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Journal : Biosaintifika: Journal of Biology

Direct Organogenesis of Different Explants of Aceh Patchouli (Pogostemon cablin Benth.) with Several BAP Concentrations Prayoga, Mohammad Candra; Soeparjono, Sigit; Dewanti, Parawita; Handoyo, Tri; Hardjo, Popy Hartatie; Restanto, Didik Pudji
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 3 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i3.12839

Abstract

The patchouli plant (Pogostemon cablin Benth.) is a tropical herbaceous plant that produces essential oil. One of the problems is that the production is not yet optimal. Fulfillment of superior seedlings can help increase patchouli productivity. Conventional patchouli propagation through stem cuttings is ineffective and takes longer. Patchouli propagation can be done using a tissue culture approach via direct organogenesis to produce seedlings quickly and efficiently. Effective patchouli propagation methods and successful acclimatization are very important to research to support the propagation and breeding of patchouli plants. The aim of this research was to determine the best of BAP concentration in direct organogenesis of leaf and stem explants. The research design used a completely randomized series of hormone BAP, it has 5 levels, namely 0 mg/L (as control), 0.25 mg/L, 0.50 mg/L, 0.75 mg/L, and 1.0 mg/L. The explants used were the leaves and stems of Aceh patchouli. Plantlets are acclimatized in compost media and covering treatment. Based on the results of observations, the best BAP concentration is 0.25 mg/L with the initial observation parameters of the early emergence of shoots, number of shoots, and length of shoots on leaf explants were 10 daps, 35.33 shoots, and 2.83 cm respectively. The use of leaf explants showed a better response compared to stem explants. Patchouli plantlets were successfully acclimatized and can adapt to the ex vitro environment using the covering method. Successful patchouli propagation and high acclimatization can help produce effective patchouli seeds.
Co-Authors . Usmadi afandi, subhan affan Ahnaf, Yusuf Dary Alfarisy, Fariz Kustiawan Alfarizi, Zulfikar Ahmad Almansyah Nur Sinatrya Anang Syamsunihar Arie Mudjiharjati ARIE MUDJIHARJATI Aultavia, Reza Novita Azmi Saleh Azmi, Hilmi Tsabiatul Bagus Tripama Bakri, Faizal Bintaro, Jatmiko Hardi Bintoro, Jatmiko Hardi Budi Kriswanto Budi Kriswanto Budi Kriswanto, Budi Budinuljanto Zainul, Luhur Alif Cahyoadi Bowo Chozin, dan Muhammad Dela Kurniasari Devi Yulianti Dwi Erwin Kusbianto Dyta Romadhona Purwasita Evita Soliha Hani Fachryansyah, Moh Faizal, Risky Febby Mardhiana Febery Hery Suandana Habriantono, Bakhroini Harsanti, Restiani Sih Imam Taufik Imam Taufik Ketut Anom Wijaya Khozim, Mohammad Nur Khozin, M. Nur Khozin, Mohammad Nur Kiptiyah, Mariatul Kriswanto, Budi Laily Ilman Widuri, Laily Ilman Laksono Trisnantoro Lenny Widjayanthi Listya Purnamasari Maknuna, Luk Luil Miftachul Hudah Moh. Syahrul Munir Mohammad Nur Khozim Mohammad Nur Khozin Mona, Muhammad Dima Say Muhammad Dima Say Mona Muhammad Hazmi Munandar, Denna Eriani Nada Nur Rizkiyah Niagra, Risnanda Deva Martha Nisa Budi Arifiana Nur Khozin, Mohammad Parawita Dewanti Popy Hartatie Hardjo Prayoga, Mohammad Candra Priyo Sugeng Winarto Puguh Catur Wicaksana Puguh Catur Wicaksana, Puguh Catur Putri, Widya Kristyanti Raden Soedradjad Rahmawati Rahmawati Raihan Hidayatullah Ratna Sari Pratiwi Restanto, Didik Restanto, Didik Pudji Resti, Ricke Windi Risky Faizal Rizky Harikurniawan Rofikin, Mochammad Zaini Rofiqi, Ahmad S, Setiyono Sa'diyah, Halimatus Sandy Al Firdauzi Setiawati, Tri Candra Sherly Meirdania Lestari Sholeh Avivi Slameto Slameto Slameto Soeharto Sri Hartatik Suharto .... Suliswanto, Eko Nur Tri Agus Siswoyo Tri Agus Siswoyo Tri Candra Setiawati TRI HANDOYO Tri Handoyo Tri Saputra, Tri Tri Wahyu Saputra Wagiyana Widya Kristiyanti Putri Wildan Muhlison, Wildan Wulanjari, Distiana Yuli Hariyati Yulianto, Roni