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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Natur Indonesia Journal of Tropical Soils Agrovigor: Jurnal Agroekoteknologi Jurnal Hortikultura JURNAL AGROTEKNOLOGI Jurnal Agroteknos Warta IHP (Warta Industri Hasil Pertanian) International Journal of Applied Biology Jurnal AGROSAINS dan TEKNOLOGI Journal of Tropical Crop Science Journal of Tropical Horticulture Jerami Menara Perkebunan Jurnal Hexagro Media Pertanian Jurnal Manusia dan Lingkungan International Research Journal of Business Studies
Roedhy Poerwanto
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (IPB University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Energy Environmental Science Industrial & Manufacturing Engineering Medicine & Pharmacology Social Sciences

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Journal : Menara Perkebunan

 1 
Ekspresi fenotipe gen APETALA1 kakao (TcAP1) pada eksplan tembakau Phenotypic expression of cacao APETALA1 (TcAP1) in tobacco explant Tetty CHAIDAMSARI; . SAMANHUDI; Asmini BUDIANI; Roedhy POERWANTO; Djoko SANTOSO
E-Journal Menara Perkebunan Vol 74, No 1: Juni 2006
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1665.837 KB) | DOI: 10.22302/iribb.jur.mp.v74i1.116

Abstract

Summary APETALA1 (AP1) is one of flowering identity genes that determines the formation of sepal and petal tissues. An AP1 homologue was cloned from cacao flowers by bio-techniques coupled with bio-informatics. Examination of phenotypic expression was conducted with transgenesis of the 35S-TcAP1 construct using leaf disk technique of tobacco leaf explants mediated by Agrobacterium tumefaciens. PCR specific to TcAP1 demonstrated that the technique is effective in introducing the 35S-TcAP1 construct into tobacco plant cells. RT-PCR with total RNA from the leaves of transgenic tobacco plantlets showed that expression levels of the TcAP1 events varied. The variation of the transcript levels was comparable to the morphological phenotype of the tobacco plantlets grown in vitro. The cultures expressing TcAP1 at moderate levels, have developed into intact plantlets and set up flowers in vitro.Ringkasan APETALA1 (AP1) diketahui merupakan salah satu gen identitas pembungaan yang mengendalikan terbentuknya jaringan sepal dan petal. Homolog AP1 telah diklon dari organ bunga kakao (TcAP1) dengan kombinasi bio-techniques dan bio-informatics. Pengujian ekspresi fenotipe TcAP1 dilakukan dengan transgenesis konstruk konstitutif 35S-TcAP1 menggunakan teknik leaf disk eksplan daun tembakau dan mediasi Agrobacterium tumefaciens. Pengujian PCR spesifik TcAP1 menunjukkan bahwa teknik tersebut cukup efektif dalam mengintroduksikan konstruk 35S-TcAP1 ke dalam sel tanaman tembakau. RT-PCR dari daun planlet tembakau trangenik membuktikan bahwa tingkat ekspresi TcAP1 tersebut bervariasi. Perbedaan level ekspresi TcAP1 ini memberikan pengaruh yang nampak sebanding terhadap perkembangan morfologis planlet tembakau in vitro.  Kultur yang mengekspresikan TcAP1 pada level sedang mampu beregenerasi menjadi planlet sempurna dan membentuk bunga in vitro.
Ekspresi fenotipe gen APETALA1 kakao (TcAP1) pada eksplan tembakau Phenotypic expression of cacao APETALA1 (TcAP1) in tobacco explant Tetty CHAIDAMSARI; . SAMANHUDI; Asmini BUDIANI; Roedhy POERWANTO; Djoko SANTOSO
Menara Perkebunan Vol. 74 No. 1: 74 (1), 2006
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v74i1.116

Abstract

Summary APETALA1 (AP1) is one of flowering identity genes that determines the formation of sepal and petal tissues. An AP1 homologue was cloned from cacao flowers by bio-techniques coupled with bio-informatics. Examination of phenotypic expression was conducted with transgenesis of the 35S-TcAP1 construct using leaf disk technique of tobacco leaf explants mediated by Agrobacterium tumefaciens. PCR specific to TcAP1 demonstrated that the technique is effective in introducing the 35S-TcAP1 construct into tobacco plant cells. RT-PCR with total RNA from the leaves of transgenic tobacco plantlets showed that expression levels of the TcAP1 events varied. The variation of the transcript levels was comparable to the morphological phenotype of the tobacco plantlets grown in vitro. The cultures expressing TcAP1 at moderate levels, have developed into intact plantlets and set up flowers in vitro.Ringkasan APETALA1 (AP1) diketahui merupakan salah satu gen identitas pembungaan yang mengendalikan terbentuknya jaringan sepal dan petal. Homolog AP1 telah diklon dari organ bunga kakao (TcAP1) dengan kombinasi bio-techniques dan bio-informatics. Pengujian ekspresi fenotipe TcAP1 dilakukan dengan transgenesis konstruk konstitutif 35S-TcAP1 menggunakan teknik leaf disk eksplan daun tembakau dan mediasi Agrobacterium tumefaciens. Pengujian PCR spesifik TcAP1 menunjukkan bahwa teknik tersebut cukup efektif dalam mengintroduksikan konstruk 35S-TcAP1 ke dalam sel tanaman tembakau. RT-PCR dari daun planlet tembakau trangenik membuktikan bahwa tingkat ekspresi TcAP1 tersebut bervariasi. Perbedaan level ekspresi TcAP1 ini memberikan pengaruh yang nampak sebanding terhadap perkembangan morfologis planlet tembakau in vitro.  Kultur yang mengekspresikan TcAP1 pada level sedang mampu beregenerasi menjadi planlet sempurna dan membentuk bunga in vitro.
Co-Authors , Dorly , Sakhidin , Yudiwanti . DARMAWAN . SAMANHUDI . SUHARSONO Abdul Munif Achmad Surkati Ade Wachjar Agus Purwito Ahmad Ghozi Manshuri Ahmad Ghozi Mansyuri Ahmad S. Abidin Ahmad Sutopo Akmal, Ajmir ALI NURMANSYAH Alifiya Herwitarahman Anas D Susila Anas Dinurrohman Susila Andria Agusta ANDRIA AGUSTA Ansyori, Ansyori Aris Purwanto Asmini Budiani Bambang S . Purwoko Bambang S. Purwoko Bambang Sapta Purwoko Cenra Intan Hartuti Tuharea CICIK SURIANI D Fatria D Fatria Deden Derajat Matra Defitrianida, Asyhuriyah Wardah Dewi Sukma Dhika Prita Hapsari Dian Fahrianty Didy Sopandie Djoko Santoso Dorly Dorly E Efendi Edi Santosa Edi Santosa Edi Santosa Efendi, Darda EKO SETIAWAN Eko Setiawan Endah Retno Palupi Endang Gunawan Faqih Udin Fauziyah Harahap Fumio Fukuda HAJRIAL ASWIDINNOOR Hanifah Muthmainnah Hartrisari Hardjomidjojo Hiroshi Inoue I Hidayati I MADE ARTIKA I NYOMAN RAI Ilmi, Nadhirah Karimatul Iman Rusmana Inanpi Hidayati Sumiasih, Inanpi Hidayati Indah Wulandari INDAH WULANDARI Irsyad Maulana Iskandar Lubis Jawal Muhammad Anwarudinsyah Juanasri Juanasri JULIARNI JULIARNI Kasutjianingati . Ketty Suketi Kuniyuki Saitoh La Ode Safuan Laksono Trisnantoro Latifah K. Darusman Latifah Kosim Darusman Lizawati . Lubis, Wahyu Muhammad Yuha Lukman Liferdi Machfud Machfud Marimin Marimin Matra, Deden Derajat Maulana, Mohamad Akhbar Memen Surahman Mohamad Akhbar Maulana Mohamad Rahmad Suhartanto Muhamad Noor Azizu, Muhamad Noor Muhammad Arif Nasution Muhammad Darmawan Musdalifah, Nuzlul Naohiro Kubota Nian Rimayanti H. Nobuo Sugiyama Nono Sutrisno Nono Sutrisno Nur Wahyu Sariningtias Nurfitri Ramadhani Nurul Khumaida Odit Ferry Kurniadinata Qadir, Abdul Rahayu, Resa Sri Rahmat Budiarto Ramdan Hidayat Rd. Selvy Handayani Resa Sri Rahayu Resa Sri Rahayu Retno Astuti Riana Jumawati Rofiq, Muhamad Abdul Roza Yunita S Susanto Santun R.P. Sitorus Septirosya, Tiara Slamet Susanto Soaloon Sinaga Sobir Sobir SOEKISMAN TJITROSEMITO Sri Astuti Rais Sri Yuliani Sri Yuliani Sriani Sujiprihati SRIANI SUJIPRIHATI Suci Rahayu Sudarsono Sudirman Yahya Suryo Wiyono Sutrisno, Sutrisno T Purnama Tanari, Yulinda Taruna Shafa Arzam Taruna Shafa Arzam, Taruna Shafa TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tetty CHAIDAMSARI Tiara, Dede Titin Purnama Tri Muji Ermayanti Trikoesoemaningtyas Vandra Kurniawan Wa Ode Muliastuty Widya Sari Winarso D. Widodo Y A Purwanto Y A Purwanto Yandra Arkeman Yundari, Yundari Yunus, Ismadi