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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Media Veteriner Jurnal Sain Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Ilmu Ternak Veteriner Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) ANNALES BOGORIENSES Jurnal Medik Veteriner Jurnal Kedokteran Hewan Jurnal Bioteknologi & Biosains Indonesia (JBBI) Annales Bogorienses Jurnal Bioteknologi & Biosains Indonesia
Wahono Esthi Prasetyaningtyas
Departemen Anatomi, Fisiologi Dan Farmakologi Fakultas Kedokteran Hewan Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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FROZEN SEMEN CHARACTERISTICS OF BALI BULL IN DIFFERENT AGE GROUPS Witri, Brilla Widya; Arifiantini, Iis; Prasetyaningtyas, Wahono Esthi; Karja, Ni Wayan Kurniani
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10087

Abstract

One factor that affects frozen semen characteristics is bull age. Increasing age induces changes in sperm that reduce frozen semen quality. This study aimed to evaluate the frozen semen characteristics of Bali bulls aged 3, 8, and 13 years. Frozen semen was derived from fresh semen with sperm motility > 70%. The frozen semen characteristics tested included motility, viability, plasma membrane integrity, acrosome integrity, and sperm abnormalities. Sperm motility was analyzed by computer-assisted sperm analysis (CASA). Viability was assessed using eosin-nigrosin staining; plasma membrane integrity was assessed using the hypoosmotic swelling test (HOST); acrosome integrity was assessed using Giemsa staining; and sperm abnormalities were assessed using Williams staining. The plasma membrane integrity of the 13-year-old group was significantly lower (P < 0.05) than that of the younger age groups. However, there were no significant differences (P > 0.05) in the other parameters. We conclude that age affects the integrity of the sperm plasma membrane in frozen semen from Bali bulls.
Effects of Physalis angulata Leaf Extract on Female Reproductive Organs Following Busulfan Injection in Rats Satyaningtijas, Aryani; Ekastuti, Damiana Rita; Tarigan, Ronald; Prasetyaningtyas, Wahono Esthi; Adnyane, I Ketut Mudite; Mohamad, Kusdiantoro; Rahmadhani, Elsi; Sulistyana, Yanti; Mahendra, Arindina
HAYATI Journal of Biosciences Vol. 33 No. 2 (2026): March 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.2.490-498

Abstract

Physalis angulata L. (ciplukan) is traditionally used as an herbal remedy with anticancer and antioxidant properties. At the same time, busulfan, a chemotherapy alkylating agent, causes gonadotoxicity and oxidative stress that impair female reproduction. This study aimed to evaluate the effect of P. angulata on reproductive function after busulfan administration in female rats. Twenty-five 6-week-old female rats were randomly divided into: Group I (control), Group II (busulfan only), Group III (P. angulata only), Group IV (busulfan followed by P. angulata after 14 days), and Group V (busulfan and P. angulata administered for 28 days). FSHR and LHR expression in the ovaries and MDA levels in the ovaries and uterus were measured to assess reproductive changes. Busulfan showed no significant effect on FSHR and LHR, whereas P. angulata induced downregulation, suggesting a potential negative feedback mechanism on ovarian receptors. Ovarian MDA showed a decreasing trend with combined treatments, while uterine MDA peaked after busulfan but declined markedly with P. angulata, indicating its role in alleviating oxidative stress. In conclusion, P. angulata may modulate hormonal balance in rat ovaries and decrease oxidative stress in the uterus after busulfan treatment.
Kualitas Semen Cair Domba dalam Pengencer Komersial Berbasis Tris Kuning Telur dengan Penambahan Glutation Pangestu, Dyah Mekar; Prasetyaningtyas, Wahono Esthi; Fahrudi, Mokhamad; Karja, Ni Wayan Kurniani
Jurnal Sain Veteriner Vol 44, No 1 (2026): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.109646

Abstract

Penyimpanan semen cair berpotensi meningkatkan pembentukan radikal bebas dan stres oksidatif, yang menyebabkan kerusakan membran plasma spermatozoa dan penurunan kualitas semen. Glutation merupakan antioksidan yang mampu menetralkan radikal bebas dan mengurangi kerusakan oksidatif, sehingga berpotensi menjaga kualitas spermatozoa selama penyimpanan. Penelitian ini bertujuan mengevaluasi pengaruh penambahan glutation terhadap kualitas semen cair domba dalam pengencer komersial berbasis tris kuning telur. Semen segar dengan motilitas ≥70% diencerkan dengan penambahan glutation pada konsentrasi 0 mM (GSH-0), 2 mM (GSH-2), dan 4 mM (GSH-4), kemudian disimpan pada suhu 4  selama enam hari. Evaluasi dilakukan setiap 24 jam terhadap motilitas, viabilitas, keutuhan membran plasma (MPU), dan abnormalitas spermatozoa. Hasil hari keenam menunjukkan bahwa GSH-4 memberikan hasil terbaik, dengan motilitas 64,00 ± 2,24%, viabilitas 84,95 ± 1,12%, MPU 85,93 ± 0,46%, dan abnormalitas 9,47 ± 0,66%. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa penambahan glutation 4 mM efektif dalam mempertahankan kualitas semen cair domba selama pada suhu 
Co-Authors A.S. Satyaningtijas Aisyah Fidela Siregar Ali, Ihfan Khaidir Arief Boediono Aryani, Nursela Sofyanti Mirza Aulia Miftakhur Rahman Chairun Nisa Damiana Rita Ekastuti Dwitya Citraesti Edward, Syafri Ekayanti Mulyawati Kaiin Elmanaviean, Muhammad Erdiansyah Rahmi Erdiansyah Rahmi Fahrudi, Mokhamad Faisal Mustafa Gholib Gholib Gusdinar, Rizal Gustini Syahbirin Hamny Sofyan Hendriana, Okta Idawati Nasution Ita Djuwita ITA DJUWITA Ita Djuwita Kaiin, Ekayanti Mulyawati Kaiin, Ekayanti Mulyawati Karisma Mardatillah Ketut Adnyane Mudite Kusdiantoro Mohamad M Agus Setiadi Mahendra, Arindina Makhroja, Lalu Faris Naufal Mokhamad Fahrudin MUHAMMAD AGIL Muhammad Jalaluddin Muhammad Jalaluddin, Muhammad Muslim Akmal Ni Wayan Kurniani Karja Nugraha, Arifin Budiman Nugrahaeni, Mutiara Pangestu, Dyah Mekar Pattikawa, Vapriel Andhika R. Iis Arifiantini Rahmadhani, Elsi Ritonga, Mudhita Zikrullah Satrija, Esdinawan Carakantara Savitri Novelina Sri Estuningsih sri murtini . Sri Wahyuni Srihadi Agungpriyono Sulistyana, Yanti TAKDIR SAILI Tanjung, Fitri Aisah Tarigan, Ronald Taufan Hidayat Triyaningrum, Triyaningrum TUTY LASWARDI YUSUF Tuty Laswardi Yusuf Vetnizah Juniantito Wahyuni, Sri Wardhani, Lailia Dwi Kusuma Witri, Brilla Widya