.
Co-Authors Achmadi, Taofan Ali Afiddah, Tawallani Andin Irsadi Andiniyati, Fitri Anggyi Trisnawan Putra Anisa Dewi Sri Puspitasari Annis Aulia Hastuti, Annis Aulia Apriansyah Wibowo Aprilia Indra Kartika, Aprilia Indra Ari Yuniastuti Arif Widiyatmoko, Arif Arvidhea Safira Gunawan Astrid Farmayati Atunnisa, Rifa' Aulia Nur Lita, Aulia Nur Azkiyah, Nimas Catur B., F. Putut Martin H. Bambang Priyono Budi Astuti Burhan, Ilham Damar Galih Darmawan, Melissa Salma Dewi, Novi Ratna Didik Ariwibowo Dinia Rizqi Dwijayanti Elissa, Intan Ayu Ely Rudyatmi, Ely Endah Peniati Fahmi Arifan Faj'ria, Nur Fajriyanti, Lisa Amelia Fatimah Azzahra Febriyanto, Hendra Fidia Fibriana Fisky Aditya Pratama Fitri Arum Sasi, Fitri Arum Fitriyah, Nanda Delva Grahadi, Rahmat Gunawan, Arvidhea Safira Hadikawuryan, Danang Subarkah Hafil Kusuma, Kavana Haris, Amnan Hasyim Abdurrasyid Heriyanti, Andhina Putri Hery Sutarto Hisan, Aisyah Khoirotun Hisan, Aisyah Khoirotun Ibnul Mubarok Ibnul Mubarok Ika Christina, Yuyun Indah Hartati Indriani, Betty Irawati Irawati Isnaeni Karunia Annisa Kaisar Akhmad Khan Kartika Widyaningrum Kholil, Putri Alifa Krispinus Kedati Pukan Kusuma, Kavana Hafil Listiaji, Prasetyo Maftuhah, Anis Maharani, Ria Ika Marlita Marlita, Marlita Martien Herna Susanti Milah, Nihayatul Moch Sasmito Djati Mohamad Endy Yulianto Muhammad Abdullah Na:am, Muh Fakhrihun Nafisah, Wirdatun Nashi Widodo Natalia, Lidya Ayu Nicky, Dhea Nikmatul Hidayah Noor Aini Habibah Nugrahaningsih Nugrahaningsih Nugrahaningsih WH, Nugrahaningsih Nugraini, Anisa Ratna Nur Rahayu Utami Nurul Fadhilah Nurussalma, Syafina Parmin - Pramesti Dewi Priyantini Widiyaningrum Purwadi, Cintiya Egi R Susanti R. Adi Susanti, R. Adi R. Susanti, R. Susanti Rahmawati, Maya Putri Rakainsa, Senda Kartika Ramadani, Mutiara Bintang Retno Sri Iswari Ria Ika Maharani Ria Ika Maharani Rizki Oktavia, Rizki Rizky Ajie Aprilianto Rizqi Dwijayanti, Dinia Rohmah, Ilmiana Nurur Romadhon, Vhaviriele Abel Salsabilla, Nur Hayati Afrilda Sasabila Mumtaz Sekar Jatiningrum Parnonansia Sekar Jatiningrum Parnonansia Sesami, Laut Padi Sih Sigit Saptono Siti Alimah Siti Harnina Bintari Siti Nur Chasanah, Siti Nur Sofiyani, Rakhma Dita Solichin Solichin Solikin, Ahmad Solikin, Ahmad Sri Sukaesih Sudarmin Sudarmin Sunarsih, Sarly Sutiyani, Iis Sutiyani, Iis Talitha Widiatningrum Trida Ridho Fariz Tuti Widianti Ufi Saraswati, Ufi Ulfa, Berlina Maika Ulung Anggraito , Yustinus Walandipa, Ghani Wara, Adisti Danis Weni Ratna Nurlita Hening, Weni Ratna Nurlita Wicaksono, Maulana Malik Widyananda, Muhammad Hermawan Winanti, Ruri Wiwi Isnaeni Wiyanto - Wiyanto Wiyanto Wulan Christijanti Wulansari Prasetyaningtyas, Wulansari Yustinus Ulung Anggraito Yuyun Ika Christina Zaenafree, Intan