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All Journal Jurnal Farmasi Klinik Indonesia Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Panrita Abdi - Jurnal Pengabdian pada Masyarakat Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Majalah Farmasi dan Farmakologi (Trends in Pharmacy and Pharmaceutical Sciences) Jurnal Respirologi Indonesia Jurnal farmasi UIN Alauddin Makassar Narra J Journal of Experimental and Clinical Pharmacy (JECP) Jurnal Ilmiah Kesehatan (JIKA) Borneo Journal of Pharmacy
Elly Wahyudin
Department Of Pharmacy, Faculty Of Pharmacy, Universitas Hasanuddin, Makassar
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Other

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Journal : Narra J

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A fruit fly-based approach to unraveling enteropathy-causing pharmaceuticals Pratama, Muhammad R.; Wahyudin, Elly; Putri, Tenri ZAD.; Hardiyanti, Widya; Fatiah, Dewita; Chaeratunnisa, Rizkya; Bapulo, Nurdewi N.; Latada, Nadila P.; Mudjahid, Mukarram; Nainu, Firzan
Narra J Vol. 4 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i2.898

Abstract

Enteropathy is a gastrointestinal disorder characterized by inflammation in the small intestine and one of the causes of enteropathy is the side effects of certain drugs, such as non-steroidal anti-inflammatory drugs (NSAIDs). The mechanism of NSAIDs, such as indomethacin, could inhibit prostaglandin synthesis, leading to a decrease in mucus production and small intestine integrity. To test the effects of a drug, it is necessary to undergo preclinical testing using animal models. Commonly used animal models such as mice and rats have several drawbacks including high cost, ethical issues, and long lifespan. Therefore, alternatives such as using invertebrate animals like Drosophila melanogaster as a more economical in vivo platform with genetic similarity to mammals and devoid of ethical concerns are needed. The aim of this study was to evaluate Drosophila melanogaster as an in vivo model organism in testing the side effects of pharmaceuticals that cause enteropathy. In this study, flies aged 3–5 days were starved and then placed into treatment vials comprising untreated control and indomethacin-treated (3.75 mM, 7.5 mM, and 15 mM). Survival analysis was conducted during the treatment period, followed by a Smurf assay test after seven days of treatment. Subsequently, the expression of pro-inflammatory cytokine-related genes (drs and totA), mitochondria stability-related genes (tom40), and endogenous antioxidant-related genes (sod1, sod2, and cat) was performed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Our data indicated that indomethacin did not impact lifespan or cause intestinal damage. However, we observed increased expression of pro-inflammatory cytokine-related genes, including drs, and a twofold increase in totA gene expression. Furthermore, there was a significant upregulation of mitochondrial stability gene tom40, endogenous antioxidant genes sod1 and cat, and a threefold increase in sod2 at 15 mM indomethacin. Although no phenotypical changes in gut integrity were detected, the increased expression of pro-inflammatory cytokine genes suggests the occurrence of inflammation in the indomethacin-treated flies.
Undernutrition-induced stunting-like phenotype in Drosophila melanogaster Putri, Tenri ZAD.; Wahyudin, Elly; Pratama, Muhammad R.; Fatiah, Dewita; Hardiyanti, Widya; Chaeratunnisa, Rizkya; Latada, Nadila P.; Fatmawati, Fatmawati; Mudjahid, Mukarram; Nainu, Firzan
Narra J Vol. 4 No. 3 (2024): December 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i3.999

Abstract

Stunting resulting from undernutrition is a significant global health challenge, particularly in developing countries, yet its underlying mechanisms and consequences remain inadequately understood. This study utilizes Drosophila melanogaster as an in vivo model to investigate the molecular basis of stunting. Due to the conserved nature of signaling pathways between Drosophila and vertebrates, this organism serves as an effective model for studying growth disorders. The aim of this study was to establish a Drosophila model exhibiting a stunting-like phenotype and to elucidate the molecular mechanisms underlying this condition. The stunting phenotype was induced through dietary manipulation, involving a standard nutrient-rich diet (100%) and treatment diets with reduced concentrations of sucrose, glucose, yeast, and cornmeal at 50%, 25%, and 12.5%. Phenotypic assessments included measurements of larval body size, fecundity, survival rates, and locomotor activity, alongside molecular analyses of gene expression related to metabolism, cell proliferation, and survival, using RT-qPCR. Results demonstrated that undernutrition profoundly affected D. melanogaster, causing growth retardation, reduced larval body size, diminished fecundity, and lower survival rates, though locomotor function remained unaffected. Molecular analysis revealed a significant decrease in the expression of the totA gene and notable increases in the expression of dilp5, srl, and indy genes, with no significant changes observed in the expression of the pepck gene. These findings indicate that undernutrition induces a stunting-like phenotype, likely driven by alterations in the expression of genes associated with metabolism, cell proliferation, and survival. Overall, this study establishes D. melanogaster as a valuable in vivo model for studying stunting-like phenotypes resulting from nutritional deficiencies and provides insights into the molecular pathways involved in growth impairment.
KOMPAK: Translation, cross-cultural adaptation, and validation of an instrument for assessing interprofessional collaboration between pharmacists and physicians in Indonesia Amin, Irnawati H.; Massi, Muhammad N.; Wahyudin, Elly; Bahar, Muhammad A.; Hadju, Veni; Niamuzisilawati, Eva; Munawwaroh, Makiyatul; Utami, Ikrimah N.; Ardiansyah, Akhmad; Alkaff, Sylmina D.; Arifin, Bustanul
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.1309

Abstract

Interprofessional collaboration is crucial for addressing the complexity of health problems, requiring contributions from various professions to enhance healthcare quality, improve patient satisfaction, and achieve better clinical outcomes. The aim of this study was to develop and validate the physicians and pharmacists collaborative practice instrument, known as KOMPAK (Kolaborasi Medis Persepsi Apoteker dan Dokter/Medical Collaboration: Perceptions of Pharmacists and Physicians) for use in Indonesia. A cross-sectional study was conducted across the western, central, and eastern rigors of Indonesia, targeting physicians and pharmacists. The study included translation (forward and backward), cross-cultural adaptation (with 30 participants), and validation using confirmatory factor analysis (CFA) among 315 physicians and 315 pharmacists. The present study found no significant changes emerged during the translation and adaptation phases. In the validation phase, the CFA results for the physician instrument indicated a Comparative Fit Index (CFI) of 0.94 (>0.92), Tucker-Lewis Index (TLI) of 0.93 (>0.92), Root Mean Squared Error of Approximation (RMSEA) of 0.055 (<0.07), Standardized Root Mean Residual (SRMR) of 0.07 (<0.08), and Cronbach’s Alpha reliability of 0.88. The pharmacist instrument yielded similar results with a CFI of 0.94 (>0.92), TLI of 0.93 (>0.92), RMSEA of 0.06 (<0.07), SRMR of 0.05 (<0.08), and Cronbach’s Alpha reliability of 0.83. The final instrument consists of 24 items. In conclusion, the KOMPAK instrument demonstrated validity and reliability, supporting its use for measuring interprofessional collaboration between physicians and pharmacists in Indonesia.
Chemical fingerprinting and antioxidant properties of Glochidion philippicum Khairuddin, Khairuddin; Manggau, Marianti A.; Rante, Herlina; Hardiyanti, Widya; Latada, Nadila P.; Umar, Abdul H.; Nur, Syamsu; Wahyudin, Elly; Rahman, Latifah; Yulianty, Risfah; Nainu, Firzan
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.1886

Abstract

Glochidion philippicum has been suggested to exhibit considerable pharmacological potential, yet its chemical composition and bioactivity remain inadequately explored. The aim of this study was to investigate the chemical fingerprint and antioxidant properties of G. philippicum leaf extracts using Fourier-transform infrared spectroscopy (FTIR) with chemometric analyses, and in vitro and in vivo evaluations. Four extraction methods (maceration, reflux, ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE)) were optimized with water, 70% ethanol, ethyl acetate, and n-hexane as solvents. FTIR profiles were analyzed with principal component analysis (PCA), hierarchical cluster analysis, and orthogonal partial least squares discriminant analysis. An in vitro study assessing the free radical scavenging capacity was conducted using the 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric-reducing antioxidant power (FRAP) methods, while in vivo evaluations were conducted using Drosophila melanogaster to measure antioxidant enzyme activity and expression of endogenous antioxidant-related genes. FTIR profiles identified functional groups contributing to antioxidant activity. In vitro assays using ABTS and FRAP methods revealed that extracts obtained with 70% ethanol and water exhibited the highest antioxidant activity, attributed to key functional groups such as C=C (aromatic), O−H (acidic), N=O (nitro), and C−O (ester). In vivo studies showed that ethanol-based MAE extracts (MAEEO) significantly improved the survival of autoinflammatory PGRP-LBΔ mutant larvae exposed to heat-killed Escherichia coli. Real-time quantitative PCR analysis indicated this effect was dependent on endogenous antioxidant gene activation. The study highlights that G. philippicum leaf extracts as a natural source of bioactive compounds with exogenous antioxidant properties, offering potential for therapeutic applications.
Assessing health-related quality of life in schizophrenia patients using EQ-5D-5L index: Insights from patients and caregivers A. Prasetiyo, Nugraha; Wahyudin, Elly; Setiawan, Iman; Sanusi, Mayamariska; Purba, Fredrick D.; Arifin, Bustanul; Alkaff, Sylmina D.
Narra J Vol. 5 No. 2 (2025): August 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i2.1314

Abstract

Schizophrenia is a prevalent mental health disorder often marked by relapses, significantly affecting the health-related quality of life (HRQoL) of both patients and their families. The aim of this study was to compare the EuroQol 5-Dimension 5-level (EQ-5D-5L) responses of schizophrenia patients and their caregivers. Using an observational cross-sectional design, HRQoL was assessed among schizophrenia patients and their family caregivers recruited from a provincial referral hospital. Sociodemographic (age, sex, education, marital status, income) and clinical variables (diagnosis, treatment duration, comorbidities) were analyzed alongside HRQoL using structured interviews, medical record reviews, and the EQ-5D-5L instrument (self-report by patients and proxy-reported by family caregivers). Statistical analyses included chi-square tests for associations, Wilcoxon tests for patient-family caregiver comparisons, and multivariate modeling of HRQoL determinants. A total of 526 participants (263 patients and 263 accompanying family caregivers) were included. Significant differences were observed between patients and family caregivers in two domains: pain/discomfort and anxiety/depression. Also, the agreement between patients' and family caregivers’ reports showed good results. A substantial agreement was observed between patient-reported and family caregiver-assessed HRQoL, as indicated by a Cohen’s Kappa value of 0.8. This result suggests a strong level of consistency between the two assessments, supporting the potential use of family caregivers as reliable proxies for evaluating patient HRQoL when self-reports are unavailable or unreliable. In the self-care domain, mobility, and daily activities, patient and caregiver assessments show high agreement. In conclusion, the closeness between patients and caregivers significantly influences patients' HRQoL, providing critical insights for evaluating treatment effectiveness in schizophrenia cases. While discrepancies exist between patient and caregiver assessments, these interactions are particularly impactful in subjective domains like pain/discomfort and anxiety/depression, but not for other domains that are visible.
Stigma and health-related quality of life (HRQoL) among people with multidrug-resistant tuberculosis (MDR-TB): A cross-sectional study in Indonesia Arifin, Bustanul; Sarwadan, Muhamad G.; Wahyudin, Elly; Sarifah, Latifah M.; Fuady, Ahmad; Purba, Fredrick D.; Alkaff, Sylmina D.; Ardiansyah, Akhmad; Madolangan, Jamaluddin
Narra J Vol. 5 No. 2 (2025): August 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i2.1317

Abstract

Stigma often accompanies people with multidrug-resistant tuberculosis (MDR-TB) and potentially affects their health-related quality of life (HRQoL). The aim of this study was to investigate the stigma faced by patients with MDR-TB, both from the patients' and community's perspective, and its relationship with HRQoL. Data was gathered at the provincial hospital in Makassar, South Sulawesi, Indonesia. The instrument employed in this research was the Indonesian version of the tuberculosis (TB) stigma instrument to assess MDR-TB stigma from the patient and community perspectives. The patient perspective represents how individuals with TB perceive and experience stigma, including the fear of disclosure, isolation, and guilt (feeling responsible for the burden on their family or their own risky behaviors). Meanwhile, the community perspective reflects how individuals with TB perceive societal attitudes towards them, such as social distancing, avoidance, and reluctance to interact. HRQoL was measured using the European quality of life-5 dimensions-5 level version (EQ-5D-5L) instrument. Notably, the evaluation of anxiety and depression is centered on the fifth dimension of the EQ-5D-5L instrument. A total of 210 patients with MDR-TB were included in the study, all of whom reported experiencing stigma. Most participants perceived stigma at a moderate level, with 76% from the patient perspective and 71% from the community perspective. The average EQ-5D-5L index score was 0.72 (95% confidence interval (95%CI): 0.68–0.76). Measurements from both perspectives show similar scores. There is a substantial negative association between the level of stigma and HRQoL, both from the patient's perspective (R2=-0.33; F=102.52; p<0.001) and the community's (R2=-0.32; F=96.76; p<0.001). The study highlights that the stigma of MDR-TB significantly affects the HRQoL from the patient and community perspective.
Co-Authors . Lukman . Mufidah . Subehan A. Prasetiyo, Nugraha Ahmad Fuady Aisyah Fatmawaty Aliyah Aliyah Alkaff, Sylmina D. Amin, Irnawati H. Andi Anggriani Ardiansyah, Akhmad Arif Santoso Asniar Pascayantri Bahar, Muhammad A. Bapulo, Nurdewi N. Bustanul Arifin Cangara, Husni Chaeratunnisa, Rizkya Dewita Fatiah Djaharuddin, Irawaty Dwi Leboe Ermina Pakki Fatmawati Fatmawati Firzan Nainu Gatot S Lawrence Gatot S. Lawrence Gemini Alam Habibie Habibie Hakim, Azima Hardiana, Iyan Hardiyanti, Widya Hasteti Husni - Herlina Rante Herlina Rante Iman Setiawan Inda Pratiwi Indriani Indriani Irawan Yusuf Ismail Ismail Isriany Ismail Jumriani Tammas Kasim, Hasyim Khairuddin - Latada, Nadila P. Latifah Rahman Latifah Rahman M Natsir Djide M Rusdi M. Natsir Djide Madolangan, Jamaluddin Mae Sri Hartati Wahyuningsih Marianti A Manggau Massi, Muhammad N. Mirianti A. Manggau, Mirianti A. Mudjahid, Mukarram Mufidah Mufidah Muh. Akbar Bahar Muhammad Aswad Munawarah Munawarah Munawwaroh, Makiyatul Nasruddin AM Niamuzisilawati, Eva Nurhikma A Pieter, Nova Audrey Luetta Pratama, Muhammad R. Pratiwi, Nadya Purba, Fredrick D. Putri, Tenri ZAD. Rina Agustina Risfah Yulianty Risfah Yulianty Risfah Yulianty Rosany tayeb Sanusi, Mayamariska Sarifah, Latifah M. Sartini Sartini Sarwadan, Muhamad G. Sri Yolandari Sumarheni Sumarheni Sunarti Syamsu Nur, Syamsu Tadjuddin Naid Tuti Handayani Zainal Umar, Abdul H. Utami, Ikrimah N. Veni Hadju Yayuk S. Rahayu Yulia Yusrini Djabir Yusnita Rifai Yuyun Sulitiani Zhulhajsyirah Zhulhajsyirah