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Journal : Journal of Tropical Crop Science

The Potentials of Limau (Citrus amblycarpa Hassk. Ochse) as A Functional Food and Ornamental Mini Tree Based on Metabolomic and Morphological Approaches Budiarto, Rahmat; Poerwanto, Roedhy; Santosa, Edi; Efendi, Darda
Journal of Tropical Crop Science Vol 4 No 2 (2017): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (440.628 KB) | DOI: 10.29244/jtcs.4.2.49-57

Abstract

Limau (Citrus amblycarpa (Hassk.) Ochse) is one of the native Citrus species of West Java that is grown in the local backyard, thus is easily found in local cuisines. Limau has great potentials for use as a potted plant in home gardens for its aesthetic value and fragrance. The objective of this research was to describe the metabolites fingerprint and morphology of Limau as a potential functional and ornamental mini tree. Methanol leaf extracts from one-year-old Limau originated from Bogor were analyzed using untargeted Gas Chromatography Mass Spectrometry (GCMS). Methanol leaf extract was dominated by vitamin E and composed of other 18 metabolites including phytosterols, fatty acids and terpenes. The major portion of fruit extract consisted of 6-octadecenoic acid, palmitate and various fragrance compounds such as alpha sinensal, alpha limonene, beta citronellal, citronellol, and sabinene. Limau fruits are also a functional food due to its rich antioxidant and aromatic content in the fruits. The morphology of limau tree is suitable as a potted ornamentals or mini-fruits tree for its small crown, sparse branching with unifoliate aromatic leaves and small spines, and plentiful aromatics mini fruits with the diameter of around 2-4 cm.  
A Review of Root Pruning to Regulate Citrus Growth Budiarto, Rahmat; Poerwanto, Roedhy; Santosa, Edi; Efendi, Darda
Journal of Tropical Crop Science Vol 6 No 01 (2019): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (462.852 KB) | DOI: 10.29244/jtcs.6.01.1-7

Abstract

Root is the important plant organ hiding below the soil that serves as plant supporting structure, water and nutrient supplier and assimilates storage. Root growth occurs in turn with shoot part, forming repeated cyclic pattern because of the photosynthate competition. Root growth regulation is one of the promising practices to boost the yield of fruit tree, especially citrus that is economically important and highly demanded fruit. Root pruning varied based on the scale of intensity and plant growth stage, including air root pruning, knife root pruning and modern pruning using root pruner machine mounted to a tractor. Root pruning to induce the growth of more fi brous root system and to correct root deformation at seedling stage have potentials to improve post-transplant growth. Besides, it has a potential to replace the use of chemical plant growth regulator for a more environmentally friendly practice. The pruning of root impeded the canopy growth, altered the plant water status leading to fl ower promotion. In addition, it is also a promising practice to overcome the alternate bearing on fruit tree, including citrus, through the suppressing of excessive growth and restricting the high fruit load during the ‘on year’ and allowing a better carbohydrate storage for the improvement of yield during the ‘off year’.
Production, Post-Harvest and Marketing of Kaffir Lime (Citrus hystrix DC) In Tulungagung, Indonesia Budiarto, Rahmat; Poerwanto, Roedhy; Santosa, Edi; Efendi, Darda; Agusta, Andria
Journal of Tropical Crop Science Vol 6 No 02 (2019): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (719.259 KB) | DOI: 10.29244/jtcs.6.02.138-143

Abstract

Kaffir lime (Citrus hystrix DC) is one of the Citrus species that is popular as food spices in Indonesia. This study was aimed at describing the production system, post-harvest handling, marketing and evaluating the product variation of kaffir lime cultivated in the Tulungagung district, East Java Province, Indonesia. Data was collected through a survey by interviewing kaffir lime farmers, distributors and customers in June 2018. Kaffir lime is cultivated by local farmers using traditional and conventional agriculture practices. The farmer used good quality seedlings as planting materials and applies animal manure or compost to the trees. Kaffir lime is grown on rain-fed land; fertilizers and pesticides are applied sparingly. The kaffir lime leaves could be harvested twice a year in different seasons. Better leaf quality can be obtained during the rainy seasons. The leaf production per tree is estimated at about 100 gram per plant per season. Post-harvest handling involved sorting, packing and marketing without any cooling treatment. The standard gate price of kaffir lime leaf in June 2019 was IDR 6,000-7,000, IDR 9,000-10,000 at the collector level, IDR 11,000-12,000 at wholesaler, and IDR 14,000-16,000 at small retailers. Leaves were distributed to traditional market in Tulungagung, neighbouring districts such as Kediri, Trenggalek and Blitar, and also to capital cities such Jakarta. The main product of kaffir lime is the fresh leaf for food seasoning and as a raw material to produce essential oil and dry leaf powder. The stems have been used for fuel in home industries. The fruits of kaffir lime are rarely found in the market and have great potentials for essential oil production and recently, a promising commodity for export.
Study of Incubation Duration After Degreening and Storage Methods on the Quality of Tangerine "Garut" (Citrus reticulata B.) Tiara, Dede; Poerwanto, Roedhy; Efendi, Darda
Journal of Tropical Crop Science Vol 6 No 03 (2019): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (358.35 KB) | DOI: 10.29244/jtcs.6.03.193-200

Abstract

Ripened citrus fruits with an orange rind have a higher market demand relative to unripen citrus with a green rind. This research was aimed to evaluate the combination of post-degreening incubation duration before storage with the type of storage to increase orange rind color and storage life of citrus fruit. A randomized block design with two factors was used; the first factor was post-degreening incubation duration before storage that comprised of without incubation, incubation for two and four days. The second factor was storage method that comprised of storage at room temperature (29 ± 1oC), at 18 ± 1oC, waxing and storage at room temperature, waxing and storage at 18 ± 1oC. The result shows that post-degreening incubation duration that increased orange color of citrus rind was four days. The citrus which was stored at 18 ± 1oC had the smallest fruit weight loss. The best treatment combination which increased orange color formation and had lower percentage of fruit weight loss was a combination of 4 days incubation after degreening and storage at 18± 1oC.
Growth Response, Physiology, Metabolomic, and Production of Micro-Tom Tomatoes to Additional Lighting with White and Purple Light-Emitting Diode Lubis, Wahyu Muhammad Yuha; Hapsari, Dhika Prita; Poerwanto, Roedhy; Matra, Deden Derajat
Journal of Tropical Crop Science Vol. 12 No. 02 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.02.284-295

Abstract

Tomatoes are a widely produced and consumed fruit-vegetable belonging to the Solanaceae family. It contains minerals, vitamins, essential amino acids, sugars, and fiber. Micro-Tom is a wild-type cultivar from a cross between the Florida Basket and Ohio 4013-3 cultivars. Micro-Tom has two mutant types, the iaa9-3 and iaa9-5 mutants, which exhibit strong parthenocarpic properties. It is classified as a mutant tomato due to a mutation in the IAA9 gene, which belongs to the Auxin/IAA (Indole-3-Acetic-Acid) gene family and plays a role in suppressing the endogenous auxin signal transcription pathway. Using artificial light in cultivation techniques shortens the plant cycle and accelerates the juvenile phase. This study aimed to investigate the morphological, physiological, and production responses of Micro-Tom tomatoes to supplemental LED lighting. The research was conducted at the Leuwikopo experimental field of IPB University from March to November 2023. A completely randomized design was employed, with two factors (LED spectrum and Micro-Tom genotyping) and nine replicates. The study also examined the potential benefits of combining polychromatic and monochromatic light-emitting diodes (LEDs). The response of Micro-Tom tomatoes to artificial lighting with purple and white LEDs did not show significant differences in growth and production parameters. However, significant differences were observed between the tomato types, specifically between the Wild-type and the iaa9-3 mutant. No significant differences were found in LED treatment or Micro-Tom genotype for physiological parameters such as chlorophyll content and glucose-fructose levels. Regarding fruit quality, no significant differences were observed for parameters such as total soluble solids (TSS, Brix) to total titratable acidity (TTA, acidity), glucose and fructose, and malic acid. The LC-MS/MS analysis of leaves exposed to purple LED light revealed a profile dominated by secondary metabolites from the fatty acid compound group, suggesting the potential benefits of this lighting combination. In conclusion, using LED lights can accelerate the plant life cycle and shorten the juvenile phase, as evidenced by the first flower emergence, which occurred 20 days after transplanting (DAT) in the wild-type genotype and 16 DAT in the mutant genotype.
Morphophysiological Study of Kecapi (Sandoricum koetjape Merr.) Seedlings Against Different Artificial Light Spectra and Intensities Defitrianida, Asyhuriyah Wardah; Poerwanto, Roedhy; Hapsari, Dhika Prita; Matra, Deden Derajat
Journal of Tropical Crop Science Vol. 12 No. 03 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.03.604-614

Abstract

Kecapi (Sandoricum koetjape Merr.) is a tropical fruit species that belongs to the Meliaceae family. Kecapi trees require 5-7 years to produce fruit when grown from seeds, necessitating efforts to accelerate their growth, including through the modification of light. This research aimed to study kecapi’s morphological, physiological, and anatomical responses to light spectra and intensities from light-emitting diodes. The experiment was designed using a two-factor, completely randomized design (CRD), namely LED light spectrum (white and purple) and light intensity (2 and 4 lights). The result showed that morphological and vegetative growth were significantly affected under the high-intensity purple LED treatment. The low-intensity purple LED treatment increased stomatal conductance and chlorophyll-b synthesis. The transpiration rate was highest under the high intensity white LED treatment. Low-intensity white LED treatment only increased the abaxial stomatal aperture. As the intensity increased, it also increased N-Total content but decreased micronutrient levels in the leaves.
Sensory Evaluation of the Quality of Kaffir Lime (Citrus hystrix DC.) Leaves Exposed to Different Postharvest Treatments Budiarto, Rahmat; Poerwanto, Roedhy; Santosa, Edi; Efedi, Darda; Agusta, Andria
Journal of Tropical Crop Science Vol. 8 No. 02 (2021): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.8.02.71-79

Abstract

This study aimed to evaluate the sensory attributes such as aroma, color and texture of kaffir lime leaves in response to various post-harvest treatment. The hedonic test approach was conducted by inviting 70 untrained panelists on seven post-harvest treatments, i.e. (D1) post-sortation fresh leaves, (D2) pre-sortation fresh leaves, (D3) cold-storage leaves, (D4) low temperature-storage leaves, (D5) brown dry leaves, (D6) fresh leaf-flour, and (D7) brown dry leaf-flour. The result showed that most of panelist agreed that aroma was the most important quality attribute that determined the level of preference in kaffir lime leaf products. Among seven tested products, the aroma, color, texture and overall impression of D1 was the most favorite one, while D4, D5, and D7 were assessed as low preference products. This work showed the importance of sortation and cold storage to maintain consumer likeliness. The criteria for sortation were green, clean, pest-disease free, scar free, high uniformity and fresh condition of leaves. Cold-storage at -20oC maintained the color and texture of kaffir lime leaves better than low temperature storage (5oC). To make leaf flour, the use of fresh green leaves was significantly better than brown dry ones. This finding might become the baseline data for the development of kaffir lime leaf product in the future.
The Effects of Preharvest Mild Shading on the Quality and Production of Essential Oil from Kaffir Lime Leaves (Citrus hystrix) Budiarto, Rahmat; Poerwanto, Roedhy; Santosa, Edi; Efendi, Darda; Agusta, Andria
Journal of Tropical Crop Science Vol. 9 No. 01 (2022): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.9.01.15-21

Abstract

Kaffir lime (Citrus hystrix DC) is a less popular citrus species commonly used as a food spice and a source of essential oil. Early studies report the success of preharvest mild shading to increase leaf yield, although there is still limited information on the effect of preharvest shading on the quality of essential oil produced. The aim of this current study is to evaluate the effect of preharvest mild shading factors on the yield, physical characteristics, and metabolite fingerprinting of kaffir lime leaves essential oil (KLLEO). One-year-old kaffir lime trees were sampled in two preharvest treatments, i.e., open sun and mild shading (24% light reduction) at Pasir Kuda experimental field, Bogor, Indonesia. Statistical analysis showed that there was no significant effect of preharvest treatment on yield and physical characteristics (color, specific gravity, and refractive index) of KLLEO. In contrast, there was a metabolite fingerprinting variation of KLLEO as an effect of mild shading. The relative percentage of bergamol, citronellol, caryophyllene oxide, citronellic acid, isopulegol, isopulegyl formate, limonene, linalool, and linalool oxide was increased by mild shading. On the other hand, the main metabolite (citronellal) was significantly reduced by about 10% in shading treatment, as compared to the open-sun ones.
Yield and Physicochemical Characteristics of Kaffir Lime Leaf Essential Oils Subjected to Different Post-Harvest Treatment Budiarto, Rahmat; Poerwanto, Roedhy; Santosa, Edi; Efendi, Darda; Agusta, Andria; Rofiq, Muhamad Abdul
Journal of Tropical Crop Science Vol. 11 No. 02 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.02.97-104

Abstract

The importance of kaffir lime leaf as essential oils (EOs) raw material is starting to get attention because of its commercial value; however, there is no quality reference for kaffir lime leaf EOs, especially in response to various post-harvest handlings. This study aimed to describe the physicochemical characteristics and yield of kaffir lime EOs subjected to different post-harvest. Bogor originated-kaffir lime leaf was prepared to be subjected to several post-harvest treatments, i.e., control/fresh green leaves (P1); milling to produce green leaf flour (P2); drying to produce dry brown leaf (P3), and milling and drying to produce brown leaf flour (P4). The result showed that post-harvest treatment generally decreases an oil yield and increases darkness color, specific gravity and refractive index of tested EOs. Additionally, post-harvest treatment also changes metabolite profile revealed by GCMS analysis. The relative percentage of caryophyllene and citronellol tends to increase, while the linalool and citronellal levels decrease due to tested post-harvest treatment. It was implied that for the benefit of the fragrance industry with a high citronellal requirement, EOs should be made from fresh green leaves and the leaves should be avoided from drying and powdering treatment.
Co-Authors , Dorly , Sakhidin , Yudiwanti . DARMAWAN . SAMANHUDI . SUHARSONO Abdul Munif Achmad Surkati Ade Wachjar Agus Purwito Ahmad Ghozi Manshuri Ahmad Ghozi Mansyuri Ahmad S. Abidin Ahmad Sutopo Akmal, Ajmir ALI NURMANSYAH Alifiya Herwitarahman Anas D Susila Anas Dinurrohman Susila Andria Agusta ANDRIA AGUSTA Ansyori, Ansyori Aris Purwanto Asmini Budiani Bambang S . Purwoko Bambang S. Purwoko Bambang Sapta Purwoko Cenra Intan Hartuti Tuharea CICIK SURIANI D Fatria D Fatria Deden Derajat Matra Defitrianida, Asyhuriyah Wardah Dewi Sukma Dhika Prita Hapsari Dian Fahrianty Didy Sopandie Djoko Santoso Dorly Dorly E Efendi Edi Santosa Edi Santosa Edi Santosa Efendi, Darda EKO SETIAWAN Eko Setiawan Endah Retno Palupi Endang Gunawan Faqih Udin Fauziyah Harahap Fumio Fukuda HAJRIAL ASWIDINNOOR Hanifah Muthmainnah Hartrisari Hardjomidjojo Hiroshi Inoue I Hidayati I MADE ARTIKA I NYOMAN RAI Ilmi, Nadhirah Karimatul Iman Rusmana Inanpi Hidayati Sumiasih, Inanpi Hidayati INDAH WULANDARI Indah Wulandari Irsyad Maulana Iskandar Lubis Jawal Muhammad Anwarudinsyah Juanasri Juanasri JULIARNI JULIARNI Kasutjianingati . Ketty Suketi Kuniyuki Saitoh La Ode Safuan Laksono Trisnantoro Latifah K. Darusman Latifah Kosim Darusman Lizawati . Lubis, Wahyu Muhammad Yuha Lukman Liferdi Machfud Machfud Marimin Marimin Matra, Deden Derajat Maulana, Mohamad Akhbar Memen Surahman Mohamad Akhbar Maulana Mohamad Rahmad Suhartanto Muhamad Noor Azizu, Muhamad Noor Muhammad Arif Nasution Muhammad Darmawan Musdalifah, Nuzlul Naohiro Kubota Nian Rimayanti H. Nobuo Sugiyama Nono Sutrisno Nono Sutrisno Nur Wahyu Sariningtias Nurfitri Ramadhani Nurul Khumaida Odit Ferry Kurniadinata Qadir, Abdul Rahayu, Resa Sri Rahmat Budiarto Ramdan Hidayat Rd. Selvy Handayani Resa Sri Rahayu Resa Sri Rahayu Retno Astuti Riana Jumawati Rofiq, Muhamad Abdul Roza Yunita S Susanto Santun R.P. Sitorus Septirosya, Tiara Slamet Susanto Soaloon Sinaga Sobir Sobir SOEKISMAN TJITROSEMITO Sri Astuti Rais Sri Yuliani Sri Yuliani SRIANI SUJIPRIHATI Sriani Sujiprihati Suci Rahayu Sudarsono Sudirman Yahya Suryo Wiyono Sutrisno, Sutrisno T Purnama Tanari, Yulinda Taruna Shafa Arzam Taruna Shafa Arzam, Taruna Shafa TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tetty CHAIDAMSARI Tiara, Dede Titin Purnama Tri Muji Ermayanti Trikoesoemaningtyas Vandra Kurniawan Wa Ode Muliastuty Widya Sari Winarso D. Widodo Y A Purwanto Y A Purwanto Yandra Arkeman Yundari, Yundari Yunus, Ismadi