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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Majalah Obstetri dan Ginekologi Majalah Kesehatan FKUB Medical Laboratory Technology Journal Health Notions Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Indonesian Journal of Medicine JKM (Jurnal Kebidanan Malahayati) MMJ (Mahakam Midwifery Journal) Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia JOURNAL LA MEDIHEALTICO Devotion: Journal of Research and Community Service Asian Journal of Health Research Jurnal Info Kesehatan
Tatit Nurseta
Department Of Obstetric And Gynecology, Faculty Of Medicine, Universitas Brawijaya, Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Journal : Medical Laboratory Technology Journal

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Cowpea (Vigna unguiculata) Extract Reduce Malondialdehyde Levels and Prevent Aortic Endothelial Cell Decline in Ovariectomized Rats Ni Putu Sri Haryati; Elisa Danik Kurniawati; Tanti Tri Lestary; Eviana Norahmawati; I Wayan Arsana Wiyasa; Dwi Yuni Nur Hidayati; Tatit Nurseta
Medical Laboratory Technology Journal Vol. 7 No. 2 (2021): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.221 KB) | DOI: 10.31964/mltj.v0i0.402

Abstract

Estrogen has anti-oxidative and anti-inflammatory properties, but its levels decrease in postmenopausal women who can trigger oxidative stress. One of the most damaging effects of ROS is lipid peroxidation, and the end product is Malondialdehyde (MDA). Similarly, aging endothelium has increased oxidative stress and endothelial cell sensitivity to apoptosis. This study aimed to determine the effect of cowpea extract on serum MDA levels, aortic endothelial cell counts, and brain MDA levels in the ovariectomy model. Cowpea extract can be used as an alternative to prevent and overcome the effects that occur during menopause, such as cardiovascular problems, decreased bone mineral density, and dementia. The study used 15-month-old female Rattus norvegicus, divided into six groups (OVX, SHAM, OVX+estradiol, OVX+Vu 1.25; 2.5; and 5 mg/kg BW/day). Serum and brain MDA levels were examined by ELISA method, while the number of aortic endothelial cells were examined on histopathological preparations with Hematoxylin & Eosin (HE) staining. The mean value of serum and brain MDA levels decreased with an increase in the dose given (p-value 0.016). The mean value of aortic endothelial cells between the dose groups did not significantly differ. However, the mean value showed an increasing trend as the dose of cowpea extract was given. The results of this study indicate that the extract of cowpea has the potential as an antioxidant to reduce serum and brain MDA levels, prevent a decrease in the number of aortic endothelial cells. As prevention, cowpea extract can be used as an antioxidant and consumed since premenopause to minimize problems that occur during postmenopause.
Negative Effect of Cigarette Smoke: Black Garlic Opportunities for Prevention of Ovulation Disorders Asih, Sari Wulan; Nurseta, Tatit; Handono, Kusworini; Yudhanto, Hendy Setyo
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.585

Abstract

Women's issues are primarily related to infertility. This study aimed to demonstrate that ovarian healing in wistar rats exposed to cigarette smoke was impacted by the administration of extracted black garlic. This research uses True Experimental methods in vivo in the laboratory to identify the cause and effect of the variables being tested. This method involves replication, randomization, and control. The design chosen was a post-test-only control group design, where the experimental group received treatment and the control group did not, without random selection. The results of the study showed that there was no effect due to exposure to cigarette smoke with an increase in cortisol and a decrease in the number of secondary follicles in the ovaries of female wistar rats as well as the administration of Black Garlic (Allium sativum) extract. Black garlic extract unaffected cortisol levels and the number of secondary follicles or repair of the ovaries due to exposure to cigarette smoke. Further research is needed in dosing black garlic extract or combining it with other ingredients to provide effective results.
Co-Authors A.A. Ketut Agung Cahyawan W AA Sudharmawan, AA Adys Werestandina, Adys Agustina Tri Endharti Ambar Dwi Retnoningrum Anandasari , Noni Fidya Ayu Anandasari, Noni Fidya Ayu Angelica, Aina Anisak, Siti Asih, Sari Wulan Bambang Rahardjo Cholid Rohman Riskianto Diana Lyrawati Dwi Yuni Nur Hidayati Elisa Danik Kurniawati Endah Wijayanti Eviana Norahmawati Fajar Ari Nugroho Frenty Hadiningsih, Eka Hidayat Sujuti Husnul Khotimah Indahwati, Lilik Indriati Dwi Rahayu jundyah, nazhifa zalfa Juniartha, Putu Karyono Mintaroem Kaspirayanthi, Ni Ketut Devy Kenty Wantri Anita, Kenty Wantri Kridayanti, Novalia Krisna Murti Kusworini Handono Leksono, Setio Lestari, Ni Putu Yunita Sri Mudinillah, Adam Muhammad Irsan Saleh Muhammad Rasjad Indra Muhammad Rivai Mukhamad Nooryanto Natalina, Riny Ni Putu Sri Haryati Noorhamdani Novida Ariani Permata, Dear Ayu Prawiro, Sumarno Reto Putri Ardela, Mayasari Rahajeng Rahajeng Rahmi Utami Ratnawati, Linda Ratnawati, Putu A.R. Sastradinata, Irawan Selvi Marcellia Sitompul, Barlian Sri Winarsih Subandi Subandi SURYANTI Syah Sembung Wasiso Tanti Tri Lestary Tri Yudani MR Wardani, Widya Kusuma Widowati, Ayu Rizky Wijasa, Arsana Wisnu, Gusti Ayu Made Pratishita Wiyasa, I W.A. Wiyasa, I Wayan Arsana Yahya Irwanto Yudhanto, Hendy Setyo Yudistira Yunus