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The Effect of Black Cumin (Nigella sativa) on Histopathology of Liver and Kidney in Albino Rats with Organophosphate Exposure Hendrawan, Viski Fitri; Oktanella, Yudit; Firmawati, Aulia; Agustina, Galuh Chandra
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.35-42

Abstract

This study aimed to determine the effect of black cumin extract (Nigella sativa) on the histopathological of the liver and kidneys in albino rats exposed to organophosphates. This study used 20 male mice divided into five treatment groups, i.e. (K-) without organophosphate and black cumin, (K+) induced by organophosphate 40 mg/kg BW, treatment group induced by organophosphate 40 mg/kg BW and black cumin extract as therapy (P1 ) 200 mg/kg BW, (P2) 400 mg/kg BW, and (P3) 600 mg/kg BW. Organophosphate administration for eight weeks and black seed extract therapy was given for three weeks then the mice were euthanized, the liver and kidney organs were taken for histopathological observation using Haematoxylin-Eosin (HE). Data were analyzed using OneWay ANOVA test followed by Tukey test. The results showed that there were significant differences between the treatment groups for liver and kidney damage (p<0,05). Hepatic degeneration and necrosis in the P2 and P3 groups differed from K+ with an average of 2,24±0,9, in the kidneys with an average of 2,52±0,8. It can be concluded that black cumin extract has a significant effect on improving the liver and kidneys in groups P3 (600 mg/kgBW).
Foot and Mouth Disease Impact on Milk Productivity and Quality in KUD Kertajaya, Kediri, Indonesia Oktanella, Yudit; Cahyani, Ayunda Ageng; Hendrawan, Viski Fitri; Nugroho, Widi; Agustina, Galuh Chandra
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.244-249

Abstract

One of the impacts of foot and mouth disease (FMD) on the dairy farming sector is decreasing the quantity and quality of milk production. This study aimed to determine the impact of FMD by observing the productivity and quality of fresh milk during FMD outbreaks. Sampling was performed to obtain productivity data before and during the FMD outbreak using the cluster sampling method in five areas in KUD Kertajaya, Kediri, Indonesia. The lactoscan test was used to analyze the quality of fresh milk from 50 positive samples. Based on the statistical analysis using the paired parametric T-test, it was reported that there was a significant difference in milk productivity before and during the FMD outbreak (p < 0,05). The results of the Lactoscan test on 50 positive samples showed an average fat content of 4,43%, solid nonfat (SNF) content of 8,54%, specific gravity of 1,028, lactose content of 4,67%, total solids content of 12,97%, added water content of 1,44 and freezing point of -0,54°C. In conclusion, the milk of dairy cows during the FMD outbreak often displayed a quality value of milk that met SNI requirements, despite the fact that there was a major decline in productivity.
Efficacy of Black Cumin (Nigella sativa) Ameliorate Organophosphate Toxicity in Pregnant Rats Based on Progesterone Levels and Fetal Bone Development Hendrawan, Viski Fitri; Agustina, Galuh Chandra; Aditya, Siska; Oktanella, Yudit; Firmawati, Aulia; Mariyam, Dewi
Jurnal Medik Veteriner Vol. 7 No. 1 (2024): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss1.2024.134-142

Abstract

Black cumin (Nigella sativa), also referred to as black cumin, is a versatile herbal treatment that has recently grown in popularity, it contains antimicrobial, antibacterial, antifungal, anti-inflammatory, analgesic, antipyretic, antiparasitic, antihistamine, antihypertensive, antitumor, anticancer, antidiabetic, and anti-asthmatic properties are just a few of the positive and pharmacological effects of active ingredients in black cumin, such as thymoquinone and other compounds. This study aimed to evaluate black cumin activity ameliorates organophosphate toxicity in pregnant rats based on progesterone levels and fetal bone development. In this study, pregnant albino rats were divided into five treatment groups i.e. (K-) placebo; (K+) treated an organophosphate; treated an organophosphate and black cumin extract at a dose of (P1) 200 mg/kg BW, (P2) 400 mg/kg BW, and (P3) 600 mg/kg BW. The progesterone levels serum and fetal bone development were evaluated and calculated using ANOVA followed by Turkey. The results showed that P2 and P3 groups significantly increase in progesterone levels compared to K+ and P1 groups. Fetal bone was also reported increase in the growth of caudal vertebrae. In conclusion, 400 mg/kg BW of black cumin could ameliorated progesterone levels and fetal bone development in pregnant rats with organophosphate toxicity.
Phylogenetic and Genetic Variation Analysis of ITS1 Gene of Trypanosoma lewisi in Wild Rats Using Polymerase Chain Reaction Yesica, Reza; Hardian, Andreas Bandang; Oktanella, Yudit; Kusumarini, Shelly; Prasetyo, Gabriela Jeanne Giacynta
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.335-348

Abstract

Murine Trypanosomiasis is a disease caused by the blood protozoan Trypanosoma lewisi in rats, with the transmission process mediated by the flea species Xenopsylla cheopis and Nosopsyllus fasciatus. Cases of trypanosomiasis have been documented due to Trypanosoma lewisi infecting rats and humans in various countries. Diagnosis of T. lewisi is typically conducted using polymerase chain reaction (PCR), which amplifies target DNA using specific primers. One such target gene for detection is the Internal Transcribed Spacer-1 (ITS1). Subsequent sequencing of PCR products enables analysis of genetic variation employing parameters such as nucleotide composition, genetic distance, and phylogenetic analysis with MEGA software. Test results based on percent identity values indicated a 98.51% homology of blood samples with the Chinese strain of T. lewisi (FJ011094.1), demonstrating genetic variation. Phylogram reconstruction revealed that samples 18, 19, and 37 of T. lewisi exhibit very close intraspecies relationships with T. lewisi from NCBI genebank with genetic distance ranging from 0.007 to 0.01. While the closest interspecies relationship was found with T. cruzi (KT305857.1) with a genetic distance of (d = 0.61).
EDUKASI SISTEM KEKEBALAN TUBUH DAN KEBERSIHAN TANGAN DI SEKOLAH DASAR ISLAM BAITUL MAKMUR MALANG, JAWA TIMUR Qosimah, Dahliatul; Oktanella, Yudit; Kusumarini, Shelly
Prosiding Seminar Nasional Unimus Vol 7 (2024): Transformasi Teknologi Menuju Indonesia Sehat dan Pencapaian Sustainable Development G
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Program pengabdian masyarakat bertujuan untuk meningkatkan pemahaman siswa terkait pentingnya kebersihan tangan dan sistem kekebalan tubuh sebagai langkah pencegahan penyakit. Program ini dilaksanakan melalui metode partisipatif yang melibatkan siswa dan guru dalam setiap tahapannya, termasuk edukasi berbasis ceramah, pemutaran video edukatif, serta demonstrasi langsung mengenaiteknik mencuci tangan yang benar. Evaluasi dilakukan menggunakan pre-test dan post-test untuk menilai peningkatan pengetahuan siswa. Hasil dari program ini menunjukkan bahwa sebagian besar siswa sudah memiliki pemahaman yang cukup baik mengenai kebersihan tangan dan kesehatan, dengan skor pre-testrata-rata 98%. Meskipun peningkatan pengetahuan pasca intervensi tidak terlalu signifikan (dengan ratarata post-test 99,5%), kesadaran siswa tentang pentingnya kebersihan, terutama dalam mencuci tangan, meningkat secara substansial. Beberapa konsep, seperti peran sistem kekebalan tubuh dan dampak mikroorganisme patogen, memerlukan penguatan lebih lanjut. Selain itu, pemberian hand sanitizer sebagai bentuk penghargaan bagi siswa yang aktif berpartisipasi terbukti efektif dalam meningkatkan keterlibatan siswa dalam pembelajaran. Kesimpulannya, program edukasi ini berhasil meningkatkan kesadaran siswa tentang pentingnya kebersihan dan kesehatan, meskipun peningkatan pengetahuan bersifat moderat. Rekomendasi pengembangan program meliputi penekanan lebih besar pada konsepkonsep yang kompleks dan pelibatan orang tua serta guru dalam upaya menjaga keberlanjutan praktik kebersihan di rumah dan sekolah. Kata Kunci :  kebersihan tangan; sistem kekebalan tubuh; siswa sekolah dasar; pengabdian masyarakat
KOMUNIKASI INFORMASI DAN EDUKASI (KIE) PENYAKIT LUMPY SKIN DISEASE (LSD) PADA KELOMPOK TERNAK SAPI PERAH Fidi Nur Aini Eka Puji Dameanti; Viski Fitri Hendrawan; Yudit Oktanella; Sruti Listra Adrenalin; Indah Amalia Amri; Ujiati Kamulyan; Fraya Assifa Mardiyah
RAMBIDEUN : Jurnal Pengabdian Kepada Masyarakat Vol. 7 No. 2 (2024): Rambideun: Jurnal Pengabdian Kepada Masyarakat
Publisher : LPPM Universitas Al Muslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51179/pkm.v7i2.2402

Abstract

Lumpy Skin Disease is a smallpox disease on the skin caused by the Lumpy Skin Disease virus from the genus Capripox, family Poxviridae. The LSD virus is double a stranded deoxyribonucleic acid (DNA virus), has a lipid envelope, and replicates in the cytoplasm. This disease can infect cattle and buffalo, and give an economic impact on farmers. Information about LSD in Indonesia has not been published for a long time and has not been published enough, so the Faculty of Veterinary Medicine, Brawijaya University has taken the role of conveying information about LSD through Communication, Information and Education (KIE) media in order to increase awareness of the entry and spread of LSD in areas experiencing LSD outbreaks. The KIE activity was attended by 17 participants from the "Sukses Bersama" Dairy Cattle Group. The results obtained from KIE activities were an increase in participants' knowledge, known from the results of the pretest and posttest analysis. The highest average score before the presentation of the material, the teenage group respondents answered 10 questions correctly, the adult and elderly groups answered 11 questions correctly. Meanwhile, after the presentation of the material, the highest average of respondents in the teenage, adult and elderly groups answered 12 questions correctly. The participant's age influences the participant's ability to answer questions correctly.
KOMUNIKASI INFORMASI DAN EDUKASI (KIE) PENYAKIT LUMPY SKIN DISEASE (LSD) PADA KELOMPOK TERNAK SAPI PERAH Eka Puji Dameanti, Fidi Nur Aini; Hendrawan, Viski Fitri; Oktanella, Yudit; Adrenalin, Sruti Listra; Amri, Indah Amalia; Kamulyan, Ujiati; Assifa Mardiyah, Fraya
RAMBIDEUN : Jurnal Pengabdian Kepada Masyarakat Vol. 7 No. 2 (2024): Rambideun: Jurnal Pengabdian Kepada Masyarakat
Publisher : LPPM Universitas Al Muslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51179/pkm.v7i2.2402

Abstract

Lumpy Skin Disease is a smallpox disease on the skin caused by the Lumpy Skin Disease virus from the genus Capripox, family Poxviridae. The LSD virus is double a stranded deoxyribonucleic acid (DNA virus), has a lipid envelope, and replicates in the cytoplasm. This disease can infect cattle and buffalo, and give an economic impact on farmers. Information about LSD in Indonesia has not been published for a long time and has not been published enough, so the Faculty of Veterinary Medicine, Brawijaya University has taken the role of conveying information about LSD through Communication, Information and Education (KIE) media in order to increase awareness of the entry and spread of LSD in areas experiencing LSD outbreaks. The KIE activity was attended by 17 participants from the "Sukses Bersama" Dairy Cattle Group. The results obtained from KIE activities were an increase in participants' knowledge, known from the results of the pretest and posttest analysis. The highest average score before the presentation of the material, the teenage group respondents answered 10 questions correctly, the adult and elderly groups answered 11 questions correctly. Meanwhile, after the presentation of the material, the highest average of respondents in the teenage, adult and elderly groups answered 12 questions correctly. The participant's age influences the participant's ability to answer questions correctly.
Bahasa Indonesia Bahasa Indonesia Viski Fitri Hendrawan; Galuh Candra Agustina; Yudit Oktanella; Aulia Firmawati; Dewi Mariyam
Jurnal Penelitian Pendidikan IPA Vol 9 No 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.5250

Abstract

The issue plaguing the dairy cattle industry as a whole is low reproductive efficacy due to nutritional and reproductive disorders. To overcome this, nutrients in feed play a very important function. Feed is an essential component of any livestock operation. The requirements for animal protein depend on physiological status and production level. Ruminants can also reduce protein loss by recycling urea, a normally excreted byproduct of protein metabolism. Insecticides are components of pesticides used to kill insects. Organophosphate insecticides (OPs) are phosphate-containing insecticides. Phosphoric acid used to manufacture organophosphate insecticides. This insecticide is notorious for being the most toxic to mammals. This study seeks to determine the effect of feed-containing organophosphates on FSH and LH hormone concentrations in the blood of dairy cows. Mid-lactation For the purpose of this study, Friesian Holstein dairy cattle were divided into two experimental groups, group 1 (first ration) and group 2 (second ration). ANOVA analysis was used for statistical analysis.