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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Majalah Obstetri dan Ginekologi Majalah Kesehatan FKUB Medical Laboratory Technology Journal Health Notions Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Indonesian Journal of Medicine JKM (Jurnal Kebidanan Malahayati) MMJ (Mahakam Midwifery Journal) Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia JOURNAL LA MEDIHEALTICO Devotion: Journal of Research and Community Service Asian Journal of Health Research Jurnal Info Kesehatan
Tatit Nurseta
Department Of Obstetric And Gynecology, Faculty Of Medicine, Universitas Brawijaya, Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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The Effect of Human Pellucide Zone 3 Monoclonal Antibody on Expression of Bcl-2 and Bax in Follicle Granulosa Cells of Mice Ovary Natalina, Riny; Nurseta, Tatit; Winarsih, Sri
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (393.255 KB) | DOI: 10.11594/jtls.08.02.09

Abstract

Pellucide zone 3 (ZP3) involves in fertilization mechanism. Moreover, antibody of ZP3 can develop to inhibit egg and sperm interaction. This study aims to determine the effect of hZP3 (mab-hZP3) monoclonal antibody on the expression of Bcl-2 and Bax in follicle granulosa cells of the mice ovary. Female mice BALB/c were divided into 12 groups which consisted of control and experimental treatment group. Each group was added with 30% of total mice as error sample (1 mice). Each groups were treated differently: 50 µl adjuvant Al(OH)3 in 50 µl Tris HCl, 20 µg Mab-hZP3, 40 µg Mab- hZP3, and 60 µg Mab-hZP3. Each group was dissected at day 10, 15 and 20. Measurement of Bcl-2 and Bax was performed with immunohistochemistry. Data was analyzed by Two-Way ANOVA. There was no significant effect of Mab-hZP3 administration in various doses on Bcl-2 (p=0.0825), and Bax (p=0.836). There was no significant effect of administration of Mab-hZP3 in time (p=0.807), neither on Bcl-2 expression (p=0.088) and Bax (p=0.227). The lowest Bcl-2 level was found in dose of 60 µg in day 15. There was no significant effect of Mab-hZP3 in various doses and time (p=0.691), neither to Bcl-2 and Bax. Such results obtained due to the specificity of a monoclonal antibody that recognizes specific antigen. Mab-hZP3 is proposed as immunocontraception for women causing no disturbance of folliculogenesis.
Agarose Coated Culture Plate in Tumorsphere Culture of Cervical Cancer Cell Line HeLa: an Alternative to Non Adhesive Culture Plate Juniartha, Putu; Indra, Muhammad Rasjad; Sujuti, Hidayat; Lyrawati, Diana; Nurseta, Tatit
Journal of Tropical Life Science Vol 6, No 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.11

Abstract

Cervical cancer recurs in 90% cases and linked to cancer stem cells that able to self-renew and responsible for recurrence, metastasis, and mortality of cancer. Isolation and identification of cancer stem cells using serum-free medium needs expensive growth factors and consume time. This study try to grow tumor sphere using culture plate coated with 1% agarose as an efficient and economical alternative to non-adhesive culture plate. HeLa cell line was grew in culture plate coated with 1% agarose and non-adhesive culture plate using similar medium and culture condition. Tumor spheres morphology was observed and the colonies were counted in 7 days followed by single cell assay. Tumor spheres then counted for CD133+, CD34+, and Sox2 expression using flowcytometry. Culture plate coated with 1% agarose can be used as an economic and efficient alternative to culture tumor sphere. Using culture plate coated with 1% agarose, the tumor spheres formed in 7 days with similar morphology to non-adhesive culture plate. Tumorsphere had three dimensional – sphere shape that tightly attached, colonized, and overlapped. The tumor sphere colony counts of two plates were statistically have no significant difference (p=0,667). Single cell assay of a tumor sphere shows that it can grow new tumor spheres with similar morphology. The tumor sphere from culture plate coated with 1% agarose express CD133+ and CD34+ as much as 8.78% ± 2.14 and Sox2 as much as 35.30% ± 23.82 whereas tumor sphere from non-adhesive culture plate express CD133+ and CD34+ as much as 62.36% ± 1.06 and Sox2 as much as 98.86% ± 0.56 (p = 0000).
Co-Authors A.A. Ketut Agung Cahyawan W AA Sudharmawan, AA Adys Werestandina, Adys Agustina Tri Endharti Ambar Dwi Retnoningrum Anandasari , Noni Fidya Ayu Anandasari, Noni Fidya Ayu Angelica, Aina Anisak, Siti Asih, Sari Wulan Bambang Rahardjo Cholid Rohman Riskianto Diana Lyrawati Dwi Yuni Nur Hidayati Elisa Danik Kurniawati Endah Wijayanti Eviana Norahmawati Fajar Ari Nugroho Frenty Hadiningsih, Eka Hidayat Sujuti Husnul Khotimah Indahwati, Lilik Indriati Dwi Rahayu jundyah, nazhifa zalfa Juniartha, Putu Karyono Mintaroem Kaspirayanthi, Ni Ketut Devy Kenty Wantri Anita, Kenty Wantri Kridayanti, Novalia Krisna Murti Kusworini Handono Leksono, Setio Lestari, Ni Putu Yunita Sri Mudinillah, Adam Muhammad Irsan Saleh Muhammad Rasjad Indra Muhammad Rivai Mukhamad Nooryanto Natalina, Riny Ni Putu Sri Haryati Noorhamdani Novida Ariani Permata, Dear Ayu Prawiro, Sumarno Reto Putri Ardela, Mayasari Rahajeng Rahajeng Rahmi Utami Ratnawati, Linda Ratnawati, Putu A.R. Sastradinata, Irawan Selvi Marcellia Sitompul, Barlian Sri Winarsih Subandi Subandi SURYANTI Syah Sembung Wasiso Tanti Tri Lestary Tri Yudani MR Wardani, Widya Kusuma Widowati, Ayu Rizky Wijasa, Arsana Wisnu, Gusti Ayu Made Pratishita Wiyasa, I W.A. Wiyasa, I Wayan Arsana Yahya Irwanto Yudhanto, Hendy Setyo Yudistira Yunus