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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Media Veteriner Jurnal Sain Veteriner Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Ilmu Ternak Veteriner Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) ANNALES BOGORIENSES Jurnal Medik Veteriner Jurnal Kedokteran Hewan Jurnal Bioteknologi & Biosains Indonesia (JBBI) Annales Bogorienses Jurnal Bioteknologi & Biosains Indonesia
Wahono Esthi Prasetyaningtyas
Departemen Anatomi, Fisiologi Dan Farmakologi Fakultas Kedokteran Hewan Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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Efek Campuran Ekstrak Temulawak (Curcuma xanthorrhiza) dan Temuputih (Curcuma zedoaria) terhadap Toksisitas Akut pada Embrio Ikan Zebra Mohamad, Kusdiantoro; Aryani, Nursela Sofyanti Mirza; Prasetyaningtyas, Wahono Esthi; Syahbirin, Gustini
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.94142

Abstract

Temulawak (Curcuma xanthorrhiza) dan temuputih (Curcuma zedoaria) termasuk tanaman herbal yang mempunyai potensi sebagai antitumor dan antikanker. Beberapa obat antikanker dan ekstrak herbal telah dilaporkan memiliki toksisitas yang tinggi dan menyebabkan malformasi pada embrio ikan zebra (Danio rerio). Penelitian ini bertujuan membandingkan toksisitas ekstrak etanol C. xanthorrhiza, C. zedoaria, dan campurannya (rasio 1:6) terhadap embrio ikan zebra. Rimpang C. xanthorrhiza dan C. zedoaria diekstrak dengan cara maserasi dalam etanol 80%. Ekstrak diuji toksisitas akut dengan embrio ikan zebra menggunakan metode OECD Guidelines for the Testing of Chemicals No. 236 (2013). Nilai lethal concentration 50 (LC50) campuran ekstrak adalah 82.9 ppm, berada di antara nilai LC50 masing-masing ekstrak (16.9 untuk C. xanthorrhiza dan 112.1 untuk C. zedoaria). Tingkat menetas embrio rendah pada ekstrak C. xanthorrhiza tetapi tinggi pada ekstrak C. zedoaria dan ekstrak campuran. Kelainan notokorda dan edema kantong kuning telur tinggi pada ekstrak C. xanthorrhiza tetapi rendah pada ekstrak C. zedoaria dan ekstrak campuran. Kelainan sirkulasi darah rendah pada ekstrak C. zedoaria tetapi tinggi pada ekstrak campuran. Edema perikardium dan koagulasi darah merupakan malformasi mayor pada semua ekstrak. Dapat disimpulkan bahwa campuran ekstrak etanol temulawak dan temuputih menyebabkan malformasi edema perikardium, koagulasi darah, dan secara sinergis menghambat sirkulasi darah, yang berkaitan dengan potensi antikanker. Selain itu, ekstrak campuran dapat menurunkan hambatan menetas, malformasi notokorda, dan edema kantong kuning telur.
Biological Analysis of Leydig Cells-Conditioned Medium to Support Rat Bone Marrow Mesenchymal Stem Cells Differentiation Kaiin, Ekayanti Mulyawati; Prasetyaningtyas, Wahono Esthi; Mohamad, Kusdiantoro; Djuwita, Ita; Yusuf, Tuty Laswardi; Setiadi, Mohamad Agus
Annales Bogorienses Vol. 22 No. 1 (2018): Annales Bogorienses
Publisher : BRIN

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Abstract

The developed Leydig cells-conditioned medium (LCM) contains bioactive materials secreted by Leydig cells in vitro. LCM was used to evaluate the ability of bone marrow mesenchymal stem cells differentiation. Bone marrow mesenchymal stem cells (1x 106 cell/ml) were cultured in : 1) DMEM supplemented with 10% NBCS as a control (M), 2) M supplemented with 10 ng/ml testosterone; 3) M supplemented with 50% LCM ; 4) M supplemented with 50% LCM and 2.5 IU/ml hCG. Bone marrow mesenchymal stem cells that were cultured with LCM has a positive reaction (57.4%) to histochemistry staining 3β-HSD and produced 1.87 ng/ml testosterone. Supplementation of hCG to LCM increased the positive number of Leydig cells and testosterone production by 74.6% and 12.33 ng/ml (P<0.05). It can be concluded that Leydig cells-conditioned medium can support differentiation of bone marrow mesenchymal stem cells into Leydig cells.
FROZEN SEMEN CHARACTERISTICS OF BALI BULL IN DIFFERENT AGE GROUPS Witri, Brilla Widya; Arifiantini, Iis; Prasetyaningtyas, Wahono Esthi; Karja, Ni Wayan Kurniani
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.10087

Abstract

One factor that affects frozen semen characteristics is bull age. Increasing age induces changes in sperm that reduce frozen semen quality. This study aimed to evaluate the frozen semen characteristics of Bali bulls aged 3, 8, and 13 years. Frozen semen was derived from fresh semen with sperm motility > 70%. The frozen semen characteristics tested included motility, viability, plasma membrane integrity, acrosome integrity, and sperm abnormalities. Sperm motility was analyzed by computer-assisted sperm analysis (CASA). Viability was assessed using eosin-nigrosin staining; plasma membrane integrity was assessed using the hypoosmotic swelling test (HOST); acrosome integrity was assessed using Giemsa staining; and sperm abnormalities were assessed using Williams staining. The plasma membrane integrity of the 13-year-old group was significantly lower (P < 0.05) than that of the younger age groups. However, there were no significant differences (P > 0.05) in the other parameters. We conclude that age affects the integrity of the sperm plasma membrane in frozen semen from Bali bulls.
Effects of Physalis angulata Leaf Extract on Female Reproductive Organs Following Busulfan Injection in Rats Satyaningtijas, Aryani; Ekastuti, Damiana Rita; Tarigan, Ronald; Prasetyaningtyas, Wahono Esthi; Adnyane, I Ketut Mudite; Mohamad, Kusdiantoro; Rahmadhani, Elsi; Sulistyana, Yanti; Mahendra, Arindina
HAYATI Journal of Biosciences Vol. 33 No. 2 (2026): March 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.2.490-498

Abstract

Physalis angulata L. (ciplukan) is traditionally used as an herbal remedy with anticancer and antioxidant properties. At the same time, busulfan, a chemotherapy alkylating agent, causes gonadotoxicity and oxidative stress that impair female reproduction. This study aimed to evaluate the effect of P. angulata on reproductive function after busulfan administration in female rats. Twenty-five 6-week-old female rats were randomly divided into: Group I (control), Group II (busulfan only), Group III (P. angulata only), Group IV (busulfan followed by P. angulata after 14 days), and Group V (busulfan and P. angulata administered for 28 days). FSHR and LHR expression in the ovaries and MDA levels in the ovaries and uterus were measured to assess reproductive changes. Busulfan showed no significant effect on FSHR and LHR, whereas P. angulata induced downregulation, suggesting a potential negative feedback mechanism on ovarian receptors. Ovarian MDA showed a decreasing trend with combined treatments, while uterine MDA peaked after busulfan but declined markedly with P. angulata, indicating its role in alleviating oxidative stress. In conclusion, P. angulata may modulate hormonal balance in rat ovaries and decrease oxidative stress in the uterus after busulfan treatment.
Co-Authors A.S. Satyaningtijas Aisyah Fidela Siregar Ali, Ihfan Khaidir Arief Boediono Aryani, Nursela Sofyanti Mirza Aulia Miftakhur Rahman Chairun Nisa Damiana Rita Ekastuti Dwitya Citraesti Edward, Syafri Ekayanti Mulyawati Kaiin Elmanaviean, Muhammad Erdiansyah Rahmi Erdiansyah Rahmi Faisal Mustafa Gholib Gholib Gusdinar, Rizal Gustini Syahbirin Hamny Sofyan Hendriana, Okta Idawati Nasution Ita Djuwita ITA DJUWITA Ita Djuwita Kaiin, Ekayanti Mulyawati Kaiin, Ekayanti Mulyawati Karisma Mardatillah Ketut Adnyane Mudite Kusdiantoro Mohamad M Agus Setiadi Mahendra, Arindina Makhroja, Lalu Faris Naufal Mokhamad Fahrudin MUHAMMAD AGIL Muhammad Jalaluddin Muhammad Jalaluddin, Muhammad Muslim Akmal Ni Wayan Kurniani Karja Nugraha, Arifin Budiman Nugrahaeni, Mutiara Pattikawa, Vapriel Andhika R. Iis Arifiantini Rahmadhani, Elsi Ritonga, Mudhita Zikrullah Satrija, Esdinawan Carakantara Savitri Novelina Sri Estuningsih sri murtini . Sri Wahyuni Srihadi Agungpriyono Sulistyana, Yanti TAKDIR SAILI Tanjung, Fitri Aisah Tarigan, Ronald Taufan Hidayat Triyaningrum, Triyaningrum TUTY LASWARDI YUSUF Tuty Laswardi Yusuf Vetnizah Juniantito Wahyuni, Sri Wardhani, Lailia Dwi Kusuma Witri, Brilla Widya