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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Hortikultura Indonesia (JHI) Jurnal Ilmu-Ilmu Pertanian Indonesia AGRIVITA, Journal of Agricultural Science Agrin : Jurnal Penelitian Pertanian TERRA : Journal of Land Restoration AGRITROPICA : Journal of Agricultural Sciences Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Gema Agro Akta Agrosia Dharma Raflesia : Jurnal Ilmiah Pengembangan dan Penerapan IPTEKS Community Development Journal: Jurnal Pengabdian Masyarakat JURNAL SIPISSANGNGI: Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian Pada Masyarakat Jurnal Imiah Pengabdian Pada Masyarakat (JIPM)
Rustikawati
Universitas Bengkulu
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Journal : Gema Agro

 1 
Respon Temu Putih dan Temu Mangga dengan Pemberian BA dan 2,4-D secara In Vitro Muhammad Isra’ Aulia; Rustikawati; Entang Inoriah
GEMA AGRO Vol 25 No 2 (2020)
Publisher : Fakultas Pertanian, Universitas Warmadewa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22225/.25.2.2608.92-102

Abstract

Calus and shoot induction of curcuma sp requires the balance amount of auxin and cytokinine which are suitable for the plant species. This study was aimed at evaluating the response of ‘temu putih’ (Curcuma zedoaria Rosc.) and ‘temu mangga’ (Curcuma mangga Val.) to the supplementation of 5 ppm BA and several levels of 2,4-D on the MS medium of in vitro culture. The study was conducted in the Laboratory of Agronomy, Department of Agriculture, Faculty of Agriculture, the University of Bengkulu, from December 2018 to February 2019. The plant material was explans isolated from the buds of curcuma tubers. The experiment was conducted in a completely randomized design arranged in factorial with 2 factors. The first factor was the kinds of curcuma i.e . C. zedoaria and C.mangga. The second factor was the concentration of 2,4–D i.e. 0 ppm, 1 ppm and 2 ppm. The base culture medium was MS with 5 ppm BA. The experimental unit consisted of three botle each of which containing of 1 explant. The treatment was repeated three times amounting to 54 experimental units. The results indicated that both curcuma had different growth respon to in vitro culture. Addition of 2,4-D to the MS medium containing 5 ppm BA produced cally. Callus induction was formed in in vitro culture of Curcuma zeodiaria suplemented with 1 ppm 2,4-D. Up to 2 ppm of 2,4-D, cally production of Curcuma mangga still increasing. Addition of 5 ppm of BA 5 to MS medium induced shoot formation in Curcuma zeodiaria but not in Curcuma mangga.
Co-Authors A.W. Sandhi S.G. Adeputri, Eriana Agus Purwito Almunawar, Said Agil Alnopri Alnopri Alnopri, Alnopri Amanda, Kiki Rizki Andi Tri Maryono Angraini, Dila ARIANDANA WANTHO Atra Romeida Atra Romeida Bambang Gonggo Murcitro Bertham, Yudhy H Bilal Chairul Ahmad Deo Surya Anjaya Dewi Sukma Dival Satria Gunawan Dwi Wahyuni Ganefianti Dwi Wahyuni Ganefianti, Dwi Wahyuni Dwitama, Niko Edhi Turmudi Edi Susilo Eko Suprijono Ela Hasri Windari, Ela Hasri Windari Emilda Tri Mauli Entang Inoriah Entang Inoriah Fahrurrozi, Fahrurrozi Fernandez, Regi Hasanudin Hellianti Pennita Herison, Catur Herry Gusmara Hindarto, Kanang Setyo Indres, Indres Ineu Sulastrini Inoriyah, Entang Joko Suharjo, Usman Kris Kamil, Maulana Insanul Laksono Trisnantoro Luwih, Bagus Edi Maemunah Maemunah Marulak Simarmata Marulak Simarmata Marulak Simarmata Marwanto Marwanto Meko Gustian Merakati Handajaningsih Merakati Handajaningsih Merakati Handajaningsih Merakati Handayaningsih Merakati Handayaningsih Mimi Sutrawati Muhammad Chozin, Muhammad Muhammad Isra’ Aulia Muhammad Isra’ Aulia Muhammad Ravie Mukhtasar Mukhtasar Mukhtasar, Mukhtasar Nabila, Athya Kania Nadia, Nur Ain NESTI FRONIKA SIANIPAR Nurjanah, Uswatun Pardosi, Santi K. Parwito Parwito Reny Herawati Ria Meita S. H. Sutjahjo Saputra, Pringky Okta Saragih, Rosa Sri Andari Sianipar, Mangara W Sri Winarsih Sri Wulandari Sri Wulandari Wulandari, Sri Wulandari Sudarsono Suharjo, Usman Kris Joko Sukisno Sukisno Sukisno Sukisno, Sukisno Surjono H. Sutjahjo Surjono H. Sutjahjo Surjono H. Sutjahjo Surjono Hadi Sutjahjo Surmaini, Ervi Suryati, Dotti Syafira Dwi Wulandari Syafitri, Alya Novia Syakia Kurin, Aricha Umi Salamah Vera Dwisari Viona Juanda Putri Widodo Widodo Wilmar Maarisit Windari, Ela Hasri Wuri Prameswari Yulian, Yulian