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All Journal Jurnal Sain Veteriner Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Journal of Veterinary and Animal Sciences Buletin Veteriner Udayana
Tjokorda Sari Nindhia
Laboratorium Epidemiologi Dan Biostatistik Veteriner Fakultas Kedokteran Hewan Universitas Udayana Bali Indonesia
Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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PILOT PROYEK PEMBANGKIT LISTRIK TENAGA BIOGAS SKALA RUMAH TANGGA T.G.T. Nindhia; I.W. Surata; T.S. Nindhia; I.D.G.P. Swastika
Buletin Udayana Mengabdi Vol 20 No 1 (2021): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1422.407 KB) | DOI: 10.24843/BUM.2021.v20.i01.p14

Abstract

Desa Buahan, Kecamatan Payangan di Kabupaten Gianyar, Bali merupakan desa Agraris dengan sebagian besar penduduknya bermata pencaharian sebagai petani dan peternak. Peternakan khusunya sapi dan babi banyak terdapat di daerah ini dan sangat potensial untuk dikembangkan sebagai sumber energi cadangan khusunya biogas yang diperoleh dari pengolahan limbah peternakan melalui digester anaerobic. Biogas sudah umum digunakan untuk memasak namun penggunaan biogas lebih lanjut untuk pembangkit listrik tenaga biogas perlu dikembangkan dan disebarluaskan. Hal ini disebabkan ketersediaan energI konvensional semakin langka yang menyebabkan harga listrik terus meningkat. Pengabdian ini bertujuan membangun pilot proyek pembangkit listrik tenaga biogas untuk memperkenalkan dan menyebarluaskan teknologi pembangkit listrik tenaga biogas skala rumah tangga yang terjangkau biayanya dan mudah teknologinya di Desa Buahan, Kecamatan Payangan, Gianyar, Bali. Selanjutnya kegiatan ini berhasil membuat pembangkit listrik tenaga biogas dengan kapasitas 1000 watt dengan berbahan bakar biogas dan sudah terpasang dengan baik dilengkapi dengan rumah genset. Penelitian ini juga menghasilkan Kompor biogas tunggu ganda untuk dapat digunakan memasak dengan menggunakan biogas.
APLIKASI DESULFURIZER METODE ADSORPSI DENGAN KARBON AKTIF D.N.K.P. Negara; T.G.T. Nindhia; I.M. Widiyarta; T.S. Nindhia; I.G.K. Dwijana; I.W.A.R. Dana; I.B.P. Purwadnyana; I.G.N.W. Gunawan
Buletin Udayana Mengabdi Vol 23 No 1 (2024): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/BUM.2024.v23.i01.p01

Abstract

Biogas produced from cow dung at the Tulus Nadi Livestock Farmer Group in Banjar Gambih, Buahan Village, Payangan District, has been used as cooking fuel. However, the high sulfuric acid (H2S) content of biogas can reduce fuel quality and shorten the life of the biogas stove because H2S causes the components of the biogas stove to rust. This service aims to reduce the H2S content of biogas through the adsorption method, which employs activated carbon as an adsorbent. The service includes practice and instruction in desulfurizer installation, maintenance, and activated carbon regeneration. The test results show that the breeder understands and can use the desulfurizer tool effectively. The desulfurizer can reduce the H2S content to 95.9%, allowing the biogas produced to be used optimally for daily cooking purposes. Keywords: Biogas, adsorption, activated carbon, livestock waste
Tracking Antigen Expression of Toxoplasma gondii in Mice by Immuno (Cyto) Histochemistry Ida Ayu Pasti Apsari; Ida Bagus Oka Winaya; Tjokorda Sari Nindhia; Ida Bagus Ngurah Swacita
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p08

Abstract

Mouse has been an animal model that is very sensitive towards the Toxoplasma gondii infection. This research infects 30 mice with free-range chicken heart inoculate and the detection of T. gondiiby immunohistochemistry on the infected mice. The purpose of this study is to track T.gondii antigen expression by immune(cyto)histochemistry in mice. Observation over mice was conducted over 10 weeks. After 10 weeks, the mice are examined for its peritoneal fluid by microscopic and immunohistochemistry examination. Continued by the examination of internal organs to track the expression of T.gondii antigens by immunohistochemistry. The study result showed microscopic examinations on peritoneal fluid did not found any cyst or tachyzoites form of T.gondii. Examination of peritoneal fluid by immunohistochemistry was found to detect expressions of T.gondii antigen. Examinations of the mice’s internal organs detected expressions of T.gondii antigen only in the brain by immunohistochemistry examinations.
Pertumbuhan Alometri Lingkar Tubuh Kerbau Jantan Selama Penggemukan di Desa Kalianget, Seririt, Buleleng, Bali Keliat, Dosmonytha Br; Sampurna, I Putu; Nindhia, Tjokorda Sari
Indonesia Medicus Veterinus Vol 12 (6) 2023
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2023.12.6.776

Abstract

Penelitian ini dilakukan di Desa Kalianget, Seririt, Buleleng, Bali. Penelitian ini bertujuan untuk mengetahui potensi pertumbuhan ukuran lingkar tubuh kerbau yaitu lingkar leher depan, leher belakang, lingkar dada, dan lingkar pinggul kerbau jantan selama penggemukan. Kerbau yang digunakan sebagai objek penelitian adalah kerbau lumpur (Bubalis bubalus) dengan kisaran umur 11-74 bulan. Data diambil dengan teknik sampling jenuh yaitu teknik sampel yang diambil adalah semua kerbau jantan yang digemukkan selama tiga bulan yang memenuhi persyaratan untuk diteliti dari segi kesehatan dan keadaan fisiknya. Pengambilan sampel dilakukan secara bertahap, pada tahap pertama diukur 20 ekor kerbau jantan, selanjutnya setelah satu bulan dilakukan pengukuran tahap kedua, dan selanjutnya tahap ketiga setelah satu bulan tahap kedua dilakukan. Total data yang diperoleh dari ketiga tahap pengukuran sebanyak 60 buah data. Data yang diperoleh akan dianalisis dengan regresi model power dengan persamaan alometri Y=aXb. Hasil analisis menunjukkan terdapat perbedaan koefisien korelasi yang nyata antara lingkar tubuh kerbau selama penggemukan. Pertumbuhan dimensi lingkar tubuh dimulai dari lingkar dada, kemudian diikut lingkar pinggul, lingkar leher depan, dan lingkar leher belakang. Lingkar leher depan dan belakang potensi pertumbuhannya lebih tinggi dari pada lingkar dadar dan lingkar pinggul. Dengan mengetahui potensi pertumbuhan lingkar tubuh ternak, maka peternak bisa mengetahui kapan sebaiknya ternak tersebut dipotong.
HISTOLOGIC FEATURES AND GRANULOCYTE WHITE BLOOD CELL COUNTS OF YOUNG AND ADULT SUMBAWA HORSES REARED UNDER THE LAR SYSTEM Angelica Ramadya Putri Candrianisa; Ni Ketut Suwiti; Tjokorda Sari Nindhia; Ni Luh Eka Setiasih; Anak Agung Sagung Kendran; I Ketut Suatha
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2024 Vol. 16 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i04.p21

Abstract

Leukocytes, are a crucial component of the immune system, protecting the body from infections such as bacteria, viruses, fungi, or parasites. This study aims to determine the histological structure of granulocyte white blood cells—basophils, eosinophils, and neutrophils—in young and adult Sumbawa horses. Blood samples were taken from 10 young and 10 adult Sumbawa horses, and histology preparations were made using eosin and methylene blue staining (MDT IndoReagen®). The histological examination and cell counts were conducted using an Olympus CX33 microscope, and data were analyzed with the T-test. The results showed that the histological structure of granulocyte leukocyte in both young and adult Sumbawa horses consisted of round cells with visible cytoplasm and nucleus. Neutrophils have 3-5 lobes with highly variable nucleus shapes and pale pink cytoplasm. The mean number of neutrophils in young Sumbawa horses was 34.20 ± 5.613%, while in adults it was 54.00 ± 6.324%. Eosinophils have a nucleus with two segmented lobes and very clear purplish-red granules in the cytoplasm. The number of eosinophils in young Sumbawa horses was 10.90 ± 2.726%, higher than in adults at 4.10 ± 1.595%. Basophils have very clear purplish-blue granules, with one nucleus filling almost the entire cytoplasm. The mean number of basophils in young Sumbawa horses was 0.20 ± 0.421%, and in adults, it was 0.10 ± 0.316%. In conclusion, the histological images of granulocyte leukocyte in Sumbawa horses show very clear granules, with the number of neutrophils young horses being lower than in adults and eosinophils being higher in young horses than in adults (P<0.05). However, there was no significant difference in the number of basophils (P>0.05). Further research is needed to compare the histology and agranulocyte white blood cell counts in Sumbawa horses to obtain more comprehensive information.
COMPARISON OF ANTIBODY TITERS IN BROILERS GIVEN GREEN MENIRAN EXTRACT AT DIFFERENT DOSES FOR TWO WEEKS BEFORE NEWCASTLE DISEASE BOOSTER VACCINATION Dewa Kade Agung Hendra Putra Dinata; Ida Bagus Kade Suardana Suardana; I Ketut Suada; Ni Luh Eka Setiasih; I Made Kardena; Tjokorda Sari Nindhia
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p26

Abstract

Newcastle Disease (ND) is a viral disease that affects poultry, including broilers. One of the preventive measures is vaccination; however, its effectiveness remains suboptimal, necessitating an alternative approach such as green meniran (Phyllanthus niruri Linn.) extract to enhance antibody titers. This study aims to determine the differences in antibody titers in broilers administered green meniran extract at different doses for two weeks before the ND booster vaccination. The study involved 30 broilers aged 1 to 32 days. The experimental design followed a Completely Randomized Design with a hierarchical pattern consisting of three treatment groups. Serum samples were collected one day before the ND booster vaccination (17 days old), oneweek post-booster vaccination (25 days old), and two weeks post-booster vaccination (32 days old). The serum samples were tested using the Hemagglutination Inhibition (HI) assay. Data were analyzed using Analysis of Variance (ANOVA) and regression analysis. ANOVA results showed that the treatment group (P2) receiving green meniran extract at a dose of 6 mg/kg BW/day exhibited a significant increase (P<0.05) compared to the treatment group (P1) receiving 4 mg/kg BW/day. Regression analysis indicated that each group experienced an increase in antibody titers up to the fourth week post-booster vaccination. The findings suggest that different sampling times significantly (P<0.05) influenced the antibody titers formed. In conclusion, green meniran extract as a natural immunostimulator can enhance the immune response in broiler chickens. The combination of green meniran extract at doses of 4 mg/kg BW/day and 6 mg/kg BW/day for two weeks with ND booster vaccination proved to be more effective in increasing ND antibody titers in broilers than vaccination alone.
CASE STUDY: COLISEPTICEMIA IN A 1-MONTH-OLD PIGLET IN TARO VILLAGE, GIANYAR, BALI Aryani, Fira Aulia; Widyasanti, Ni Wayan Helpina; Berata, I Ketut; Oka, Ida Bagus Made; Nindhia, Tjokorda Sari
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2026 Vol. 18 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2026.v18.i01.p09

Abstract

Pig farms play an important role in the socio-cultural life of communities, but some of them are intensively managed with poor biosecurity and health management practices, as well as traditional and unhygienic husbandry practices, thereby potentially increasing the risk of health problems in livestock. Colisepticemia is a disease caused by infection with pathogenic Escherichia coli, which primarily affects piglets from the neonatal to post-weaning period. The subject of this case study was a one-month-old Landrace piglet from a smallholder farm in Taro Village, Tegallalang District, Gianyar Regency, Bali. This study aimed to determine the cause of death of the piglets based on epidemiological data, clinical examination, anatomical pathology, histopathology, bacteriology, and parasitology. Epidemiological data showed a total herd population of 455 pigs, with morbidity of 1.76%, mortality of 1.76%, and a case fatality rate (CFR) of 100%. Clinical signs observed in the piglet included anorexia, weakness, vomiting, yellowish-white diarrhea, and inability to stand. The piglet died after showing clinical signs for 16 days. Gross pathological examination revealed congestion in the brain, heart, lungs, liver, kidneys, and intestines, as well as hemorrhage in the trachea, lungs, and intestines. Histopathological examination showed that almost all organs had congestion, hemorrhage, and inflammatory cell infiltration predominantly composed of neutrophils. Bacterial isolation from the lung, heart, liver, and intestines demonstrated growth of Gram-negative Escherichia coli. Parasitological examination using direct smear, sedimentation, and flotation methods yielded negative results for protozoan and helminth infections. Based on these findings, the piglet was diagnosed with colisepticemia.
Co-Authors ANAK AGUNG NGURAH OKA PUJAWAN Anak Agung Sagung Kendran Ananda, Made Krisna Angelica Ramadya Putri Candrianisa Aryani, Fira Aulia Brigita Galilea Adu Cahyani, Ni Luh Risna D.N.K.P. Negara Dewa Kade Agung Hendra Putra Dinata Dwi Arum Permatasari Elfani Sarah Faradina Elizabeth Kezi Damayanti Florensia, Dheadora Geidha Lailia Luzain I Gusti Agung Ayu Suartini I Gusti Komang Dwijana I Gusti Ngurah Kade Mahardika I Gusti Ngurah Sudisma I K. SUATHA I Ketut Berata I Ketut Suada I Ketut Suatha I Made Bagus Arya Permana Ardiana Putra I Made Kardena I Made Widiyarta i Nengah Wandia I Nyoman Dodik Gunawan I Nyoman Suarsana I Nyoman Suartha I Putu Sampurna I Putu Windhu Mahardika I Wayan Batan I Wayan Suardana I Wayan Suardana I Wayan Surata I.B.P. Purwadnyana I.D.G.P. Swastika I.D.N.P. Swastika I.G.N.W. Gunawan I.W.A.R. Dana Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Bagus Bagus Kade Suardana Ida Bagus Kade Suardana Ida Bagus Kade Suardana Ida Bagus Kade Suardana Suardana Ida Bagus Ketut Indra Permana Ida Bagus Made Oka Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya Ihtifazhuddini, Fiqi Manaya Tibyana Iwan Harjono Utama Kadek Karang Agustina Kakang, Dorteany Mayani Keliat, Dosmonytha Br Ketut Ari Andhita Badraresta Arnaya Ketut Ayu Cudemani Putri Korbinianus Feribertus Rinca Kristianto, Kartika Leonardo, Edo Lijung, Derfina Mahindra, Aditya Try Marson, Fransiska Gratia Sonita Messy Saputri Boru Sembiring Negara, Putu Maha Suta Ni Ketut Suwiti Ni Luh Eka Setiasih Ni Luh Eka Setiasih Ni Luh Made Ika Yulita Sari Hadiprata Ni Made Suksmadewi Wisnantari Ni Nyoman Werdi Susari Nicolas Yarisetouw, Nicolas Oktryna Hodesi Sibarani Pero, Fransisco Victoriano Pratama, Rendi Tegar Putra, I Made Wira Diana Sadipun, Elizabeth Liliane Sri Kayati Widyastuti Sunarko, Stefanie Nadya Stellanora Suputra, Gusde Wahyu Krisna Suriana, Ni Komang Valerie Tjokorda Gde Tirta Nindhia Tomáš Ruml Widyasanti, Ni Wayan Helpina Yizhar Eka Zdenek Knejzlík