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Journal : International Journal of Applied Biology

The Analysis of β-cryptoxanthin and Zeaxanthin using HPLC in the Accumulation of Orange Color on Lowland Citrus Sumiasih, Inanpi Hidayati; Poerwanto, Roedhy; Efendi, Darda; Agusta, Andria; Yuliani, Sri
International Journal of Applied Biology Vol 1, No 2 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Citrus peel color is one of the main quality attributes which was caused by the accumulation of carotenoids and its derivatives, especially β-citraurine. It makes citrus peel color looks attractive (orange). The orange color is a mixture of β-cryptoxanthin with β-citraurin. The objectives of this study were (1) to observe the effect of precooling and duration of proper ethylene exposure in the formation of orange color on citrus peel, (2) to identify and determine the β-cryptoxanthin content and total chlorophyll on citrus peel. Citrus was from Tuban, East Java while the study was conducted at PKHT IPB and LIPI. Precooling and without precooling treatment prior to injection of 100 ppm of ethylene exposed at 15 °C, duration of exposure control (0), 24, and 48 hours. The results show that the best color of the Citrus Color Index (CCI) is the precooling treatment and the duration of ethylene exposure for 24 hours, which can reduce total chlorophyll content about 8 times and proved to increase β-cryptoxanthin pigment content five times in accelerating the formation of orange citrus reticulata peel color to bright orange. Degreening has no significant effect on total dissolved solids and the firmness level of citrus fruits.Keyword: β-cryptoxhantin; citrus; chlorophyll; degreening; ethylene zeaxanthin.
The Analysis of β-cryptoxanthin and Zeaxanthin using HPLC in the Accumulation of Orange Color on Lowland Citrus Inanpi Hidayati Sumiasih; Roedhy Poerwanto; Darda Efendi; Andria Agusta; Sri Yuliani
International Journal of Applied Biology Vol. 1 No. 2 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v1i2.3066

Abstract

Citrus peel color is one of the main quality attributes which was caused by the accumulation of carotenoids and its derivatives, especially β-citraurine. It makes citrus peel color looks attractive (orange). The orange color is a mixture of β-cryptoxanthin with β-citraurin. The objectives of this study were (1) to observe the effect of precooling and duration of proper ethylene exposure in the formation of orange color on citrus peel, (2) to identify and determine the β-cryptoxanthin content and total chlorophyll on citrus peel. Citrus was from Tuban, East Java while the study was conducted at PKHT IPB and LIPI. Precooling and without precooling treatment prior to injection of 100 ppm of ethylene exposed at 15 °C, duration of exposure control (0), 24, and 48 hours. The results show that the best color of the Citrus Color Index (CCI) is the precooling treatment and the duration of ethylene exposure for 24 hours, which can reduce total chlorophyll content about 8 times and proved to increase β-cryptoxanthin pigment content five times in accelerating the formation of orange citrus reticulata peel color to bright orange. Degreening has no significant effect on total dissolved solids and the firmness level of citrus fruits.Keyword: β-cryptoxhantin; citrus; chlorophyll; degreening; ethylene zeaxanthin.
Study of Several Stages of Maturity and Storage Temperature on Color Changes and Shelf Life of Mangosteen (Garcinia mangostana L.) Inanpi Hidayati Sumiasih; Roedhy Poerwanto; Darda Efendi
International Journal of Applied Biology Vol. 3 No. 1 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v3i1.5967

Abstract

Color and freshness of mangosteen are important characters as benchmarks for consumers in the selection and purchase of mangosteen in the market. Color, freshness and shelf life of mangosteen are affected by the stage of maturity at harvest and the correct storage temperature. Information about the correct maturity stage for harvesting and storage temperature of mangosteen are needed by the mangosteen farmers, local merchant, and exporters as an effort to maintain the quality of fresh product. The objective of this research was to study the effect of several maturity stage at harvest and storage temperature to mangosteen color changes and shelf life during storage. The research used Completely Randomized Design of two factors. The first factor was the fruit maturity stage at harvest consisting of: Maturity Stage 1, 2, 3 and 4. The second factor was storage temperature of 15 oC and room temperature. The result of harvesting mangosteen at Maturity Stage 1 could maintain skin color longer than at Maturity Stage 2, 3, and 4. Mangosteens that were harvested at Maturity Stage 1 and 2, combined with storage temperature of 15 oC could maintain fruit quality up to 30 days after harvest and could be used for export market. While harvesting at Maturity Stage 3 could maintain fruit quality up to 25 days after harvest and Maturity Stage 4 up to 20 days after harvest. Harvesting at Maturity Stage 4 followed by 15 oC storage temperature and all Maturity stages combined with room temperature storage could be used for local market.Keyword: fruit color; horticultural commodities; queen of tropical fruits; shelf life 
Co-Authors , Dorly , Sakhidin , Yudiwanti . DARMAWAN . SAMANHUDI . SUHARSONO Abdul Munif Achmad Surkati Ade Wachjar Agus Purwito Ahmad Ghozi Manshuri Ahmad Ghozi Mansyuri Ahmad S. Abidin Ahmad Sutopo Akmal, Ajmir ALI NURMANSYAH Alifiya Herwitarahman Anas D Susila Anas Dinurrohman Susila Andria Agusta ANDRIA AGUSTA Ansyori, Ansyori Aris Purwanto Asmini Budiani Bambang S . Purwoko Bambang S. Purwoko Bambang Sapta Purwoko Cenra Intan Hartuti Tuharea CICIK SURIANI D Fatria D Fatria Deden Derajat Matra Defitrianida, Asyhuriyah Wardah Dewi Sukma Dhika Prita Hapsari Dian Fahrianty Didy Sopandie Djoko Santoso Dorly Dorly E Efendi Edi Santosa Edi Santosa Edi Santosa Efendi, Darda EKO SETIAWAN Eko Setiawan Endah Retno Palupi Endang Gunawan Faqih Udin Fauziyah Harahap Fumio Fukuda HAJRIAL ASWIDINNOOR Hanifah Muthmainnah Hartrisari Hardjomidjojo Hiroshi Inoue I Hidayati I MADE ARTIKA I NYOMAN RAI Ilmi, Nadhirah Karimatul Iman Rusmana Inanpi Hidayati Sumiasih, Inanpi Hidayati Indah Wulandari INDAH WULANDARI Irsyad Maulana Iskandar Lubis Jawal Muhammad Anwarudinsyah Juanasri Juanasri JULIARNI JULIARNI Kasutjianingati . Ketty Suketi Kuniyuki Saitoh La Ode Safuan Laksono Trisnantoro Latifah K. Darusman Latifah Kosim Darusman Lizawati . Lubis, Wahyu Muhammad Yuha Lukman Liferdi Machfud Machfud Marimin Marimin Matra, Deden Derajat Maulana, Mohamad Akhbar Memen Surahman Mohamad Akhbar Maulana Mohamad Rahmad Suhartanto Muhamad Noor Azizu, Muhamad Noor Muhammad Arif Nasution Muhammad Darmawan Musdalifah, Nuzlul Naohiro Kubota Nian Rimayanti H. Nobuo Sugiyama Nono Sutrisno Nono Sutrisno Nur Wahyu Sariningtias Nurfitri Ramadhani Nurul Khumaida Odit Ferry Kurniadinata Qadir, Abdul Rahayu, Resa Sri Rahmat Budiarto Ramdan Hidayat Rd. Selvy Handayani Resa Sri Rahayu Resa Sri Rahayu Retno Astuti Riana Jumawati Rofiq, Muhamad Abdul Roza Yunita S Susanto Santun R.P. Sitorus Septirosya, Tiara Slamet Susanto Soaloon Sinaga Sobir Sobir SOEKISMAN TJITROSEMITO Sri Astuti Rais Sri Yuliani Sri Yuliani Sriani Sujiprihati SRIANI SUJIPRIHATI Suci Rahayu Sudarsono Sudirman Yahya Suryo Wiyono Sutrisno, Sutrisno T Purnama Tanari, Yulinda Taruna Shafa Arzam Taruna Shafa Arzam, Taruna Shafa TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tetty CHAIDAMSARI Tiara, Dede Titin Purnama Tri Muji Ermayanti Trikoesoemaningtyas Vandra Kurniawan Wa Ode Muliastuty Widya Sari Winarso D. Widodo Y A Purwanto Y A Purwanto Yandra Arkeman Yundari, Yundari Yunus, Ismadi